Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Reverse (-)

30032495 .. 30039054

Gene Structure 6560 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008399

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGGAAAATTATGGCTTATCATCACCCTCCTCTTCCTCCCTTGCTTCTTCATAAGCCACGACGTCGTTGGCGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTTCGAATCCGGTGACCACCTTAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGCGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCCGAAATTGTCGAGGTAGAGGAGAATTCAAGCAACAAGATGCAAACTTTTCGAATACCACCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACGACCTTACGTACATTCGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCGGATGCAAAAGTTGTCAAGATATGTGAGAGGTTACGAGATGAAAATGGCACCATTATTGAGCATACAGAGTTAGCTTTTCCACACTATTTACCAATGTGCTGTCCTTGTGGGCCTCAGTGGCGGGTACCTTCATCATGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCGAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATTGGACAGCGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGGATCCAAAGTTTCGGAAGTGATTGTAGGTCCGGAAAACAGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGATTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGTGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAATTATGGAATTTCTTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAAAATACTGGTGAAGTAGAAGCATCCTATTGCTTAACGTTTGATTGTCCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAATGTTAACTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCTGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGAGCCGAGTGCCAATTTTCTACAATGTCTACTGTCATTGATAATGGATCACAGGTTAATCCCTTTAAGCCACCAAAGACAGGAACAACTGGTTTCTTTGACTCCCTTGAAAGCTTTTGGAACCATGCGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTTGGTCTAGTTTTGGCAATTTTTCCAACAGTCATTGCGCTACTGTGGCTTTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGCTGGGATGATCATTTCTGGGAGGATAGTCCAAGCACCAAGAATACAAGACACAGAATTAATGTAGATCACCGACATGTTCATGTCCATAAACATCATGGGCATGAAGCAAGGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGATTACCATTACGACCTTCACCATGTCCATAAAGATAAGCACAAGCATGGCCGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCACGGCCAGAAAAAACAAAAGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

712

Amino Acids

81.27

Weight (kDa)

8.1

Isoelectric Point (pI)

41.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	46 - 565	9.7e-155	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1458
AatII	GACGTC	1 cut(s)	66
Acc65I	GGTACC	1 cut(s)	514
AccB1I	GGYRCC	4 cut(s)	437, 514, 1039, 1911
AccB7I	CCANNNNNTGG	2 cut(s)	838, 1049
AccI	GTMKAC	2 cut(s)	1182, 1499
AccII	CGCG	1 cut(s)	185
AccIII	TCCGGA	1 cut(s)	694
AciI	CCGC	5 cut(s)	180, 294, 389, 511, 583
AclI	AACGTT	1 cut(s)	1307
AclWI	GGATC	5 cut(s)	73, 660, 673, 1525, 1778
AcoI	YGGCCR	3 cut(s)	168, 2038, 2092
AcsI	RAATTY	3 cut(s)	235, 835, 997
AcuI	CTGAAG	2 cut(s)	369, 1651
AcyI	GRCGYC	2 cut(s)	63, 72
AfaI	GTAC	5 cut(s)	311, 323, 356, 516, 1685
AfeI	AGCGCT	1 cut(s)	626
AfiI	CCNNNNNNNGG	8 cut(s)	166, 589, 812, 838, 1049, 1094, 1339, 1550
AflII	CTTAAG	2 cut(s)	1207, 1442
AflIII	ACRYGT	1 cut(s)	1873
AgsI	TTSAA	8 cut(s)	240, 653, 902, 1390, 1580, 1979, 2052, 2130
AjnI	CCWGG	3 cut(s)	820, 1195, 1399
AloI	GAACNNNNNNTCC	4 cut(s)	627, 659, 1049, 1081
AluBI	AGCT	5 cut(s)	299, 464, 737, 1415, 1584
AluI	AGCT	5 cut(s)	299, 464, 737, 1415, 1584
AlwI	GGATC	5 cut(s)	73, 660, 673, 1525, 1778
Aor13HI	TCCGGA	1 cut(s)	694
Aor51HI	AGCGCT	1 cut(s)	626
AoxI	GGCC	6 cut(s)	168, 500, 817, 1233, 2038, 2092
ApaI	GGGCCC	1 cut(s)	821
ApeKI	GCWGC	5 cut(s)	296, 865, 943, 1415, 1433
ApoI	RAATTY	3 cut(s)	235, 835, 997
AseI	ATTAAT	2 cut(s)	1134, 1857
Asp700I	GAANNNNTTC	2 cut(s)	116, 2047
Asp718I	GGTACC	1 cut(s)	514
AspLEI	GCGC	3 cut(s)	185, 627, 1750
AspS9I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
AsuC2I	CCSGG	1 cut(s)	591
AsuHPI	GGTGA	9 cut(s)	16, 140, 206, 605, 954, 1292, 1321, 1859, 2000
AsuII	TTCGAA	3 cut(s)	120, 262, 1068
AvaII	GGWCC	1 cut(s)	692
BaeGI	GKGCMC	1 cut(s)	821
BamHI	GGATCC	1 cut(s)	665
BanI	GGYRCC	4 cut(s)	437, 514, 1039, 1911
BanII	GRGCYC	1 cut(s)	821
BbsI	GAAGAC	2 cut(s)	119, 1610
BbvI	GCAGC	5 cut(s)	308, 852, 955, 1402, 1420
BccI	CCATC	1 cut(s)	887
BceAI	ACGGC	2 cut(s)	155, 2107
BcgI	CGANNNNNNTGC	4 cut(s)	242, 276, 1397, 1431
BciT130I	CCWGG	3 cut(s)	822, 1197, 1401
BciVI	GTATCC	2 cut(s)	755, 2078
BclI	TGATCA	2 cut(s)	1807, 1957
BcnI	CCSGG	1 cut(s)	591
BfaI	CTAG	2 cut(s)	177, 1718
BfoI	RGCGCY	1 cut(s)	628
BfrI	CTTAAG	2 cut(s)	1207, 1442
BfuI	GTATCC	2 cut(s)	755, 2078
BglI	GCCNNNNNGGC	1 cut(s)	508
BisI	GCNGC	6 cut(s)	181, 297, 866, 944, 1416, 1434
BlsI	GCNGC	6 cut(s)	182, 298, 867, 945, 1417, 1435
BmcAI	AGTACT	1 cut(s)	356
Bme1390I	CCNGG	4 cut(s)	591, 822, 1197, 1401
Bme18I	GGWCC	1 cut(s)	692
BmgT120I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
BmrFI	CCNGG	4 cut(s)	591, 822, 1197, 1401
BmsI	GCATC	8 cut(s)	240, 376, 382, 852, 1093, 1221, 1301, 1661
BpiI	GAAGAC	2 cut(s)	119, 1610
BpmI	CTGGAG	2 cut(s)	890, 991
Bpu14I	TTCGAA	3 cut(s)	120, 262, 1068
BpuMI	CCSGG	1 cut(s)	591
BsaAI	YACGTR	1 cut(s)	321
BsaHI	GRCGYC	2 cut(s)	63, 72
BsaJI	CCNNGG	5 cut(s)	806, 820, 1196, 1399, 2089
BsaWI	WCCGGW	2 cut(s)	125, 694
BsaXI	ACNNNNNCTCC	4 cut(s)	741, 771, 2038, 2068
Bsc4I	CCNNNNNNNGG	8 cut(s)	166, 589, 812, 838, 1049, 1094, 1339, 1550
Bse1I	ACTGG	4 cut(s)	1282, 1564, 1630, 2086
Bse3DI	GCAATG	1 cut(s)	1743
BseAI	TCCGGA	1 cut(s)	694
BseBI	CCWGG	3 cut(s)	822, 1197, 1401
BseDI	CCNNGG	5 cut(s)	806, 820, 1196, 1399, 2089
BseGI	GGATG	5 cut(s)	397, 867, 1084, 1292, 1810
BseLI	CCNNNNNNNGG	8 cut(s)	166, 589, 812, 838, 1049, 1094, 1339, 1550
BseMI	GCAATG	1 cut(s)	1743
BseMII	CTCAG	2 cut(s)	518, 642
BseNI	ACTGG	4 cut(s)	1282, 1564, 1630, 2086
BseRI	GAGGAG	3 cut(s)	20, 245, 1355
BseSI	GKGCMC	1 cut(s)	821
BseXI	GCAGC	5 cut(s)	308, 852, 955, 1402, 1420
BseYI	CCCAGC	1 cut(s)	1800
BsgI	GTGCAG	1 cut(s)	127
Bsh1236I	CGCG	1 cut(s)	185
BshFI	GGCC	6 cut(s)	170, 502, 819, 1235, 2040, 2094
BshNI	GGYRCC	4 cut(s)	437, 514, 1039, 1911
BsiSI	CCGG	3 cut(s)	126, 590, 695
BslFI	GGGAC	3 cut(s)	158, 1111, 1788
BslI	CCNNNNNNNGG	8 cut(s)	166, 589, 812, 838, 1049, 1094, 1339, 1550
BsmFI	GGGAC	3 cut(s)	158, 1111, 1788
BsnI	GGCC	6 cut(s)	170, 502, 819, 1235, 2040, 2094
Bsp119I	TTCGAA	3 cut(s)	120, 262, 1068
Bsp120I	GGGCCC	1 cut(s)	817
Bsp1286I	GDGCHC	1 cut(s)	821
Bsp13I	TCCGGA	1 cut(s)	694
BspACI	CCGC	5 cut(s)	180, 294, 389, 511, 583
BspANI	GGCC	6 cut(s)	170, 502, 819, 1235, 2040, 2094
BspCNI	CTCAG	2 cut(s)	517, 641
BspEI	TCCGGA	1 cut(s)	694
BspFNI	CGCG	1 cut(s)	185
BspPI	GGATC	5 cut(s)	73, 660, 673, 1525, 1778
BspQI	GCTCTTC	1 cut(s)	964
BspT104I	TTCGAA	3 cut(s)	120, 262, 1068
BspT107I	GGYRCC	4 cut(s)	437, 514, 1039, 1911
BspTI	CTTAAG	2 cut(s)	1207, 1442
BsrDI	GCAATG	1 cut(s)	1743
BsrI	ACTGG	4 cut(s)	1282, 1564, 1630, 2086
BssECI	CCNNGG	5 cut(s)	806, 820, 1196, 1399, 2089
BssNI	GRCGYC	2 cut(s)	63, 72
Bst2UI	CCWGG	3 cut(s)	822, 1197, 1401
Bst4CI	ACNGT	6 cut(s)	283, 1022, 1504, 1683, 1741, 1755
Bst6I	CTCTTC	3 cut(s)	38, 345, 964
BstACI	GRCGYC	2 cut(s)	63, 72
BstAFI	CTTAAG	2 cut(s)	1207, 1442
BstAPI	GCANNNNNTGC	1 cut(s)	302
BstBAI	YACGTR	1 cut(s)	321
BstBI	TTCGAA	3 cut(s)	120, 262, 1068
BstC8I	GCNNGC	2 cut(s)	735, 980
BstDEI	CTNAG	4 cut(s)	147, 504, 628, 1157
BstDSI	CCRYGG	1 cut(s)	2089
BstEII	GGTNACC	1 cut(s)	128
BstENI	CCTNNNNNAGG	1 cut(s)	1337
BstF5I	GGATG	5 cut(s)	397, 867, 1084, 1292, 1810
BstFNI	CGCG	1 cut(s)	185
BstH2I	RGCGCY	1 cut(s)	628
BstHHI	GCGC	3 cut(s)	185, 627, 1750
BstMWI	GCNNNNNNNGC	3 cut(s)	302, 508, 1756
BstNI	CCWGG	3 cut(s)	822, 1197, 1401
BstNSI	RCATGY	1 cut(s)	1877
BstPI	GGTNACC	1 cut(s)	128
BstSCI	CCNGG	4 cut(s)	589, 820, 1195, 1399
BstSLI	GKGCMC	1 cut(s)	821
BstSNI	TACGTA	1 cut(s)	321
BstUI	CGCG	1 cut(s)	185
BstV1I	GCAGC	5 cut(s)	308, 852, 955, 1402, 1420
BstV2I	GAAGAC	2 cut(s)	119, 1610
BstX2I	RGATCY	2 cut(s)	78, 665
BstXI	CCANNNNNNTGG	2 cut(s)	611, 1922
BstYI	RGATCY	2 cut(s)	78, 665
BsuI	GTATCC	2 cut(s)	755, 2078
BsuRI	GGCC	6 cut(s)	170, 502, 819, 1235, 2040, 2094
BtgI	CCRYGG	1 cut(s)	2089
BtsCI	GGATG	5 cut(s)	397, 867, 1084, 1292, 1810
BtsIMutI	CAGTG	4 cut(s)	512, 636, 1623, 2079
Cac8I	GCNNGC	2 cut(s)	735, 980
CfoI	GCGC	3 cut(s)	185, 627, 1750
Cfr13I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
CseI	GACGC	1 cut(s)	61
Csp6I	GTAC	5 cut(s)	310, 322, 355, 515, 1684
CviQI	GTAC	5 cut(s)	310, 322, 355, 515, 1684
DdeI	CTNAG	4 cut(s)	147, 504, 628, 1157
EaeI	YGGCCR	3 cut(s)	168, 2038, 2092
Eam1104I	CTCTTC	3 cut(s)	38, 345, 964
EarI	CTCTTC	3 cut(s)	38, 345, 964
Eco105I	TACGTA	1 cut(s)	321
Eco24I	GRGCYC	1 cut(s)	821
Eco47I	GGWCC	1 cut(s)	692
Eco47III	AGCGCT	1 cut(s)	626
Eco57I	CTGAAG	2 cut(s)	369, 1651
Eco91I	GGTNACC	1 cut(s)	128
EcoNI	CCTNNNNNAGG	1 cut(s)	1337
EcoO109I	RGGNCCY	2 cut(s)	817, 1234
EcoO65I	GGTNACC	1 cut(s)	128
EcoRI	GAATTC	1 cut(s)	235
EcoRII	CCWGG	3 cut(s)	820, 1195, 1399
EcoT38I	GRGCYC	1 cut(s)	821
FaqI	GGGAC	3 cut(s)	158, 1111, 1788
FauI	CCCGC	1 cut(s)	504
FauNDI	CATATG	1 cut(s)	1688
FbaI	TGATCA	2 cut(s)	1807, 1957
FblI	GTMKAC	2 cut(s)	1182, 1499
Fnu4HI	GCNGC	6 cut(s)	181, 297, 866, 944, 1416, 1434
FokI	GGATG	5 cut(s)	404, 874, 1071, 1279, 1817
FriOI	GRGCYC	1 cut(s)	821
Fsp4HI	GCNGC	6 cut(s)	181, 297, 866, 944, 1416, 1434
FspBI	CTAG	2 cut(s)	177, 1718
GlaI	GCGC	3 cut(s)	184, 626, 1749
GluI	GCNGC	6 cut(s)	181, 297, 866, 944, 1416, 1434
GsaI	CCCAGC	1 cut(s)	1804
GsuI	CTGGAG	2 cut(s)	890, 991
HaeII	RGCGCY	1 cut(s)	628
HaeIII	GGCC	6 cut(s)	170, 502, 819, 1235, 2040, 2094
HapII	CCGG	3 cut(s)	126, 590, 695
HgaI	GACGC	1 cut(s)	61
HhaI	GCGC	3 cut(s)	185, 627, 1750
Hin1I	GRCGYC	2 cut(s)	63, 72
Hin6I	GCGC	3 cut(s)	183, 625, 1748
HinP1I	GCGC	3 cut(s)	183, 625, 1748
HincII	GTYRAC	3 cut(s)	827, 922, 1377
HindII	GTYRAC	3 cut(s)	827, 922, 1377
HindIII	AAGCTT	1 cut(s)	1582
HinfI	GANTC	8 cut(s)	122, 1099, 1119, 1192, 1448, 1571, 1656, 1702
HpaI	GTTAAC	1 cut(s)	1377
HpaII	CCGG	3 cut(s)	126, 590, 695
HphI	GGTGA	9 cut(s)	16, 140, 206, 605, 954, 1292, 1321, 1859, 2000
Hpy166II	GTNNAC	5 cut(s)	827, 922, 1183, 1377, 1500
Hpy188I	TCNGA	5 cut(s)	679, 807, 1670, 1707, 1770
Hpy188III	TCNNGA	8 cut(s)	76, 277, 329, 406, 695, 970, 1147, 1653
Hpy8I	GTNNAC	5 cut(s)	827, 922, 1183, 1377, 1500
Hpy99I	CGWCG	2 cut(s)	65, 68
HpyAV	CCTTC	8 cut(s)	528, 786, 837, 1052, 1056, 1765, 2015, 2037
HpyCH4III	ACNGT	6 cut(s)	283, 1022, 1504, 1683, 1741, 1755
HpyCH4IV	ACGT	4 cut(s)	63, 320, 1170, 1307
HpyCH4V	TGCA	9 cut(s)	144, 253, 338, 395, 982, 1094, 1418, 1638, 2062
HpyF10VI	GCNNNNNNNGC	3 cut(s)	302, 508, 1756
HpyF3I	CTNAG	4 cut(s)	147, 504, 628, 1157
HpySE526I	ACGT	4 cut(s)	63, 320, 1170, 1307
Hsp92I	GRCGYC	2 cut(s)	63, 72
HspAI	GCGC	3 cut(s)	183, 625, 1748
Kpn2I	TCCGGA	1 cut(s)	694
KpnI	GGTACC	1 cut(s)	518
Ksp22I	TGATCA	2 cut(s)	1807, 1957
KspAI	GTTAAC	1 cut(s)	1377
LguI	GCTCTTC	1 cut(s)	964
LmnI	GCTCC	1 cut(s)	1342
Lsp1109I	GCAGC	5 cut(s)	308, 852, 955, 1402, 1420
LweI	GCATC	8 cut(s)	240, 376, 382, 852, 1093, 1221, 1301, 1661
MaeI	CTAG	2 cut(s)	177, 1718
MaeII	ACGT	4 cut(s)	63, 320, 1170, 1307
MaeIII	GTNAC	4 cut(s)	128, 420, 1120, 1327
MboII	GAAGA	7 cut(s)	25, 40, 124, 163, 362, 951, 1615
MflI	RGATCY	2 cut(s)	78, 665
MhlI	GDGCHC	1 cut(s)	821
MlyI	GAGTC	5 cut(s)	1093, 1128, 1201, 1442, 1565
MmeI	TCCRAC	3 cut(s)	739, 1679, 1760
MroI	TCCGGA	1 cut(s)	694
MroXI	GAANNNNTTC	2 cut(s)	116, 2047
MslI	CAYNNNNRTG	8 cut(s)	1089, 1334, 1691, 1872, 1878, 1899, 1920, 2034
MspCI	CTTAAG	2 cut(s)	1207, 1442
MspI	CCGG	3 cut(s)	126, 590, 695
MspR9I	CCNGG	4 cut(s)	591, 822, 1197, 1401
MvaI	CCWGG	3 cut(s)	822, 1197, 1401
MvnI	CGCG	1 cut(s)	185
MwoI	GCNNNNNNNGC	3 cut(s)	302, 508, 1756
NciI	CCSGG	1 cut(s)	591
NdeI	CATATG	1 cut(s)	1688
NmeAIII	GCCGAG	1 cut(s)	1501
NmuCI	GTSAC	3 cut(s)	128, 1120, 1327
NspI	RCATGY	1 cut(s)	1877
NspV	TTCGAA	3 cut(s)	120, 262, 1068
PciI	ACATGT	1 cut(s)	1873
PciSI	GCTCTTC	1 cut(s)	964
PcsI	WCGNNNNNNNCGW	2 cut(s)	69, 105
PdmI	GAANNNNTTC	2 cut(s)	116, 2047
PfeI	GAWTC	3 cut(s)	122, 1656, 1702
PflMI	CCANNNNNTGG	2 cut(s)	838, 1049
PkrI	GCNGC	6 cut(s)	182, 298, 867, 945, 1417, 1435
PleI	GAGTC	5 cut(s)	1093, 1127, 1200, 1442, 1565
PpsI	GAGTC	5 cut(s)	1093, 1127, 1200, 1442, 1565
Ppu21I	YACGTR	1 cut(s)	321
PscI	ACATGT	1 cut(s)	1873
PshBI	ATTAAT	2 cut(s)	1134, 1857
PsiI	TTATAA	1 cut(s)	1458
Psp1406I	AACGTT	1 cut(s)	1307
Psp6I	CCWGG	3 cut(s)	820, 1195, 1399
PspEI	GGTNACC	1 cut(s)	128
PspFI	CCCAGC	1 cut(s)	1800
PspGI	CCWGG	3 cut(s)	820, 1195, 1399
PspOMI	GGGCCC	1 cut(s)	817
PspPI	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
PsrI	GAACNNNNNNTAC	2 cut(s)	2069, 2101
PsuI	RGATCY	2 cut(s)	78, 665
RsaI	GTAC	5 cut(s)	311, 323, 356, 516, 1685
RsaNI	GTAC	5 cut(s)	310, 322, 355, 515, 1684
RseI	CAYNNNNRTG	8 cut(s)	1089, 1334, 1691, 1872, 1878, 1899, 1920, 2034
SapI	GCTCTTC	1 cut(s)	964
SatI	GCNGC	6 cut(s)	181, 297, 866, 944, 1416, 1434
Sau96I	GGNCC	5 cut(s)	500, 692, 817, 818, 1234
ScaI	AGTACT	1 cut(s)	356
SchI	GAGTC	5 cut(s)	1093, 1128, 1201, 1442, 1565
ScrFI	CCNGG	4 cut(s)	591, 822, 1197, 1401
SduI	GDGCHC	1 cut(s)	821
SfaNI	GCATC	8 cut(s)	240, 376, 382, 852, 1093, 1221, 1301, 1661
SfuI	TTCGAA	3 cut(s)	120, 262, 1068
SinI	GGWCC	1 cut(s)	692
SmiMI	CAYNNNNRTG	8 cut(s)	1089, 1334, 1691, 1872, 1878, 1899, 1920, 2034
SmlI	CTYRAG	2 cut(s)	1207, 1442
SmoI	CTYRAG	2 cut(s)	1207, 1442
SnaBI	TACGTA	1 cut(s)	321
SsiI	CCGC	5 cut(s)	180, 294, 389, 511, 583
SspI	AATATT	1 cut(s)	772
SspMI	CTAG	2 cut(s)	177, 1718
StyD4I	CCNGG	4 cut(s)	589, 820, 1195, 1399
TaaI	ACNGT	6 cut(s)	283, 1022, 1504, 1683, 1741, 1755
TaiI	ACGT	4 cut(s)	66, 323, 1173, 1310
TatI	WGTACW	2 cut(s)	354, 1683
TauI	GCSGC	1 cut(s)	183
TfiI	GAWTC	3 cut(s)	122, 1656, 1702
TscAI	CASTG	4 cut(s)	512, 636, 1630, 2086
TseFI	GTSAC	3 cut(s)	128, 1120, 1327
TseI	GCWGC	5 cut(s)	296, 865, 943, 1415, 1433
Tsp45I	GTSAC	3 cut(s)	128, 1120, 1327
TspRI	CASTG	4 cut(s)	512, 636, 1630, 2086
Van91I	CCANNNNNTGG	2 cut(s)	838, 1049
Vha464I	CTTAAG	2 cut(s)	1207, 1442
VpaK11BI	GGWCC	1 cut(s)	692
VspI	ATTAAT	2 cut(s)	1134, 1857
XagI	CCTNNNNNAGG	1 cut(s)	1337
XapI	RAATTY	3 cut(s)	235, 835, 997
XceI	RCATGY	1 cut(s)	1877
XcmI	CCANNNNNNNNNTGG	3 cut(s)	65, 1193, 1892
XmiI	GTMKAC	2 cut(s)	1182, 1499
XmnI	GAANNNNTTC	2 cut(s)	116, 2047
XspI	CTAG	2 cut(s)	177, 1718
ZraI	GACGTC	1 cut(s)	64
ZrmI	AGTACT	1 cut(s)	356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.