Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0004191

Physical Location & Seq

Genomic Coordinates Forward (+)

31723470 .. 31730020

Gene Structure 6551 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G013580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2217 bp

ATGAATTTTTCCTTATTTGATTTCGGATCTTCCTCTGGAGATGTTACAATCTTTTCAGCTTTTCATTATTCTGTTTTCTCCCAGACATTCCCAAAAATGGGAAACCCTCCACCAAAATTATGGCTTATCACCCTCATCTTCCTCCCTTGCTTCTTCATAAGCGACGTCGTTGGAGTCCAGATCCTCTCCAAATCGAAACTCGACAAGTGCGAGAAGACTTCCGAATCCGGTGAACTTAACTGCACTAAGAAGATCGTCCTCAACATGGCCGTCCCTAGTGGCGCGAGTGGAGGTGAGGCTTCGATTGTTGCGGAAATTGTCGAGGTAGAGGAGAATTCAAGCAAGAAGATGCAAACTTTTCGAATACCACCTGTCCTGACTGTCAATAAATCCGCAGCTTATGCCTTGTACGAGCTTACGTACATTAGAGATGTTGCATATAAGCCTGAAGAGTACTATGTTAAGACCCGAAAGTGTGAGCCAGATGCAGATGCAAAAGTTGTCAAGATATGTGAGAGGTTACGAGATGAAAATGGTACCATTATTGAACATACAGAGCCAATGTGCTGTCCTTGTGGGCCTCAGCGGCGGGTACCTTCATCTTGTGGAAGTGTTTTTGACAAGTTGATGAAAGGAAAACCGAATACAGCCCATTGTGTCCGCTTTCCGGGTGATTGGTTCCATGTTTTTGGTATCGGACAACGCTCAGTGGGATTTAGTGTTCTCATTCAAGTAAAGACAGGATCTAAAGTTTCGGAAGTGCTTGTAGGTCCTGAAAACAGAACAGCAATATCCAATGATAAGTTTTTGCGAGCTAATCTTATTGGAGACTTTGTTGGATACACAAATATTCCTTCATTCGAGGATTTTTACCTTGTAATTCCGAGGCAGGGGGGCCCAGGTCAACCCCAGAATTTGGGAAGGAACTTTTCTATGTGGATGCTGCTGGAGAGAGTTAGATTTACTTTAGATGGTGTTGAATGTAATAAAATTGGCGTCAACTTTGAGGCTTTTAATGGGCAGCCAAACTTTTGCTCTTCACCATTCTGGAGTTGCTTGCATAATCAATTATGGAATTTCCTGGAGGCTGACCAAAACCGTATCGAAAGGAAACAGGTGCCACTTTTTGGTGTGGAAGGAAGGTTCGAAAGGATAAACCAGCATCCAAATGCAGGGACTCATTCGTTTTCCATAGGAGTCACAGAAGTCATTAATACAAATCTCTTGATAGAACTAAGTGCTGATGACGTAGATTATGTCTACCAAAGGAGTCCTGGGAAAATCTTAAGCATCAATATCCCAACATTTGAGGCCCTTACTCAATTTGGAACTGCTACAATCACAGCAAAAAATACTGGTGAAGTAGAAGCATCCTATTGCTTAACGTTTGATTGTTCAAGTGATGTCACCCTTATGGAGGAGCAATACTTCATTATGAAGCCAAAAGAAATGTTAACTCGATCATTCAAACTTTACCCAGGAACCGATCAAGCTGCAAAATACATCTGTGCAGCTATACTTAAGGACTCTAATTATAATGAAGTGGATAGAGCCGAGTGCCAATTTTCTACAACGTCTACTGTCATTGATAATGGATCACAGGTTAATCCCTTTAAGCCACCAAAGACAGGAACAACTGGTTTCTTTGACTCCATTGAAAGCTTTTGGAACCATGCGTGGAAGACTTTTATAGATTTCATCACTGGAAAATCTTGCAGAACAAAATGCTCTGGATTCTTTGACTTCAGATGCCATATACAGTACATATGTATGAGTTGGATTCTGATGTTTGGTCTACTTTTGGCAATTTTTCCAACAGTCATTGTGCTTCTGTGGCTTTTACATCAGAAGGGACTTTTTGATCCTTTATATGACTGGTGGGATGATCATTTCTGGGAAGATAGTCCGAGCACCAAGAATACTAGACACAGAATTAATGTAGATCATCAACATGTTCATGTCCATAAACATCATGGGCATGAAACAAGGCACCACAACTATGGCACTCATCATAAAAGAAAAAGCACTCACAATGATCACAAGCATAGCCACTTTCAAAGAAGTAGTGATGACCATTACGACCTTCACCATGTCCATAAGGATAAGCACAAGCATAGCCGAAGAAGGAGTTCAAGTGTTATGCAGAAAGTTGATGAGAACACTGGATACCACGGCCAGAAAAAACAAAGGAGAACAACAAAAGACTATTCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005887 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009566 GO:0009567 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0045026 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046 GO:0061024 GO:0061025 GO:0061936 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HAP2-GCS1

Protein Analysis

738

Amino Acids

84.24

Weight (kDa)

8.11

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAP2-GCS1	PF10699	79 - 591	1.6e-160	Male gamete fusion factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11720
fragaria_vesca	FvH4_5g01650 FvH4_5g28890
malus_domestica	MD04G1066100.v1.1
prunus_persica	Prupe.5G092400_v2.0.a1 Prupe.5G092400_v2.0.a1 Prupe.8G039400_v2.0.a1 Prupe.8G039500_v2.0.a1 Prupe.8G039500_v2.0.a1
pyrus_communis	pycom04g05950
rosa_chinensis	RchiOBHm_Chr4g0410761 RchiOBHm_Chr7g0199071
rosa_laevigata	RLG00000003818 RLG00000008399
rosa_multiflora	Rmu_sc0009499.1_g000004 Rmu_sc0021158.1_g000001
rosa_roxburghii	Rroxscaffold_3G00257150 Rroxscaffold_5G00355510
rosa_rugosa	Rorug04G0111100 Rorug04G0111200 Rorug07G0052400 Rorug07G0052500
rosa_samantha	Rh4AG162900 Rh4BG161700 Rh4BG162100 Rh4CG175700 Rh4DG158600 Rh7AG175800 Rh7BG179000 Rh7CG184900 Rh7DG178600
rosa_wichuraiana	Rw4G013580 Rw7G015420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1536
AatII	GACGTC	1 cut(s)	168
Acc65I	GGTACC	2 cut(s)	536, 592
AccB1I	GGYRCC	4 cut(s)	536, 592, 1117, 1989
AccB7I	CCANNNNNTGG	2 cut(s)	916, 1127
AccI	GTMKAC	3 cut(s)	1260, 1577, 1795
AccII	CGCG	1 cut(s)	284
AciI	CCGC	5 cut(s)	311, 393, 586, 589, 661
AclI	AACGTT	1 cut(s)	1385
AclWI	GGATC	5 cut(s)	34, 175, 751, 1603, 1856
AcoI	YGGCCR	2 cut(s)	267, 2173
AcsI	RAATTY	4 cut(s)	4, 334, 913, 1075
AcuI	CTGAAG	2 cut(s)	468, 1729
AcyI	GRCGYC	2 cut(s)	165, 996
AfaI	GTAC	6 cut(s)	410, 422, 455, 538, 594, 1763
AflII	CTTAAG	2 cut(s)	1285, 1520
AflIII	ACRYGT	1 cut(s)	1951
AjnI	CCWGG	4 cut(s)	898, 1080, 1273, 1477
AloI	GAACNNNNNNTCC	4 cut(s)	705, 737, 1127, 1159
AluBI	AGCT	7 cut(s)	59, 398, 415, 815, 1493, 1514, 1662
AluI	AGCT	7 cut(s)	59, 398, 415, 815, 1493, 1514, 1662
Alw21I	GWGCWC	1 cut(s)	1913
Alw26I	GTCTC	1 cut(s)	822
AlwI	GGATC	5 cut(s)	34, 175, 751, 1603, 1856
AoxI	GGCC	5 cut(s)	267, 578, 895, 1311, 2173
ApaI	GGGCCC	1 cut(s)	899
ApeKI	GCWGC	5 cut(s)	395, 943, 1021, 1493, 1511
ApoI	RAATTY	4 cut(s)	4, 334, 913, 1075
AseI	ATTAAT	2 cut(s)	1212, 1935
Asp700I	GAANNNNTTC	1 cut(s)	2128
Asp718I	GGTACC	2 cut(s)	536, 592
AspLEI	GCGC	1 cut(s)	284
AspS9I	GGNCC	5 cut(s)	578, 770, 895, 896, 1312
AsuC2I	CCSGG	1 cut(s)	669
AsuHPI	GGTGA	8 cut(s)	121, 242, 305, 683, 1032, 1370, 1399, 2078
AsuII	TTCGAA	2 cut(s)	361, 1146
AvaII	GGWCC	1 cut(s)	770
BaeGI	GKGCMC	1 cut(s)	899
BanI	GGYRCC	4 cut(s)	536, 592, 1117, 1989
BanII	GRGCYC	1 cut(s)	899
BbsI	GAAGAC	2 cut(s)	221, 1688
Bbv12I	GWGCWC	1 cut(s)	1913
BbvCI	CCTCAGC	1 cut(s)	582
BbvI	GCAGC	5 cut(s)	407, 930, 1033, 1480, 1523
BccI	CCATC	1 cut(s)	965
BceAI	ACGGC	2 cut(s)	254, 2188
BcgI	CGANNNNNNTGC	4 cut(s)	341, 375, 1475, 1509
BciT130I	CCWGG	4 cut(s)	900, 1082, 1275, 1479
BciVI	GTATCC	2 cut(s)	833, 2159
BclI	TGATCA	2 cut(s)	1885, 2035
BcnI	CCSGG	1 cut(s)	669
BcoDI	GTCTC	1 cut(s)	822
BfaI	CTAG	2 cut(s)	276, 1923
BfrI	CTTAAG	2 cut(s)	1285, 1520
BfuI	GTATCC	2 cut(s)	833, 2159
BglI	GCCNNNNNGGC	1 cut(s)	586
BisI	GCNGC	6 cut(s)	396, 587, 944, 1022, 1494, 1512
BlsI	GCNGC	6 cut(s)	397, 588, 945, 1023, 1495, 1513
BmcAI	AGTACT	1 cut(s)	455
Bme1390I	CCNGG	5 cut(s)	669, 900, 1082, 1275, 1479
Bme18I	GGWCC	1 cut(s)	770
BmgT120I	GGNCC	5 cut(s)	578, 770, 895, 896, 1312
BmiI	GGNNCC	9 cut(s)	538, 594, 680, 896, 897, 1119, 1483, 1670, 1991
BmrFI	CCNGG	5 cut(s)	669, 900, 1082, 1275, 1479
BmsI	GCATC	8 cut(s)	339, 475, 481, 930, 1171, 1299, 1379, 1739
BpiI	GAAGAC	2 cut(s)	221, 1688
BpmI	CTGGAG	4 cut(s)	57, 968, 1069, 1103
Bpu10I	CCTNAGC	1 cut(s)	582
Bpu14I	TTCGAA	2 cut(s)	361, 1146
BpuMI	CCSGG	1 cut(s)	669
BsaAI	YACGTR	1 cut(s)	420
BsaHI	GRCGYC	2 cut(s)	165, 996
BsaJI	CCNNGG	5 cut(s)	884, 898, 1274, 1477, 2170
BsaWI	WCCGGW	1 cut(s)	227
BsaXI	ACNNNNNCTCC	4 cut(s)	819, 849, 2119, 2149
Bse1I	ACTGG	5 cut(s)	1360, 1642, 1708, 1880, 2167
BseBI	CCWGG	4 cut(s)	900, 1082, 1275, 1479
BseDI	CCNNGG	5 cut(s)	884, 898, 1274, 1477, 2170
BseGI	GGATG	4 cut(s)	945, 1162, 1370, 1888
BseMII	CTCAG	2 cut(s)	596, 720
BseNI	ACTGG	5 cut(s)	1360, 1642, 1708, 1880, 2167
BseRI	GAGGAG	2 cut(s)	344, 1433
BseSI	GKGCMC	1 cut(s)	899
BseXI	GCAGC	5 cut(s)	407, 930, 1033, 1480, 1523
BsgI	GTGCAG	2 cut(s)	226, 1530
Bsh1236I	CGCG	1 cut(s)	284
BshFI	GGCC	5 cut(s)	269, 580, 897, 1313, 2175
BshNI	GGYRCC	4 cut(s)	536, 592, 1117, 1989
BsiHKAI	GWGCWC	1 cut(s)	1913
BsiSI	CCGG	2 cut(s)	228, 668
BslFI	GGGAC	3 cut(s)	257, 1189, 1866
BsmAI	GTCTC	1 cut(s)	822
BsmFI	GGGAC	3 cut(s)	257, 1189, 1866
BsnI	GGCC	5 cut(s)	269, 580, 897, 1313, 2175
Bsp119I	TTCGAA	2 cut(s)	361, 1146
Bsp120I	GGGCCC	1 cut(s)	895
Bsp1286I	GDGCHC	2 cut(s)	899, 1913
BspACI	CCGC	5 cut(s)	311, 393, 586, 589, 661
BspANI	GGCC	5 cut(s)	269, 580, 897, 1313, 2175
BspCNI	CTCAG	2 cut(s)	595, 719
BspFNI	CGCG	1 cut(s)	284
BspLI	GGNNCC	9 cut(s)	538, 594, 680, 896, 897, 1119, 1483, 1670, 1991
BspPI	GGATC	5 cut(s)	34, 175, 751, 1603, 1856
BspQI	GCTCTTC	1 cut(s)	1042
BspT104I	TTCGAA	2 cut(s)	361, 1146
BspT107I	GGYRCC	4 cut(s)	536, 592, 1117, 1989
BspTI	CTTAAG	2 cut(s)	1285, 1520
BsrI	ACTGG	5 cut(s)	1360, 1642, 1708, 1880, 2167
BssECI	CCNNGG	5 cut(s)	884, 898, 1274, 1477, 2170
BssNI	GRCGYC	2 cut(s)	165, 996
Bst2UI	CCWGG	4 cut(s)	900, 1082, 1275, 1479
Bst4CI	ACNGT	5 cut(s)	382, 1100, 1582, 1761, 1819
Bst6I	CTCTTC	2 cut(s)	444, 1042
BstACI	GRCGYC	2 cut(s)	165, 996
BstAFI	CTTAAG	2 cut(s)	1285, 1520
BstAPI	GCANNNNNTGC	1 cut(s)	401
BstBAI	YACGTR	1 cut(s)	420
BstBI	TTCGAA	2 cut(s)	361, 1146
BstC8I	GCNNGC	2 cut(s)	813, 1058
BstDEI	CTNAG	4 cut(s)	246, 582, 706, 1235
BstDSI	CCRYGG	1 cut(s)	2170
BstENI	CCTNNNNNAGG	1 cut(s)	1415
BstF5I	GGATG	4 cut(s)	945, 1162, 1370, 1888
BstFNI	CGCG	1 cut(s)	284
BstHHI	GCGC	1 cut(s)	284
BstMAI	GTCTC	1 cut(s)	822
BstMWI	GCNNNNNNNGC	3 cut(s)	401, 586, 1834
BstNI	CCWGG	4 cut(s)	900, 1082, 1275, 1479
BstNSI	RCATGY	1 cut(s)	1955
BstSCI	CCNGG	5 cut(s)	667, 898, 1080, 1273, 1477
BstSLI	GKGCMC	1 cut(s)	899
BstSNI	TACGTA	1 cut(s)	420
BstUI	CGCG	1 cut(s)	284
BstV1I	GCAGC	5 cut(s)	407, 930, 1033, 1480, 1523
BstV2I	GAAGAC	2 cut(s)	221, 1688
BstX2I	RGATCY	3 cut(s)	26, 180, 743
BstXI	CCANNNNNNTGG	3 cut(s)	120, 689, 2000
BstYI	RGATCY	3 cut(s)	26, 180, 743
BsuI	GTATCC	2 cut(s)	833, 2159
BsuRI	GGCC	5 cut(s)	269, 580, 897, 1313, 2175
BtgI	CCRYGG	1 cut(s)	2170
BtsCI	GGATG	4 cut(s)	945, 1162, 1370, 1888
BtsIMutI	CAGTG	3 cut(s)	714, 1701, 2160
Cac8I	GCNNGC	2 cut(s)	813, 1058
CfoI	GCGC	1 cut(s)	284
Cfr13I	GGNCC	5 cut(s)	578, 770, 895, 896, 1312
CseI	GACGC	1 cut(s)	985
Csp6I	GTAC	6 cut(s)	409, 421, 454, 537, 593, 1762
CviAII	CATG	8 cut(s)	265, 683, 1673, 1952, 1958, 1973, 1979, 2090
CviQI	GTAC	6 cut(s)	409, 421, 454, 537, 593, 1762
DdeI	CTNAG	4 cut(s)	246, 582, 706, 1235
EaeI	YGGCCR	2 cut(s)	267, 2173
Eam1104I	CTCTTC	2 cut(s)	444, 1042
EarI	CTCTTC	2 cut(s)	444, 1042
Eco105I	TACGTA	1 cut(s)	420
Eco24I	GRGCYC	1 cut(s)	899
Eco47I	GGWCC	1 cut(s)	770
Eco57I	CTGAAG	2 cut(s)	468, 1729
EcoNI	CCTNNNNNAGG	1 cut(s)	1415
EcoO109I	RGGNCCY	3 cut(s)	770, 895, 1312
EcoRI	GAATTC	1 cut(s)	334
EcoRII	CCWGG	4 cut(s)	898, 1080, 1273, 1477
EcoT38I	GRGCYC	1 cut(s)	899
FaeI	CATG	8 cut(s)	268, 686, 1676, 1955, 1961, 1976, 1982, 2093
FaqI	GGGAC	3 cut(s)	257, 1189, 1866
FatI	CATG	8 cut(s)	264, 682, 1672, 1951, 1957, 1972, 1978, 2089
FauI	CCCGC	1 cut(s)	582
FauNDI	CATATG	1 cut(s)	1766
FbaI	TGATCA	2 cut(s)	1885, 2035
FblI	GTMKAC	3 cut(s)	1260, 1577, 1795
Fnu4HI	GCNGC	6 cut(s)	396, 587, 944, 1022, 1494, 1512
FokI	GGATG	4 cut(s)	952, 1149, 1357, 1895
FriOI	GRGCYC	1 cut(s)	899
Fsp4HI	GCNGC	6 cut(s)	396, 587, 944, 1022, 1494, 1512
FspBI	CTAG	2 cut(s)	276, 1923
GlaI	GCGC	1 cut(s)	283
GluI	GCNGC	6 cut(s)	396, 587, 944, 1022, 1494, 1512
GsuI	CTGGAG	4 cut(s)	57, 968, 1069, 1103
HaeIII	GGCC	5 cut(s)	269, 580, 897, 1313, 2175
HapII	CCGG	2 cut(s)	228, 668
HgaI	GACGC	1 cut(s)	985
HhaI	GCGC	1 cut(s)	284
Hin1I	GRCGYC	2 cut(s)	165, 996
Hin1II	CATG	8 cut(s)	268, 686, 1676, 1955, 1961, 1976, 1982, 2093
Hin6I	GCGC	1 cut(s)	282
HinP1I	GCGC	1 cut(s)	282
HincII	GTYRAC	3 cut(s)	905, 1000, 1455
HindII	GTYRAC	3 cut(s)	905, 1000, 1455
HindIII	AAGCTT	1 cut(s)	1660
HinfI	GANTC	9 cut(s)	174, 224, 1177, 1197, 1270, 1526, 1649, 1734, 1780
HpaI	GTTAAC	1 cut(s)	1455
HpaII	CCGG	2 cut(s)	228, 668
HphI	GGTGA	8 cut(s)	121, 242, 305, 683, 1032, 1370, 1399, 2078
Hpy166II	GTNNAC	7 cut(s)	233, 905, 1000, 1261, 1455, 1578, 1796
Hpy188I	TCNGA	9 cut(s)	26, 223, 698, 757, 885, 1748, 1785, 1848, 1908
Hpy188III	TCNNGA	8 cut(s)	36, 178, 376, 505, 773, 1048, 1225, 1731
Hpy8I	GTNNAC	7 cut(s)	233, 905, 1000, 1261, 1455, 1578, 1796
Hpy99I	CGWCG	2 cut(s)	167, 170
HpyAV	CCTTC	8 cut(s)	606, 864, 915, 1130, 1134, 1843, 2093, 2118
HpyCH4III	ACNGT	5 cut(s)	382, 1100, 1582, 1761, 1819
HpyCH4IV	ACGT	5 cut(s)	165, 419, 1248, 1385, 1574
HpyF10VI	GCNNNNNNNGC	3 cut(s)	401, 586, 1834
HpyF3I	CTNAG	4 cut(s)	246, 582, 706, 1235
HpySE526I	ACGT	5 cut(s)	165, 419, 1248, 1385, 1574
Hsp92I	GRCGYC	2 cut(s)	165, 996
Hsp92II	CATG	8 cut(s)	268, 686, 1676, 1955, 1961, 1976, 1982, 2093
HspAI	GCGC	1 cut(s)	282
KpnI	GGTACC	2 cut(s)	540, 596
Ksp22I	TGATCA	2 cut(s)	1885, 2035
KspAI	GTTAAC	1 cut(s)	1455
LguI	GCTCTTC	1 cut(s)	1042
LmnI	GCTCC	1 cut(s)	1420
Lsp1109I	GCAGC	5 cut(s)	407, 930, 1033, 1480, 1523
LweI	GCATC	8 cut(s)	339, 475, 481, 930, 1171, 1299, 1379, 1739
MaeI	CTAG	2 cut(s)	276, 1923
MaeII	ACGT	5 cut(s)	165, 419, 1248, 1385, 1574
MaeIII	GTNAC	4 cut(s)	43, 519, 1198, 1405
MflI	RGATCY	3 cut(s)	26, 180, 743
MhlI	GDGCHC	2 cut(s)	899, 1913
MlyI	GAGTC	6 cut(s)	183, 1171, 1206, 1279, 1520, 1643
MmeI	TCCRAC	4 cut(s)	151, 817, 1757, 1838
MroXI	GAANNNNTTC	1 cut(s)	2128
MslI	CAYNNNNRTG	7 cut(s)	1167, 1412, 1769, 1950, 1956, 1977, 1998
MspA1I	CMGCKG	1 cut(s)	586
MspCI	CTTAAG	2 cut(s)	1285, 1520
MspI	CCGG	2 cut(s)	228, 668
MspR9I	CCNGG	5 cut(s)	669, 900, 1082, 1275, 1479
MvaI	CCWGG	4 cut(s)	900, 1082, 1275, 1479
MvnI	CGCG	1 cut(s)	284
MwoI	GCNNNNNNNGC	3 cut(s)	401, 586, 1834
NciI	CCSGG	1 cut(s)	669
NdeI	CATATG	1 cut(s)	1766
NlaIII	CATG	8 cut(s)	268, 686, 1676, 1955, 1961, 1976, 1982, 2093
NlaIV	GGNNCC	9 cut(s)	538, 594, 680, 896, 897, 1119, 1483, 1670, 1991
NmeAIII	GCCGAG	1 cut(s)	1579
NmuCI	GTSAC	2 cut(s)	1198, 1405
NspI	RCATGY	1 cut(s)	1955
NspV	TTCGAA	2 cut(s)	361, 1146
PciI	ACATGT	1 cut(s)	1951
PciSI	GCTCTTC	1 cut(s)	1042
PcsI	WCGNNNNNNNCGW	1 cut(s)	207
PdmI	GAANNNNTTC	1 cut(s)	2128
PfeI	GAWTC	3 cut(s)	224, 1734, 1780
PflMI	CCANNNNNTGG	2 cut(s)	916, 1127
PfoI	TCCNGGA	1 cut(s)	1080
PkrI	GCNGC	6 cut(s)	397, 588, 945, 1023, 1495, 1513
PleI	GAGTC	6 cut(s)	182, 1171, 1205, 1278, 1520, 1643
PpsI	GAGTC	6 cut(s)	182, 1171, 1205, 1278, 1520, 1643
Ppu21I	YACGTR	1 cut(s)	420
PpuMI	RGGWCCY	1 cut(s)	770
PscI	ACATGT	1 cut(s)	1951
PshBI	ATTAAT	2 cut(s)	1212, 1935
PsiI	TTATAA	1 cut(s)	1536
Psp1406I	AACGTT	1 cut(s)	1385
Psp5II	RGGWCCY	1 cut(s)	770
Psp6I	CCWGG	4 cut(s)	898, 1080, 1273, 1477
PspGI	CCWGG	4 cut(s)	898, 1080, 1273, 1477
PspN4I	GGNNCC	9 cut(s)	538, 594, 680, 896, 897, 1119, 1483, 1670, 1991
PspOMI	GGGCCC	1 cut(s)	895
PspPI	GGNCC	5 cut(s)	578, 770, 895, 896, 1312
PspPPI	RGGWCCY	1 cut(s)	770
PsrI	GAACNNNNNNTAC	2 cut(s)	2150, 2182
PsuI	RGATCY	3 cut(s)	26, 180, 743
RsaI	GTAC	6 cut(s)	410, 422, 455, 538, 594, 1763
RsaNI	GTAC	6 cut(s)	409, 421, 454, 537, 593, 1762
RseI	CAYNNNNRTG	7 cut(s)	1167, 1412, 1769, 1950, 1956, 1977, 1998
SapI	GCTCTTC	1 cut(s)	1042
SatI	GCNGC	6 cut(s)	396, 587, 944, 1022, 1494, 1512
Sau96I	GGNCC	5 cut(s)	578, 770, 895, 896, 1312
ScaI	AGTACT	1 cut(s)	455
SchI	GAGTC	6 cut(s)	183, 1171, 1206, 1279, 1520, 1643
ScrFI	CCNGG	5 cut(s)	669, 900, 1082, 1275, 1479
SduI	GDGCHC	2 cut(s)	899, 1913
SfaNI	GCATC	8 cut(s)	339, 475, 481, 930, 1171, 1299, 1379, 1739
SfuI	TTCGAA	2 cut(s)	361, 1146
SinI	GGWCC	1 cut(s)	770
SmiMI	CAYNNNNRTG	7 cut(s)	1167, 1412, 1769, 1950, 1956, 1977, 1998
SmlI	CTYRAG	2 cut(s)	1285, 1520
SmoI	CTYRAG	2 cut(s)	1285, 1520
SnaBI	TACGTA	1 cut(s)	420
SsiI	CCGC	5 cut(s)	311, 393, 586, 589, 661
SspI	AATATT	1 cut(s)	850
SspMI	CTAG	2 cut(s)	276, 1923
StyD4I	CCNGG	5 cut(s)	667, 898, 1080, 1273, 1477
TaaI	ACNGT	5 cut(s)	382, 1100, 1582, 1761, 1819
TaiI	ACGT	5 cut(s)	168, 422, 1251, 1388, 1577
TaqI	TCGA	9 cut(s)	194, 201, 302, 321, 361, 861, 1104, 1146, 1459
TatI	WGTACW	2 cut(s)	453, 1761
TauI	GCSGC	1 cut(s)	589
TfiI	GAWTC	3 cut(s)	224, 1734, 1780
TscAI	CASTG	3 cut(s)	714, 1708, 2167
TseFI	GTSAC	2 cut(s)	1198, 1405
TseI	GCWGC	5 cut(s)	395, 943, 1021, 1493, 1511
Tsp45I	GTSAC	2 cut(s)	1198, 1405
TspRI	CASTG	3 cut(s)	714, 1708, 2167
Van91I	CCANNNNNTGG	2 cut(s)	916, 1127
Vha464I	CTTAAG	2 cut(s)	1285, 1520
VpaK11BI	GGWCC	1 cut(s)	770
VspI	ATTAAT	2 cut(s)	1212, 1935
XagI	CCTNNNNNAGG	1 cut(s)	1415
XapI	RAATTY	4 cut(s)	4, 334, 913, 1075
XceI	RCATGY	1 cut(s)	1955
XcmI	CCANNNNNNNNNTGG	3 cut(s)	117, 1271, 1970
XmiI	GTMKAC	3 cut(s)	1260, 1577, 1795
XmnI	GAANNNNTTC	1 cut(s)	2128
XspI	CTAG	2 cut(s)	276, 1923
ZraI	GACGTC	1 cut(s)	166
ZrmI	AGTACT	1 cut(s)	455

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.