FvH4_5g02430

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003273
Physical Location & Seq
Reverse (-)
1482147 .. 1484202
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02430.t1

Sequence Viewer

Length: 930 bp
ATGGATATTACCTATATATATAAGCTCTTGGCACGTTACGAATATGTTGCAAAAACAAAGTCGCACTTTTTGCTGATAATGATGATGAATCATAGCAAGTCCATATCCACTTCTTTGTTTCTGCTGCTGCTGCTTCTTCAAATTTTGCACCCAACACCTATTACAGCTCAAGAAGTTGAGGACGAAACGGAATTTGATTATCTGAAAGATAGTGGGAAAGGACCAGAGCATTGGGGAGATCTTAGGGAAGACTGGGCAGCATGTAAAAATGGGACAATGCAATCTCCAATAGATTTGCGTAGTGAGATCGTGCAAGTACGCCCCAACAAAGGAGAGGTGAAGCTCAAGTACAAGCCTTCAAATGCAACAATCAGGAATAGGGGCCATGACATTTCGATCCAGTGGAAGCTAGGTGATGCTGGATCCGTGGAGATTAATGGCACAGATTACTGGCTAATTCAAGGCCACTGGCACTCACCATCTGAGCATACAATTAACGGTAAAAGATTTGACCTGGAGCTTCATATGGTTCACCTCATTCCTGATGCAAATGTCGAAAACAACATAGCTGTGTTAGCATACCTCTACAAAATTGGCAAGCCTGACAAGTTTCTCTCCGAGGTAAGCAAAGATATCAAGAGCTTGATTAATGTAAAGGAAGAGAGACACATAGGGGTGGTTGATCCAAGAAATGTGAGGAAGGGTAGCGCAAATTTTTACAAGTACAAAGGCTCACTCACTGTACCTCCTTGCACTGAAGGAGTAACTTGGATCGTAGACAAAGAGTTAACTACTGTATCAAGAGAGCAAGTGGAGTTGCTTCGAGAAGCCGTGGATGACTCCGCAGAAACGAATGCAAGACCATTACAAGCTCTGAATGGTCGAAAAGTGCAGTACTTCTATGGCCATGAGTCTCACATAAGGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

310

Amino Acids

35.38

Weight (kDa)

6.51

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 73 - 299 2.3e-44 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 777
AciI CCGC 1 cut(s) 843
AclWI GGATC 5 cut(s) 391, 417, 430, 677, 779
AcoI YGGCCR 1 cut(s) 904
AcsI RAATTY 3 cut(s) 141, 191, 712
AcuI CTGAAG 1 cut(s) 777
AfaI GTAC 5 cut(s) 318, 350, 725, 744, 896
AfiI CCNNNNNNNGG 1 cut(s) 329
AgsI TTSAA 3 cut(s) 140, 360, 461
AjnI CCWGG 1 cut(s) 513
AluBI AGCT 8 cut(s) 25, 167, 343, 409, 520, 569, 642, 872
AluI AGCT 8 cut(s) 25, 167, 343, 409, 520, 569, 642, 872
Alw26I GTCTC 2 cut(s) 658, 918
AlwI GGATC 5 cut(s) 391, 417, 430, 677, 779
AoxI GGCC 3 cut(s) 382, 463, 904
ApeKI GCWGC 4 cut(s) 124, 127, 130, 257
ApoI RAATTY 3 cut(s) 141, 191, 712
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AseI ATTAAT 2 cut(s) 435, 648
AspLEI GCGC 1 cut(s) 710
AspS9I GGNCC 2 cut(s) 221, 382
AsuHPI GGTGA 4 cut(s) 349, 425, 468, 524
AvaII GGWCC 1 cut(s) 221
BaeI ACNNNNGTAYC 2 cut(s) 780, 813
BalI TGGCCA 1 cut(s) 906
BamHI GGATCC 1 cut(s) 422
BarI GAAGNNNNNNTAC 2 cut(s) 332, 364
BbsI GAAGAC 1 cut(s) 255
BbvI GCAGC 4 cut(s) 111, 114, 117, 269
BccI CCATC 1 cut(s) 487
BceAI ACGGC 1 cut(s) 815
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 1 cut(s) 515
BcoDI GTCTC 2 cut(s) 658, 918
BfaI CTAG 1 cut(s) 410
BglII AGATCT 1 cut(s) 238
BisI GCNGC 4 cut(s) 125, 128, 131, 258
BlsI GCNGC 4 cut(s) 126, 129, 132, 259
BmcAI AGTACT 1 cut(s) 896
Bme1390I CCNGG 1 cut(s) 515
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 2 cut(s) 221, 382
BmiI GGNNCC 2 cut(s) 383, 424
BmrFI CCNGG 1 cut(s) 515
BmrI ACTGGG 1 cut(s) 262
BmsI GCATC 2 cut(s) 406, 535
BmuI ACTGGG 1 cut(s) 262
BpiI GAAGAC 1 cut(s) 255
BpmI CTGGAG 1 cut(s) 536
BpuEI CTTGAG 2 cut(s) 153, 329
BsaJI CCNNGG 3 cut(s) 426, 618, 831
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bsc4I CCNNNNNNNGG 1 cut(s) 329
Bse1I ACTGG 4 cut(s) 257, 400, 455, 473
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 3 cut(s) 426, 618, 831
BseGI GGATG 1 cut(s) 841
BseLI CCNNNNNNNGG 1 cut(s) 329
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 4 cut(s) 257, 400, 455, 473
BseXI GCAGC 4 cut(s) 111, 114, 117, 269
BsgI GTGCAG 1 cut(s) 911
BshFI GGCC 3 cut(s) 384, 465, 906
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 329
BsmAI GTCTC 2 cut(s) 658, 918
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 3 cut(s) 384, 465, 906
Bsp143I GATC 6 cut(s) 238, 306, 396, 422, 682, 771
BspACI CCGC 1 cut(s) 843
BspANI GGCC 3 cut(s) 384, 465, 906
BspCNI CTCAG 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 383, 424
BspPI GGATC 5 cut(s) 391, 417, 430, 677, 779
BsrI ACTGG 4 cut(s) 257, 400, 455, 473
BssECI CCNNGG 3 cut(s) 426, 618, 831
BssMI GATC 6 cut(s) 238, 306, 396, 422, 682, 771
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 3 cut(s) 500, 742, 796
Bst6I CTCTTC 1 cut(s) 654
BstAPI GCANNNNNTGC 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 599
BstDEI CTNAG 2 cut(s) 242, 483
BstDSI CCRYGG 2 cut(s) 426, 831
BstF5I GGATG 1 cut(s) 841
BstHHI GCGC 1 cut(s) 710
BstKTI GATC 6 cut(s) 241, 309, 399, 425, 685, 774
BstMAI GTCTC 2 cut(s) 658, 918
BstMBI GATC 6 cut(s) 238, 306, 396, 422, 682, 771
BstMWI GCNNNNNNNGC 3 cut(s) 70, 130, 575
BstNI CCWGG 1 cut(s) 515
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 111, 114, 117, 269
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 2 cut(s) 238, 422
BstXI CCANNNNNNTGG 1 cut(s) 231
BstYI RGATCY 2 cut(s) 238, 422
BsuRI GGCC 3 cut(s) 384, 465, 906
BtgI CCRYGG 2 cut(s) 426, 831
BtsCI GGATG 1 cut(s) 841
BtsIMutI CAGTG 4 cut(s) 407, 466, 738, 753
Cac8I GCNNGC 1 cut(s) 599
CfoI GCGC 1 cut(s) 710
Cfr13I GGNCC 2 cut(s) 221, 382
Csp6I GTAC 5 cut(s) 317, 349, 724, 743, 895
CviAII CATG 3 cut(s) 261, 386, 908
CviQI GTAC 5 cut(s) 317, 349, 724, 743, 895
DdeI CTNAG 2 cut(s) 242, 483
DpnI GATC 6 cut(s) 240, 308, 398, 424, 684, 773
DpnII GATC 6 cut(s) 238, 306, 396, 422, 682, 771
EaeI YGGCCR 1 cut(s) 904
Eam1104I CTCTTC 1 cut(s) 654
EarI CTCTTC 1 cut(s) 654
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 777
EcoRII CCWGG 1 cut(s) 513
EcoRV GATATC 1 cut(s) 634
FaeI CATG 3 cut(s) 264, 389, 911
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 1 cut(s) 286
FatI CATG 3 cut(s) 260, 385, 907
FauNDI CATATG 1 cut(s) 525
FblI GTMKAC 1 cut(s) 777
Fnu4HI GCNGC 4 cut(s) 125, 128, 131, 258
FokI GGATG 1 cut(s) 848
Fsp4HI GCNGC 4 cut(s) 125, 128, 131, 258
FspBI CTAG 1 cut(s) 410
GlaI GCGC 1 cut(s) 709
GluI GCNGC 4 cut(s) 125, 128, 131, 258
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 3 cut(s) 384, 465, 906
HhaI GCGC 1 cut(s) 710
Hin1II CATG 3 cut(s) 264, 389, 911
Hin6I GCGC 1 cut(s) 708
HinP1I GCGC 1 cut(s) 708
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HinfI GANTC 3 cut(s) 88, 839, 911
HpaI GTTAAC 1 cut(s) 789
HphI GGTGA 4 cut(s) 349, 425, 468, 524
Hpy166II GTNNAC 3 cut(s) 532, 778, 789
Hpy188I TCNGA 4 cut(s) 204, 484, 619, 876
Hpy188III TCNNGA 6 cut(s) 170, 373, 542, 637, 801, 824
Hpy8I GTNNAC 3 cut(s) 532, 778, 789
HpyAV CCTTC 3 cut(s) 366, 694, 752
HpyCH4III ACNGT 3 cut(s) 500, 742, 796
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 9 cut(s) 50, 148, 280, 313, 365, 548, 753, 857, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 130, 575
HpyF3I CTNAG 2 cut(s) 242, 483
HpySE526I ACGT 1 cut(s) 34
Hsp92II CATG 3 cut(s) 264, 389, 911
HspAI GCGC 1 cut(s) 708
KspAI GTTAAC 1 cut(s) 789
Kzo9I GATC 6 cut(s) 238, 306, 396, 422, 682, 771
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 4 cut(s) 111, 114, 117, 269
LweI GCATC 2 cut(s) 406, 535
MaeI CTAG 1 cut(s) 410
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 2 cut(s) 35, 763
MalI GATC 6 cut(s) 240, 308, 398, 424, 684, 773
MboI GATC 6 cut(s) 238, 306, 396, 422, 682, 771
MboII GAAGA 3 cut(s) 128, 260, 671
MflI RGATCY 2 cut(s) 238, 422
MlsI TGGCCA 1 cut(s) 906
MluCI AATT 6 cut(s) 141, 191, 456, 492, 591, 712
MluNI TGGCCA 1 cut(s) 906
MlyI GAGTC 2 cut(s) 833, 920
MnlI CCTC 7 cut(s) 172, 328, 545, 593, 613, 690, 756
Mox20I TGGCCA 1 cut(s) 906
MscI TGGCCA 1 cut(s) 906
MseI TTAA 5 cut(s) 435, 495, 648, 788, 928
MslI CAYNNNNRTG 2 cut(s) 569, 674
Msp20I TGGCCA 1 cut(s) 906
MspR9I CCNGG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 1 cut(s) 515
MwoI GCNNNNNNNGC 3 cut(s) 70, 130, 575
NdeI CATATG 1 cut(s) 525
NdeII GATC 6 cut(s) 238, 306, 396, 422, 682, 771
NlaIII CATG 3 cut(s) 264, 389, 911
NlaIV GGNNCC 2 cut(s) 383, 424
NspI RCATGY 1 cut(s) 264
PctI GAATGC 1 cut(s) 859
PfeI GAWTC 1 cut(s) 88
PkrI GCNGC 4 cut(s) 126, 129, 132, 259
PleI GAGTC 2 cut(s) 833, 919
PpsI GAGTC 2 cut(s) 833, 919
PshBI ATTAAT 2 cut(s) 435, 648
Psp6I CCWGG 1 cut(s) 513
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 2 cut(s) 383, 424
PspPI GGNCC 2 cut(s) 221, 382
PsuI RGATCY 2 cut(s) 238, 422
RsaI GTAC 5 cut(s) 318, 350, 725, 744, 896
RsaNI GTAC 5 cut(s) 317, 349, 724, 743, 895
RseI CAYNNNNRTG 2 cut(s) 569, 674
SaqAI TTAA 5 cut(s) 435, 495, 648, 788, 928
SatI GCNGC 4 cut(s) 125, 128, 131, 258
Sau3AI GATC 6 cut(s) 238, 306, 396, 422, 682, 771
Sau96I GGNCC 2 cut(s) 221, 382
ScaI AGTACT 1 cut(s) 896
SchI GAGTC 2 cut(s) 833, 920
ScrFI CCNGG 1 cut(s) 515
SfaNI GCATC 2 cut(s) 406, 535
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 569, 674
SmlI CTYRAG 2 cut(s) 168, 344
SmoI CTYRAG 2 cut(s) 168, 344
Sse9I AATT 6 cut(s) 141, 191, 456, 492, 591, 712
SsiI CCGC 1 cut(s) 843
SspMI CTAG 1 cut(s) 410
StyD4I CCNGG 1 cut(s) 513
TaaI ACNGT 3 cut(s) 500, 742, 796
TaiI ACGT 1 cut(s) 37
TaqI TCGA 4 cut(s) 395, 555, 823, 883
TasI AATT 6 cut(s) 141, 191, 456, 492, 591, 712
TatI WGTACW 3 cut(s) 348, 723, 894
TfiI GAWTC 1 cut(s) 88
Tru1I TTAA 5 cut(s) 435, 495, 648, 788, 928
Tru9I TTAA 5 cut(s) 435, 495, 648, 788, 928
TscAI CASTG 4 cut(s) 407, 473, 745, 760
TseI GCWGC 4 cut(s) 124, 127, 130, 257
TspDTI ATGAA 2 cut(s) 101, 512
TspGWI ACGGA 2 cut(s) 203, 415
TspRI CASTG 4 cut(s) 407, 473, 745, 760
VpaK11BI GGWCC 1 cut(s) 221
VspI ATTAAT 2 cut(s) 435, 648
XapI RAATTY 3 cut(s) 141, 191, 712
XceI RCATGY 1 cut(s) 264
XmiI GTMKAC 1 cut(s) 777
XspI CTAG 1 cut(s) 410
ZrmI AGTACT 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.