FvH4_6g16680

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003273
Physical Location & Seq
Forward (+)
10573461 .. 10575766
2306 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16680.t1

Sequence Viewer

Length: 963 bp
ATGAAGCATCAAAGCAAACCCACCTTCATTTCATTTTCTGTATCATTTGTACTTCTCTTCTCACATTGGATACAAGTCACAGTTGGTCTTGTAGTATCAATAAAACCGCAGCCCATAACCAAACGTTTAGGTCCAATCCGTGTGAATATCCTGCCTCCATCCTTGGTCAAAGCTCAAGAAGTTGGGGATGAGAGAGATTTTGATTATGTTGAGGGAAGTAAGAAGGGGCCAGCTCAATGGGGAGAGATCAAGAACGAATGGGCCGCATGGAAGAGTGGGGGCTTGCAATCTCCCATAGATTTATCAGATCAGAGAGTTCAAATAGTCCCAAACTTGGGAGAGCTAAAGATCAATCACAAGCCTTCTAACGCCACTCTCAAGAATAGGGGTCATGATATCAAGCTTCGATGGGTTGGCGACGCTGGATTGATTGAAATCGACAGTATTGAGTATTTTTTGCAACAAGCTCACTGGCACTCGCCCTCGGATCATCTCATCAATGGCAAAAGGTACGATATGGAGCTCCACATGGTTTATGCAAGCCCGGATCCGAAAGTCCAAAACAGTATTGTTGTTGTTGGAGTCCTTTACGAGATTGGTCGTCCAGACTCTTTCCTCTCTAAGTTGATGAAGGACGTAGCGTTGATGACAAATCAAAAGGCAGAGAGAAGCATAGGAGTGATTGATCCTGCACAAATAATCCAACTGGGCGGCAACAAAAACTACTACAGATATGTGGGATCTCTCACCATCCCTCCATGTACTGAAGGCGTTATTTGGATCATCAGCAAACAGTTTGGAACGGTGTCAAAGGTACAAATGAATCTACTTCGCGTGGCTGTGCATGATGCGGTGTCGATTCTTCGGTGCGGGTCGGTCTTTCGTCATCGCTACGGGCATGACGTCACGGGGGCGTATGAGGGTATGATGTCATGGTCCTGCTTCTATATGCCCAAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

321

Amino Acids

36.06

Weight (kDa)

9.15

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 76 - 281 3.5e-35 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 906
AccII CGCG 1 cut(s) 834
AciI CCGC 5 cut(s) 107, 264, 711, 851, 870
AclI AACGTT 1 cut(s) 124
AclWI GGATC 6 cut(s) 495, 542, 555, 680, 748, 788
AcuI CTGAAG 1 cut(s) 786
AcyI GRCGYC 1 cut(s) 903
AfaI GTAC 4 cut(s) 51, 512, 763, 816
AfiI CCNNNNNNNGG 2 cut(s) 334, 335
AgsI TTSAA 2 cut(s) 320, 434
AluBI AGCT 6 cut(s) 173, 233, 343, 403, 467, 523
AluI AGCT 6 cut(s) 173, 233, 343, 403, 467, 523
Alw21I GWGCWC 1 cut(s) 525
AlwI GGATC 6 cut(s) 495, 542, 555, 680, 748, 788
AoxI GGCC 2 cut(s) 227, 261
ApeKI GCWGC 1 cut(s) 109
AspS9I GGNCC 4 cut(s) 131, 227, 261, 936
AsuC2I CCSGG 1 cut(s) 545
AsuHPI GGTGA 1 cut(s) 739
AvaII GGWCC 2 cut(s) 131, 936
BamHI GGATCC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 121
BccI CCATC 3 cut(s) 166, 402, 758
BciVI GTATCC 1 cut(s) 63
BcnI CCSGG 1 cut(s) 545
BfmI CTRYAG 1 cut(s) 727
BfuI GTATCC 1 cut(s) 63
BisI GCNGC 3 cut(s) 110, 264, 712
BlsI GCNGC 3 cut(s) 111, 265, 713
Bme1390I CCNGG 1 cut(s) 545
Bme18I GGWCC 2 cut(s) 131, 936
BmgT120I GGNCC 4 cut(s) 131, 227, 261, 936
BmiI GGNNCC 2 cut(s) 228, 549
BmrFI CCNGG 1 cut(s) 545
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 2 cut(s) 16, 838
BmuI ACTGGG 1 cut(s) 716
BpuEI CTTGAG 2 cut(s) 159, 362
BpuMI CCSGG 1 cut(s) 545
BsaBI GATNNNNATC 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 903
BsaJI CCNNGG 2 cut(s) 162, 483
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 335
Bse1I ACTGG 2 cut(s) 476, 711
Bse8I GATNNNNATC 1 cut(s) 434
BseDI CCNNGG 2 cut(s) 162, 483
BseGI GGATG 3 cut(s) 158, 193, 750
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 2 cut(s) 334, 335
BseNI ACTGG 2 cut(s) 476, 711
BseXI GCAGC 1 cut(s) 121
BsgI GTGCAG 1 cut(s) 675
Bsh1236I CGCG 1 cut(s) 834
BshFI GGCC 2 cut(s) 229, 263
BsiHKAI GWGCWC 1 cut(s) 525
BsiSI CCGG 1 cut(s) 545
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 334, 335
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 2 cut(s) 229, 263
Bsp1286I GDGCHC 1 cut(s) 525
Bsp143I GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
BspACI CCGC 5 cut(s) 107, 264, 711, 851, 870
BspANI GGCC 2 cut(s) 229, 263
BspFNI CGCG 1 cut(s) 834
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 228, 549
BspPI GGATC 6 cut(s) 495, 542, 555, 680, 748, 788
BsrI ACTGG 2 cut(s) 476, 711
BssECI CCNNGG 2 cut(s) 162, 483
BssMI GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
BssNI GRCGYC 1 cut(s) 903
BssT1I CCWWGG 1 cut(s) 162
Bst4CI ACNGT 5 cut(s) 82, 443, 566, 795, 805
Bst6I CTCTTC 2 cut(s) 62, 266
BstACI GRCGYC 1 cut(s) 903
BstC8I GCNNGC 3 cut(s) 231, 284, 541
BstDEI CTNAG 1 cut(s) 621
BstF5I GGATG 3 cut(s) 158, 193, 750
BstFNI CGCG 1 cut(s) 834
BstKTI GATC 8 cut(s) 249, 310, 351, 490, 550, 688, 743, 783
BstMBI GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
BstSCI CCNGG 1 cut(s) 543
BstSFI CTRYAG 1 cut(s) 727
BstUI CGCG 1 cut(s) 834
BstV1I GCAGC 1 cut(s) 121
BstX2I RGATCY 2 cut(s) 547, 740
BstXI CCANNNNNNTGG 1 cut(s) 237
BstYI RGATCY 2 cut(s) 547, 740
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 2 cut(s) 229, 263
BtgZI GCGATG 1 cut(s) 872
BtsCI GGATG 3 cut(s) 158, 193, 750
BtsIMutI CAGTG 1 cut(s) 469
Cac8I GCNNGC 3 cut(s) 231, 284, 541
CciI TCATGA 1 cut(s) 391
Cfr13I GGNCC 4 cut(s) 131, 227, 261, 936
CseI GACGC 1 cut(s) 428
Csp6I GTAC 4 cut(s) 50, 511, 762, 815
CviAII CATG 7 cut(s) 267, 392, 529, 759, 845, 899, 933
CviQI GTAC 4 cut(s) 50, 511, 762, 815
DdeI CTNAG 1 cut(s) 621
DpnI GATC 8 cut(s) 248, 309, 350, 489, 549, 687, 742, 782
DpnII GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
Eam1104I CTCTTC 2 cut(s) 62, 266
EarI CTCTTC 2 cut(s) 62, 266
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 525
Eco32I GATATC 1 cut(s) 397
Eco47I GGWCC 2 cut(s) 131, 936
Eco53kI GAGCTC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 786
EcoICRI GAGCTC 1 cut(s) 523
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 7 cut(s) 270, 395, 532, 762, 848, 902, 936
FaqI GGGAC 1 cut(s) 311
FatI CATG 7 cut(s) 266, 391, 528, 758, 844, 898, 932
FauI CCCGC 1 cut(s) 863
Fnu4HI GCNGC 3 cut(s) 110, 264, 712
FokI GGATG 3 cut(s) 145, 200, 737
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 3 cut(s) 110, 264, 712
GluI GCNGC 3 cut(s) 110, 264, 712
HaeIII GGCC 2 cut(s) 229, 263
HapII CCGG 1 cut(s) 545
HgaI GACGC 1 cut(s) 428
Hin1I GRCGYC 1 cut(s) 903
Hin1II CATG 7 cut(s) 270, 395, 532, 762, 848, 902, 936
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 582, 608, 823, 859
HpaII CCGG 1 cut(s) 545
HphI GGTGA 1 cut(s) 739
Hpy188I TCNGA 4 cut(s) 307, 312, 487, 552
Hpy188III TCNNGA 5 cut(s) 176, 250, 379, 392, 605
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 5 cut(s) 34, 217, 372, 625, 761
HpyCH4III ACNGT 5 cut(s) 82, 443, 566, 795, 805
HpyCH4IV ACGT 3 cut(s) 124, 636, 903
HpyCH4V TGCA 5 cut(s) 286, 460, 539, 692, 844
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 3 cut(s) 124, 636, 903
Hsp92I GRCGYC 1 cut(s) 903
Hsp92II CATG 7 cut(s) 270, 395, 532, 762, 848, 902, 936
Kzo9I GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
LmnI GCTCC 2 cut(s) 520, 528
LpnPI CCDG 9 cut(s) 164, 243, 408, 457, 558, 618, 692, 702, 952
Lsp1109I GCAGC 1 cut(s) 121
LweI GCATC 2 cut(s) 16, 838
MaeII ACGT 3 cut(s) 124, 636, 903
MaeIII GTNAC 2 cut(s) 76, 904
MalI GATC 8 cut(s) 248, 309, 350, 489, 549, 687, 742, 782
MboI GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
MboII GAAGA 3 cut(s) 49, 283, 854
MflI RGATCY 2 cut(s) 547, 740
MhlI GDGCHC 1 cut(s) 525
MlyI GAGTC 2 cut(s) 591, 602
MmeI TCCRAC 2 cut(s) 559, 727
MnlI CCTC 6 cut(s) 165, 205, 493, 626, 765, 913
MslI CAYNNNNRTG 1 cut(s) 677
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 545
MvnI CGCG 1 cut(s) 834
NciI CCSGG 1 cut(s) 545
NdeII GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
NlaIII CATG 7 cut(s) 270, 395, 532, 762, 848, 902, 936
NlaIV GGNNCC 2 cut(s) 228, 549
NmuCI GTSAC 2 cut(s) 76, 904
PagI TCATGA 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PfeI GAWTC 2 cut(s) 823, 859
PkrI GCNGC 3 cut(s) 111, 265, 713
PleI GAGTC 2 cut(s) 590, 602
PpsI GAGTC 2 cut(s) 590, 602
Psp124BI GAGCTC 1 cut(s) 525
Psp1406I AACGTT 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 228, 549
PspPI GGNCC 4 cut(s) 131, 227, 261, 936
PsuI RGATCY 2 cut(s) 547, 740
RsaI GTAC 4 cut(s) 51, 512, 763, 816
RsaNI GTAC 4 cut(s) 50, 511, 762, 815
RseI CAYNNNNRTG 1 cut(s) 677
SacI GAGCTC 1 cut(s) 525
SatI GCNGC 3 cut(s) 110, 264, 712
Sau3AI GATC 8 cut(s) 246, 307, 348, 487, 547, 685, 740, 780
Sau96I GGNCC 4 cut(s) 131, 227, 261, 936
SchI GAGTC 2 cut(s) 591, 602
ScrFI CCNGG 1 cut(s) 545
SduI GDGCHC 1 cut(s) 525
SfaNI GCATC 2 cut(s) 16, 838
SfcI CTRYAG 1 cut(s) 727
SinI GGWCC 2 cut(s) 131, 936
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 2 cut(s) 174, 377
SmoI CTYRAG 2 cut(s) 174, 377
SsiI CCGC 5 cut(s) 107, 264, 711, 851, 870
SspI AATATT 1 cut(s) 959
SstI GAGCTC 1 cut(s) 525
StyD4I CCNGG 1 cut(s) 543
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 5 cut(s) 82, 443, 566, 795, 805
TaiI ACGT 3 cut(s) 127, 639, 906
TaqI TCGA 3 cut(s) 406, 438, 857
TaqII GACCGA 1 cut(s) 865
TatI WGTACW 2 cut(s) 49, 761
TauI GCSGC 2 cut(s) 266, 714
TfiI GAWTC 2 cut(s) 823, 859
TscAI CASTG 1 cut(s) 476
TseFI GTSAC 2 cut(s) 76, 904
TseI GCWGC 1 cut(s) 109
Tsp45I GTSAC 2 cut(s) 76, 904
TspDTI ATGAA 5 cut(s) 16, 17, 21, 644, 836
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 131, 936
ZraI GACGTC 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.