Rh5CG369800

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003273
Physical Location & Seq
Forward (+)
47192283 .. 47195613
3331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG369800.1

Sequence Viewer

Length: 699 bp
ATGAAGCATCGTGTCTGCAATGTTACTCGTTGGTTTCTTTTCCTTCTAGTTCTATCAGTCGTCACAGCTCAAGAAGTTGAGGATGAGAGAGAGTTCGATTACGTTGAGGGAAGTGAAAAGGGGCCAAATCATTGGGGACAAATGAAGAAAGAATGGGCAGCCTGCAACAATGGAGGCATGCAATCTCCCATCGATCTTTCAAACAAGAGAGTGAAGATAATACCCAAGTTGGGGAAGCTAAAAAAGAGTTACAGACCTAGTAATGCCACCGTCAAGAATAGAGGGCACGACATTTCGCTTCAATGGGAGGGCGACGCAGGATCAGTTCGGATCAACGGAACTGACTACTTGCTTAAACAATGTCACTGGCATTCGCCGTCCGAGCACTCCATCAACGGCAGGAGGTATGACATGGAGCTCCATATGGTCCATCTCAGCCCCGATCCCAATGTGACAAACAAGATTGCTGTTGTCGGAGTCCTTTACAAGTTTGGTCGCCACCCTGATTCTTTCCTCTCTAAGTTGAGGAGGCATGTAAGATCCATGACTGATCGAAAGGAACAGAGAAACATAGGGAAGATTGATCCAAGAGAAATCAAAATGGGTGGCAACAACTACTATAGATATATGGGCTCACTCACTGTTCCTCCATGTACTGAAGGTGTCATTTGGACAATCCATAAGCAGGTACATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

232

Amino Acids

26.76

Weight (kDa)

9.6

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 40 - 229 3.5e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AclWI GGATC 5 cut(s) 328, 338, 437, 534, 578
AcuI CTGAAG 1 cut(s) 678
AfaI GTAC 2 cut(s) 655, 690
AfiI CCNNNNNNNGG 3 cut(s) 230, 231, 685
AgsI TTSAA 2 cut(s) 201, 302
AluBI AGCT 3 cut(s) 68, 238, 418
AluI AGCT 3 cut(s) 68, 238, 418
Alw21I GWGCWC 2 cut(s) 387, 420
AlwI GGATC 5 cut(s) 328, 338, 437, 534, 578
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 122, 427
AvaII GGWCC 1 cut(s) 427
BaeGI GKGCMC 1 cut(s) 288
BanII GRGCYC 2 cut(s) 420, 635
Bbv12I GWGCWC 2 cut(s) 387, 420
BbvI GCAGC 1 cut(s) 170
BccI CCATC 3 cut(s) 197, 398, 438
BceAI ACGGC 2 cut(s) 361, 412
BfaI CTAG 2 cut(s) 47, 258
BfmI CTRYAG 1 cut(s) 619
BfuAI ACCTGC 1 cut(s) 676
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 122, 427
BmiI GGNNCC 1 cut(s) 123
BmsI GCATC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 54
Bsa29I ATCGAT 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 231, 685
Bse1I ACTGG 1 cut(s) 371
Bse3DI GCAATG 1 cut(s) 25
BseCI ATCGAT 1 cut(s) 192
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 3 cut(s) 230, 231, 685
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 448
BseNI ACTGG 1 cut(s) 371
BseRI GAGGAG 1 cut(s) 541
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 1 cut(s) 170
BshFI GGCC 1 cut(s) 124
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 387, 420
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 3 cut(s) 230, 231, 685
BsmFI GGGAC 1 cut(s) 150
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 124
Bsp1286I GDGCHC 4 cut(s) 288, 387, 420, 635
Bsp143I GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
BspANI GGCC 1 cut(s) 124
BspCNI CTCAG 1 cut(s) 447
BspDI ATCGAT 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 123
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 5 cut(s) 328, 338, 437, 534, 578
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 371
BssMI GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
Bst4CI ACNGT 2 cut(s) 271, 643
BstC8I GCNNGC 2 cut(s) 163, 179
BstDEI CTNAG 2 cut(s) 434, 519
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 7 cut(s) 196, 323, 333, 445, 542, 553, 586
BstMBI GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstNSI RCATGY 3 cut(s) 181, 536, 695
BstSFI CTRYAG 1 cut(s) 619
BstSLI GKGCMC 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 1 cut(s) 132
BstYI RGATCY 1 cut(s) 539
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 1 cut(s) 124
BsuTUI ATCGAT 1 cut(s) 192
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 2 cut(s) 364, 639
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 2 cut(s) 163, 179
Cfr13I GGNCC 2 cut(s) 122, 427
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 654, 689
CspCI CAANNNNNGTGG 2 cut(s) 586, 621
CviAII CATG 6 cut(s) 178, 412, 533, 544, 651, 692
CviJI RGCY 7 cut(s) 68, 124, 161, 238, 418, 438, 633
CviKI_1 RGCY 7 cut(s) 68, 124, 161, 238, 418, 438, 633
CviQI GTAC 2 cut(s) 654, 689
DdeI CTNAG 2 cut(s) 434, 519
DpnI GATC 7 cut(s) 195, 322, 332, 444, 541, 552, 585
DpnII GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
Ecl136II GAGCTC 1 cut(s) 418
Eco24I GRGCYC 2 cut(s) 420, 635
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 678
EcoICRI GAGCTC 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 697
EcoT38I GRGCYC 2 cut(s) 420, 635
FaeI CATG 6 cut(s) 181, 415, 536, 547, 654, 695
FaqI GGGAC 1 cut(s) 150
FatI CATG 6 cut(s) 177, 411, 532, 543, 650, 691
FauNDI CATATG 1 cut(s) 423
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 2 cut(s) 420, 635
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 2 cut(s) 47, 258
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 1 cut(s) 124
HgaI GACGC 1 cut(s) 323
Hin1II CATG 6 cut(s) 181, 415, 536, 547, 654, 695
HinfI GANTC 2 cut(s) 477, 506
Hpy188I TCNGA 3 cut(s) 330, 382, 476
Hpy188III TCNNGA 2 cut(s) 71, 274
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 2 cut(s) 53, 653
HpyCH4III ACNGT 2 cut(s) 271, 643
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 4 cut(s) 18, 165, 181, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 2 cut(s) 434, 519
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 6 cut(s) 181, 415, 536, 547, 654, 695
Kzo9I GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
LmnI GCTCC 2 cut(s) 415, 423
LpnPI CCDG 6 cut(s) 175, 303, 352, 385, 516, 671
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 1 cut(s) 16
MaeI CTAG 2 cut(s) 47, 258
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 5 cut(s) 22, 61, 248, 362, 451
MalI GATC 7 cut(s) 195, 322, 332, 444, 541, 552, 585
MboI GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
MboII GAAGA 3 cut(s) 157, 226, 589
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 4 cut(s) 288, 387, 420, 635
MlyI GAGTC 1 cut(s) 486
MmeI TCCRAC 1 cut(s) 454
Mph1103I ATGCAT 1 cut(s) 697
MseI TTAA 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 370
MwoI GCNNNNNNNGC 1 cut(s) 382
NdeI CATATG 1 cut(s) 423
NdeII GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
NlaIII CATG 6 cut(s) 181, 415, 536, 547, 654, 695
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 3 cut(s) 61, 362, 451
NsiI ATGCAT 1 cut(s) 697
NspI RCATGY 3 cut(s) 181, 536, 695
PaeI GCATGC 1 cut(s) 181
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 1 cut(s) 506
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 420
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 2 cut(s) 122, 427
PsuI RGATCY 1 cut(s) 539
RsaI GTAC 2 cut(s) 655, 690
RsaNI GTAC 2 cut(s) 654, 689
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 7 cut(s) 193, 320, 330, 442, 539, 550, 583
Sau96I GGNCC 2 cut(s) 122, 427
SchI GAGTC 1 cut(s) 486
SduI GDGCHC 4 cut(s) 288, 387, 420, 635
SetI ASST 8 cut(s) 70, 105, 240, 259, 407, 420, 664, 690
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 619
SinI GGWCC 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
SphI GCATGC 1 cut(s) 181
SspMI CTAG 2 cut(s) 47, 258
SstI GAGCTC 1 cut(s) 420
TaaI ACNGT 2 cut(s) 271, 643
TaiI ACGT 1 cut(s) 105
TaqI TCGA 3 cut(s) 96, 192, 553
TatI WGTACW 1 cut(s) 653
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 2 cut(s) 371, 646
TseFI GTSAC 3 cut(s) 61, 362, 451
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 3 cut(s) 61, 362, 451
TspDTI ATGAA 2 cut(s) 17, 158
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 2 cut(s) 371, 646
VpaK11BI GGWCC 1 cut(s) 427
XceI RCATGY 3 cut(s) 181, 536, 695
XspI CTAG 2 cut(s) 47, 258
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.