Rw3G015220

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003273
Physical Location & Seq
Forward (+)
15644485 .. 15646140
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015220.1

Sequence Viewer

Length: 852 bp
ATGATGATGGATCAGAGCAAGTCCATCTCCACTTCTTTATTTATGCTTCTTCTTCTTCTCCTTCAAATTTTTCACTCAACACGCATTACAGCTCAAGAAGTTGAAGAAGAAAGAGAGTTTGATTATCTGAAAGACAGTGAGAAGGGACCAAAGTATTGGGGAGAGCTTAGGGAAGAATGGGCAGCATGTAAACATGGGACACTGCAATCTCCGGTAGATTTGCTGAGTGAGAATGTGCAAGTACGCCTAAACAAAGGGGAGGTGAGACTCAAGTACAAGCCTGCAAATGCAACAATCAGAAATAGAGGCCATGACATTTCGGTCCAGTGGAATCTAGGTGATGCTGGATCCGTAGAGATTAATGGCACAGATTACTTGCTACAACAATGCCACTGGCACTCACCATCTGAGCATACAATTGATGGTATAAGATTTGACATGGAGCTTCATATGGTACACCTAATCCCTGATGCAAATGTCGAAAACAACATAGTTGTGGTTACATACCTCTACAAACTTGGCAAGCCTGATAAGTTTCTGTCGGAGGTAAGCAAGGATATCCATGCCATGATTGATGAAGAGGAAGAGAGACACAGAGGGGTGGTTGATCCAAGAGATGTGAAGAAGGGTGGCAAAATTTTTTACAAGTACATAGGCTCACTCACTGTACCTCCTTGCACTGAAGGAGTAACTTGGATCATCCACAAAAAGTTAACTACTGTTTCAAGAGAGCAAATGGAGTTGCTTCGAGAAGCCGTTCATGACTTCGCAGAACAGAATGCAAGACCATTGCAAGCTCTCAATCGCCGAGATGTGCACTTCCATGGCCCTGAGTCTCACATTCGGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

283

Amino Acids

32.71

Weight (kDa)

5.93

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 49 - 273 1.1e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AclWI GGATC 5 cut(s) 18, 342, 355, 602, 704
AcsI RAATTY 2 cut(s) 66, 636
AcuI CTGAAG 1 cut(s) 702
AfaI GTAC 5 cut(s) 243, 275, 456, 650, 669
AgsI TTSAA 3 cut(s) 65, 104, 726
AluBI AGCT 4 cut(s) 92, 166, 445, 797
AluI AGCT 4 cut(s) 92, 166, 445, 797
Alw21I GWGCWC 1 cut(s) 819
Alw26I GTCTC 3 cut(s) 259, 583, 840
Alw44I GTGCAC 1 cut(s) 815
AlwI GGATC 5 cut(s) 18, 342, 355, 602, 704
AoxI GGCC 2 cut(s) 307, 826
ApaLI GTGCAC 1 cut(s) 815
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 2 cut(s) 66, 636
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 756
AspS9I GGNCC 3 cut(s) 146, 322, 827
AsuHPI GGTGA 3 cut(s) 274, 350, 393
AvaII GGWCC 2 cut(s) 146, 322
BaeGI GKGCMC 1 cut(s) 819
BamHI GGATCC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 819
BbvI GCAGC 1 cut(s) 194
BccI CCATC 3 cut(s) 32, 412, 416
BceAI ACGGC 1 cut(s) 740
BcoDI GTCTC 3 cut(s) 259, 583, 840
BfaI CTAG 1 cut(s) 335
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme18I GGWCC 2 cut(s) 146, 322
BmgT120I GGNCC 3 cut(s) 146, 322, 827
BmiI GGNNCC 2 cut(s) 147, 349
BmsI GCATC 2 cut(s) 331, 460
Bpu10I CCTNAGC 1 cut(s) 167
BpuEI CTTGAG 2 cut(s) 78, 254
BsaJI CCNNGG 1 cut(s) 823
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bse1I ACTGG 2 cut(s) 325, 398
Bse3DI GCAATG 1 cut(s) 788
BseDI CCNNGG 1 cut(s) 823
BseGI GGATG 1 cut(s) 699
BseMI GCAATG 1 cut(s) 788
BseMII CTCAG 3 cut(s) 215, 399, 822
BseNI ACTGG 2 cut(s) 325, 398
BseSI GKGCMC 1 cut(s) 819
BseXI GCAGC 1 cut(s) 194
BshFI GGCC 2 cut(s) 309, 828
BsiHKAI GWGCWC 1 cut(s) 819
BsiSI CCGG 1 cut(s) 212
BslFI GGGAC 2 cut(s) 159, 211
BsmAI GTCTC 3 cut(s) 259, 583, 840
BsmFI GGGAC 2 cut(s) 159, 211
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 2 cut(s) 309, 828
Bsp1286I GDGCHC 1 cut(s) 819
Bsp143I GATC 4 cut(s) 10, 347, 607, 696
Bsp19I CCATGG 1 cut(s) 823
BspANI GGCC 2 cut(s) 309, 828
BspCNI CTCAG 3 cut(s) 216, 400, 823
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 2 cut(s) 147, 349
BspPI GGATC 5 cut(s) 18, 342, 355, 602, 704
BsrDI GCAATG 1 cut(s) 788
BsrI ACTGG 2 cut(s) 325, 398
BssECI CCNNGG 1 cut(s) 823
BssMI GATC 4 cut(s) 10, 347, 607, 696
BssT1I CCWWGG 1 cut(s) 823
Bst4CI ACNGT 3 cut(s) 137, 667, 721
Bst6I CTCTTC 2 cut(s) 573, 579
BstC8I GCNNGC 3 cut(s) 282, 524, 795
BstDEI CTNAG 4 cut(s) 167, 224, 408, 831
BstDSI CCRYGG 1 cut(s) 823
BstF5I GGATG 1 cut(s) 699
BstKTI GATC 4 cut(s) 13, 350, 610, 699
BstMAI GTCTC 3 cut(s) 259, 583, 840
BstMBI GATC 4 cut(s) 10, 347, 607, 696
BstNSI RCATGY 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 819
BstV1I GCAGC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 156
BstYI RGATCY 1 cut(s) 347
BsuRI GGCC 2 cut(s) 309, 828
BtgI CCRYGG 1 cut(s) 823
BtsCI GGATG 1 cut(s) 699
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 6 cut(s) 142, 200, 332, 391, 663, 678
Cac8I GCNNGC 3 cut(s) 282, 524, 795
CciI TCATGA 1 cut(s) 760
Cfr13I GGNCC 3 cut(s) 146, 322, 827
Csp6I GTAC 5 cut(s) 242, 274, 455, 649, 668
CviAII CATG 8 cut(s) 186, 194, 311, 439, 563, 568, 761, 824
CviQI GTAC 5 cut(s) 242, 274, 455, 649, 668
DdeI CTNAG 4 cut(s) 167, 224, 408, 831
DpnI GATC 4 cut(s) 12, 349, 609, 698
DpnII GATC 4 cut(s) 10, 347, 607, 696
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
Eam1104I CTCTTC 2 cut(s) 573, 579
EarI CTCTTC 2 cut(s) 573, 579
Eco130I CCWWGG 1 cut(s) 823
Eco32I GATATC 1 cut(s) 559
Eco47I GGWCC 2 cut(s) 146, 322
Eco57I CTGAAG 1 cut(s) 702
EcoRV GATATC 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 823
ErhI CCWWGG 1 cut(s) 823
FaeI CATG 8 cut(s) 189, 197, 314, 442, 566, 571, 764, 827
FaqI GGGAC 2 cut(s) 159, 211
FatI CATG 8 cut(s) 185, 193, 310, 438, 562, 567, 760, 823
FauNDI CATATG 1 cut(s) 450
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 1 cut(s) 686
Fsp4HI GCNGC 1 cut(s) 183
FspBI CTAG 1 cut(s) 335
GluI GCNGC 1 cut(s) 183
HaeIII GGCC 2 cut(s) 309, 828
HapII CCGG 1 cut(s) 212
Hin1II CATG 8 cut(s) 189, 197, 314, 442, 566, 571, 764, 827
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HinfI GANTC 3 cut(s) 267, 331, 833
HpaI GTTAAC 1 cut(s) 714
HpaII CCGG 1 cut(s) 212
HphI GGTGA 3 cut(s) 274, 350, 393
Hpy166II GTNNAC 4 cut(s) 191, 457, 714, 817
Hpy188I TCNGA 5 cut(s) 15, 129, 299, 409, 544
Hpy188III TCNNGA 5 cut(s) 95, 726, 749, 761, 845
Hpy8I GTNNAC 4 cut(s) 191, 457, 714, 817
HpyAV CCTTC 4 cut(s) 71, 136, 619, 677
HpyCH4III ACNGT 3 cut(s) 137, 667, 721
HpyCH4V TGCA 9 cut(s) 205, 238, 284, 290, 473, 678, 782, 793, 817
HpyF3I CTNAG 4 cut(s) 167, 224, 408, 831
Hsp92II CATG 8 cut(s) 189, 197, 314, 442, 566, 571, 764, 827
KspAI GTTAAC 1 cut(s) 714
Kzo9I GATC 4 cut(s) 10, 347, 607, 696
LmnI GCTCC 1 cut(s) 442
LpnPI CCDG 8 cut(s) 225, 294, 330, 338, 379, 480, 540, 843
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 2 cut(s) 331, 460
MaeI CTAG 1 cut(s) 335
MaeIII GTNAC 2 cut(s) 499, 688
MalI GATC 4 cut(s) 12, 349, 609, 698
MboI GATC 4 cut(s) 10, 347, 607, 696
MboII GAAGA 9 cut(s) 41, 44, 47, 116, 119, 185, 590, 596, 634
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 1 cut(s) 819
MluCI AATT 3 cut(s) 66, 417, 636
MlyI GAGTC 2 cut(s) 261, 842
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 7 cut(s) 253, 299, 518, 538, 574, 590, 681
MroXI GAANNNNTTC 1 cut(s) 756
MseI TTAA 3 cut(s) 360, 713, 850
MslI CAYNNNNRTG 2 cut(s) 494, 822
MspI CCGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 417
Mva1269I GAATGC 1 cut(s) 784
NcoI CCATGG 1 cut(s) 823
NdeI CATATG 1 cut(s) 450
NdeII GATC 4 cut(s) 10, 347, 607, 696
NlaIII CATG 8 cut(s) 189, 197, 314, 442, 566, 571, 764, 827
NlaIV GGNNCC 2 cut(s) 147, 349
NmeAIII GCCGAG 1 cut(s) 833
NspI RCATGY 1 cut(s) 189
PagI TCATGA 1 cut(s) 760
PctI GAATGC 1 cut(s) 784
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 1 cut(s) 184
PleI GAGTC 2 cut(s) 261, 841
PpsI GAGTC 2 cut(s) 261, 841
PshBI ATTAAT 1 cut(s) 360
PspN4I GGNNCC 2 cut(s) 147, 349
PspPI GGNCC 3 cut(s) 146, 322, 827
PsuI RGATCY 1 cut(s) 347
RsaI GTAC 5 cut(s) 243, 275, 456, 650, 669
RsaNI GTAC 5 cut(s) 242, 274, 455, 649, 668
RseI CAYNNNNRTG 2 cut(s) 494, 822
SaqAI TTAA 3 cut(s) 360, 713, 850
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 4 cut(s) 10, 347, 607, 696
Sau96I GGNCC 3 cut(s) 146, 322, 827
SchI GAGTC 2 cut(s) 261, 842
SduI GDGCHC 1 cut(s) 819
SfaNI GCATC 2 cut(s) 331, 460
SinI GGWCC 2 cut(s) 146, 322
SmiMI CAYNNNNRTG 2 cut(s) 494, 822
SmlI CTYRAG 2 cut(s) 93, 269
SmoI CTYRAG 2 cut(s) 93, 269
Sse9I AATT 3 cut(s) 66, 417, 636
SspMI CTAG 1 cut(s) 335
StyI CCWWGG 1 cut(s) 823
TaaI ACNGT 3 cut(s) 137, 667, 721
TaqI TCGA 2 cut(s) 480, 748
TaqII GACCGA 1 cut(s) 310
TasI AATT 3 cut(s) 66, 417, 636
TatI WGTACW 2 cut(s) 273, 648
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 3 cut(s) 360, 713, 850
Tru9I TTAA 3 cut(s) 360, 713, 850
TscAI CASTG 6 cut(s) 142, 207, 332, 398, 670, 685
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 3 cut(s) 437, 591, 749
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 6 cut(s) 142, 207, 332, 398, 670, 685
VneI GTGCAC 1 cut(s) 815
VpaK11BI GGWCC 2 cut(s) 146, 322
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 2 cut(s) 66, 636
XceI RCATGY 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.