Rh5BG342600

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003273
Physical Location & Seq
Reverse (-)
51720211 .. 51721572
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG342600.1

Sequence Viewer

Length: 834 bp
ATGAAGCATCGCGTCTGCAATGTTAGTTTCTGGTTTCTTTTCCTTGTAGTTCTATCAGTCGTCACAGCTCAAGAAGTTGAGGATGAGAGAGAGTTCGATTACGTTGAGGGAAGTGAAAAGGGGCCAAATCATTGGGGAGAAATGAAGAAGGAATGGGCAGCCTGTAACAATGGAGGCATGCAATCTCCCATCGATCTTTCAAACAAGAGAGTGAAGATAATACCCAAGTTGGGGAAGCTAAAAAAGAGTTACAGACCTAGTAATGCCACCGTCAAGAATAGAGGGCACGACATTTCGCTTCAATGGGAGGGCGACGCAGGATCAGTTCGGATCAACGGAACTGAATACTTGCTTAAACAATGTCACTGGCATTCGCCGTCTGAGCACTCCATCAACGGCAGGAGGTATGACATGGAGCTCCATATGGTCCATCTCAGCCCCGATCCCAATGTGACAAACAAGATTGCTGTCGTCGGAGTCCTTTACAAGTTTGGTCGCCACCCTGATTCTTTCCTCTCTAAGTTGAGGGGGCATGTAAGATCCATGACTGATCGAAAGGAACATAGAAACATAGGGAAGATTGATCCAAGAGAAATCAAAATGGGTGGCAACAACTACTATAGATATATGGGCTCACTCACTGTTCCTCCATGTACTGAAGGTGTCATTTGGACAATCCATAAGCAGATAAGAAGTGTCTCGAGGGAACAAGTAAAACTATTTCGTTCGGCTGTGCACGATTATGCAGAGAGGAATGCGAGGCCAGTACAACCACTCAACCTCCGAGAAATAGAGATGTATGATCAAACAGCAAATCAGGAGCAACACCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

277

Amino Acids

32.08

Weight (kDa)

9.33

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 40 - 265 5.1e-46 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 12
AclWI GGATC 5 cut(s) 328, 338, 437, 534, 578
AcuI CTGAAG 1 cut(s) 678
AfaI GTAC 2 cut(s) 655, 768
AfiI CCNNNNNNNGG 2 cut(s) 230, 231
AgsI TTSAA 2 cut(s) 201, 302
AluBI AGCT 3 cut(s) 68, 238, 418
AluI AGCT 3 cut(s) 68, 238, 418
Alw21I GWGCWC 3 cut(s) 387, 420, 738
Alw26I GTCTC 1 cut(s) 703
Alw44I GTGCAC 1 cut(s) 734
AlwI GGATC 5 cut(s) 328, 338, 437, 534, 578
Ama87I CYCGRG 1 cut(s) 700
AoxI GGCC 2 cut(s) 122, 761
ApaLI GTGCAC 1 cut(s) 734
ApeKI GCWGC 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 122, 427
AvaI CYCGRG 1 cut(s) 700
AvaII GGWCC 1 cut(s) 427
BaeGI GKGCMC 2 cut(s) 288, 738
BanII GRGCYC 2 cut(s) 420, 635
Bbv12I GWGCWC 3 cut(s) 387, 420, 738
BbvI GCAGC 1 cut(s) 170
BccI CCATC 3 cut(s) 197, 398, 438
BceAI ACGGC 2 cut(s) 361, 412
BclI TGATCA 1 cut(s) 802
BcoDI GTCTC 1 cut(s) 703
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 619
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme18I GGWCC 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 700
BmgT120I GGNCC 2 cut(s) 122, 427
BmiI GGNNCC 1 cut(s) 123
BmsI GCATC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 54
Bsa29I ATCGAT 1 cut(s) 192
BsaXI ACNNNNNCTCC 4 cut(s) 631, 661, 765, 795
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 231
Bse1I ACTGG 2 cut(s) 371, 764
Bse3DI GCAATG 1 cut(s) 25
BseCI ATCGAT 1 cut(s) 192
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 2 cut(s) 230, 231
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 372, 448
BseNI ACTGG 2 cut(s) 371, 764
BseSI GKGCMC 2 cut(s) 288, 738
BseXI GCAGC 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 124, 763
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 3 cut(s) 387, 420, 738
BsiHKCI CYCGRG 1 cut(s) 700
BslI CCNNNNNNNGG 2 cut(s) 230, 231
BsmAI GTCTC 1 cut(s) 703
BsmI GAATGC 2 cut(s) 370, 760
BsnI GGCC 2 cut(s) 124, 763
BsoBI CYCGRG 1 cut(s) 700
Bsp1286I GDGCHC 5 cut(s) 288, 387, 420, 635, 738
Bsp143I GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
BspANI GGCC 2 cut(s) 124, 763
BspCNI CTCAG 2 cut(s) 373, 447
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 5 cut(s) 328, 338, 437, 534, 578
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 2 cut(s) 371, 764
BssMI GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
Bst4CI ACNGT 2 cut(s) 271, 643
BstC8I GCNNGC 1 cut(s) 179
BstDEI CTNAG 3 cut(s) 381, 434, 519
BstF5I GGATG 1 cut(s) 88
BstFNI CGCG 1 cut(s) 12
BstKTI GATC 8 cut(s) 196, 323, 333, 445, 542, 553, 586, 805
BstMAI GTCTC 1 cut(s) 703
BstMBI GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstNSI RCATGY 2 cut(s) 181, 536
BstSFI CTRYAG 1 cut(s) 619
BstSLI GKGCMC 2 cut(s) 288, 738
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 1 cut(s) 132
BstYI RGATCY 1 cut(s) 539
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 2 cut(s) 124, 763
BsuTUI ATCGAT 1 cut(s) 192
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 2 cut(s) 364, 639
Cac8I GCNNGC 1 cut(s) 179
Cfr13I GGNCC 2 cut(s) 122, 427
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 654, 767
CspCI CAANNNNNGTGG 2 cut(s) 586, 621
CviAII CATG 5 cut(s) 178, 412, 533, 544, 651
CviJI RGCY 9 cut(s) 68, 124, 161, 238, 418, 438, 633, 731, 763
CviKI_1 RGCY 9 cut(s) 68, 124, 161, 238, 418, 438, 633, 731, 763
CviQI GTAC 2 cut(s) 654, 767
DdeI CTNAG 3 cut(s) 381, 434, 519
DpnI GATC 8 cut(s) 195, 322, 332, 444, 541, 552, 585, 804
DpnII GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
Ecl136II GAGCTC 1 cut(s) 418
Eco24I GRGCYC 2 cut(s) 420, 635
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 678
Eco88I CYCGRG 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 418
EcoT38I GRGCYC 2 cut(s) 420, 635
FaeI CATG 5 cut(s) 181, 415, 536, 547, 654
FatI CATG 5 cut(s) 177, 411, 532, 543, 650
FauNDI CATATG 1 cut(s) 423
FbaI TGATCA 1 cut(s) 802
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 2 cut(s) 420, 635
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 258
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 2 cut(s) 124, 763
HgaI GACGC 1 cut(s) 323
Hin1II CATG 5 cut(s) 181, 415, 536, 547, 654
HinfI GANTC 2 cut(s) 477, 506
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 4 cut(s) 330, 382, 476, 785
Hpy188III TCNNGA 4 cut(s) 71, 274, 700, 818
Hpy8I GTNNAC 1 cut(s) 736
Hpy99I CGWCG 2 cut(s) 317, 476
HpyAV CCTTC 2 cut(s) 142, 653
HpyCH4III ACNGT 2 cut(s) 271, 643
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 4 cut(s) 18, 181, 736, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 3 cut(s) 381, 434, 519
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 5 cut(s) 181, 415, 536, 547, 654
Ksp22I TGATCA 1 cut(s) 802
Kzo9I GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
LmnI GCTCC 3 cut(s) 415, 423, 820
LpnPI CCDG 8 cut(s) 16, 175, 303, 352, 385, 516, 777, 803
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 1 cut(s) 16
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 5 cut(s) 61, 164, 248, 362, 451
MalI GATC 8 cut(s) 195, 322, 332, 444, 541, 552, 585, 804
MboI GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
MboII GAAGA 3 cut(s) 157, 226, 589
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 5 cut(s) 288, 387, 420, 635, 738
MlyI GAGTC 1 cut(s) 486
MmeI TCCRAC 1 cut(s) 454
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 741
Mva1269I GAATGC 2 cut(s) 370, 760
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 382
NdeI CATATG 1 cut(s) 423
NdeII GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
NlaIII CATG 5 cut(s) 181, 415, 536, 547, 654
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 3 cut(s) 61, 362, 451
NspI RCATGY 2 cut(s) 181, 536
PaeI GCATGC 1 cut(s) 181
PaeR7I CTCGAG 1 cut(s) 700
PctI GAATGC 2 cut(s) 370, 760
PfeI GAWTC 1 cut(s) 506
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 420
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 2 cut(s) 122, 427
PsuI RGATCY 1 cut(s) 539
RsaI GTAC 2 cut(s) 655, 768
RsaNI GTAC 2 cut(s) 654, 767
RseI CAYNNNNRTG 1 cut(s) 741
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 8 cut(s) 193, 320, 330, 442, 539, 550, 583, 802
Sau96I GGNCC 2 cut(s) 122, 427
SchI GAGTC 1 cut(s) 486
SduI GDGCHC 5 cut(s) 288, 387, 420, 635, 738
SetI ASST 8 cut(s) 70, 105, 240, 259, 407, 420, 664, 783
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 619
Sfr274I CTCGAG 1 cut(s) 700
SinI GGWCC 1 cut(s) 427
SlaI CTCGAG 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 2 cut(s) 69, 700
SmoI CTYRAG 2 cut(s) 69, 700
SphI GCATGC 1 cut(s) 181
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 420
TaaI ACNGT 2 cut(s) 271, 643
TaiI ACGT 1 cut(s) 105
TaqI TCGA 4 cut(s) 96, 192, 553, 701
TatI WGTACW 2 cut(s) 653, 766
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 2 cut(s) 371, 646
TseFI GTSAC 3 cut(s) 61, 362, 451
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 3 cut(s) 61, 362, 451
TspDTI ATGAA 2 cut(s) 17, 158
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 2 cut(s) 371, 646
VneI GTGCAC 1 cut(s) 734
VpaK11BI GGWCC 1 cut(s) 427
XceI RCATGY 2 cut(s) 181, 536
XhoI CTCGAG 1 cut(s) 700
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.