MD03G1165000.v1.1

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003273
Physical Location & Seq
Reverse (-)
21774747 .. 21779147
4401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1165000.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGAGCCCCATCGAAGCAAACCCATCTCAAATTCTTGTTTTCTTTTCTTTTTACTTCTATTCGCGTATTCCAGATCACTCACAGCTCAAGAAGTCGAGGATGAAAGAGAGTTTGATTATCTTCGGGAGGGTGGAAAGGGGCCAAAGCATTGGGGAGAGATTAAGGAAGAATGGGCGGCGTGTAACAACGGGAGCATGCAATCTCCAATAGATTTATCAAATCACAGGGTTACATTATTCCCAAACTTAGGGAAGCTCAAGACCAACTACAGACCTCGTAATGCAACTATCAAGAACAGAGGCCATGATATTTCGGTTGAATGGGTGGGCGACGCTGGAACAATTAAGATAAATGGTACTGAGTATCCGCTAAAACAATGTCACTGGCATGCCCCAGCGGAGCATTCCATCAACGGCAGAATATATGACATGGAGCTCCACTTGGTTCATCTAAGCCCCGATCCCAACGTAGCAAACAAGATCGCTGTCACTGCAGTTCTCTACAAGTTTGGTCGTGCAGATTCTTTCCTTTCTACGTTGATGAAGGACGTAGAATCGATGATTGATCAAAAGGAAGAGAGAAGCATGGGCATTATTGATCCTAGAGAAATCAAAATTCGTGGGGAATCCTATTACAGATACATGGGTTCACTCACTGTTCCTCCTTGCACTGAAGGCGTTATTTGGACCATCAATAAAAAGTATGCGGAGGCGAATGCAAGGCCAGTGCAACCACACAACCTTCGAGATATCAAGGTCTATCACAAAAGCGCAAGGAGCACAAATAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

265

Amino Acids

30.29

Weight (kDa)

8.74

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 46 - 241 1.5e-35 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 65
AciI CCGC 4 cut(s) 176, 368, 398, 707
AclWI GGATC 2 cut(s) 455, 593
AcsI RAATTY 2 cut(s) 31, 615
AcuI CTGAAG 1 cut(s) 693
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 1 cut(s) 248
AgsI TTSAA 1 cut(s) 320
AluBI AGCT 3 cut(s) 86, 256, 436
AluI AGCT 3 cut(s) 86, 256, 436
Alw21I GWGCWC 2 cut(s) 438, 782
AlwI GGATC 2 cut(s) 455, 593
AoxI GGCC 3 cut(s) 140, 301, 722
ApoI RAATTY 2 cut(s) 31, 615
AspLEI GCGC 1 cut(s) 773
AspS9I GGNCC 2 cut(s) 140, 687
AvaII GGWCC 1 cut(s) 687
BaeI ACNNNNGTAYC 2 cut(s) 631, 664
BanII GRGCYC 2 cut(s) 9, 438
Bbv12I GWGCWC 2 cut(s) 438, 782
BccI CCATC 4 cut(s) 18, 32, 416, 698
BceAI ACGGC 1 cut(s) 430
BciVI GTATCC 1 cut(s) 375
BclI TGATCA 1 cut(s) 565
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 2 cut(s) 268, 492
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme18I GGWCC 1 cut(s) 687
BmgT120I GGNCC 2 cut(s) 140, 687
BmiI GGNNCC 2 cut(s) 6, 141
BpuEI CTTGAG 2 cut(s) 72, 242
Bsa29I ATCGAT 1 cut(s) 557
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse1I ACTGG 2 cut(s) 389, 725
BseCI ATCGAT 1 cut(s) 557
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 2 cut(s) 389, 725
BseYI CCCAGC 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 142, 303, 724
BshVI ATCGAT 1 cut(s) 557
BsiHKAI GWGCWC 2 cut(s) 438, 782
BslI CCNNNNNNNGG 1 cut(s) 248
BsmI GAATGC 2 cut(s) 403, 721
BsnI GGCC 3 cut(s) 142, 303, 724
Bsp1286I GDGCHC 3 cut(s) 9, 438, 782
Bsp143I GATC 5 cut(s) 74, 460, 480, 565, 598
BspACI CCGC 4 cut(s) 176, 368, 398, 707
BspANI GGCC 3 cut(s) 142, 303, 724
BspCNI CTCAG 1 cut(s) 352
BspDI ATCGAT 1 cut(s) 557
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 2 cut(s) 6, 141
BspMAI CTGCAG 1 cut(s) 496
BspPI GGATC 2 cut(s) 455, 593
BsrI ACTGG 2 cut(s) 389, 725
BssMI GATC 5 cut(s) 74, 460, 480, 565, 598
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 1 cut(s) 570
BstC8I GCNNGC 2 cut(s) 197, 390
BstDEI CTNAG 3 cut(s) 247, 360, 452
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 773
BstKTI GATC 5 cut(s) 77, 463, 483, 568, 601
BstMBI GATC 5 cut(s) 74, 460, 480, 565, 598
BstMWI GCNNNNNNNGC 3 cut(s) 491, 675, 777
BstNSI RCATGY 2 cut(s) 199, 392
BstSFI CTRYAG 2 cut(s) 268, 492
BstUI CGCG 1 cut(s) 65
BstXI CCANNNNNNTGG 1 cut(s) 150
Bsu15I ATCGAT 1 cut(s) 557
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 3 cut(s) 142, 303, 724
BsuTUI ATCGAT 1 cut(s) 557
BtsCI GGATG 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 5 cut(s) 382, 489, 654, 669, 732
Cac8I GCNNGC 2 cut(s) 197, 390
CfoI GCGC 1 cut(s) 773
Cfr13I GGNCC 2 cut(s) 140, 687
ClaI ATCGAT 1 cut(s) 557
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 357
CspCI CAANNNNNGTGG 2 cut(s) 601, 636
CviAII CATG 6 cut(s) 196, 305, 389, 430, 586, 643
CviJI RGCY 8 cut(s) 7, 86, 142, 256, 303, 436, 456, 724
CviKI_1 RGCY 8 cut(s) 7, 86, 142, 256, 303, 436, 456, 724
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 3 cut(s) 247, 360, 452
DpnI GATC 5 cut(s) 76, 462, 482, 567, 600
DpnII GATC 5 cut(s) 74, 460, 480, 565, 598
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Ecl136II GAGCTC 1 cut(s) 436
Eco24I GRGCYC 2 cut(s) 9, 438
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 687
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 693
EcoICRI GAGCTC 1 cut(s) 436
EcoRV GATATC 1 cut(s) 751
EcoT38I GRGCYC 2 cut(s) 9, 438
FaeI CATG 6 cut(s) 199, 308, 392, 433, 589, 646
FaiI YATR 9 cut(s) 197, 306, 390, 424, 426, 431, 587, 644, 705
FatI CATG 6 cut(s) 195, 304, 388, 429, 585, 642
FbaI TGATCA 1 cut(s) 565
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 2 cut(s) 9, 438
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 603
GlaI GCGC 1 cut(s) 772
GluI GCNGC 1 cut(s) 177
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 3 cut(s) 142, 303, 724
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 773
Hin1II CATG 6 cut(s) 199, 308, 392, 433, 589, 646
Hin6I GCGC 1 cut(s) 771
HinP1I GCGC 1 cut(s) 771
HinfI GANTC 3 cut(s) 521, 554, 626
Hpy166II GTNNAC 1 cut(s) 650
Hpy188III TCNNGA 6 cut(s) 72, 89, 125, 259, 292, 746
Hpy8I GTNNAC 1 cut(s) 650
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 3 cut(s) 538, 668, 752
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4IV ACGT 3 cut(s) 468, 536, 549
HpyCH4V TGCA 7 cut(s) 199, 284, 494, 518, 669, 719, 730
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 675, 777
HpyF3I CTNAG 3 cut(s) 247, 360, 452
HpySE526I ACGT 3 cut(s) 468, 536, 549
Hsp92II CATG 6 cut(s) 199, 308, 392, 433, 589, 646
HspAI GCGC 1 cut(s) 771
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 5 cut(s) 74, 460, 480, 565, 598
LmnI GCTCC 6 cut(s) 4, 192, 400, 433, 441, 777
LpnPI CCDG 6 cut(s) 85, 211, 321, 370, 408, 738
MaeI CTAG 1 cut(s) 603
MaeII ACGT 3 cut(s) 468, 536, 549
MaeIII GTNAC 4 cut(s) 182, 229, 380, 487
MalI GATC 5 cut(s) 76, 462, 482, 567, 600
MboI GATC 5 cut(s) 74, 460, 480, 565, 598
MboII GAAGA 3 cut(s) 113, 179, 587
MhlI GDGCHC 3 cut(s) 9, 438, 782
MluCI AATT 3 cut(s) 31, 342, 615
MnlI CCTC 6 cut(s) 91, 121, 285, 293, 672, 703
MseI TTAA 2 cut(s) 162, 345
MslI CAYNNNNRTG 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 398
Mva1269I GAATGC 2 cut(s) 403, 721
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 3 cut(s) 491, 675, 777
NdeII GATC 5 cut(s) 74, 460, 480, 565, 598
NlaIII CATG 6 cut(s) 199, 308, 392, 433, 589, 646
NlaIV GGNNCC 2 cut(s) 6, 141
NmuCI GTSAC 2 cut(s) 380, 487
NspI RCATGY 2 cut(s) 199, 392
PaeI GCATGC 2 cut(s) 199, 392
PctI GAATGC 2 cut(s) 403, 721
PfeI GAWTC 3 cut(s) 521, 554, 626
PkrI GCNGC 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 438
PspFI CCCAGC 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 6, 141
PspPI GGNCC 2 cut(s) 140, 687
PstI CTGCAG 1 cut(s) 496
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 2 cut(s) 162, 345
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 5 cut(s) 74, 460, 480, 565, 598
Sau96I GGNCC 2 cut(s) 140, 687
SduI GDGCHC 3 cut(s) 9, 438, 782
SetI ASST 9 cut(s) 88, 258, 277, 438, 471, 539, 552, 744, 759
SfcI CTRYAG 2 cut(s) 268, 492
SinI GGWCC 1 cut(s) 687
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 87, 257
SmoI CTYRAG 2 cut(s) 87, 257
SphI GCATGC 2 cut(s) 199, 392
Sse9I AATT 3 cut(s) 31, 342, 615
SsiI CCGC 4 cut(s) 176, 368, 398, 707
SspMI CTAG 1 cut(s) 603
SstI GAGCTC 1 cut(s) 438
TaaI ACNGT 1 cut(s) 658
TaiI ACGT 3 cut(s) 471, 539, 552
TaqI TCGA 4 cut(s) 13, 96, 557, 745
TasI AATT 3 cut(s) 31, 342, 615
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 3 cut(s) 521, 554, 626
Tru1I TTAA 2 cut(s) 162, 345
Tru9I TTAA 2 cut(s) 162, 345
TscAI CASTG 5 cut(s) 389, 496, 661, 676, 732
TseFI GTSAC 2 cut(s) 380, 487
Tsp45I GTSAC 2 cut(s) 380, 487
TspDTI ATGAA 3 cut(s) 117, 437, 557
TspRI CASTG 5 cut(s) 389, 496, 661, 676, 732
VpaK11BI GGWCC 1 cut(s) 687
XapI RAATTY 2 cut(s) 31, 615
XceI RCATGY 2 cut(s) 199, 392
XspI CTAG 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.