Rh5DG355400

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003273
Physical Location & Seq
Reverse (-)
52474983 .. 52477008
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG355400.1

Sequence Viewer

Length: 630 bp
ATGGATGCAGAGGATGAGAGAGAGTTCGATTACGTTGAGGGAAGTGAAAAGGGGCCAAATCATTGGGGAGAAATGAAGAAGGAATGGGCAGCCTGTAACAATGGAGGCATGCAATCTCCCATCGATCTTTCAAACAAGAGAGTGAAGATAATACCCAAGTTGGGGAAGCTAAAAAAGAGTTACAGACCTAGTAATGCCACCGTCAAGAATAGAGGGCACGACATTTCGCTTCAATGGGAGGGCGACGCAGGATCAGTTCGGATCAACGGAACTGAATACTTGCTTAAACAATGTCACTGGCATTCGCCGTCTGAGCACTCCATCAACGGCAGGAGGTATGACATGGAGCTCCATATGGTCCATCTCAGCCCCGATCCCAATGTGACAAACAAGATTGCTGTCGTCGGAGTCCTTTACAAGTTTGGTCGCCACCCTGATTCTTTCCTCTCTAAGTTGAGGGGGCATGTAAGATCCATGACTGATCGAAAGGAACATAGAAACATAGGGAAGATTGATCCAAGAGAAATCAAAATGGGTGGCAACAACTACTATAGATATATGGGCTCACTCACTGTTCCTCCATGTACTGAAGGTGTCATTTGGACAATCCATAAGCAGGTACATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

209

Amino Acids

23.93

Weight (kDa)

9.27

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 17 - 206 5.8e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 607
AclWI GGATC 5 cut(s) 259, 269, 368, 465, 509
AcuI CTGAAG 1 cut(s) 609
AfaI GTAC 2 cut(s) 586, 621
AfiI CCNNNNNNNGG 3 cut(s) 161, 162, 616
AgsI TTSAA 2 cut(s) 132, 233
AluBI AGCT 2 cut(s) 169, 349
AluI AGCT 2 cut(s) 169, 349
Alw21I GWGCWC 2 cut(s) 318, 351
AlwI GGATC 5 cut(s) 259, 269, 368, 465, 509
AoxI GGCC 1 cut(s) 53
ApeKI GCWGC 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 53, 358
AvaII GGWCC 1 cut(s) 358
BaeGI GKGCMC 1 cut(s) 219
BanII GRGCYC 2 cut(s) 351, 566
Bbv12I GWGCWC 2 cut(s) 318, 351
BbvI GCAGC 1 cut(s) 101
BccI CCATC 3 cut(s) 128, 329, 369
BceAI ACGGC 2 cut(s) 292, 343
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 607
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 53, 358
BmiI GGNNCC 1 cut(s) 54
Bsa29I ATCGAT 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 562, 592
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 162, 616
Bse1I ACTGG 1 cut(s) 302
BseCI ATCGAT 1 cut(s) 123
BseGI GGATG 2 cut(s) 10, 19
BseLI CCNNNNNNNGG 3 cut(s) 161, 162, 616
BseMII CTCAG 2 cut(s) 303, 379
BseNI ACTGG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 1 cut(s) 101
BshFI GGCC 1 cut(s) 55
BshVI ATCGAT 1 cut(s) 123
BsiHKAI GWGCWC 2 cut(s) 318, 351
BslI CCNNNNNNNGG 3 cut(s) 161, 162, 616
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 1 cut(s) 55
Bsp1286I GDGCHC 4 cut(s) 219, 318, 351, 566
Bsp143I GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 2 cut(s) 304, 378
BspDI ATCGAT 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 54
BspMI ACCTGC 1 cut(s) 607
BspPI GGATC 5 cut(s) 259, 269, 368, 465, 509
BsrI ACTGG 1 cut(s) 302
BssMI GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
Bst4CI ACNGT 2 cut(s) 202, 574
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 3 cut(s) 312, 365, 450
BstF5I GGATG 2 cut(s) 10, 19
BstKTI GATC 7 cut(s) 127, 254, 264, 376, 473, 484, 517
BstMBI GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstNSI RCATGY 3 cut(s) 112, 467, 626
BstSFI CTRYAG 1 cut(s) 550
BstSLI GKGCMC 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 63
BstYI RGATCY 1 cut(s) 470
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 1 cut(s) 55
BsuTUI ATCGAT 1 cut(s) 123
BtsCI GGATG 2 cut(s) 10, 19
BtsIMutI CAGTG 2 cut(s) 295, 570
BveI ACCTGC 1 cut(s) 607
Cac8I GCNNGC 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 53, 358
ClaI ATCGAT 1 cut(s) 123
CseI GACGC 1 cut(s) 254
Csp6I GTAC 2 cut(s) 585, 620
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 6 cut(s) 109, 343, 464, 475, 582, 623
CviJI RGCY 6 cut(s) 55, 92, 169, 349, 369, 564
CviKI_1 RGCY 6 cut(s) 55, 92, 169, 349, 369, 564
CviQI GTAC 2 cut(s) 585, 620
DdeI CTNAG 3 cut(s) 312, 365, 450
DpnI GATC 7 cut(s) 126, 253, 263, 375, 472, 483, 516
DpnII GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
Ecl136II GAGCTC 1 cut(s) 349
Eco24I GRGCYC 2 cut(s) 351, 566
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 609
EcoICRI GAGCTC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 2 cut(s) 351, 566
FaeI CATG 6 cut(s) 112, 346, 467, 478, 585, 626
FatI CATG 6 cut(s) 108, 342, 463, 474, 581, 622
FauNDI CATATG 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 2 cut(s) 17, 26
FriOI GRGCYC 2 cut(s) 351, 566
Fsp4HI GCNGC 1 cut(s) 90
FspBI CTAG 1 cut(s) 189
GluI GCNGC 1 cut(s) 90
HaeIII GGCC 1 cut(s) 55
HgaI GACGC 1 cut(s) 254
Hin1II CATG 6 cut(s) 112, 346, 467, 478, 585, 626
HinfI GANTC 2 cut(s) 408, 437
Hpy188I TCNGA 3 cut(s) 261, 313, 407
Hpy188III TCNNGA 1 cut(s) 205
Hpy99I CGWCG 2 cut(s) 248, 407
HpyAV CCTTC 2 cut(s) 73, 584
HpyCH4III ACNGT 2 cut(s) 202, 574
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 3 cut(s) 8, 112, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpyF3I CTNAG 3 cut(s) 312, 365, 450
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 6 cut(s) 112, 346, 467, 478, 585, 626
Kzo9I GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
LmnI GCTCC 2 cut(s) 346, 354
LpnPI CCDG 6 cut(s) 106, 234, 283, 316, 447, 602
Lsp1109I GCAGC 1 cut(s) 101
MaeI CTAG 1 cut(s) 189
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 4 cut(s) 95, 179, 293, 382
MalI GATC 7 cut(s) 126, 253, 263, 375, 472, 483, 516
MboI GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
MboII GAAGA 3 cut(s) 88, 157, 520
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 4 cut(s) 219, 318, 351, 566
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 9 cut(s) 4, 31, 98, 206, 232, 327, 450, 455, 588
Mph1103I ATGCAT 1 cut(s) 628
MseI TTAA 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 301
MwoI GCNNNNNNNGC 1 cut(s) 313
NdeI CATATG 1 cut(s) 354
NdeII GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
NlaIII CATG 6 cut(s) 112, 346, 467, 478, 585, 626
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 2 cut(s) 293, 382
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 3 cut(s) 112, 467, 626
PaeI GCATGC 1 cut(s) 112
PctI GAATGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 437
PkrI GCNGC 1 cut(s) 91
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 2 cut(s) 53, 358
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 2 cut(s) 586, 621
RsaNI GTAC 2 cut(s) 585, 620
SacI GAGCTC 1 cut(s) 351
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 7 cut(s) 124, 251, 261, 373, 470, 481, 514
Sau96I GGNCC 2 cut(s) 53, 358
SchI GAGTC 1 cut(s) 417
SduI GDGCHC 4 cut(s) 219, 318, 351, 566
SetI ASST 7 cut(s) 36, 171, 190, 338, 351, 595, 621
SfcI CTRYAG 1 cut(s) 550
SinI GGWCC 1 cut(s) 358
SphI GCATGC 1 cut(s) 112
SspMI CTAG 1 cut(s) 189
SstI GAGCTC 1 cut(s) 351
TaaI ACNGT 2 cut(s) 202, 574
TaiI ACGT 1 cut(s) 36
TaqI TCGA 3 cut(s) 27, 123, 484
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 2 cut(s) 302, 577
TseFI GTSAC 2 cut(s) 293, 382
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 2 cut(s) 293, 382
TspDTI ATGAA 1 cut(s) 89
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 2 cut(s) 302, 577
VpaK11BI GGWCC 1 cut(s) 358
XceI RCATGY 3 cut(s) 112, 467, 626
XspI CTAG 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.