FvH4_6g16682

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003273
Physical Location & Seq
Forward (+)
10580360 .. 10582272
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16682.t3

Sequence Viewer

Length: 855 bp
ATGAGGATGGGTCAGAGCAAGTCCATCTCCACTTCTTTGTTTCTGCTGCTGCTGCTGCTGCTTCTTCAAATTTTGCACCCAACACCCATTACAGCTCAAGAAGTTGAGGACGAAAGGGAATTTGATTATCTGAAAGATAGTGAGAAAGGACCAAAGCATTGGGGAGAGCTTAGGGAAGAATGGGCAGCATGTAAAAATGGGACACTGCAATCTCCGGTAGATTTGCTTAGTGAGATCGTGGAAGTACACCCCAACAAAGGGGAGGTGAGGCTCAAGTACAGGCCTTCAAATGCAACAATCAGGAATAGAGGCCATGACATTTCGATCCAGTGGAAGCTAGGTGATGCTGGATCCATGGAGATTAATGGCACAGATTACTGGCTAGATCAATGCCACTGGCACTCACCAACTGAGCATACAATTAACGGTAAAATATTTGACCTGGAGCTTCATATGGTTCACCTCATTCCTGATGCAAAGGTCGAAAACAACATAGTTGTGGTTGCATACCTCTACAAAATTGGCAAGCCTGATAAGTTTCTCTCCGAGGTAAGCAAAGATATCAAGAGCATGATTGATGTAAAGGAAGAGAGACACAGAGGGTTGGTTGATCCAAGAAATGTGAGGAAGGGTGGCGCAAAATTTTACAAGTACAAAGGCTCACTGACTGTACCTCCTTGCACTGAAGGAGTAACTTGGATCATACACAAAAAGTTAACTACTGTATCAAGAGAGCAAATGGAGTTGCTTCGAGAAGCCGTTCATGACTCCGCAGAACTGAATGCAAGACCATTACAAGCTCTGAATCGCCGAGATGTGCACTTCCATGGCCCTGAGTCTCACATAAGGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

285

Amino Acids

32.62

Weight (kDa)

6.74

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 49 - 177 5.6e-17 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 771
AclWI GGATC 5 cut(s) 319, 345, 358, 605, 707
AcsI RAATTY 3 cut(s) 69, 119, 641
AcuI CTGAAG 1 cut(s) 705
AfaI GTAC 4 cut(s) 246, 278, 653, 672
AfiI CCNNNNNNNGG 2 cut(s) 257, 258
AgsI TTSAA 2 cut(s) 68, 288
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 5 cut(s) 95, 169, 337, 448, 800
AluI AGCT 5 cut(s) 95, 169, 337, 448, 800
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 2 cut(s) 586, 843
Alw44I GTGCAC 1 cut(s) 818
AlwI GGATC 5 cut(s) 319, 345, 358, 605, 707
AoxI GGCC 3 cut(s) 281, 310, 829
ApaLI GTGCAC 1 cut(s) 818
ApeKI GCWGC 6 cut(s) 46, 49, 52, 55, 58, 185
ApoI RAATTY 3 cut(s) 69, 119, 641
AseI ATTAAT 1 cut(s) 363
Asp700I GAANNNNTTC 1 cut(s) 759
AspLEI GCGC 1 cut(s) 638
AspS9I GGNCC 2 cut(s) 149, 830
AsuHPI GGTGA 4 cut(s) 277, 353, 396, 452
AvaII GGWCC 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 822
BaeI ACNNNNGTAYC 2 cut(s) 708, 741
BamHI GGATCC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 822
BbvI GCAGC 6 cut(s) 33, 36, 39, 42, 45, 197
BccI CCATC 1 cut(s) 32
BceAI ACGGC 1 cut(s) 743
BciT130I CCWGG 1 cut(s) 443
BcoDI GTCTC 2 cut(s) 586, 843
BfaI CTAG 2 cut(s) 338, 383
BisI GCNGC 6 cut(s) 47, 50, 53, 56, 59, 186
BlsI GCNGC 6 cut(s) 48, 51, 54, 57, 60, 187
Bme1390I CCNGG 1 cut(s) 443
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 2 cut(s) 149, 830
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 1 cut(s) 443
BmsI GCATC 2 cut(s) 334, 463
BpmI CTGGAG 1 cut(s) 464
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 81, 257
BsaJI CCNNGG 3 cut(s) 354, 546, 826
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 258
Bse1I ACTGG 3 cut(s) 328, 383, 401
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 3 cut(s) 354, 546, 826
BseGI GGATG 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 257, 258
BseMII CTCAG 2 cut(s) 402, 825
BseNI ACTGG 3 cut(s) 328, 383, 401
BseSI GKGCMC 1 cut(s) 822
BseXI GCAGC 6 cut(s) 33, 36, 39, 42, 45, 197
BshFI GGCC 3 cut(s) 283, 312, 831
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 257, 258
BsmAI GTCTC 2 cut(s) 586, 843
BsmFI GGGAC 1 cut(s) 214
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 3 cut(s) 283, 312, 831
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 6 cut(s) 234, 324, 350, 385, 610, 699
Bsp19I CCATGG 2 cut(s) 354, 826
BspACI CCGC 1 cut(s) 771
BspANI GGCC 3 cut(s) 283, 312, 831
BspCNI CTCAG 2 cut(s) 403, 826
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 1 cut(s) 352
BspPI GGATC 5 cut(s) 319, 345, 358, 605, 707
BsrI ACTGG 3 cut(s) 328, 383, 401
BssECI CCNNGG 3 cut(s) 354, 546, 826
BssMI GATC 6 cut(s) 234, 324, 350, 385, 610, 699
BssT1I CCWWGG 2 cut(s) 354, 826
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 3 cut(s) 428, 670, 724
Bst6I CTCTTC 1 cut(s) 582
BstC8I GCNNGC 1 cut(s) 527
BstDEI CTNAG 4 cut(s) 170, 227, 411, 834
BstDSI CCRYGG 2 cut(s) 354, 826
BstF5I GGATG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 638
BstKTI GATC 6 cut(s) 237, 327, 353, 388, 613, 702
BstMAI GTCTC 2 cut(s) 586, 843
BstMBI GATC 6 cut(s) 234, 324, 350, 385, 610, 699
BstMWI GCNNNNNNNGC 3 cut(s) 52, 55, 58
BstNI CCWGG 1 cut(s) 443
BstNSI RCATGY 1 cut(s) 192
BstSCI CCNGG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 6 cut(s) 33, 36, 39, 42, 45, 197
BstX2I RGATCY 1 cut(s) 350
BstXI CCANNNNNNTGG 1 cut(s) 159
BstYI RGATCY 1 cut(s) 350
BsuRI GGCC 3 cut(s) 283, 312, 831
BtgI CCRYGG 2 cut(s) 354, 826
BtsCI GGATG 1 cut(s) 12
BtsI GCAGTG 1 cut(s) 203
BtsIMutI CAGTG 5 cut(s) 203, 335, 394, 662, 681
Cac8I GCNNGC 1 cut(s) 527
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 638
Cfr13I GGNCC 2 cut(s) 149, 830
Csp6I GTAC 4 cut(s) 245, 277, 652, 671
CviAII CATG 6 cut(s) 189, 314, 355, 571, 764, 827
CviQI GTAC 4 cut(s) 245, 277, 652, 671
DdeI CTNAG 4 cut(s) 170, 227, 411, 834
DpnI GATC 6 cut(s) 236, 326, 352, 387, 612, 701
DpnII GATC 6 cut(s) 234, 324, 350, 385, 610, 699
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
Eco130I CCWWGG 2 cut(s) 354, 826
Eco147I AGGCCT 1 cut(s) 283
Eco32I GATATC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 705
EcoRII CCWGG 1 cut(s) 441
EcoRV GATATC 1 cut(s) 562
EcoT14I CCWWGG 2 cut(s) 354, 826
ErhI CCWWGG 2 cut(s) 354, 826
FaeI CATG 6 cut(s) 192, 317, 358, 574, 767, 830
FaqI GGGAC 1 cut(s) 214
FatI CATG 6 cut(s) 188, 313, 354, 570, 763, 826
FauNDI CATATG 1 cut(s) 453
Fnu4HI GCNGC 6 cut(s) 47, 50, 53, 56, 59, 186
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 6 cut(s) 47, 50, 53, 56, 59, 186
FspBI CTAG 2 cut(s) 338, 383
GlaI GCGC 1 cut(s) 637
GluI GCNGC 6 cut(s) 47, 50, 53, 56, 59, 186
GsuI CTGGAG 1 cut(s) 464
HaeIII GGCC 3 cut(s) 283, 312, 831
HapII CCGG 1 cut(s) 215
HhaI GCGC 1 cut(s) 638
Hin1II CATG 6 cut(s) 192, 317, 358, 574, 767, 830
Hin6I GCGC 1 cut(s) 636
HinP1I GCGC 1 cut(s) 636
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HinfI GANTC 3 cut(s) 767, 805, 836
HpaI GTTAAC 1 cut(s) 717
HpaII CCGG 1 cut(s) 215
HphI GGTGA 4 cut(s) 277, 353, 396, 452
Hpy166II GTNNAC 4 cut(s) 247, 460, 717, 820
Hpy188I TCNGA 4 cut(s) 15, 132, 547, 804
Hpy188III TCNNGA 7 cut(s) 98, 301, 470, 565, 729, 752, 764
Hpy8I GTNNAC 4 cut(s) 247, 460, 717, 820
HpyAV CCTTC 3 cut(s) 294, 622, 680
HpyCH4III ACNGT 3 cut(s) 428, 670, 724
HpyCH4V TGCA 8 cut(s) 76, 208, 293, 476, 506, 681, 785, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 55, 58
HpyF3I CTNAG 4 cut(s) 170, 227, 411, 834
Hsp92II CATG 6 cut(s) 192, 317, 358, 574, 767, 830
HspAI GCGC 1 cut(s) 636
KspAI GTTAAC 1 cut(s) 717
Kzo9I GATC 6 cut(s) 234, 324, 350, 385, 610, 699
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 6 cut(s) 33, 36, 39, 42, 45, 197
LweI GCATC 2 cut(s) 334, 463
MaeI CTAG 2 cut(s) 338, 383
MaeIII GTNAC 1 cut(s) 691
MalI GATC 6 cut(s) 236, 326, 352, 387, 612, 701
MboI GATC 6 cut(s) 234, 324, 350, 385, 610, 699
MboII GAAGA 3 cut(s) 56, 188, 599
MflI RGATCY 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 5 cut(s) 69, 119, 420, 519, 641
MlyI GAGTC 2 cut(s) 761, 845
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 4 cut(s) 363, 423, 716, 853
MslI CAYNNNNRTG 2 cut(s) 497, 825
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 1 cut(s) 443
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 1 cut(s) 443
MwoI GCNNNNNNNGC 3 cut(s) 52, 55, 58
NcoI CCATGG 2 cut(s) 354, 826
NdeI CATATG 1 cut(s) 453
NdeII GATC 6 cut(s) 234, 324, 350, 385, 610, 699
NlaIII CATG 6 cut(s) 192, 317, 358, 574, 767, 830
NlaIV GGNNCC 1 cut(s) 352
NmeAIII GCCGAG 1 cut(s) 836
NspI RCATGY 1 cut(s) 192
PagI TCATGA 1 cut(s) 763
PceI AGGCCT 1 cut(s) 283
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 1 cut(s) 805
PkrI GCNGC 6 cut(s) 48, 51, 54, 57, 60, 187
PleI GAGTC 2 cut(s) 761, 844
PpsI GAGTC 2 cut(s) 761, 844
PshBI ATTAAT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 441
PspGI CCWGG 1 cut(s) 441
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 2 cut(s) 149, 830
PsuI RGATCY 1 cut(s) 350
RsaI GTAC 4 cut(s) 246, 278, 653, 672
RsaNI GTAC 4 cut(s) 245, 277, 652, 671
RseI CAYNNNNRTG 2 cut(s) 497, 825
SaqAI TTAA 4 cut(s) 363, 423, 716, 853
SatI GCNGC 6 cut(s) 47, 50, 53, 56, 59, 186
Sau3AI GATC 6 cut(s) 234, 324, 350, 385, 610, 699
Sau96I GGNCC 2 cut(s) 149, 830
SchI GAGTC 2 cut(s) 761, 845
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 2 cut(s) 334, 463
SinI GGWCC 1 cut(s) 149
SmiMI CAYNNNNRTG 2 cut(s) 497, 825
SmlI CTYRAG 2 cut(s) 96, 272
SmoI CTYRAG 2 cut(s) 96, 272
Sse9I AATT 5 cut(s) 69, 119, 420, 519, 641
SseBI AGGCCT 1 cut(s) 283
SsiI CCGC 1 cut(s) 771
SspI AATATT 1 cut(s) 435
SspMI CTAG 2 cut(s) 338, 383
StuI AGGCCT 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 441
StyI CCWWGG 2 cut(s) 354, 826
TaaI ACNGT 3 cut(s) 428, 670, 724
TaqI TCGA 3 cut(s) 323, 483, 751
TasI AATT 5 cut(s) 69, 119, 420, 519, 641
TatI WGTACW 3 cut(s) 244, 276, 651
TfiI GAWTC 1 cut(s) 805
Tru1I TTAA 4 cut(s) 363, 423, 716, 853
Tru9I TTAA 4 cut(s) 363, 423, 716, 853
TscAI CASTG 5 cut(s) 210, 335, 401, 669, 688
TseI GCWGC 6 cut(s) 46, 49, 52, 55, 58, 185
TspDTI ATGAA 2 cut(s) 440, 752
TspRI CASTG 5 cut(s) 210, 335, 401, 669, 688
VneI GTGCAC 1 cut(s) 818
VpaK11BI GGWCC 1 cut(s) 149
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 3 cut(s) 69, 119, 641
XceI RCATGY 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 2 cut(s) 338, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.