FvH4_6g16690

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003273
Physical Location & Seq
Forward (+)
10594727 .. 10597193
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16690.t1

Sequence Viewer

Length: 849 bp
ATGATGATGGATCAGAGCAAGTCCATCTCCATTACTTTATTTCTGATGGTGCTTCTTCTTCAAATTCTGCAGCCGACATCCATCACAGCTCAAGAAGTTGAGGACGAAAGTGAGTTTGATTATCTGAAAGACAGTGAGAAAGGGCCAAAGCATTGGGGAGAGCTTAGGGAAGAATGGGGAGCATGTAAAACTGGGAGACTGCAATCTCCGGTAGATTTGCATAGTGAGATCGTGGAAGTACACCCCAACAATGGGGAGGTGAGACTCAAGTACAGGCCTTCATATGCAATAATCAAGAATAGAGGCCATGACATTTCGATCCAGTGGAAGCTAGGTGCAGCTGGATCCGTGGAGATTAATGGCACAGATTACTGGCTAGATCAATGCCACTGGCACTCACCATCTGAGCATACAATTAACGGTAACAGATTCGACCTCGAACTTCATATGGTTCACCGAATCCCTGGTCCAAATGTCGGAAACAACATAGTTGTGGTTGCATACCTCTACAAAACTGGCAAGCCTGATATGTTTCTCTCCCAGGTAAGCCAGGATATCAAGAGCATGATTGATGTAGAGGCAGAGAGACACAGAGGGGTGGTTGATCCAAGAGATGTGAAGAACGGTGGCGCTAAATTTTATAAGTACAAAGGCTCACTCACTGTACCTCCTTGCACTGAAGGAGTAACTTGGATCATACACAAAAAGTTAACTACTGTATCAAGAAAGCAAATGGAGTTGCTTCGAGAAGCTGTTCATGACTACGCAGAACTGAATGCAAGACCATTACAAGCTCTGAATGGCCGAGATGTGCACTTTCATGTTTCTGAGTCTCACTTAATGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

283

Amino Acids

32.16

Weight (kDa)

6.2

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 47 - 272 3e-38 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 642
AclWI GGATC 6 cut(s) 18, 313, 339, 352, 599, 701
AcoI YGGCCR 1 cut(s) 802
AcsI RAATTY 2 cut(s) 63, 635
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 4 cut(s) 240, 272, 647, 666
AfiI CCNNNNNNNGG 3 cut(s) 251, 252, 476
AgsI TTSAA 1 cut(s) 62
AjnI CCWGG 3 cut(s) 463, 540, 549
AluBI AGCT 6 cut(s) 89, 163, 331, 341, 752, 794
AluI AGCT 6 cut(s) 89, 163, 331, 341, 752, 794
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 4 cut(s) 190, 256, 580, 837
Alw44I GTGCAC 1 cut(s) 812
AlwI GGATC 6 cut(s) 18, 313, 339, 352, 599, 701
AoxI GGCC 4 cut(s) 143, 275, 304, 802
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 2 cut(s) 70, 338
ApoI RAATTY 2 cut(s) 63, 635
ArsI GACNNNNNNTTYG 2 cut(s) 451, 483
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 753
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 2 cut(s) 143, 467
AsuHPI GGTGA 3 cut(s) 271, 390, 446
AvaII GGWCC 1 cut(s) 467
BaeGI GKGCMC 1 cut(s) 816
BaeI ACNNNNGTAYC 2 cut(s) 702, 735
BamHI GGATCC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 816
BbvI GCAGC 2 cut(s) 82, 350
BccI CCATC 4 cut(s) 32, 40, 89, 409
BciT130I CCWGG 3 cut(s) 465, 542, 551
BcoDI GTCTC 4 cut(s) 190, 256, 580, 837
BfaI CTAG 2 cut(s) 332, 377
BfmI CTRYAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 633
BisI GCNGC 2 cut(s) 71, 339
BlsI GCNGC 2 cut(s) 72, 340
Bme1390I CCNGG 3 cut(s) 465, 542, 551
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 143, 467
BmiI GGNNCC 1 cut(s) 346
BmrFI CCNGG 3 cut(s) 465, 542, 551
BmrI ACTGGG 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 164
BpuEI CTTGAG 2 cut(s) 75, 251
BsaJI CCNNGG 3 cut(s) 348, 463, 540
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 252, 476
Bse1I ACTGG 5 cut(s) 196, 322, 377, 395, 520
BseBI CCWGG 3 cut(s) 465, 542, 551
BseDI CCNNGG 3 cut(s) 348, 463, 540
BseGI GGATG 1 cut(s) 77
BseLI CCNNNNNNNGG 3 cut(s) 251, 252, 476
BseMII CTCAG 2 cut(s) 396, 819
BseNI ACTGG 5 cut(s) 196, 322, 377, 395, 520
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 2 cut(s) 82, 350
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 4 cut(s) 145, 277, 306, 804
BsiHKAI GWGCWC 1 cut(s) 816
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 3 cut(s) 251, 252, 476
BsmAI GTCTC 4 cut(s) 190, 256, 580, 837
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 4 cut(s) 145, 277, 306, 804
Bsp1286I GDGCHC 1 cut(s) 816
Bsp143I GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
BspANI GGCC 4 cut(s) 145, 277, 306, 804
BspCNI CTCAG 2 cut(s) 397, 820
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 1 cut(s) 346
BspMAI CTGCAG 1 cut(s) 72
BspPI GGATC 6 cut(s) 18, 313, 339, 352, 599, 701
BsrI ACTGG 5 cut(s) 196, 322, 377, 395, 520
BssECI CCNNGG 3 cut(s) 348, 463, 540
BssMI GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
Bst2UI CCWGG 3 cut(s) 465, 542, 551
Bst4CI ACNGT 5 cut(s) 134, 422, 626, 664, 718
BstC8I GCNNGC 1 cut(s) 521
BstDEI CTNAG 3 cut(s) 164, 405, 828
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 7 cut(s) 13, 231, 321, 347, 382, 607, 696
BstMAI GTCTC 4 cut(s) 190, 256, 580, 837
BstMBI GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
BstNI CCWGG 3 cut(s) 465, 542, 551
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 3 cut(s) 463, 540, 549
BstSFI CTRYAG 1 cut(s) 68
BstSLI GKGCMC 1 cut(s) 816
BstV1I GCAGC 2 cut(s) 82, 350
BstX2I RGATCY 1 cut(s) 344
BstXI CCANNNNNNTGG 1 cut(s) 153
BstYI RGATCY 1 cut(s) 344
BsuRI GGCC 4 cut(s) 145, 277, 306, 804
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 1 cut(s) 77
BtsIMutI CAGTG 5 cut(s) 139, 329, 388, 660, 675
Cac8I GCNNGC 1 cut(s) 521
CciI TCATGA 1 cut(s) 757
CfoI GCGC 1 cut(s) 632
Cfr13I GGNCC 2 cut(s) 143, 467
Csp6I GTAC 4 cut(s) 239, 271, 646, 665
CviAII CATG 5 cut(s) 183, 308, 565, 758, 821
CviQI GTAC 4 cut(s) 239, 271, 646, 665
DdeI CTNAG 3 cut(s) 164, 405, 828
DpnI GATC 7 cut(s) 12, 230, 320, 346, 381, 606, 695
DpnII GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
EaeI YGGCCR 1 cut(s) 802
Eco147I AGGCCT 1 cut(s) 277
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 699
EcoRII CCWGG 3 cut(s) 463, 540, 549
EcoRV GATATC 1 cut(s) 556
FaeI CATG 5 cut(s) 186, 311, 568, 761, 824
FatI CATG 5 cut(s) 182, 307, 564, 757, 820
FauNDI CATATG 2 cut(s) 283, 447
Fnu4HI GCNGC 2 cut(s) 71, 339
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 71, 339
FspBI CTAG 2 cut(s) 332, 377
GlaI GCGC 1 cut(s) 631
GluI GCNGC 2 cut(s) 71, 339
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 4 cut(s) 145, 277, 306, 804
HapII CCGG 1 cut(s) 209
HhaI GCGC 1 cut(s) 632
Hin1II CATG 5 cut(s) 186, 311, 568, 761, 824
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 1 cut(s) 711
HindII GTYRAC 1 cut(s) 711
HinfI GANTC 4 cut(s) 264, 429, 459, 830
HpaI GTTAAC 1 cut(s) 711
HpaII CCGG 1 cut(s) 209
HphI GGTGA 3 cut(s) 271, 390, 446
Hpy166II GTNNAC 4 cut(s) 241, 454, 711, 814
Hpy188I TCNGA 7 cut(s) 15, 45, 126, 406, 479, 798, 829
Hpy188III TCNNGA 6 cut(s) 92, 295, 559, 723, 746, 758
Hpy8I GTNNAC 4 cut(s) 241, 454, 711, 814
HpyAV CCTTC 2 cut(s) 288, 674
HpyCH4III ACNGT 5 cut(s) 134, 422, 626, 664, 718
HpyCH4V TGCA 9 cut(s) 70, 202, 220, 287, 338, 500, 675, 779, 814
HpyF3I CTNAG 3 cut(s) 164, 405, 828
Hsp92II CATG 5 cut(s) 186, 311, 568, 761, 824
HspAI GCGC 1 cut(s) 630
KspAI GTTAAC 1 cut(s) 711
Kzo9I GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
LmnI GCTCC 1 cut(s) 179
Lsp1109I GCAGC 2 cut(s) 82, 350
MaeI CTAG 2 cut(s) 332, 377
MaeIII GTNAC 2 cut(s) 422, 685
MalI GATC 7 cut(s) 12, 230, 320, 346, 381, 606, 695
MboI GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
MboII GAAGA 4 cut(s) 47, 50, 182, 631
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 816
MluCI AATT 3 cut(s) 63, 414, 635
MlyI GAGTC 2 cut(s) 258, 839
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 8 cut(s) 94, 250, 296, 446, 515, 571, 587, 678
MroXI GAANNNNTTC 1 cut(s) 753
MseI TTAA 5 cut(s) 357, 417, 710, 839, 847
MslI CAYNNNNRTG 2 cut(s) 491, 819
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 3 cut(s) 465, 542, 551
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 3 cut(s) 465, 542, 551
NdeI CATATG 2 cut(s) 283, 447
NdeII GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
NlaIII CATG 5 cut(s) 186, 311, 568, 761, 824
NlaIV GGNNCC 1 cut(s) 346
NmeAIII GCCGAG 1 cut(s) 830
NspI RCATGY 1 cut(s) 186
PagI TCATGA 1 cut(s) 757
PceI AGGCCT 1 cut(s) 277
PctI GAATGC 1 cut(s) 781
PdmI GAANNNNTTC 1 cut(s) 753
PfeI GAWTC 2 cut(s) 429, 459
PkrI GCNGC 2 cut(s) 72, 340
PleI GAGTC 2 cut(s) 258, 838
PpsI GAGTC 2 cut(s) 258, 838
PshBI ATTAAT 1 cut(s) 357
PsiI TTATAA 1 cut(s) 642
Psp6I CCWGG 3 cut(s) 463, 540, 549
PspGI CCWGG 3 cut(s) 463, 540, 549
PspN4I GGNNCC 1 cut(s) 346
PspPI GGNCC 2 cut(s) 143, 467
PstI CTGCAG 1 cut(s) 72
PsuI RGATCY 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 4 cut(s) 240, 272, 647, 666
RsaNI GTAC 4 cut(s) 239, 271, 646, 665
RseI CAYNNNNRTG 2 cut(s) 491, 819
SaqAI TTAA 5 cut(s) 357, 417, 710, 839, 847
SatI GCNGC 2 cut(s) 71, 339
Sau3AI GATC 7 cut(s) 10, 228, 318, 344, 379, 604, 693
Sau96I GGNCC 2 cut(s) 143, 467
SchI GAGTC 2 cut(s) 258, 839
ScrFI CCNGG 3 cut(s) 465, 542, 551
SduI GDGCHC 1 cut(s) 816
SfcI CTRYAG 1 cut(s) 68
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 491, 819
SmlI CTYRAG 2 cut(s) 90, 266
SmoI CTYRAG 2 cut(s) 90, 266
Sse9I AATT 3 cut(s) 63, 414, 635
SseBI AGGCCT 1 cut(s) 277
SspMI CTAG 2 cut(s) 332, 377
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 3 cut(s) 463, 540, 549
TaaI ACNGT 5 cut(s) 134, 422, 626, 664, 718
TaqI TCGA 4 cut(s) 317, 432, 438, 745
TasI AATT 3 cut(s) 63, 414, 635
TatI WGTACW 3 cut(s) 238, 270, 645
TfiI GAWTC 2 cut(s) 429, 459
Tru1I TTAA 5 cut(s) 357, 417, 710, 839, 847
Tru9I TTAA 5 cut(s) 357, 417, 710, 839, 847
TscAI CASTG 5 cut(s) 139, 329, 395, 667, 682
TseI GCWGC 2 cut(s) 70, 338
TspDTI ATGAA 4 cut(s) 270, 434, 746, 809
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 5 cut(s) 139, 329, 395, 667, 682
VneI GTGCAC 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 467
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 2 cut(s) 63, 635
XceI RCATGY 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 753
XspI CTAG 2 cut(s) 332, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.