MD00G1067900.v1.1

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003273
Physical Location & Seq
Reverse (-)
13258267 .. 13260209
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1067900.v1.1.491

Sequence Viewer

Length: 840 bp
ATGAAGAAGTCGCATCAAAGCAAACCCATATACTTTTTCTGTTTAGTATTCTTTTTATGTTCCGCAACATCGATCACAGCTCAAGAAGTTGAAGATGAACGAGAGTTCGATTATCTTCAGGGAAGTGGAAAGGGACCGAAGCAGTGGGGAGCGATTAAGAAAGAATGGTCAGCCTGTAAGAATGGAGGCATGCAATCTCCTATTGATTTATCGAGTCACAGGGTCACGTTAGTCCCAAACTTGGGCAAGCTCAACATCTCTTACAATCCTTGTAATGCTACCGTAAAGAATAGAGGCCACGATATTTCGATTTACTGGGAGGGCAACGCTGGATCAATTCATATAAATGGTACTCAGTATTTGCTAAAACAATCTCACTGGCACTCCCCTTCCGAGCACTCCGTCAATGGCAGGAGGTACGACATGGAGCTCCACATGGTTCATCTAAGCTCCGATCCCAATGTCACAAACAAGATCGCTGTTGTTGGCGTCCTATACAAGATTGGTCGCCCTGACTCTTTCCTTTCTAAGTTGACAGGGCATGTAAAGTCAATGATGGATCAGACGGAAGAGAGAAACATAGGGACAATTGATCCCCGAGAAATTAAAATCCGTAAGAAAAACTATTACAGATACATGGGCTCACTCACTGTTCCTCCTTGCACTGAAGGCGTTATTTGGACCATCAATAAAAAGATAAGAACAGTCTCAAGGGATCAAGTAAAGCTATTTCGAGTCGCTGTGCATGATTATGCAGAGATGAATGCAAGGCCAGTACAACCACTTAATCTTCGAGAGATCCAGGTCTTTGACAGAAGAACAGGGAGCACAAATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

280

Amino Acids

31.83

Weight (kDa)

9.47

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 45 - 268 6.1e-46 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 63
AclWI GGATC 6 cut(s) 340, 449, 567, 587, 723, 793
AcuI CTGAAG 2 cut(s) 101, 687
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 3 cut(s) 352, 419, 777
AfiI CCNNNNNNNGG 2 cut(s) 241, 242
AgsI TTSAA 1 cut(s) 92
AjnI CCWGG 1 cut(s) 801
AluBI AGCT 5 cut(s) 80, 250, 430, 450, 727
AluI AGCT 5 cut(s) 80, 250, 430, 450, 727
Alw21I GWGCWC 3 cut(s) 399, 432, 830
Alw26I GTCTC 1 cut(s) 712
AlwI GGATC 6 cut(s) 340, 449, 567, 587, 723, 793
Ama87I CYCGRG 1 cut(s) 597
AoxI GGCC 2 cut(s) 295, 770
AspS9I GGNCC 2 cut(s) 134, 681
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 2 cut(s) 134, 681
BanII GRGCYC 2 cut(s) 432, 644
Bbv12I GWGCWC 3 cut(s) 399, 432, 830
BccI CCATC 2 cut(s) 550, 692
BciT130I CCWGG 1 cut(s) 803
BcoDI GTCTC 1 cut(s) 712
Bme1390I CCNGG 1 cut(s) 803
Bme18I GGWCC 2 cut(s) 134, 681
BmeT110I CYCGRG 1 cut(s) 597
BmgT120I GGNCC 2 cut(s) 134, 681
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 803
BmrI ACTGGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 22
BmuI ACTGGG 1 cut(s) 325
BpuEI CTTGAG 2 cut(s) 66, 694
Bsa29I ATCGAT 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 489
BsaXI ACNNNNNCTCC 4 cut(s) 368, 398, 640, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 242
Bse1I ACTGG 3 cut(s) 320, 383, 773
BseBI CCWGG 1 cut(s) 803
BseCI ATCGAT 1 cut(s) 71
BseLI CCNNNNNNNGG 2 cut(s) 241, 242
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 3 cut(s) 320, 383, 773
BshFI GGCC 2 cut(s) 297, 772
BshVI ATCGAT 1 cut(s) 71
BsiHKAI GWGCWC 3 cut(s) 399, 432, 830
BsiHKCI CYCGRG 1 cut(s) 597
BslFI GGGAC 3 cut(s) 147, 218, 598
BslI CCNNNNNNNGG 2 cut(s) 241, 242
BsmAI GTCTC 1 cut(s) 712
BsmFI GGGAC 3 cut(s) 147, 218, 598
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 2 cut(s) 297, 772
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 4 cut(s) 399, 432, 644, 830
Bsp143I GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
BspACI CCGC 1 cut(s) 63
BspANI GGCC 2 cut(s) 297, 772
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 6 cut(s) 340, 449, 567, 587, 723, 793
BsrI ACTGG 3 cut(s) 320, 383, 773
BssMI GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
BssNI GRCGYC 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 3 cut(s) 283, 652, 706
Bst6I CTCTTC 1 cut(s) 564
BstACI GRCGYC 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 191, 248
BstDEI CTNAG 3 cut(s) 354, 446, 528
BstKTI GATC 8 cut(s) 75, 335, 457, 477, 562, 595, 718, 801
BstMAI GTCTC 1 cut(s) 712
BstMBI GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
BstMWI GCNNNNNNNGC 1 cut(s) 669
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 2 cut(s) 193, 545
BstSCI CCNGG 1 cut(s) 801
BstX2I RGATCY 1 cut(s) 798
BstYI RGATCY 1 cut(s) 798
Bsu15I ATCGAT 1 cut(s) 71
BsuRI GGCC 2 cut(s) 297, 772
BsuTUI ATCGAT 1 cut(s) 71
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 4 cut(s) 149, 376, 648, 663
Cac8I GCNNGC 2 cut(s) 191, 248
Cfr13I GGNCC 2 cut(s) 134, 681
ClaI ATCGAT 1 cut(s) 71
CseI GACGC 1 cut(s) 478
Csp6I GTAC 3 cut(s) 351, 418, 776
CviAII CATG 6 cut(s) 190, 424, 436, 542, 637, 746
CviJI RGCY 9 cut(s) 80, 173, 250, 297, 430, 450, 642, 727, 772
CviKI_1 RGCY 9 cut(s) 80, 173, 250, 297, 430, 450, 642, 727, 772
CviQI GTAC 3 cut(s) 351, 418, 776
DdeI CTNAG 3 cut(s) 354, 446, 528
DpnI GATC 8 cut(s) 74, 334, 456, 476, 561, 594, 717, 800
DpnII GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 2 cut(s) 432, 644
Eco47I GGWCC 2 cut(s) 134, 681
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 2 cut(s) 101, 687
Eco88I CYCGRG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 801
EcoT38I GRGCYC 2 cut(s) 432, 644
FaeI CATG 6 cut(s) 193, 427, 439, 545, 640, 749
FaqI GGGAC 3 cut(s) 147, 218, 598
FatI CATG 6 cut(s) 189, 423, 435, 541, 636, 745
FriOI GRGCYC 2 cut(s) 432, 644
HaeIII GGCC 2 cut(s) 297, 772
HgaI GACGC 1 cut(s) 478
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 6 cut(s) 193, 427, 439, 545, 640, 749
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HinfI GANTC 3 cut(s) 214, 515, 735
Hpy166II GTNNAC 1 cut(s) 534
Hpy188I TCNGA 3 cut(s) 394, 454, 564
Hpy188III TCNNGA 2 cut(s) 83, 794
Hpy8I GTNNAC 1 cut(s) 534
HpyAV CCTTC 2 cut(s) 399, 662
HpyCH4III ACNGT 3 cut(s) 283, 652, 706
HpyCH4IV ACGT 1 cut(s) 227
HpyCH4V TGCA 5 cut(s) 193, 663, 745, 755, 767
HpyF10VI GCNNNNNNNGC 1 cut(s) 669
HpyF3I CTNAG 3 cut(s) 354, 446, 528
HpySE526I ACGT 1 cut(s) 227
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 6 cut(s) 193, 427, 439, 545, 640, 749
Kzo9I GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
LmnI GCTCC 5 cut(s) 149, 427, 435, 455, 825
LweI GCATC 1 cut(s) 22
MaeII ACGT 1 cut(s) 227
MaeIII GTNAC 3 cut(s) 215, 223, 463
MalI GATC 8 cut(s) 74, 334, 456, 476, 561, 594, 717, 800
MboI GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
MboII GAAGA 6 cut(s) 16, 104, 107, 581, 782, 828
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 1 cut(s) 798
MhlI GDGCHC 4 cut(s) 399, 432, 644, 830
MluCI AATT 3 cut(s) 336, 588, 603
MlyI GAGTC 3 cut(s) 223, 509, 744
MnlI CCTC 5 cut(s) 179, 287, 313, 408, 666
MseI TTAA 3 cut(s) 156, 606, 786
MslI CAYNNNNRTG 2 cut(s) 345, 750
MspR9I CCNGG 1 cut(s) 803
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 1 cut(s) 669
NdeII GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
NlaIII CATG 6 cut(s) 193, 427, 439, 545, 640, 749
NlaIV GGNNCC 1 cut(s) 135
NmuCI GTSAC 3 cut(s) 215, 223, 463
NspI RCATGY 2 cut(s) 193, 545
PaeI GCATGC 1 cut(s) 193
PctI GAATGC 1 cut(s) 769
PleI GAGTC 3 cut(s) 222, 509, 743
PpsI GAGTC 3 cut(s) 222, 509, 743
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 2 cut(s) 134, 681
PsuI RGATCY 1 cut(s) 798
RsaI GTAC 3 cut(s) 352, 419, 777
RsaNI GTAC 3 cut(s) 351, 418, 776
RseI CAYNNNNRTG 2 cut(s) 345, 750
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 156, 606, 786
Sau3AI GATC 8 cut(s) 72, 332, 454, 474, 559, 592, 715, 798
Sau96I GGNCC 2 cut(s) 134, 681
SchI GAGTC 3 cut(s) 223, 509, 744
ScrFI CCNGG 1 cut(s) 803
SduI GDGCHC 4 cut(s) 399, 432, 644, 830
SetI ASST 8 cut(s) 82, 230, 252, 419, 432, 452, 729, 807
SfaNI GCATC 1 cut(s) 22
SinI GGWCC 2 cut(s) 134, 681
SmiMI CAYNNNNRTG 2 cut(s) 345, 750
SmlI CTYRAG 2 cut(s) 81, 709
SmoI CTYRAG 2 cut(s) 81, 709
SphI GCATGC 1 cut(s) 193
Sse9I AATT 3 cut(s) 336, 588, 603
SsiI CCGC 1 cut(s) 63
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 801
TaaI ACNGT 3 cut(s) 283, 652, 706
TaiI ACGT 1 cut(s) 230
TaqI TCGA 6 cut(s) 71, 108, 212, 308, 733, 793
TaqII GACCGA 1 cut(s) 151
TasI AATT 3 cut(s) 336, 588, 603
TatI WGTACW 1 cut(s) 775
Tru1I TTAA 3 cut(s) 156, 606, 786
Tru9I TTAA 3 cut(s) 156, 606, 786
TscAI CASTG 4 cut(s) 149, 383, 655, 670
TseFI GTSAC 3 cut(s) 215, 223, 463
Tsp45I GTSAC 3 cut(s) 215, 223, 463
TspDTI ATGAA 5 cut(s) 17, 111, 329, 431, 776
TspGWI ACGGA 3 cut(s) 391, 581, 602
TspRI CASTG 4 cut(s) 149, 383, 655, 670
VpaK11BI GGWCC 2 cut(s) 134, 681
XceI RCATGY 2 cut(s) 193, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.