FvH4_5g15330

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001877
Physical Location & Seq
Reverse (-)
8684539 .. 8687346
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15330.t1

Sequence Viewer

Length: 459 bp
ATGTCGACTCCTGCAAGGAAGAGGCTGATGAGAGACTTCAAGAGGCTGCAACAGGACCCGCCTGCAGGGATTAGTGGGGCACCCCAAGACAACAATATAATGCTGTGGAATGCCGTTATATTTGGGCCTGATGATACCCCTTGGGATGGAGGCACGTTCAAGTTGACTCTTCAGTTTACTGAAGATTATCCAAACAAACCACCAACTGTGCGATTTGTATCTCGGATGTTTCATCCAAATATCTATGCCGATGGAAGTATCTGTTTAGATATTTTGCAAAATCAGTGGAGTCCTATCTATGATGTGGCAGCTATACTTACGTCTATCCAGTCACTACTATGTGATCCGAACCCGAATTCTCCTGCAAATTCAGAAGCTGCTCGCATGTTTAGCGAGAACAAGCGAGAGTACAATCGAAGAGTTAGGGAAATCGTGGAACAGAGTTGGACAGCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

153

Amino Acids

17.35

Weight (kDa)

5.4

Isoelectric Point (pI)

73.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 8 - 144 5.7e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 59
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 2 cut(s) 355, 367
AcuI CTGAAG 2 cut(s) 155, 201
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 65, 146
AgsI TTSAA 2 cut(s) 40, 160
AluBI AGCT 2 cut(s) 311, 377
AluI AGCT 2 cut(s) 311, 377
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 377
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 3 cut(s) 46, 308, 377
ApoI RAATTY 2 cut(s) 355, 367
AspS9I GGNCC 2 cut(s) 55, 125
AvaII GGWCC 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 82
BanI GGYRCC 1 cut(s) 79
BbvI GCAGC 3 cut(s) 33, 320, 364
BccI CCATC 2 cut(s) 140, 245
BceAI ACGGC 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 3 cut(s) 47, 309, 378
BlsI GCNGC 3 cut(s) 48, 310, 379
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 2 cut(s) 55, 125
BmiI GGNNCC 2 cut(s) 57, 81
BsaBI GATNNNNATC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 146
Bse1I ACTGG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 3 cut(s) 151, 231, 232
BseJI GATNNNNATC 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 65, 146
BseNI ACTGG 1 cut(s) 328
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 3 cut(s) 33, 320, 364
BshFI GGCC 1 cut(s) 127
BshNI GGYRCC 1 cut(s) 79
BslI CCNNNNNNNGG 2 cut(s) 65, 146
BsmAI GTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 1 cut(s) 343
BspACI CCGC 1 cut(s) 59
BspANI GGCC 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 57, 81
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 79
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 3 cut(s) 14, 174, 412
BstC8I GCNNGC 2 cut(s) 63, 382
BstF5I GGATG 3 cut(s) 151, 231, 232
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 388
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 82
BstV1I GCAGC 3 cut(s) 33, 320, 364
BsuRI GGCC 1 cut(s) 127
BtsCI GGATG 3 cut(s) 151, 231, 232
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 2 cut(s) 63, 382
CaiI CAGNNNCTG 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 55, 125
Csp6I GTAC 1 cut(s) 409
CspCI CAANNNNNGTGG 2 cut(s) 266, 301
CviAII CATG 1 cut(s) 385
CviJI RGCY 5 cut(s) 25, 46, 127, 311, 377
CviKI_1 RGCY 5 cut(s) 25, 46, 127, 311, 377
CviQI GTAC 1 cut(s) 409
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
Eam1104I CTCTTC 3 cut(s) 14, 174, 412
EarI CTCTTC 3 cut(s) 14, 174, 412
Eco130I CCWWGG 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 155, 201
EcoO109I RGGNCCY 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 1 cut(s) 388
FaiI YATR 7 cut(s) 98, 119, 246, 300, 314, 340, 386
FatI CATG 1 cut(s) 384
FauI CCCGC 1 cut(s) 66
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 47, 309, 378
FokI GGATG 3 cut(s) 158, 219, 238
Fsp4HI GCNGC 3 cut(s) 47, 309, 378
GluI GCNGC 3 cut(s) 47, 309, 378
HaeIII GGCC 1 cut(s) 127
Hin1II CATG 1 cut(s) 388
HincII GTYRAC 2 cut(s) 6, 165
HindII GTYRAC 2 cut(s) 6, 165
HinfI GANTC 3 cut(s) 7, 166, 289
Hpy166II GTNNAC 3 cut(s) 6, 165, 177
Hpy188I TCNGA 3 cut(s) 225, 348, 373
Hpy188III TCNNGA 1 cut(s) 40
Hpy8I GTNNAC 3 cut(s) 6, 165, 177
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 2 cut(s) 155, 320
HpyCH4V TGCA 5 cut(s) 14, 49, 65, 277, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 390
HpySE526I ACGT 2 cut(s) 155, 320
Hsp92II CATG 1 cut(s) 388
Kzo9I GATC 1 cut(s) 343
LpnPI CCDG 7 cut(s) 24, 38, 51, 75, 141, 341, 375
Lsp1109I GCAGC 3 cut(s) 33, 320, 364
MaeII ACGT 2 cut(s) 155, 320
MaeIII GTNAC 1 cut(s) 330
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 4 cut(s) 31, 161, 194, 429
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 2 cut(s) 355, 367
MlyI GAGTC 2 cut(s) 160, 298
MmeI TCCRAC 1 cut(s) 425
MnlI CCTC 3 cut(s) 15, 36, 143
MslI CAYNNNNRTG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 115
MwoI GCNNNNNNNGC 1 cut(s) 390
NdeII GATC 1 cut(s) 343
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 2 cut(s) 57, 81
NmuCI GTSAC 1 cut(s) 330
NspI RCATGY 1 cut(s) 388
PctI GAATGC 1 cut(s) 115
PkrI GCNGC 3 cut(s) 48, 310, 379
PleI GAGTC 2 cut(s) 160, 297
PpsI GAGTC 2 cut(s) 160, 297
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 57, 81
PspPI GGNCC 2 cut(s) 55, 125
PspPPI RGGWCCY 1 cut(s) 55
PstI CTGCAG 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 337
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 3 cut(s) 47, 309, 378
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 2 cut(s) 55, 125
SbfI CCTGCAGG 1 cut(s) 67
SchI GAGTC 2 cut(s) 160, 298
SdaI CCTGCAGG 1 cut(s) 67
SduI GDGCHC 1 cut(s) 82
SetI ASST 4 cut(s) 158, 313, 323, 379
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 337
Sse8387I CCTGCAGG 1 cut(s) 67
Sse9I AATT 2 cut(s) 355, 367
SsiI CCGC 1 cut(s) 59
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 2 cut(s) 158, 323
TaqI TCGA 2 cut(s) 5, 415
TasI AATT 2 cut(s) 355, 367
TatI WGTACW 1 cut(s) 408
TscAI CASTG 1 cut(s) 290
TseFI GTSAC 1 cut(s) 330
TseI GCWGC 3 cut(s) 46, 308, 377
Tsp45I GTSAC 1 cut(s) 330
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 2 cut(s) 355, 367
XceI RCATGY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.