MD16G1077400.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001877
Physical Location & Seq
Reverse (-)
5413051 .. 5416067
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1077400.v1.1.491

Sequence Viewer

Length: 432 bp
ATGAGGGATTTCAAGAGGTTGCAGCAGGATCCCCCTGCGGGTATAAGTGGTGCTCCGCAAGACAACAATATTATGCTTTGGAATGCTGTTATATTTGGGCCTGATGACACCCCATGGGATGGAGGTACGTTTAAGTTGACACTCCAATTCACAGAGGACTATCCAAATAAGCCACCAACAGTGCGTTTTGTTTCTCGAATGTTTCATCCAAATATTTATGCGGATGGAAGCATTTGTTTGGACATCCTACAGAATCAGTGGAGCCCTATCTATGATGTAGCAGCTATACTTACCTCTATTCAGTCTTTGCTGTGTGATCCAAACCCAAACTCTCCAGCAAATTCAGAAGCTGCACGGATGTTTAGCGAGAACAAGCGTGAATACAATAGGAGAGTCCGCGAGATTGTTGAGCAGAGTTGGACTGCCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

144

Amino Acids

16.31

Weight (kDa)

4.79

Isoelectric Point (pI)

70.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 1 - 135 5.1e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 119
AccII CGCG 1 cut(s) 399
AciI CCGC 4 cut(s) 38, 56, 221, 397
AclWI GGATC 3 cut(s) 23, 36, 311
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 2 cut(s) 38, 119
AgsI TTSAA 1 cut(s) 13
AluBI AGCT 2 cut(s) 284, 350
AluI AGCT 2 cut(s) 284, 350
Alw21I GWGCWC 1 cut(s) 55
AlwI GGATC 3 cut(s) 23, 36, 311
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 3 cut(s) 22, 281, 350
ApoI RAATTY 1 cut(s) 340
AspS9I GGNCC 1 cut(s) 98
BamHI GGATCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 55
BbvI GCAGC 3 cut(s) 34, 293, 337
BccI CCATC 2 cut(s) 113, 218
BfmI CTRYAG 1 cut(s) 248
BisI GCNGC 3 cut(s) 23, 282, 351
BlsI GCNGC 3 cut(s) 24, 283, 352
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 2 cut(s) 30, 263
BpmI CTGGAG 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 119
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 5 cut(s) 124, 205, 229, 243, 363
BseLI CCNNNNNNNGG 2 cut(s) 38, 119
BseXI GCAGC 3 cut(s) 34, 293, 337
BsgI GTGCAG 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 399
BshFI GGCC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 55
BslI CCNNNNNNNGG 2 cut(s) 38, 119
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 1 cut(s) 100
Bsp1286I GDGCHC 2 cut(s) 55, 266
Bsp143I GATC 2 cut(s) 28, 316
Bsp19I CCATGG 1 cut(s) 113
BspACI CCGC 4 cut(s) 38, 56, 221, 397
BspANI GGCC 1 cut(s) 100
BspFNI CGCG 1 cut(s) 399
BspLI GGNNCC 2 cut(s) 30, 263
BspPI GGATC 3 cut(s) 23, 36, 311
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 2 cut(s) 28, 316
BssT1I CCWWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 181
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 5 cut(s) 124, 205, 229, 243, 363
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 2 cut(s) 31, 319
BstMBI GATC 2 cut(s) 28, 316
BstSFI CTRYAG 1 cut(s) 248
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 3 cut(s) 34, 293, 337
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 1 cut(s) 100
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 5 cut(s) 124, 205, 229, 243, 363
BtsIMutI CAGTG 2 cut(s) 186, 263
CaiI CAGNNNCTG 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 126
CviAII CATG 1 cut(s) 114
CviJI RGCY 5 cut(s) 100, 172, 264, 284, 350
CviKI_1 RGCY 5 cut(s) 100, 172, 264, 284, 350
CviQI GTAC 1 cut(s) 126
DpnI GATC 2 cut(s) 30, 318
DpnII GATC 2 cut(s) 28, 316
Eco130I CCWWGG 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 1 cut(s) 117
FaiI YATR 7 cut(s) 44, 74, 92, 115, 219, 273, 287
FatI CATG 1 cut(s) 113
FauI CCCGC 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 23, 282, 351
FokI GGATG 5 cut(s) 131, 192, 230, 236, 370
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 3 cut(s) 23, 282, 351
GluI GCNGC 3 cut(s) 23, 282, 351
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 1 cut(s) 100
Hin1II CATG 1 cut(s) 117
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 2 cut(s) 253, 393
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 2 cut(s) 13, 195
Hpy8I GTNNAC 1 cut(s) 138
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 2 cut(s) 22, 353
HpySE526I ACGT 1 cut(s) 128
Hsp92II CATG 1 cut(s) 117
Kzo9I GATC 2 cut(s) 28, 316
LmnI GCTCC 2 cut(s) 58, 261
LpnPI CCDG 4 cut(s) 11, 48, 114, 348
Lsp1109I GCAGC 3 cut(s) 34, 293, 337
MaeII ACGT 1 cut(s) 128
MalI GATC 2 cut(s) 30, 318
MboI GATC 2 cut(s) 28, 316
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 2 cut(s) 55, 266
MluCI AATT 2 cut(s) 146, 340
MlyI GAGTC 1 cut(s) 402
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 4 cut(s) 9, 116, 148, 304
MseI TTAA 2 cut(s) 132, 430
Mva1269I GAATGC 1 cut(s) 88
MvnI CGCG 1 cut(s) 399
NcoI CCATGG 1 cut(s) 113
NdeII GATC 2 cut(s) 28, 316
NlaIII CATG 1 cut(s) 117
NlaIV GGNNCC 2 cut(s) 30, 263
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 253
PflMI CCANNNNNTGG 1 cut(s) 119
PkrI GCNGC 3 cut(s) 24, 283, 352
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 30, 263
PspPI GGNCC 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 28
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
SaqAI TTAA 2 cut(s) 132, 430
SatI GCNGC 3 cut(s) 23, 282, 351
Sau3AI GATC 2 cut(s) 28, 316
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 1 cut(s) 402
SduI GDGCHC 2 cut(s) 55, 266
SetI ASST 6 cut(s) 20, 127, 131, 286, 296, 352
SfcI CTRYAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 146, 340
SsiI CCGC 4 cut(s) 38, 56, 221, 397
SspI AATATT 2 cut(s) 70, 214
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 131
TaqI TCGA 1 cut(s) 196
TasI AATT 2 cut(s) 146, 340
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 132, 430
Tru9I TTAA 2 cut(s) 132, 430
TscAI CASTG 2 cut(s) 186, 263
TseI GCWGC 3 cut(s) 22, 281, 350
TspDTI ATGAA 1 cut(s) 194
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 2 cut(s) 186, 263
Van91I CCANNNNNTGG 1 cut(s) 119
XapI RAATTY 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.