RchiOBHm_Chr3g0450771

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001877
Physical Location & Seq
Forward (+)
2170122 .. 2171911
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41808

Sequence Viewer

Length: 531 bp
ATGACCCCTTCATACTTGGACAGGCTGCAGAAAAAAGTGTCGGATGACCCACCCCCGGGCGCAGATTATACTCTTGCAAGGTATAGGTTGTGTAAAGAGTTGAAGAGGCTGCTACAAGACCCACCTGCAGGGATCGGTGTGGTTCCCCCGGACTTCTACAATTGCGATATAATGTTGTGGAATGTTGTTATAGCTCCTCATTCCTTGGATTCTCCTTGGGAGGGAGGCGCATGCACATTTAAGTTGACTCTTCAGTTCAGTGAGGATTATCCGCTCAAACCATCAATGGTGCGATTTGTTTCTCAAATGTTTCATCCAAACATTTCTGCAGATGGAATTATTGTGTTGAACACCAAGAATCTGCAAAGACTCCTAACACACACCATTCACTTACTGCAGTCCCTAATATGTCATCCCAACCCGAAGTGTCCTGCAAATTCAGAAGCTTTCTACATGTATTTCAGTGAGAACAGACTCGAGTACAACCGAAGAGTGAGGATGATTGTGGATCAGAGTTGTATGGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

176

Amino Acids

20.18

Weight (kDa)

6.94

Isoelectric Point (pI)

64.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 29 - 167 2e-24 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
Acc36I ACCTGC 1 cut(s) 133
AccBSI CCGCTC 1 cut(s) 274
AciI CCGC 1 cut(s) 272
AclWI GGATC 2 cut(s) 140, 516
AcsI RAATTY 1 cut(s) 436
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
AflIII ACRYGT 1 cut(s) 453
AgsI TTSAA 2 cut(s) 103, 349
AluBI AGCT 2 cut(s) 194, 446
AluI AGCT 2 cut(s) 194, 446
AlwI GGATC 2 cut(s) 140, 516
Ama87I CYCGRG 2 cut(s) 55, 476
ApeKI GCWGC 2 cut(s) 25, 109
ApoI RAATTY 1 cut(s) 436
AspLEI GCGC 2 cut(s) 62, 230
AsuC2I CCSGG 3 cut(s) 56, 57, 149
AvaI CYCGRG 2 cut(s) 55, 476
BbvI GCAGC 2 cut(s) 12, 96
BccI CCATC 2 cut(s) 289, 326
BcnI CCSGG 3 cut(s) 56, 57, 149
BfaI CTAG 1 cut(s) 529
BfmI CTRYAG 4 cut(s) 26, 126, 327, 395
BfuAI ACCTGC 1 cut(s) 133
BisI GCNGC 2 cut(s) 26, 110
BlsI GCNGC 2 cut(s) 27, 111
Bme1390I CCNGG 3 cut(s) 56, 57, 149
BmeT110I CYCGRG 2 cut(s) 55, 476
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 3 cut(s) 56, 57, 149
BpuMI CCSGG 3 cut(s) 56, 57, 149
BsaJI CCNNGG 5 cut(s) 54, 55, 147, 204, 215
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
BseDI CCNNGG 5 cut(s) 54, 55, 147, 204, 215
BseGI GGATG 4 cut(s) 49, 313, 412, 504
BseLI CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 2 cut(s) 12, 96
BsiHKCI CYCGRG 2 cut(s) 55, 476
BsiSI CCGG 2 cut(s) 56, 149
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
BsmFI GGGAC 1 cut(s) 385
BsoBI CYCGRG 2 cut(s) 55, 476
Bsp143I GATC 2 cut(s) 132, 508
BspACI CCGC 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 144
BspMAI CTGCAG 4 cut(s) 30, 130, 331, 399
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 2 cut(s) 140, 516
BsrBI CCGCTC 1 cut(s) 274
BssECI CCNNGG 5 cut(s) 54, 55, 147, 204, 215
BssMI GATC 2 cut(s) 132, 508
BssT1I CCWWGG 2 cut(s) 204, 215
Bst6I CTCTTC 3 cut(s) 98, 255, 484
BstC8I GCNNGC 1 cut(s) 232
BstF5I GGATG 4 cut(s) 49, 313, 412, 504
BstHHI GCGC 2 cut(s) 62, 230
BstKTI GATC 2 cut(s) 135, 511
BstMBI GATC 2 cut(s) 132, 508
BstNSI RCATGY 2 cut(s) 234, 457
BstSCI CCNGG 3 cut(s) 54, 55, 147
BstSFI CTRYAG 4 cut(s) 26, 126, 327, 395
BstV1I GCAGC 2 cut(s) 12, 96
BtsCI GGATG 4 cut(s) 49, 313, 412, 504
BtsIMutI CAGTG 2 cut(s) 265, 469
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 1 cut(s) 232
CfoI GCGC 2 cut(s) 62, 230
Cfr9I CCCGGG 1 cut(s) 55
Csp6I GTAC 1 cut(s) 481
CviAII CATG 2 cut(s) 231, 454
CviJI RGCY 4 cut(s) 25, 109, 194, 446
CviKI_1 RGCY 4 cut(s) 25, 109, 194, 446
CviQI GTAC 1 cut(s) 481
DpnI GATC 2 cut(s) 134, 510
DpnII GATC 2 cut(s) 132, 508
Eam1104I CTCTTC 3 cut(s) 98, 255, 484
EarI CTCTTC 3 cut(s) 98, 255, 484
Eco130I CCWWGG 2 cut(s) 204, 215
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 2 cut(s) 55, 476
EcoT14I CCWWGG 2 cut(s) 204, 215
ErhI CCWWGG 2 cut(s) 204, 215
FaeI CATG 2 cut(s) 234, 457
FaiI YATR 9 cut(s) 13, 69, 84, 170, 191, 232, 409, 455, 521
FaqI GGGAC 1 cut(s) 385
FatI CATG 2 cut(s) 230, 453
Fnu4HI GCNGC 2 cut(s) 26, 110
FokI GGATG 4 cut(s) 56, 300, 399, 511
Fsp4HI GCNGC 2 cut(s) 26, 110
FspBI CTAG 1 cut(s) 529
GlaI GCGC 2 cut(s) 61, 229
GluI GCNGC 2 cut(s) 26, 110
HapII CCGG 2 cut(s) 56, 149
HhaI GCGC 2 cut(s) 62, 230
Hin1II CATG 2 cut(s) 234, 457
Hin6I GCGC 2 cut(s) 60, 228
HinP1I GCGC 2 cut(s) 60, 228
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 5 cut(s) 209, 247, 358, 369, 474
HpaII CCGG 2 cut(s) 56, 149
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 3 cut(s) 43, 442, 513
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 1 cut(s) 18
HpyCH4V TGCA 8 cut(s) 28, 77, 128, 234, 329, 364, 397, 434
Hsp92II CATG 2 cut(s) 234, 457
HspAI GCGC 2 cut(s) 60, 228
Kzo9I GATC 2 cut(s) 132, 508
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 6 cut(s) 7, 69, 114, 138, 162, 444
Lsp1109I GCAGC 2 cut(s) 12, 96
MaeI CTAG 1 cut(s) 529
MalI GATC 2 cut(s) 134, 510
MbiI CCGCTC 1 cut(s) 274
MboI GATC 2 cut(s) 132, 508
MboII GAAGA 3 cut(s) 115, 242, 501
MfeI CAATTG 1 cut(s) 160
MluCI AATT 3 cut(s) 160, 336, 436
MlyI GAGTC 3 cut(s) 241, 363, 468
MmeI TCCRAC 1 cut(s) 21
MnlI CCTC 6 cut(s) 99, 207, 214, 218, 256, 489
MseI TTAA 1 cut(s) 240
MspI CCGG 2 cut(s) 56, 149
MspR9I CCNGG 3 cut(s) 56, 57, 149
MunI CAATTG 1 cut(s) 160
NciI CCSGG 3 cut(s) 56, 57, 149
NdeII GATC 2 cut(s) 132, 508
NlaIII CATG 2 cut(s) 234, 457
NlaIV GGNNCC 1 cut(s) 144
NspI RCATGY 2 cut(s) 234, 457
PaeI GCATGC 1 cut(s) 234
PaeR7I CTCGAG 1 cut(s) 476
PaqCI CACCTGC 1 cut(s) 133
PciI ACATGT 1 cut(s) 453
PfeI GAWTC 2 cut(s) 209, 358
PkrI GCNGC 2 cut(s) 27, 111
PleI GAGTC 3 cut(s) 241, 363, 468
PpsI GAGTC 3 cut(s) 241, 363, 468
PscI ACATGT 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 144
PspXI VCTCGAGB 1 cut(s) 476
PstI CTGCAG 4 cut(s) 30, 130, 331, 399
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 2 cut(s) 26, 110
Sau3AI GATC 2 cut(s) 132, 508
SbfI CCTGCAGG 1 cut(s) 130
SchI GAGTC 3 cut(s) 241, 363, 468
ScrFI CCNGG 3 cut(s) 56, 57, 149
SdaI CCTGCAGG 1 cut(s) 130
SetI ASST 5 cut(s) 83, 89, 127, 196, 448
SfcI CTRYAG 4 cut(s) 26, 126, 327, 395
Sfr274I CTCGAG 1 cut(s) 476
SlaI CTCGAG 1 cut(s) 476
SmaI CCCGGG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
SphI GCATGC 1 cut(s) 234
Sse8387I CCTGCAGG 1 cut(s) 130
Sse9I AATT 3 cut(s) 160, 336, 436
SsiI CCGC 1 cut(s) 272
SspMI CTAG 1 cut(s) 529
StyD4I CCNGG 3 cut(s) 54, 55, 147
StyI CCWWGG 2 cut(s) 204, 215
TaqI TCGA 1 cut(s) 477
TasI AATT 3 cut(s) 160, 336, 436
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 2 cut(s) 209, 358
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 2 cut(s) 265, 469
TseI GCWGC 2 cut(s) 25, 109
TspDTI ATGAA 1 cut(s) 302
TspMI CCCGGG 1 cut(s) 55
TspRI CASTG 2 cut(s) 265, 469
XapI RAATTY 1 cut(s) 436
XceI RCATGY 2 cut(s) 234, 457
XhoI CTCGAG 1 cut(s) 476
XmaI CCCGGG 1 cut(s) 55
XspI CTAG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.