Rroxscaffold_3G00272590

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001877
Physical Location & Seq
Reverse (-)
64645899 .. 64649319
3421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272590.1

Sequence Viewer

Length: 558 bp
ATGGATGGCGCGAACTCCAAGCTCGCCGCACGAACTCCAAGCTCGCCGCACGAACTAGAACTCCGACCTAGCCGCACGAGAGGTGGTAGCGGGGTAGCAATGTCGACTCCTGCAAGGAAGAGGCTGATGAGAGACTTCAAGAGGCTGCAACAGGACCCACCTGCAGGGATTAGTGGGGCTCCCCAAGACAACAATATAATGCTGTGGAATGCCGTTATATTTGGTCCTGATGATACCCCTTGGGATGGAGGCACGTTTAAGTTGACTCTTCAGTTCACAGAAGATTATCCAAACAAACCACCAACGGTGCGATTTGTTTCTCGAATGTTTCATCCAAATATTTATGCCGATGGAAGTATTTGTTTAGATATTTTGCAAAATCAGTGGAGTCCTATTTATGATGTGGCAGCTATACTCACATCTATCCAGTCACTACTATGTGATCCGAACCCAAATTCTCCTGCAAATTCAGAAGCTGCTCGCATGTTCAGCGAGAACAAGCGAGAGTACAACCGAAGAGTTAGGGAAATCGTGGAACAGAGTTGGACAGCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

185

Amino Acids

20.74

Weight (kDa)

6.74

Isoelectric Point (pI)

69.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 41 - 177 1.1e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 11
AciI CCGC 4 cut(s) 27, 47, 73, 90
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 2 cut(s) 454, 466
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 2 cut(s) 164, 245
AgsI TTSAA 1 cut(s) 139
AloI GAACNNNNNNTCC 2 cut(s) 45, 77
AluBI AGCT 4 cut(s) 22, 42, 410, 476
AluI AGCT 4 cut(s) 22, 42, 410, 476
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 476
ApeKI GCWGC 3 cut(s) 145, 407, 476
ApoI RAATTY 2 cut(s) 454, 466
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 154, 224
AvaII GGWCC 2 cut(s) 154, 224
BanII GRGCYC 1 cut(s) 181
BauI CACGAG 1 cut(s) 76
BbvI GCAGC 3 cut(s) 132, 419, 463
BccI CCATC 2 cut(s) 239, 344
BceAI ACGGC 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 2 cut(s) 56, 69
BfmI CTRYAG 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 6 cut(s) 27, 47, 73, 146, 408, 477
BlsI GCNGC 6 cut(s) 28, 48, 74, 147, 409, 478
Bme18I GGWCC 2 cut(s) 154, 224
BmgT120I GGNCC 2 cut(s) 154, 224
BmiI GGNNCC 2 cut(s) 156, 180
BsaJI CCNNGG 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 245
Bse1I ACTGG 1 cut(s) 427
Bse3DI GCAATG 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 239
BseGI GGATG 3 cut(s) 10, 250, 331
BseLI CCNNNNNNNGG 2 cut(s) 164, 245
BseMI GCAATG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 427
BseXI GCAGC 3 cut(s) 132, 419, 463
Bsh1236I CGCG 1 cut(s) 11
BslI CCNNNNNNNGG 2 cut(s) 164, 245
BsmAI GTCTC 1 cut(s) 126
BsmI GAATGC 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 1 cut(s) 442
BspACI CCGC 4 cut(s) 27, 47, 73, 90
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 156, 180
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 105
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 239
BssMI GATC 1 cut(s) 442
BssSI CACGAG 1 cut(s) 76
BssT1I CCWWGG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 76
Bst4CI ACNGT 1 cut(s) 307
Bst6I CTCTTC 3 cut(s) 113, 273, 511
BstC8I GCNNGC 3 cut(s) 24, 44, 481
BstF5I GGATG 3 cut(s) 10, 250, 331
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 442
BstMWI GCNNNNNNNGC 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 132, 419, 463
BtsCI GGATG 3 cut(s) 10, 250, 331
BtsIMutI CAGTG 1 cut(s) 389
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 3 cut(s) 24, 44, 481
CaiI CAGNNNCTG 1 cut(s) 476
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 154, 224
Csp6I GTAC 1 cut(s) 508
CspCI CAANNNNNGTGG 2 cut(s) 365, 400
CviAII CATG 1 cut(s) 484
CviJI RGCY 8 cut(s) 22, 42, 72, 124, 145, 179, 410, 476
CviKI_1 RGCY 8 cut(s) 22, 42, 72, 124, 145, 179, 410, 476
CviQI GTAC 1 cut(s) 508
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
Eam1104I CTCTTC 3 cut(s) 113, 273, 511
EarI CTCTTC 3 cut(s) 113, 273, 511
Eco130I CCWWGG 1 cut(s) 239
Eco24I GRGCYC 1 cut(s) 181
Eco47I GGWCC 2 cut(s) 154, 224
Eco57I CTGAAG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 1 cut(s) 487
FaiI YATR 7 cut(s) 197, 218, 345, 399, 413, 439, 485
FatI CATG 1 cut(s) 483
FauI CCCGC 1 cut(s) 83
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 6 cut(s) 27, 47, 73, 146, 408, 477
FokI GGATG 3 cut(s) 17, 257, 318
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 6 cut(s) 27, 47, 73, 146, 408, 477
FspBI CTAG 2 cut(s) 56, 69
GlaI GCGC 1 cut(s) 10
GluI GCNGC 6 cut(s) 27, 47, 73, 146, 408, 477
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 487
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 2 cut(s) 105, 264
HindII GTYRAC 2 cut(s) 105, 264
HinfI GANTC 3 cut(s) 106, 265, 388
Hpy166II GTNNAC 3 cut(s) 105, 264, 276
Hpy188I TCNGA 3 cut(s) 65, 447, 472
Hpy188III TCNNGA 3 cut(s) 139, 227, 321
Hpy8I GTNNAC 3 cut(s) 105, 264, 276
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 5 cut(s) 113, 148, 164, 376, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 489
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 1 cut(s) 487
HspAI GCGC 1 cut(s) 9
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 7 cut(s) 123, 137, 150, 174, 240, 440, 474
Lsp1109I GCAGC 3 cut(s) 132, 419, 463
MaeI CTAG 2 cut(s) 56, 69
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 1 cut(s) 429
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 4 cut(s) 130, 260, 293, 528
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 2 cut(s) 454, 466
MlyI GAGTC 3 cut(s) 100, 259, 397
MmeI TCCRAC 2 cut(s) 88, 524
MnlI CCTC 4 cut(s) 74, 114, 135, 242
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 436
Mva1269I GAATGC 1 cut(s) 214
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 489
NdeII GATC 1 cut(s) 442
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 2 cut(s) 156, 180
NmuCI GTSAC 1 cut(s) 429
NspI RCATGY 1 cut(s) 487
PaqCI CACCTGC 1 cut(s) 169
PctI GAATGC 1 cut(s) 214
PkrI GCNGC 6 cut(s) 28, 48, 74, 147, 409, 478
PleI GAGTC 3 cut(s) 100, 259, 396
PpsI GAGTC 3 cut(s) 100, 259, 396
PpuMI RGGWCCY 1 cut(s) 154
Psp5II RGGWCCY 1 cut(s) 154
PspN4I GGNNCC 2 cut(s) 156, 180
PspPI GGNCC 2 cut(s) 154, 224
PspPPI RGGWCCY 1 cut(s) 154
PstI CTGCAG 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 476
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 436
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 6 cut(s) 27, 47, 73, 146, 408, 477
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 2 cut(s) 154, 224
SbfI CCTGCAGG 1 cut(s) 166
SchI GAGTC 3 cut(s) 100, 259, 397
SdaI CCTGCAGG 1 cut(s) 166
SduI GDGCHC 1 cut(s) 181
SetI ASST 8 cut(s) 24, 44, 70, 85, 163, 257, 412, 478
SfcI CTRYAG 1 cut(s) 162
SinI GGWCC 2 cut(s) 154, 224
SmiMI CAYNNNNRTG 1 cut(s) 436
Sse8387I CCTGCAGG 1 cut(s) 166
Sse9I AATT 2 cut(s) 454, 466
SsiI CCGC 4 cut(s) 27, 47, 73, 90
SspI AATATT 1 cut(s) 340
SspMI CTAG 2 cut(s) 56, 69
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 1 cut(s) 307
TaiI ACGT 1 cut(s) 257
TaqI TCGA 2 cut(s) 104, 322
TasI AATT 2 cut(s) 454, 466
TatI WGTACW 1 cut(s) 507
TauI GCSGC 3 cut(s) 29, 49, 75
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 3 cut(s) 145, 407, 476
Tsp45I GTSAC 1 cut(s) 429
TspDTI ATGAA 1 cut(s) 320
TspRI CASTG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 154, 224
XapI RAATTY 2 cut(s) 454, 466
XceI RCATGY 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 104
XspI CTAG 2 cut(s) 56, 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.