RchiOBHm_Chr3g0450881

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001877
Physical Location & Seq
Forward (+)
2244866 .. 2246745
1880 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41819

Sequence Viewer

Length: 744 bp
ATGATGTTGACTCCTTCAATCAAGACTCTGTCGAGGGAGTTGGATATGCTGCGGATTGAAGAAGACCCGCCTCCGGGTGTGGAGATGAGTATGGTGCTACCTGACTTGGACAGATTGATGCTTGTACAAGACCCACCTGCTGGCGCGGAGATTAGTTTGGTGCTCCCTGGTTTGGACAGATTGCGGCTTGTAGAACACTCAACCGCAGGCGGAGAGGATGTTTTTGCAAGGCTGCAGAAAAAAGTGTCGGATTACCCACCCCCGGGCGCAGATTATACTCGCGCAAGGTATAGGCTGTTTAAAGAGTTGAAGAGGCTGCTACAAGACCCACCTGCAGGGATCAGTGTGGTTCCCCCGGACTTCTACAATTGCGATATAATGTTGTGGAATGTTGTTATAGCTCCTCACTCCTTGGATTCTCCTTGGGAGGGAGGCGCATTTAAGTTGACTCTTCAGTTTAGTGAGGATTATCCACTCAAACCACCAATGGTGCGATTTGTTTCTCAAATGTTTCATCCAAACATTTCTGCAGATGGAATTATGTCTTTTAATAATTTACTGGATCGGTGGCCTTCTATAAATGACGTGGAAGATATACTCAAGTATATCCAGTCCCTAATATGTCATCCCAACCCGAAGTGTCCTGCAAATTCTGAAGCTTTCTACATGTACTTCAGTGAGAACAGACTGGAGTACAACCGAAGAGTGAGGATGATCGTGGATCAGAGTTGGATGGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

247

Amino Acids

28.29

Weight (kDa)

5.0

Isoelectric Point (pI)

60.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 98 - 238 4e-33 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 145, 340
Acc36I ACCTGC 2 cut(s) 145, 340
AccB7I CCANNNNNTGG 1 cut(s) 140
AccII CGCG 2 cut(s) 146, 282
AciI CCGC 6 cut(s) 52, 68, 146, 184, 204, 210
AclWI GGATC 3 cut(s) 347, 570, 729
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 3 cut(s) 437, 658, 675
AfaI GTAC 3 cut(s) 126, 671, 695
AfiI CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 3 cut(s) 18, 59, 310
AjiI CACGTC 1 cut(s) 586
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 2 cut(s) 401, 659
AluI AGCT 2 cut(s) 401, 659
Alw21I GWGCWC 1 cut(s) 165
AlwI GGATC 3 cut(s) 347, 570, 729
Ama87I CYCGRG 1 cut(s) 262
AoxI GGCC 1 cut(s) 569
ApeKI GCWGC 3 cut(s) 49, 232, 316
ApoI RAATTY 1 cut(s) 649
AspLEI GCGC 4 cut(s) 146, 269, 284, 437
AsuC2I CCSGG 4 cut(s) 75, 263, 264, 356
AvaI CYCGRG 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 3 cut(s) 36, 219, 303
BccI CCATC 2 cut(s) 527, 727
BciT130I CCWGG 1 cut(s) 168
BcnI CCSGG 4 cut(s) 75, 263, 264, 356
BfaI CTAG 1 cut(s) 742
BfmI CTRYAG 3 cut(s) 233, 333, 528
BfuAI ACCTGC 2 cut(s) 145, 340
BisI GCNGC 4 cut(s) 50, 185, 233, 317
BlsI GCNGC 4 cut(s) 51, 186, 234, 318
Bme1390I CCNGG 5 cut(s) 75, 168, 263, 264, 356
BmeT110I CYCGRG 1 cut(s) 262
BmgBI CACGTC 1 cut(s) 586
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 5 cut(s) 75, 168, 263, 264, 356
BmsI GCATC 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 4 cut(s) 75, 263, 264, 356
BsaJI CCNNGG 6 cut(s) 166, 261, 262, 354, 411, 422
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
Bse1I ACTGG 3 cut(s) 564, 610, 693
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 6 cut(s) 166, 261, 262, 354, 411, 422
BseGI GGATG 5 cut(s) 223, 514, 625, 717, 738
BseLI CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
BseNI ACTGG 3 cut(s) 564, 610, 693
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 3 cut(s) 36, 219, 303
Bsh1236I CGCG 2 cut(s) 146, 282
BshFI GGCC 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 262
BsiSI CCGG 3 cut(s) 74, 263, 356
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 1 cut(s) 571
BsoBI CYCGRG 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 165
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 4 cut(s) 339, 562, 714, 721
BspACI CCGC 6 cut(s) 52, 68, 146, 184, 204, 210
BspANI GGCC 1 cut(s) 571
BspFNI CGCG 2 cut(s) 146, 282
BspLI GGNNCC 1 cut(s) 351
BspMAI CTGCAG 3 cut(s) 237, 337, 532
BspMI ACCTGC 2 cut(s) 145, 340
BspPI GGATC 3 cut(s) 347, 570, 729
BsrGI TGTACA 1 cut(s) 124
BsrI ACTGG 3 cut(s) 564, 610, 693
BssECI CCNNGG 6 cut(s) 166, 261, 262, 354, 411, 422
BssMI GATC 4 cut(s) 339, 562, 714, 721
BssT1I CCWWGG 2 cut(s) 411, 422
Bst2UI CCWGG 1 cut(s) 168
Bst6I CTCTTC 3 cut(s) 305, 456, 697
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 142, 208
BstF5I GGATG 5 cut(s) 223, 514, 625, 717, 738
BstFNI CGCG 2 cut(s) 146, 282
BstHHI GCGC 4 cut(s) 146, 269, 284, 437
BstKTI GATC 4 cut(s) 342, 565, 717, 724
BstMBI GATC 4 cut(s) 339, 562, 714, 721
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 5 cut(s) 73, 166, 261, 262, 354
BstSFI CTRYAG 3 cut(s) 233, 333, 528
BstUI CGCG 2 cut(s) 146, 282
BstV1I GCAGC 3 cut(s) 36, 219, 303
BstV2I GAAGAC 1 cut(s) 69
BsuRI GGCC 1 cut(s) 571
BtrI CACGTC 1 cut(s) 586
BtsCI GGATG 5 cut(s) 223, 514, 625, 717, 738
BtsIMutI CAGTG 2 cut(s) 349, 682
BveI ACCTGC 2 cut(s) 145, 340
Cac8I GCNNGC 2 cut(s) 142, 208
CfoI GCGC 4 cut(s) 146, 269, 284, 437
Cfr9I CCCGGG 1 cut(s) 262
Csp6I GTAC 3 cut(s) 125, 670, 694
CviAII CATG 1 cut(s) 667
CviJI RGCY 7 cut(s) 187, 232, 295, 316, 401, 571, 659
CviKI_1 RGCY 7 cut(s) 187, 232, 295, 316, 401, 571, 659
CviQI GTAC 3 cut(s) 125, 670, 694
DpnI GATC 4 cut(s) 341, 564, 716, 723
DpnII GATC 4 cut(s) 339, 562, 714, 721
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 3 cut(s) 305, 456, 697
EarI CTCTTC 3 cut(s) 305, 456, 697
EciI GGCGGA 1 cut(s) 225
Eco130I CCWWGG 2 cut(s) 411, 422
Eco57I CTGAAG 3 cut(s) 437, 658, 675
Eco88I CYCGRG 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 2 cut(s) 411, 422
ErhI CCWWGG 2 cut(s) 411, 422
FaeI CATG 1 cut(s) 670
FaqI GGGAC 1 cut(s) 598
FatI CATG 1 cut(s) 666
FauI CCCGC 1 cut(s) 75
Fnu4HI GCNGC 4 cut(s) 50, 185, 233, 317
FokI GGATG 4 cut(s) 230, 501, 612, 724
Fsp4HI GCNGC 4 cut(s) 50, 185, 233, 317
FspBI CTAG 1 cut(s) 742
GlaI GCGC 4 cut(s) 145, 268, 283, 436
GluI GCNGC 4 cut(s) 50, 185, 233, 317
GsuI CTGGAG 1 cut(s) 710
HaeIII GGCC 1 cut(s) 571
HapII CCGG 3 cut(s) 74, 263, 356
HhaI GCGC 4 cut(s) 146, 269, 284, 437
Hin1II CATG 1 cut(s) 670
Hin6I GCGC 4 cut(s) 144, 267, 282, 435
HinP1I GCGC 4 cut(s) 144, 267, 282, 435
HincII GTYRAC 2 cut(s) 9, 447
HindII GTYRAC 2 cut(s) 9, 447
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 4 cut(s) 10, 25, 416, 448
HpaII CCGG 3 cut(s) 74, 263, 356
Hpy166II GTNNAC 2 cut(s) 9, 447
Hpy188I TCNGA 3 cut(s) 250, 655, 726
Hpy188III TCNNGA 1 cut(s) 22
Hpy8I GTNNAC 2 cut(s) 9, 447
HpyAV CCTTC 2 cut(s) 24, 582
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 5 cut(s) 227, 235, 335, 530, 647
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 1 cut(s) 670
HspAI GCGC 4 cut(s) 144, 267, 282, 435
Kzo9I GATC 4 cut(s) 339, 562, 714, 721
LmnI GCTCC 2 cut(s) 168, 406
Lsp1109I GCAGC 3 cut(s) 36, 219, 303
LweI GCATC 1 cut(s) 108
MaeI CTAG 1 cut(s) 742
MaeII ACGT 1 cut(s) 585
MalI GATC 4 cut(s) 341, 564, 716, 723
MboI GATC 4 cut(s) 339, 562, 714, 721
MboII GAAGA 6 cut(s) 71, 74, 322, 443, 602, 714
MfeI CAATTG 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 4 cut(s) 367, 537, 553, 649
MlyI GAGTC 3 cut(s) 4, 19, 442
MmeI TCCRAC 3 cut(s) 21, 228, 710
MnlI CCTC 9 cut(s) 27, 81, 208, 306, 414, 421, 425, 457, 702
MseI TTAA 3 cut(s) 300, 441, 549
MspI CCGG 3 cut(s) 74, 263, 356
MspR9I CCNGG 5 cut(s) 75, 168, 263, 264, 356
MunI CAATTG 1 cut(s) 367
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 2 cut(s) 146, 282
NciI CCSGG 4 cut(s) 75, 263, 264, 356
NdeII GATC 4 cut(s) 339, 562, 714, 721
NlaIII CATG 1 cut(s) 670
NlaIV GGNNCC 1 cut(s) 351
NspI RCATGY 1 cut(s) 670
PaqCI CACCTGC 2 cut(s) 145, 340
PciI ACATGT 1 cut(s) 666
PfeI GAWTC 1 cut(s) 416
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 140
PkrI GCNGC 4 cut(s) 51, 186, 234, 318
PleI GAGTC 3 cut(s) 4, 19, 442
PpsI GAGTC 3 cut(s) 4, 19, 442
PscI ACATGT 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 351
PstI CTGCAG 3 cut(s) 237, 337, 532
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 3 cut(s) 126, 671, 695
RsaNI GTAC 3 cut(s) 125, 670, 694
SaqAI TTAA 3 cut(s) 300, 441, 549
SatI GCNGC 4 cut(s) 50, 185, 233, 317
Sau3AI GATC 4 cut(s) 339, 562, 714, 721
SbfI CCTGCAGG 1 cut(s) 337
SchI GAGTC 3 cut(s) 4, 19, 442
ScrFI CCNGG 5 cut(s) 75, 168, 263, 264, 356
SdaI CCTGCAGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 165
SetI ASST 7 cut(s) 103, 139, 290, 334, 403, 588, 661
SfaNI GCATC 1 cut(s) 108
SfcI CTRYAG 3 cut(s) 233, 333, 528
SmaI CCCGGG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse8387I CCTGCAGG 1 cut(s) 337
Sse9I AATT 4 cut(s) 367, 537, 553, 649
SsiI CCGC 6 cut(s) 52, 68, 146, 184, 204, 210
SspMI CTAG 1 cut(s) 742
StyD4I CCNGG 5 cut(s) 73, 166, 261, 262, 354
StyI CCWWGG 2 cut(s) 411, 422
TaiI ACGT 1 cut(s) 588
TaqI TCGA 1 cut(s) 32
TasI AATT 4 cut(s) 367, 537, 553, 649
TatI WGTACW 3 cut(s) 124, 669, 693
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 3 cut(s) 300, 441, 549
Tru9I TTAA 3 cut(s) 300, 441, 549
TscAI CASTG 2 cut(s) 349, 682
TseI GCWGC 3 cut(s) 49, 232, 316
TspDTI ATGAA 1 cut(s) 503
TspMI CCCGGG 1 cut(s) 262
TspRI CASTG 2 cut(s) 349, 682
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 649
XceI RCATGY 1 cut(s) 670
XmaI CCCGGG 1 cut(s) 262
XspI CTAG 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.