MD06G1189200.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001877
Physical Location & Seq
Forward (+)
32616538 .. 32618838
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1189200.v1.1.491

Sequence Viewer

Length: 432 bp
ATGAGAGACTTCAAGAGGCTGCAACAGGACCCGCCTGCAGGGATTAGTGGGGCGCCACAGGATAACAATATAATGTTGTGGAATGCTGTTATATTTGGTCCCGATGATACACCTTGGGATGGAGGCACATTTAAGTTGACTCTTCAGTTTACGGAGGATTATCCAAACAAACCACCAACAGTCCGGTTTGTTTCTAGAATGTTTCATCCAAATATTTATGCGGATGGAAGTATTTGTTTGGATATTTTACAGAATCAGTGGAGTCCTATATATGACGTGGCAGCTATACTCACGTCCATCCAGTCACTACTATGTGATCCCAACCCAAACTCTCCTGCAAATTCAGAAGCTGCTCGCATGTTTAGCGAGAACAAGCGTGAGTACAACCGAAGAGTGAGGGAAATCGTGGAACAGAGTTGGACAGCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

144

Amino Acids

16.31

Weight (kDa)

4.79

Isoelectric Point (pI)

70.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 1 - 135 5.1e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 52
AciI CCGC 2 cut(s) 32, 221
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 128
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 2 cut(s) 38, 119
AgsI TTSAA 1 cut(s) 13
AjiI CACGTC 2 cut(s) 277, 294
AluBI AGCT 2 cut(s) 284, 350
AluI AGCT 2 cut(s) 284, 350
AlwI GGATC 1 cut(s) 311
AlwNI CAGNNNCTG 1 cut(s) 350
ApeKI GCWGC 3 cut(s) 19, 281, 350
ApoI RAATTY 1 cut(s) 340
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 28, 98
AvaII GGWCC 2 cut(s) 28, 98
BanI GGYRCC 1 cut(s) 52
BbvI GCAGC 3 cut(s) 6, 293, 337
BccI CCATC 3 cut(s) 113, 218, 305
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 1 cut(s) 56
BisI GCNGC 3 cut(s) 20, 282, 351
BlsI GCNGC 3 cut(s) 21, 283, 352
Bme18I GGWCC 2 cut(s) 28, 98
BmgBI CACGTC 2 cut(s) 277, 294
BmgT120I GGNCC 2 cut(s) 28, 98
BmiI GGNNCC 3 cut(s) 30, 54, 100
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 113
BsaWI WCCGGW 1 cut(s) 183
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 119
Bse1I ACTGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 4 cut(s) 124, 205, 229, 297
BseLI CCNNNNNNNGG 2 cut(s) 38, 119
BseNI ACTGG 1 cut(s) 301
BseXI GCAGC 3 cut(s) 6, 293, 337
BshNI GGYRCC 1 cut(s) 52
BsiSI CCGG 1 cut(s) 184
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 2 cut(s) 38, 119
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 88
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 2 cut(s) 32, 221
BspLI GGNNCC 3 cut(s) 30, 54, 100
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 52
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 1 cut(s) 316
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 147, 385
BstACI GRCGYC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 36, 355
BstF5I GGATG 4 cut(s) 124, 205, 229, 297
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 1 cut(s) 319
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 3 cut(s) 6, 293, 337
BtrI CACGTC 2 cut(s) 277, 294
BtsCI GGATG 4 cut(s) 124, 205, 229, 297
BtsIMutI CAGTG 1 cut(s) 263
Cac8I GCNNGC 2 cut(s) 36, 355
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 28, 98
Csp6I GTAC 1 cut(s) 382
CviAII CATG 1 cut(s) 358
CviJI RGCY 3 cut(s) 19, 284, 350
CviKI_1 RGCY 3 cut(s) 19, 284, 350
CviQI GTAC 1 cut(s) 382
DinI GGCGCC 1 cut(s) 54
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 147, 385
EarI CTCTTC 2 cut(s) 147, 385
Eco130I CCWWGG 1 cut(s) 113
Eco47I GGWCC 2 cut(s) 28, 98
Eco57I CTGAAG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 113
EgeI GGCGCC 1 cut(s) 54
EheI GGCGCC 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 1 cut(s) 361
FaiI YATR 9 cut(s) 71, 92, 219, 269, 271, 273, 287, 313, 359
FaqI GGGAC 1 cut(s) 84
FatI CATG 1 cut(s) 357
FauI CCCGC 1 cut(s) 39
Fnu4HI GCNGC 3 cut(s) 20, 282, 351
FokI GGATG 4 cut(s) 131, 192, 236, 284
Fsp4HI GCNGC 3 cut(s) 20, 282, 351
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 54
GluI GCNGC 3 cut(s) 20, 282, 351
HaeII RGCGCY 1 cut(s) 56
HapII CCGG 1 cut(s) 184
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 3 cut(s) 139, 253, 262
HpaII CCGG 1 cut(s) 184
Hpy166II GTNNAC 2 cut(s) 138, 150
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 3 cut(s) 13, 101, 195
Hpy8I GTNNAC 2 cut(s) 138, 150
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 2 cut(s) 276, 293
HpyCH4V TGCA 3 cut(s) 22, 38, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 363
HpySE526I ACGT 2 cut(s) 276, 293
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 1 cut(s) 53
KasI GGCGCC 1 cut(s) 52
Kzo9I GATC 1 cut(s) 316
LpnPI CCDG 7 cut(s) 11, 24, 44, 48, 197, 314, 348
Lsp1109I GCAGC 3 cut(s) 6, 293, 337
MaeI CTAG 1 cut(s) 195
MaeII ACGT 2 cut(s) 276, 293
MaeIII GTNAC 1 cut(s) 303
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 2 cut(s) 134, 402
MluCI AATT 1 cut(s) 340
Mly113I GGCGCC 1 cut(s) 53
MlyI GAGTC 2 cut(s) 133, 271
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 4 cut(s) 9, 116, 148, 390
MseI TTAA 1 cut(s) 132
MslI CAYNNNNRTG 1 cut(s) 310
MspI CCGG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 363
NarI GGCGCC 1 cut(s) 53
NdeII GATC 1 cut(s) 316
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 3 cut(s) 30, 54, 100
NmuCI GTSAC 1 cut(s) 303
NspI RCATGY 1 cut(s) 361
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 3 cut(s) 21, 283, 352
PleI GAGTC 2 cut(s) 133, 270
PluTI GGCGCC 1 cut(s) 56
PpsI GAGTC 2 cut(s) 133, 270
PpuMI RGGWCCY 1 cut(s) 28
Psp5II RGGWCCY 1 cut(s) 28
PspN4I GGNNCC 3 cut(s) 30, 54, 100
PspPI GGNCC 2 cut(s) 28, 98
PspPPI RGGWCCY 1 cut(s) 28
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 350
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 310
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 3 cut(s) 20, 282, 351
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 2 cut(s) 28, 98
SbfI CCTGCAGG 1 cut(s) 40
SchI GAGTC 2 cut(s) 133, 271
SdaI CCTGCAGG 1 cut(s) 40
SetI ASST 5 cut(s) 115, 279, 286, 296, 352
SfcI CTRYAG 1 cut(s) 36
SfoI GGCGCC 1 cut(s) 54
SinI GGWCC 2 cut(s) 28, 98
SmiMI CAYNNNNRTG 1 cut(s) 310
Sse8387I CCTGCAGG 1 cut(s) 40
Sse9I AATT 1 cut(s) 340
SsiI CCGC 2 cut(s) 32, 221
SspDI GGCGCC 1 cut(s) 52
SspI AATATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 2 cut(s) 279, 296
TasI AATT 1 cut(s) 340
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 1 cut(s) 263
TseFI GTSAC 1 cut(s) 303
TseI GCWGC 3 cut(s) 19, 281, 350
Tsp45I GTSAC 1 cut(s) 303
TspDTI ATGAA 1 cut(s) 194
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 28, 98
XapI RAATTY 1 cut(s) 340
XbaI TCTAGA 1 cut(s) 194
XceI RCATGY 1 cut(s) 361
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.