Rh3BG032200

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001877
Physical Location & Seq
Forward (+)
2127148 .. 2128096
949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG032200.1

Sequence Viewer

Length: 495 bp
ATGGTGCTACCTGACTTGGACAGATTGCGGCTTGTACAAGACCCACCTGCTGGCGCGGAGATTAGTATGGTGCTCCCAGGTTTGGACAGATTGCGGCTTGCAGAACACTCAAGCGCAGGTGGAGAGGATGTTTTTGCAAGCTCCTCATTCAATTCCTTGGATTCTCCTTGGGAGGGAGGCGCATGCACATTTAAGTTGACTCTTCAGTTCAGTGAGGATTATCCGCTCAAACCATCAATGGTGCGATTTGTTTCTCAAATGTTTCATCCAAACATTTCTGCAGATGGAAGTATCTCTTTTAATAATTTACTGGATCGGTGGGCTCCTATAAATGATGTGGAAGATATACTCAAGTATATCCAGTCCCTAATATGTCATCCCAACCCGAAGTGTCCTGCAAATTCAGAAGCTTTCTACATGTATTTCAGCGAGAACAGACTCGAGTACAACCGAAGAGTGAGGATGATTGTGGATCAGAGTTGTATGGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

164

Amino Acids

18.51

Weight (kDa)

4.79

Isoelectric Point (pI)

67.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 54 - 155 6.7e-27 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 55, 107
Acc36I ACCTGC 2 cut(s) 55, 107
AccB7I CCANNNNNTGG 1 cut(s) 50
AccBSI CCGCTC 1 cut(s) 226
AccII CGCG 1 cut(s) 56
AciI CCGC 4 cut(s) 28, 56, 94, 224
AclWI GGATC 2 cut(s) 321, 480
AcsI RAATTY 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 2 cut(s) 36, 446
AfiI CCNNNNNNNGG 3 cut(s) 50, 82, 173
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 2 cut(s) 141, 410
AluI AGCT 2 cut(s) 141, 410
Alw21I GWGCWC 1 cut(s) 75
AlwI GGATC 2 cut(s) 321, 480
Ama87I CYCGRG 1 cut(s) 440
ApoI RAATTY 1 cut(s) 400
AspLEI GCGC 3 cut(s) 56, 116, 182
AvaI CYCGRG 1 cut(s) 440
BanII GRGCYC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 75
BccI CCATC 2 cut(s) 241, 278
BciT130I CCWGG 1 cut(s) 78
BfaI CTAG 1 cut(s) 493
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 2 cut(s) 55, 107
BisI GCNGC 2 cut(s) 29, 95
BlsI GCNGC 2 cut(s) 30, 96
Bme1390I CCNGG 1 cut(s) 78
BmeT110I CYCGRG 1 cut(s) 440
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 94, 335
BsaJI CCNNGG 3 cut(s) 76, 156, 167
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 82, 173
Bse1I ACTGG 2 cut(s) 315, 361
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 76, 156, 167
BseGI GGATG 4 cut(s) 133, 265, 376, 468
BseLI CCNNNNNNNGG 3 cut(s) 50, 82, 173
BseNI ACTGG 2 cut(s) 315, 361
BseRI GAGGAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 3 cut(s) 50, 82, 173
BsmFI GGGAC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 75, 325
Bsp1407I TGTACA 1 cut(s) 34
Bsp143I GATC 2 cut(s) 313, 472
BspACI CCGC 4 cut(s) 28, 56, 94, 224
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 2 cut(s) 55, 107
BspPI GGATC 2 cut(s) 321, 480
BsrBI CCGCTC 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 34
BsrI ACTGG 2 cut(s) 315, 361
BssECI CCNNGG 3 cut(s) 76, 156, 167
BssMI GATC 2 cut(s) 313, 472
BssT1I CCWWGG 2 cut(s) 156, 167
Bst2UI CCWGG 1 cut(s) 78
Bst6I CTCTTC 2 cut(s) 207, 448
BstAUI TGTACA 1 cut(s) 34
BstC8I GCNNGC 4 cut(s) 52, 99, 139, 184
BstF5I GGATG 4 cut(s) 133, 265, 376, 468
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 3 cut(s) 56, 116, 182
BstKTI GATC 2 cut(s) 316, 475
BstMBI GATC 2 cut(s) 313, 472
BstNI CCWGG 1 cut(s) 78
BstNSI RCATGY 2 cut(s) 186, 421
BstSCI CCNGG 1 cut(s) 76
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 1 cut(s) 56
BtsCI GGATG 4 cut(s) 133, 265, 376, 468
BtsIMutI CAGTG 1 cut(s) 217
BveI ACCTGC 2 cut(s) 55, 107
Cac8I GCNNGC 4 cut(s) 52, 99, 139, 184
CfoI GCGC 3 cut(s) 56, 116, 182
Csp6I GTAC 2 cut(s) 35, 445
CviAII CATG 2 cut(s) 183, 418
CviJI RGCY 5 cut(s) 31, 97, 141, 323, 410
CviKI_1 RGCY 5 cut(s) 31, 97, 141, 323, 410
CviQI GTAC 2 cut(s) 35, 445
DpnI GATC 2 cut(s) 315, 474
DpnII GATC 2 cut(s) 313, 472
Eam1104I CTCTTC 2 cut(s) 207, 448
EarI CTCTTC 2 cut(s) 207, 448
Eco130I CCWWGG 2 cut(s) 156, 167
Eco24I GRGCYC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 76
EcoT14I CCWWGG 2 cut(s) 156, 167
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 156, 167
FaeI CATG 2 cut(s) 186, 421
FaiI YATR 8 cut(s) 68, 184, 329, 347, 357, 373, 419, 485
FalI AAGNNNNNCTT 2 cut(s) 280, 312
FaqI GGGAC 1 cut(s) 349
FatI CATG 2 cut(s) 182, 417
Fnu4HI GCNGC 2 cut(s) 29, 95
FokI GGATG 4 cut(s) 140, 252, 363, 475
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 2 cut(s) 29, 95
FspBI CTAG 1 cut(s) 493
GlaI GCGC 3 cut(s) 55, 115, 181
GluI GCNGC 2 cut(s) 29, 95
HhaI GCGC 3 cut(s) 56, 116, 182
Hin1II CATG 2 cut(s) 186, 421
Hin6I GCGC 3 cut(s) 54, 114, 180
HinP1I GCGC 3 cut(s) 54, 114, 180
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 3 cut(s) 161, 199, 438
Hpy166II GTNNAC 1 cut(s) 198
Hpy188I TCNGA 2 cut(s) 406, 477
Hpy8I GTNNAC 1 cut(s) 198
HpyCH4V TGCA 5 cut(s) 101, 137, 186, 281, 398
Hsp92II CATG 2 cut(s) 186, 421
HspAI GCGC 3 cut(s) 54, 114, 180
Kzo9I GATC 2 cut(s) 313, 472
LmnI GCTCC 3 cut(s) 78, 146, 328
LpnPI CCDG 9 cut(s) 24, 36, 60, 63, 90, 102, 296, 374, 408
MaeI CTAG 1 cut(s) 493
MalI GATC 2 cut(s) 315, 474
MbiI CCGCTC 1 cut(s) 226
MboI GATC 2 cut(s) 313, 472
MboII GAAGA 3 cut(s) 194, 353, 465
MhlI GDGCHC 2 cut(s) 75, 325
MluCI AATT 3 cut(s) 151, 304, 400
MlyI GAGTC 2 cut(s) 193, 432
MnlI CCTC 6 cut(s) 118, 154, 166, 170, 208, 453
MseI TTAA 2 cut(s) 192, 300
MspR9I CCNGG 1 cut(s) 78
MvaI CCWGG 1 cut(s) 78
MvnI CGCG 1 cut(s) 56
NdeII GATC 2 cut(s) 313, 472
NlaIII CATG 2 cut(s) 186, 421
NlaIV GGNNCC 1 cut(s) 324
NspI RCATGY 2 cut(s) 186, 421
PaeI GCATGC 1 cut(s) 186
PaeR7I CTCGAG 1 cut(s) 440
PaqCI CACCTGC 2 cut(s) 55, 107
PciI ACATGT 1 cut(s) 417
PfeI GAWTC 1 cut(s) 161
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 2 cut(s) 30, 96
PleI GAGTC 2 cut(s) 193, 432
PpsI GAGTC 2 cut(s) 193, 432
PscI ACATGT 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 324
PspXI VCTCGAGB 1 cut(s) 440
PstI CTGCAG 1 cut(s) 283
RsaI GTAC 2 cut(s) 36, 446
RsaNI GTAC 2 cut(s) 35, 445
SaqAI TTAA 2 cut(s) 192, 300
SatI GCNGC 2 cut(s) 29, 95
Sau3AI GATC 2 cut(s) 313, 472
SchI GAGTC 2 cut(s) 193, 432
ScrFI CCNGG 1 cut(s) 78
SduI GDGCHC 2 cut(s) 75, 325
SetI ASST 6 cut(s) 13, 49, 82, 121, 143, 412
SfcI CTRYAG 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 440
SlaI CTCGAG 1 cut(s) 440
SmlI CTYRAG 3 cut(s) 109, 350, 440
SmoI CTYRAG 3 cut(s) 109, 350, 440
SphI GCATGC 1 cut(s) 186
Sse9I AATT 3 cut(s) 151, 304, 400
SsiI CCGC 4 cut(s) 28, 56, 94, 224
SspMI CTAG 1 cut(s) 493
StyD4I CCNGG 1 cut(s) 76
StyI CCWWGG 2 cut(s) 156, 167
TaqI TCGA 1 cut(s) 441
TasI AATT 3 cut(s) 151, 304, 400
TatI WGTACW 2 cut(s) 34, 444
TauI GCSGC 2 cut(s) 31, 97
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 192, 300
Tru9I TTAA 2 cut(s) 192, 300
TscAI CASTG 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 254
TspRI CASTG 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 50
XapI RAATTY 1 cut(s) 400
XceI RCATGY 2 cut(s) 186, 421
XhoI CTCGAG 1 cut(s) 440
XspI CTAG 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.