Rroxscaffold_6G00428500

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001877
Physical Location & Seq
Forward (+)
48914900 .. 48916852
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428500.1

Sequence Viewer

Length: 744 bp
ATGATGTTGACTCCTTCAATCAAGACTCTGTCGAGGGAGTTGGATATGCTGCGGATTGAAGAAGACCCGCCTCCGGGTGTGGAGATGAGTATGGTGCTACCTGACTTGGACAGATTGCGGCTTGTACAAGACCCACCTGCTGGCACGGAGATTAGTATGGTGCTCCCAGTTTTGGACAGGTTGCGGCTTGTAGAACACTCAACTGCATGCGGAGAGGATGTTTTTGCAAGGCTGCAGAAAAAGGTGTCGGATTACCCACCCCCGGGCGCAGATTACACTCGTGCAAGGTATAGGCTGTTTAAAGAGTTGAAGAGGCTGCTACAAGACCCACCTGCAGGGATCAGTGTGGTTCCCCCAGACTTCTACAATTGCGATATAATGTTGTGGAATGTTGTTATAGCTCCTCATTCCTTGGATTCTCCTTGGGAGGGAGGTGCATTTAAGTTGACTCTTCAGTTTAGTGAGGATTATCCACTCAAACCACCAATGGTGCGATTTGTTTCTCAAATGTTTCATCCAAACATTTCTGCAGATGGAATTATGTCTTTTAATAATTTACTGGATCGGTGGCCTTCTATAAATGATGTGGAAGATATACTCAAGTATATCCAGTCCCTAATATGTCATCCCAACCCGAAGTGGCCTGCAAATTCTGAAGCTTTCTACATGTACTTCAGCGAGAACAGACTGGAGTACAACCGAAGAGTGAGGCTGATCGTGGATCAGAGTTGGATGGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

247

Amino Acids

28.51

Weight (kDa)

5.09

Isoelectric Point (pI)

63.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 98 - 238 5.8e-33 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 145, 340
Acc36I ACCTGC 2 cut(s) 145, 340
AccB7I CCANNNNNTGG 1 cut(s) 140
AciI CCGC 5 cut(s) 52, 68, 118, 184, 210
AclWI GGATC 3 cut(s) 347, 570, 729
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 3 cut(s) 437, 658, 675
AfaI GTAC 3 cut(s) 126, 671, 695
AfiI CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 3 cut(s) 18, 59, 310
AluBI AGCT 2 cut(s) 401, 659
AluI AGCT 2 cut(s) 401, 659
Alw21I GWGCWC 1 cut(s) 165
AlwI GGATC 3 cut(s) 347, 570, 729
Ama87I CYCGRG 1 cut(s) 262
AoxI GGCC 2 cut(s) 569, 641
ApeKI GCWGC 3 cut(s) 49, 232, 316
ApoI RAATTY 1 cut(s) 649
AspLEI GCGC 1 cut(s) 269
AsuC2I CCSGG 3 cut(s) 75, 263, 264
AvaI CYCGRG 1 cut(s) 262
BauI CACGAG 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 165
BbvI GCAGC 3 cut(s) 36, 219, 303
BccI CCATC 2 cut(s) 527, 727
BcnI CCSGG 3 cut(s) 75, 263, 264
BfaI CTAG 1 cut(s) 742
BfmI CTRYAG 3 cut(s) 233, 333, 528
BfuAI ACCTGC 2 cut(s) 145, 340
BisI GCNGC 5 cut(s) 50, 119, 185, 233, 317
BlsI GCNGC 5 cut(s) 51, 120, 186, 234, 318
Bme1390I CCNGG 3 cut(s) 75, 263, 264
BmeT110I CYCGRG 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 3 cut(s) 75, 263, 264
BmrI ACTGGG 1 cut(s) 161
BmuI ACTGGG 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 3 cut(s) 75, 263, 264
BsaJI CCNNGG 4 cut(s) 261, 262, 411, 422
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
Bse1I ACTGG 4 cut(s) 167, 564, 610, 693
BseDI CCNNGG 4 cut(s) 261, 262, 411, 422
BseGI GGATG 4 cut(s) 223, 514, 625, 738
BseLI CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
BseNI ACTGG 4 cut(s) 167, 564, 610, 693
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 3 cut(s) 36, 219, 303
BshFI GGCC 2 cut(s) 571, 643
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 262
BsiSI CCGG 2 cut(s) 74, 263
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 8 cut(s) 73, 74, 140, 172, 262, 263, 335, 428
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 2 cut(s) 571, 643
BsoBI CYCGRG 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 165
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 4 cut(s) 339, 562, 714, 721
BspACI CCGC 5 cut(s) 52, 68, 118, 184, 210
BspANI GGCC 2 cut(s) 571, 643
BspLI GGNNCC 1 cut(s) 351
BspMAI CTGCAG 3 cut(s) 237, 337, 532
BspMI ACCTGC 2 cut(s) 145, 340
BspPI GGATC 3 cut(s) 347, 570, 729
BsrGI TGTACA 1 cut(s) 124
BsrI ACTGG 4 cut(s) 167, 564, 610, 693
BssECI CCNNGG 4 cut(s) 261, 262, 411, 422
BssMI GATC 4 cut(s) 339, 562, 714, 721
BssSI CACGAG 1 cut(s) 279
BssT1I CCWWGG 2 cut(s) 411, 422
Bst2BI CACGAG 1 cut(s) 279
Bst6I CTCTTC 3 cut(s) 305, 456, 697
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 3 cut(s) 142, 208, 645
BstF5I GGATG 4 cut(s) 223, 514, 625, 738
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 4 cut(s) 342, 565, 717, 724
BstMBI GATC 4 cut(s) 339, 562, 714, 721
BstNSI RCATGY 2 cut(s) 210, 670
BstSCI CCNGG 3 cut(s) 73, 261, 262
BstSFI CTRYAG 3 cut(s) 233, 333, 528
BstV1I GCAGC 3 cut(s) 36, 219, 303
BstV2I GAAGAC 1 cut(s) 69
BsuRI GGCC 2 cut(s) 571, 643
BtsCI GGATG 4 cut(s) 223, 514, 625, 738
BtsIMutI CAGTG 1 cut(s) 349
BveI ACCTGC 2 cut(s) 145, 340
Cac8I GCNNGC 3 cut(s) 142, 208, 645
CfoI GCGC 1 cut(s) 269
Cfr9I CCCGGG 1 cut(s) 262
Csp6I GTAC 3 cut(s) 125, 670, 694
CviAII CATG 2 cut(s) 207, 667
CviQI GTAC 3 cut(s) 125, 670, 694
DpnI GATC 4 cut(s) 341, 564, 716, 723
DpnII GATC 4 cut(s) 339, 562, 714, 721
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 3 cut(s) 305, 456, 697
EarI CTCTTC 3 cut(s) 305, 456, 697
Eco130I CCWWGG 2 cut(s) 411, 422
Eco57I CTGAAG 3 cut(s) 437, 658, 675
Eco88I CYCGRG 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 411, 422
ErhI CCWWGG 2 cut(s) 411, 422
FaeI CATG 2 cut(s) 210, 670
FaqI GGGAC 1 cut(s) 598
FatI CATG 2 cut(s) 206, 666
FauI CCCGC 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 50, 119, 185, 233, 317
FokI GGATG 3 cut(s) 230, 501, 612
Fsp4HI GCNGC 5 cut(s) 50, 119, 185, 233, 317
FspBI CTAG 1 cut(s) 742
GlaI GCGC 1 cut(s) 268
GluI GCNGC 5 cut(s) 50, 119, 185, 233, 317
GsuI CTGGAG 1 cut(s) 710
HaeIII GGCC 2 cut(s) 571, 643
HapII CCGG 2 cut(s) 74, 263
HhaI GCGC 1 cut(s) 269
Hin1II CATG 2 cut(s) 210, 670
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HincII GTYRAC 2 cut(s) 9, 447
HindII GTYRAC 2 cut(s) 9, 447
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 4 cut(s) 10, 25, 416, 448
HpaII CCGG 2 cut(s) 74, 263
Hpy166II GTNNAC 2 cut(s) 9, 447
Hpy188I TCNGA 3 cut(s) 250, 655, 726
Hpy188III TCNNGA 1 cut(s) 22
Hpy8I GTNNAC 2 cut(s) 9, 447
HpyAV CCTTC 2 cut(s) 24, 582
HpyCH4V TGCA 8 cut(s) 206, 227, 235, 284, 335, 437, 530, 647
Hsp92II CATG 2 cut(s) 210, 670
HspAI GCGC 1 cut(s) 267
Kzo9I GATC 4 cut(s) 339, 562, 714, 721
LmnI GCTCC 2 cut(s) 168, 406
Lsp1109I GCAGC 3 cut(s) 36, 219, 303
MaeI CTAG 1 cut(s) 742
MalI GATC 4 cut(s) 341, 564, 716, 723
MboI GATC 4 cut(s) 339, 562, 714, 721
MboII GAAGA 6 cut(s) 71, 74, 322, 443, 602, 714
MfeI CAATTG 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 4 cut(s) 367, 537, 553, 649
MlyI GAGTC 3 cut(s) 4, 19, 442
MmeI TCCRAC 3 cut(s) 21, 228, 710
MnlI CCTC 9 cut(s) 27, 81, 208, 306, 414, 421, 425, 457, 702
MseI TTAA 3 cut(s) 300, 441, 549
MspI CCGG 2 cut(s) 74, 263
MspR9I CCNGG 3 cut(s) 75, 263, 264
MunI CAATTG 1 cut(s) 367
NciI CCSGG 3 cut(s) 75, 263, 264
NdeII GATC 4 cut(s) 339, 562, 714, 721
NlaIII CATG 2 cut(s) 210, 670
NlaIV GGNNCC 1 cut(s) 351
NspI RCATGY 2 cut(s) 210, 670
PaeI GCATGC 1 cut(s) 210
PaqCI CACCTGC 2 cut(s) 145, 340
PciI ACATGT 1 cut(s) 666
PfeI GAWTC 1 cut(s) 416
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 140
PkrI GCNGC 5 cut(s) 51, 120, 186, 234, 318
PleI GAGTC 3 cut(s) 4, 19, 442
PpsI GAGTC 3 cut(s) 4, 19, 442
PscI ACATGT 1 cut(s) 666
PspN4I GGNNCC 1 cut(s) 351
PstI CTGCAG 3 cut(s) 237, 337, 532
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 3 cut(s) 126, 671, 695
RsaNI GTAC 3 cut(s) 125, 670, 694
SaqAI TTAA 3 cut(s) 300, 441, 549
SatI GCNGC 5 cut(s) 50, 119, 185, 233, 317
Sau3AI GATC 4 cut(s) 339, 562, 714, 721
SbfI CCTGCAGG 1 cut(s) 337
SchI GAGTC 3 cut(s) 4, 19, 442
ScrFI CCNGG 3 cut(s) 75, 263, 264
SdaI CCTGCAGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 165
SetI ASST 9 cut(s) 103, 139, 182, 246, 290, 334, 403, 436, 661
SfcI CTRYAG 3 cut(s) 233, 333, 528
SmaI CCCGGG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
SphI GCATGC 1 cut(s) 210
Sse8387I CCTGCAGG 1 cut(s) 337
Sse9I AATT 4 cut(s) 367, 537, 553, 649
SsiI CCGC 5 cut(s) 52, 68, 118, 184, 210
SspMI CTAG 1 cut(s) 742
StyD4I CCNGG 3 cut(s) 73, 261, 262
StyI CCWWGG 2 cut(s) 411, 422
TaqI TCGA 1 cut(s) 32
TasI AATT 4 cut(s) 367, 537, 553, 649
TatI WGTACW 3 cut(s) 124, 669, 693
TauI GCSGC 2 cut(s) 121, 187
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 3 cut(s) 300, 441, 549
Tru9I TTAA 3 cut(s) 300, 441, 549
TscAI CASTG 1 cut(s) 349
TseI GCWGC 3 cut(s) 49, 232, 316
TspDTI ATGAA 1 cut(s) 503
TspGWI ACGGA 1 cut(s) 161
TspMI CCCGGG 1 cut(s) 262
TspRI CASTG 1 cut(s) 349
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 649
XceI RCATGY 2 cut(s) 210, 670
XmaI CCCGGG 1 cut(s) 262
XspI CTAG 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.