FvH4_6g02830

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001877
Physical Location & Seq
Forward (+)
1603035 .. 1605217
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02830.t4

Sequence Viewer

Length: 744 bp
ATGATGTTGACTCCTTCGATCAAGACTCTGTCGAGGGACTTAGATATGATGCGGATTGAGGAAGACCTGCCTCCGGGCGCAGAGATGAGTTTGGTGCTACCTGACTTGGACAGATTGAGGCTTGTACAGAACTCATCACCTGCAGGCGCGGAGAATAGTATGGTGCTTCCACGTTTGGACAGATTGCGGCTTGTAGAGCAATCAACTGGAGGCGGAGAGGATGTTTTTGCAAGGCTGCTAAAGGTGTCAGATTACCCACCCCCAGGCGCAGATTATACTCTTACAAGGTATAGACTGTTTAAAGAGTTGAAGAGGCTGCTACAAGACTCGCCTTCAGGGATCAGTGTGGTTCCTCCAGAATTCTACAATTGCAATATAATGTGTTGGAATGTTGTTATAGCTCCCCATTCTTTGGATTCTCCTTGGGAGGGAGGTGCATTTAAGTTGACTCTTCAGTTTAGCGAGGATTATCCACTCAAACCACCAACGGTGCGATTTGTTTGTCAGATGTTTCATCCAAATATTTCTGCAGATGGAACTATGTCTTTTGATAATTTACTGGATCGGTGGCCTCCTATAAATGATGTGGAAGATACACTCAAGTATATCCAGTCCCTAATATGTCATCCCAACCCAAAGTATCCTGCAAATTCAGAAGCTTTCTGCATGTACTTCAGTGACAACAGACTTGAGTACAACCGAAGAGTGAGGAGGATCGTGGATCAGAGTTGGATGGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

248

Amino Acids

28.29

Weight (kDa)

5.09

Isoelectric Point (pI)

62.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 98 - 239 1.2e-33 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 148
Acc36I ACCTGC 2 cut(s) 75, 148
AccB7I CCANNNNNTGG 1 cut(s) 412
AccII CGCG 1 cut(s) 149
AciI CCGC 4 cut(s) 52, 149, 187, 213
AclWI GGATC 4 cut(s) 347, 570, 722, 729
AcsI RAATTY 2 cut(s) 359, 649
AcuI CTGAAG 3 cut(s) 318, 437, 658
AfaI GTAC 3 cut(s) 126, 671, 695
AfiI CCNNNNNNNGG 4 cut(s) 73, 263, 412, 428
AgsI TTSAA 1 cut(s) 310
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 2 cut(s) 401, 659
AluI AGCT 2 cut(s) 401, 659
AlwI GGATC 4 cut(s) 347, 570, 722, 729
AoxI GGCC 1 cut(s) 569
ApeKI GCWGC 2 cut(s) 235, 316
ApoI RAATTY 2 cut(s) 359, 649
AspLEI GCGC 3 cut(s) 80, 149, 269
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 222, 303
BccI CCATC 2 cut(s) 527, 727
BciT130I CCWGG 1 cut(s) 264
BciVI GTATCC 1 cut(s) 651
BcnI CCSGG 1 cut(s) 75
BfaI CTAG 1 cut(s) 742
BfmI CTRYAG 2 cut(s) 141, 528
BfuAI ACCTGC 2 cut(s) 75, 148
BfuI GTATCC 1 cut(s) 651
BisI GCNGC 3 cut(s) 188, 236, 317
BlsI GCNGC 3 cut(s) 189, 237, 318
Bme1390I CCNGG 2 cut(s) 75, 264
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 75, 264
BmsI GCATC 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 2 cut(s) 228, 339
BpuEI CTTGAG 2 cut(s) 584, 710
BpuMI CCSGG 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 262, 422
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 263, 412, 428
Bse1I ACTGG 3 cut(s) 211, 564, 610
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 262, 422
BseGI GGATG 4 cut(s) 226, 514, 625, 738
BseLI CCNNNNNNNGG 4 cut(s) 73, 263, 412, 428
BseNI ACTGG 3 cut(s) 211, 564, 610
BseRI GAGGAG 1 cut(s) 724
BseXI GCAGC 2 cut(s) 222, 303
Bsh1236I CGCG 1 cut(s) 149
BshFI GGCC 1 cut(s) 571
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 2 cut(s) 50, 598
BslI CCNNNNNNNGG 4 cut(s) 73, 263, 412, 428
BsmFI GGGAC 2 cut(s) 50, 598
BsnI GGCC 1 cut(s) 571
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 5 cut(s) 18, 339, 562, 714, 721
BspACI CCGC 4 cut(s) 52, 149, 187, 213
BspANI GGCC 1 cut(s) 571
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 1 cut(s) 351
BspMAI CTGCAG 2 cut(s) 145, 532
BspMI ACCTGC 2 cut(s) 75, 148
BspPI GGATC 4 cut(s) 347, 570, 722, 729
BsrGI TGTACA 1 cut(s) 124
BsrI ACTGG 3 cut(s) 211, 564, 610
BssECI CCNNGG 2 cut(s) 262, 422
BssMI GATC 5 cut(s) 18, 339, 562, 714, 721
BssT1I CCWWGG 1 cut(s) 422
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 297, 490
Bst6I CTCTTC 3 cut(s) 305, 456, 697
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 1 cut(s) 40
BstF5I GGATG 4 cut(s) 226, 514, 625, 738
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 3 cut(s) 80, 149, 269
BstKTI GATC 5 cut(s) 21, 342, 565, 717, 724
BstMBI GATC 5 cut(s) 18, 339, 562, 714, 721
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 2 cut(s) 73, 262
BstSFI CTRYAG 2 cut(s) 141, 528
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 2 cut(s) 222, 303
BstV2I GAAGAC 1 cut(s) 69
BsuI GTATCC 1 cut(s) 651
BsuRI GGCC 1 cut(s) 571
BtsCI GGATG 4 cut(s) 226, 514, 625, 738
BtsIMutI CAGTG 2 cut(s) 349, 682
BveI ACCTGC 2 cut(s) 75, 148
Cac8I GCNNGC 1 cut(s) 145
CfoI GCGC 3 cut(s) 80, 149, 269
Csp6I GTAC 3 cut(s) 125, 670, 694
CviAII CATG 1 cut(s) 667
CviJI RGCY 7 cut(s) 121, 190, 235, 316, 401, 571, 659
CviKI_1 RGCY 7 cut(s) 121, 190, 235, 316, 401, 571, 659
CviQI GTAC 3 cut(s) 125, 670, 694
DdeI CTNAG 1 cut(s) 40
DpnI GATC 5 cut(s) 20, 341, 564, 716, 723
DpnII GATC 5 cut(s) 18, 339, 562, 714, 721
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 3 cut(s) 305, 456, 697
EarI CTCTTC 3 cut(s) 305, 456, 697
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 422
Eco57I CTGAAG 3 cut(s) 318, 437, 658
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 1 cut(s) 670
FaqI GGGAC 2 cut(s) 50, 598
FatI CATG 1 cut(s) 666
Fnu4HI GCNGC 3 cut(s) 188, 236, 317
FokI GGATG 3 cut(s) 233, 501, 612
Fsp4HI GCNGC 3 cut(s) 188, 236, 317
FspBI CTAG 1 cut(s) 742
GlaI GCGC 3 cut(s) 79, 148, 268
GluI GCNGC 3 cut(s) 188, 236, 317
GsuI CTGGAG 2 cut(s) 228, 339
HaeIII GGCC 1 cut(s) 571
HapII CCGG 1 cut(s) 74
HhaI GCGC 3 cut(s) 80, 149, 269
Hin1II CATG 1 cut(s) 670
Hin6I GCGC 3 cut(s) 78, 147, 267
HinP1I GCGC 3 cut(s) 78, 147, 267
HincII GTYRAC 2 cut(s) 9, 447
HindII GTYRAC 2 cut(s) 9, 447
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 5 cut(s) 10, 25, 326, 416, 448
HpaII CCGG 1 cut(s) 74
HphI GGTGA 1 cut(s) 129
Hpy166II GTNNAC 2 cut(s) 9, 447
Hpy188I TCNGA 4 cut(s) 250, 507, 655, 726
Hpy188III TCNNGA 2 cut(s) 22, 356
Hpy8I GTNNAC 2 cut(s) 9, 447
HpyAV CCTTC 2 cut(s) 24, 342
HpyCH4III ACNGT 2 cut(s) 297, 490
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 7 cut(s) 143, 230, 372, 437, 530, 647, 666
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 1 cut(s) 40
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 1 cut(s) 670
HspAI GCGC 3 cut(s) 78, 147, 267
Kzo9I GATC 5 cut(s) 18, 339, 562, 714, 721
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 2 cut(s) 222, 303
LweI GCATC 1 cut(s) 39
MaeI CTAG 1 cut(s) 742
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 1 cut(s) 677
MalI GATC 5 cut(s) 20, 341, 564, 716, 723
MboI GATC 5 cut(s) 18, 339, 562, 714, 721
MboII GAAGA 5 cut(s) 74, 322, 443, 602, 714
MfeI CAATTG 1 cut(s) 367
MluCI AATT 4 cut(s) 359, 367, 553, 649
MlyI GAGTC 4 cut(s) 4, 19, 320, 442
MmeI TCCRAC 2 cut(s) 365, 710
MseI TTAA 2 cut(s) 300, 441
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 2 cut(s) 75, 264
MunI CAATTG 1 cut(s) 367
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 1 cut(s) 196
NciI CCSGG 1 cut(s) 75
NdeII GATC 5 cut(s) 18, 339, 562, 714, 721
NlaIII CATG 1 cut(s) 670
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 677
NspI RCATGY 1 cut(s) 670
PaqCI CACCTGC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 416
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 412
PkrI GCNGC 3 cut(s) 189, 237, 318
PleI GAGTC 4 cut(s) 4, 19, 320, 442
PpsI GAGTC 4 cut(s) 4, 19, 320, 442
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 351
PstI CTGCAG 2 cut(s) 145, 532
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 3 cut(s) 126, 671, 695
RsaNI GTAC 3 cut(s) 125, 670, 694
SaqAI TTAA 2 cut(s) 300, 441
SatI GCNGC 3 cut(s) 188, 236, 317
Sau3AI GATC 5 cut(s) 18, 339, 562, 714, 721
SbfI CCTGCAGG 1 cut(s) 145
SchI GAGTC 4 cut(s) 4, 19, 320, 442
ScrFI CCNGG 2 cut(s) 75, 264
SdaI CCTGCAGG 1 cut(s) 145
SetI ASST 9 cut(s) 69, 103, 142, 175, 246, 290, 403, 436, 661
SfaNI GCATC 1 cut(s) 39
SfcI CTRYAG 2 cut(s) 141, 528
SmlI CTYRAG 2 cut(s) 599, 689
SmoI CTYRAG 2 cut(s) 599, 689
Sse8387I CCTGCAGG 1 cut(s) 145
Sse9I AATT 4 cut(s) 359, 367, 553, 649
SsiI CCGC 4 cut(s) 52, 149, 187, 213
SspI AATATT 1 cut(s) 523
SspMI CTAG 1 cut(s) 742
StyD4I CCNGG 2 cut(s) 73, 262
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 2 cut(s) 297, 490
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 17, 32
TasI AATT 4 cut(s) 359, 367, 553, 649
TatI WGTACW 3 cut(s) 124, 669, 693
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 2 cut(s) 300, 441
Tru9I TTAA 2 cut(s) 300, 441
TscAI CASTG 2 cut(s) 349, 682
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 2 cut(s) 235, 316
Tsp45I GTSAC 1 cut(s) 677
TspDTI ATGAA 1 cut(s) 503
TspRI CASTG 2 cut(s) 349, 682
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 412
XapI RAATTY 2 cut(s) 359, 649
XceI RCATGY 1 cut(s) 670
XspI CTAG 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.