Rroxscaffold_6G00428420

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001877
Physical Location & Seq
Forward (+)
48817672 .. 48818796
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428420.1

Sequence Viewer

Length: 507 bp
ATGGCCCCTTCATACTTGGATAGGCTGCAGAAAAAAGTGTCGGATGACCCACCCCCGGGCGCAGATTATACTCTTGCAAGGTATAGGTTGTGTAAAGAGTTGAAGAGGCTGCTACAAGACCCACCTGCAGGGATCGGTGTGGTTCCCCCGGACTTCTACAATTGCGATATAATGTTGTGGAATGTTGTTATAGCTCCTCATTCCATGGATTCTCCTTGGGAGGGAGGCGCATGCACATTTAAGTTGACTCTTCAGTTCAGTGAGGATTATCCGCTCAAACCATCAATGGTGCGACTTGTTTCTCAAATGTTTCATGACTTCTTCCAAACATTTCTGCAGATGGAATCCCTAATATGTCATCCCAACCCGAAGTGCATGTCCTGCAAATTCAGAAGCTTTCTACATGTATTTCAGCGAGAACAGACTCGAGTACAACCGAAGAGTGAGGATGATTGTGGATCAGAGTTGGATGGCAGGCTAGTAACTTCTTTATGTTACACTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

168

Amino Acids

19.32

Weight (kDa)

6.06

Isoelectric Point (pI)

59.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 29 - 105 3.7e-11 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
Acc36I ACCTGC 1 cut(s) 133
AccBSI CCGCTC 1 cut(s) 274
AciI CCGC 1 cut(s) 272
AclWI GGATC 2 cut(s) 140, 466
AcsI RAATTY 1 cut(s) 386
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 1 cut(s) 103
AluBI AGCT 2 cut(s) 194, 396
AluI AGCT 2 cut(s) 194, 396
AlwI GGATC 2 cut(s) 140, 466
Ama87I CYCGRG 2 cut(s) 55, 426
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 25, 109
ApoI RAATTY 1 cut(s) 386
ArsI GACNNNNNNTTYG 2 cut(s) 362, 394
AspLEI GCGC 2 cut(s) 62, 230
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 3 cut(s) 56, 57, 149
AvaI CYCGRG 2 cut(s) 55, 426
BbvI GCAGC 2 cut(s) 12, 96
BccI CCATC 3 cut(s) 289, 334, 464
BcnI CCSGG 3 cut(s) 56, 57, 149
BfaI CTAG 1 cut(s) 479
BfmI CTRYAG 3 cut(s) 26, 126, 335
BfuAI ACCTGC 1 cut(s) 133
BisI GCNGC 2 cut(s) 26, 110
BlsI GCNGC 2 cut(s) 27, 111
Bme1390I CCNGG 3 cut(s) 56, 57, 149
BmeT110I CYCGRG 2 cut(s) 55, 426
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 144
BmrFI CCNGG 3 cut(s) 56, 57, 149
BpuMI CCSGG 3 cut(s) 56, 57, 149
BsaJI CCNNGG 5 cut(s) 54, 55, 147, 204, 215
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
BseDI CCNNGG 5 cut(s) 54, 55, 147, 204, 215
BseGI GGATG 4 cut(s) 49, 358, 454, 475
BseLI CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 2 cut(s) 12, 96
BshFI GGCC 1 cut(s) 5
BsiHKCI CYCGRG 2 cut(s) 55, 426
BsiSI CCGG 2 cut(s) 56, 149
BslI CCNNNNNNNGG 4 cut(s) 55, 56, 128, 221
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 2 cut(s) 55, 426
Bsp143I GATC 2 cut(s) 132, 458
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 1 cut(s) 272
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 6, 144
BspMAI CTGCAG 3 cut(s) 30, 130, 339
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 2 cut(s) 140, 466
BsrBI CCGCTC 1 cut(s) 274
BssECI CCNNGG 5 cut(s) 54, 55, 147, 204, 215
BssMI GATC 2 cut(s) 132, 458
BssT1I CCWWGG 2 cut(s) 204, 215
Bst6I CTCTTC 3 cut(s) 98, 255, 434
BstAPI GCANNNNNTGC 1 cut(s) 381
BstC8I GCNNGC 2 cut(s) 232, 476
BstDEI CTNAG 1 cut(s) 501
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 4 cut(s) 49, 358, 454, 475
BstHHI GCGC 2 cut(s) 62, 230
BstKTI GATC 2 cut(s) 135, 461
BstMBI GATC 2 cut(s) 132, 458
BstMWI GCNNNNNNNGC 1 cut(s) 381
BstNSI RCATGY 3 cut(s) 234, 379, 407
BstSCI CCNGG 3 cut(s) 54, 55, 147
BstSFI CTRYAG 3 cut(s) 26, 126, 335
BstV1I GCAGC 2 cut(s) 12, 96
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 4 cut(s) 49, 358, 454, 475
BtsIMutI CAGTG 1 cut(s) 265
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 232, 476
CciI TCATGA 1 cut(s) 313
CfoI GCGC 2 cut(s) 62, 230
Cfr13I GGNCC 1 cut(s) 4
Cfr9I CCCGGG 1 cut(s) 55
Csp6I GTAC 1 cut(s) 431
CviAII CATG 5 cut(s) 205, 231, 314, 376, 404
CviJI RGCY 6 cut(s) 5, 25, 109, 194, 396, 478
CviKI_1 RGCY 6 cut(s) 5, 25, 109, 194, 396, 478
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 1 cut(s) 501
DpnI GATC 2 cut(s) 134, 460
DpnII GATC 2 cut(s) 132, 458
Eam1104I CTCTTC 3 cut(s) 98, 255, 434
EarI CTCTTC 3 cut(s) 98, 255, 434
Eco130I CCWWGG 2 cut(s) 204, 215
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 2 cut(s) 55, 426
EcoT14I CCWWGG 2 cut(s) 204, 215
ErhI CCWWGG 2 cut(s) 204, 215
FaeI CATG 5 cut(s) 208, 234, 317, 379, 407
FatI CATG 5 cut(s) 204, 230, 313, 375, 403
Fnu4HI GCNGC 2 cut(s) 26, 110
FokI GGATG 4 cut(s) 56, 345, 461, 482
Fsp4HI GCNGC 2 cut(s) 26, 110
FspBI CTAG 1 cut(s) 479
GlaI GCGC 2 cut(s) 61, 229
GluI GCNGC 2 cut(s) 26, 110
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 56, 149
HhaI GCGC 2 cut(s) 62, 230
Hin1II CATG 5 cut(s) 208, 234, 317, 379, 407
Hin6I GCGC 2 cut(s) 60, 228
HinP1I GCGC 2 cut(s) 60, 228
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 4 cut(s) 209, 247, 344, 424
HpaII CCGG 2 cut(s) 56, 149
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 3 cut(s) 43, 392, 463
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 1 cut(s) 18
HpyCH4V TGCA 7 cut(s) 28, 77, 128, 234, 337, 375, 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 381
HpyF3I CTNAG 1 cut(s) 501
Hsp92II CATG 5 cut(s) 208, 234, 317, 379, 407
HspAI GCGC 2 cut(s) 60, 228
Kzo9I GATC 2 cut(s) 132, 458
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 6 cut(s) 69, 114, 138, 162, 394, 460
Lsp1109I GCAGC 2 cut(s) 12, 96
MaeI CTAG 1 cut(s) 479
MaeIII GTNAC 2 cut(s) 481, 494
MalI GATC 2 cut(s) 134, 460
MbiI CCGCTC 1 cut(s) 274
MboI GATC 2 cut(s) 132, 458
MboII GAAGA 4 cut(s) 115, 242, 313, 451
MfeI CAATTG 1 cut(s) 160
MluCI AATT 2 cut(s) 160, 386
MlyI GAGTC 2 cut(s) 241, 418
MmeI TCCRAC 2 cut(s) 21, 447
MnlI CCTC 6 cut(s) 99, 207, 214, 218, 256, 439
MseI TTAA 1 cut(s) 240
MspI CCGG 2 cut(s) 56, 149
MspR9I CCNGG 3 cut(s) 56, 57, 149
MunI CAATTG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 381
NciI CCSGG 3 cut(s) 56, 57, 149
NcoI CCATGG 1 cut(s) 204
NdeII GATC 2 cut(s) 132, 458
NlaIII CATG 5 cut(s) 208, 234, 317, 379, 407
NlaIV GGNNCC 2 cut(s) 6, 144
NspI RCATGY 3 cut(s) 234, 379, 407
PaeI GCATGC 1 cut(s) 234
PaeR7I CTCGAG 1 cut(s) 426
PagI TCATGA 1 cut(s) 313
PaqCI CACCTGC 1 cut(s) 133
PciI ACATGT 1 cut(s) 403
PfeI GAWTC 2 cut(s) 209, 344
PkrI GCNGC 2 cut(s) 27, 111
PleI GAGTC 2 cut(s) 241, 418
PpsI GAGTC 2 cut(s) 241, 418
PscI ACATGT 1 cut(s) 403
PspN4I GGNNCC 2 cut(s) 6, 144
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 426
PstI CTGCAG 3 cut(s) 30, 130, 339
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 2 cut(s) 26, 110
Sau3AI GATC 2 cut(s) 132, 458
Sau96I GGNCC 1 cut(s) 4
SbfI CCTGCAGG 1 cut(s) 130
SchI GAGTC 2 cut(s) 241, 418
ScrFI CCNGG 3 cut(s) 56, 57, 149
SdaI CCTGCAGG 1 cut(s) 130
SetI ASST 5 cut(s) 83, 89, 127, 196, 398
SfcI CTRYAG 3 cut(s) 26, 126, 335
Sfr274I CTCGAG 1 cut(s) 426
SlaI CTCGAG 1 cut(s) 426
SmaI CCCGGG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
SphI GCATGC 1 cut(s) 234
Sse8387I CCTGCAGG 1 cut(s) 130
Sse9I AATT 2 cut(s) 160, 386
SsiI CCGC 1 cut(s) 272
SspMI CTAG 1 cut(s) 479
StyD4I CCNGG 3 cut(s) 54, 55, 147
StyI CCWWGG 2 cut(s) 204, 215
TaqI TCGA 1 cut(s) 427
TasI AATT 2 cut(s) 160, 386
TatI WGTACW 1 cut(s) 430
TfiI GAWTC 2 cut(s) 209, 344
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 265
TseI GCWGC 2 cut(s) 25, 109
TspDTI ATGAA 1 cut(s) 302
TspMI CCCGGG 1 cut(s) 55
TspRI CASTG 1 cut(s) 265
XapI RAATTY 1 cut(s) 386
XceI RCATGY 3 cut(s) 234, 379, 407
XhoI CTCGAG 1 cut(s) 426
XmaI CCCGGG 1 cut(s) 55
XspI CTAG 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.