FvH4_5g21160

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003066
Physical Location & Seq
Forward (+)
12709861 .. 12713233
3373 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21160.t1

Sequence Viewer

Length: 666 bp
ATGACAGTAGACAGCGCCACCATGGAAGACATGGAAGCCAAGGCCGACCAGCTCGGTATCCAGTTCTCCTCCATAGACTTGAATTCTTCACACCCACCTCACTTTAATTTCAGTGAATGCCAAGAAGTGTTTTACGATGAAAATGGTTTCATTCCCAATATTGAGTTTGGGGGTGTGATTGTGGTTAGTAATCTTCCTGTTGTTGAGGCAGAGATGGCCCAGGAGCTTGAAAGCGAGATTCGAGTGGTGTTTAGTGAAGTTGGTGTTATTAAAGAAGATGGGTTTTCGATGCCTGTGAACCCCACCACCCAAGAAACCTTGGGTCATTGTTTCATTGAGTTTAATTCTCAGGAGGAAGCCGCACTTGCTAGGAACAAGAGAGATGGATACAAGTTTGGTGATTGCAAGATGTCAGTAAGCCTCTATGATGCCTTTGGCAGTTTAGCTGCTATTCCTGGCACGGGGACTTATTCAGAGATTAATGCATCCTTTTGTGGGTCCTCAAGGTATCCAGGGTCGCCTTCTTTGCTTAGTCCTGTAACGGAGGAAGACGAAGAACCCGTTTATAGTGATGTTTCTACTGCAGATGACAACGATGAAATAATGATGTGGAGTGAGCAAGAAAACGATATGAGCAGTGAGGAATTTAGGGAAGTCATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

222

Amino Acids

24.43

Weight (kDa)

4.05

Isoelectric Point (pI)

66.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 77 - 137 1.3e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AciI CCGC 1 cut(s) 360
AcsI RAATTY 2 cut(s) 82, 644
AfiI CCNNNNNNNGG 2 cut(s) 461, 495
AgsI TTSAA 3 cut(s) 82, 230, 662
AjnI CCWGG 3 cut(s) 219, 454, 511
AluBI AGCT 3 cut(s) 52, 226, 446
AluI AGCT 3 cut(s) 52, 226, 446
AoxI GGCC 2 cut(s) 42, 216
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 2 cut(s) 82, 644
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 2 cut(s) 217, 498
AsuHPI GGTGA 1 cut(s) 410
AvaII GGWCC 1 cut(s) 498
BbsI GAAGAC 2 cut(s) 33, 555
BbvI GCAGC 1 cut(s) 433
BccI CCATC 3 cut(s) 208, 272, 377
BciT130I CCWGG 3 cut(s) 221, 456, 513
BciVI GTATCC 3 cut(s) 68, 380, 519
BfaI CTAG 1 cut(s) 369
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 1 cut(s) 18
BfuI GTATCC 3 cut(s) 68, 380, 519
BisI GCNGC 2 cut(s) 360, 447
BlsI GCNGC 2 cut(s) 361, 448
Bme1390I CCNGG 3 cut(s) 221, 456, 513
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 217, 498
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 3 cut(s) 221, 456, 513
BmsI GCATC 3 cut(s) 279, 418, 494
BpiI GAAGAC 2 cut(s) 33, 555
BpuEI CTTGAG 1 cut(s) 487
BsaJI CCNNGG 5 cut(s) 21, 39, 219, 318, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 495
Bse1I ACTGG 1 cut(s) 61
BseBI CCWGG 3 cut(s) 221, 456, 513
BseDI CCNNGG 5 cut(s) 21, 39, 219, 318, 512
BseGI GGATG 1 cut(s) 485
BseLI CCNNNNNNNGG 2 cut(s) 461, 495
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 433
BshFI GGCC 2 cut(s) 44, 218
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 2 cut(s) 461, 495
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 2 cut(s) 44, 218
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 1 cut(s) 360
BspANI GGCC 2 cut(s) 44, 218
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 499
BspMAI CTGCAG 1 cut(s) 586
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 5 cut(s) 21, 39, 219, 318, 512
BssT1I CCWWGG 3 cut(s) 21, 39, 318
Bst2UI CCWGG 3 cut(s) 221, 456, 513
Bst4CI ACNGT 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 348, 530
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 485
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstMWI GCNNNNNNNGC 3 cut(s) 215, 365, 526
BstNI CCWGG 3 cut(s) 221, 456, 513
BstSCI CCNGG 3 cut(s) 219, 454, 511
BstSFI CTRYAG 1 cut(s) 582
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 2 cut(s) 33, 555
BsuI GTATCC 3 cut(s) 68, 380, 519
BsuRI GGCC 2 cut(s) 44, 218
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 485
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 2 cut(s) 118, 643
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 217, 498
CviAII CATG 2 cut(s) 22, 31
CviJI RGCY 8 cut(s) 38, 44, 52, 218, 226, 359, 420, 446
CviKI_1 RGCY 8 cut(s) 38, 44, 52, 218, 226, 359, 420, 446
DdeI CTNAG 2 cut(s) 348, 530
Eco130I CCWWGG 3 cut(s) 21, 39, 318
Eco47I GGWCC 1 cut(s) 498
EcoO109I RGGNCCY 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 219, 454, 511
EcoT14I CCWWGG 3 cut(s) 21, 39, 318
EcoT22I ATGCAT 1 cut(s) 487
ErhI CCWWGG 3 cut(s) 21, 39, 318
FaeI CATG 2 cut(s) 25, 34
FaiI YATR 6 cut(s) 23, 32, 74, 426, 567, 632
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 478
FatI CATG 2 cut(s) 21, 30
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 360, 447
FokI GGATG 1 cut(s) 472
Fsp4HI GCNGC 2 cut(s) 360, 447
FspBI CTAG 1 cut(s) 369
GlaI GCGC 1 cut(s) 16
GluI GCNGC 2 cut(s) 360, 447
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 2 cut(s) 44, 218
HhaI GCGC 1 cut(s) 17
Hin1II CATG 2 cut(s) 25, 34
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HinfI GANTC 1 cut(s) 238
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 10, 298
Hpy188I TCNGA 1 cut(s) 475
Hpy188III TCNNGA 1 cut(s) 350
Hpy8I GTNNAC 2 cut(s) 10, 298
HpyAV CCTTC 1 cut(s) 531
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4V TGCA 3 cut(s) 405, 485, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 365, 526
HpyF3I CTNAG 2 cut(s) 348, 530
Hsp92II CATG 2 cut(s) 25, 34
HspAI GCGC 1 cut(s) 15
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 3 cut(s) 279, 418, 494
MaeI CTAG 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 538
MboII GAAGA 6 cut(s) 38, 78, 185, 287, 560, 566
MluCI AATT 4 cut(s) 82, 106, 343, 644
MnlI CCTC 8 cut(s) 79, 108, 199, 346, 431, 511, 538, 634
Mph1103I ATGCAT 1 cut(s) 487
MseI TTAA 4 cut(s) 105, 270, 342, 480
MspR9I CCNGG 3 cut(s) 221, 456, 513
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 3 cut(s) 221, 456, 513
MwoI GCNNNNNNNGC 3 cut(s) 215, 365, 526
NcoI CCATGG 1 cut(s) 21
NlaIII CATG 2 cut(s) 25, 34
NlaIV GGNNCC 1 cut(s) 499
NsiI ATGCAT 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 2 cut(s) 361, 448
PpuMI RGGWCCY 1 cut(s) 498
PshBI ATTAAT 1 cut(s) 480
Psp5II RGGWCCY 1 cut(s) 498
Psp6I CCWGG 3 cut(s) 219, 454, 511
PspGI CCWGG 3 cut(s) 219, 454, 511
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 2 cut(s) 217, 498
PspPPI RGGWCCY 1 cut(s) 498
PstI CTGCAG 1 cut(s) 586
SaqAI TTAA 4 cut(s) 105, 270, 342, 480
SatI GCNGC 2 cut(s) 360, 447
Sau96I GGNCC 2 cut(s) 217, 498
ScrFI CCNGG 3 cut(s) 221, 456, 513
SetI ASST 6 cut(s) 54, 100, 228, 320, 448, 509
SfaNI GCATC 3 cut(s) 279, 418, 494
SfcI CTRYAG 1 cut(s) 582
SinI GGWCC 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
Sse9I AATT 4 cut(s) 82, 106, 343, 644
SsiI CCGC 1 cut(s) 360
SspI AATATT 1 cut(s) 160
SspMI CTAG 1 cut(s) 369
StyD4I CCNGG 3 cut(s) 219, 454, 511
StyI CCWWGG 3 cut(s) 21, 39, 318
TaaI ACNGT 1 cut(s) 7
TaqI TCGA 2 cut(s) 241, 287
TasI AATT 4 cut(s) 82, 106, 343, 644
TauI GCSGC 1 cut(s) 362
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 4 cut(s) 105, 270, 342, 480
Tru9I TTAA 4 cut(s) 105, 270, 342, 480
TscAI CASTG 2 cut(s) 118, 643
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 4 cut(s) 139, 153, 322, 612
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 2 cut(s) 118, 643
VpaK11BI GGWCC 1 cut(s) 498
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 2 cut(s) 82, 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 1 cut(s) 369
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.