Rorug07G0103000

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003066
Physical Location & Seq
Reverse (-)
8108432 .. 8112638
4207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0103000.1

Sequence Viewer

Length: 1419 bp
ATGGAGAAGAGAGAAAACGGCGTCGTAGAGCACATGCAGATCGACCGGTCGGCCCCTTCCTGCAACGAGACCTCTTTCTCTGCCCTATTCCATGAAAAACGAGGTTACCTTACTAATTCCTCTATTGCTCTTATTCTCTTTGCTGTTCACTACTTTAGCGAAATGAAGACTATTCCTTATTTTGATCCTCAAAATTACAATGCATTTAAGAATTGCTCCATTATAAGAAAATTCAGTGATTCTAAAATTGTTACTGAAGTTGCAAGATGCTTGGCCGATGCTGGAATCGTCTTTCGAAGAGTTTGGCGTTTCATGTGCACTGAATCTGCGTTCCCTGATAGTCTCAATACAACCGGAAGGACTAAGGGCCGGAATTCTTTTGATAAGGCTACACGCGTCGTTGAACTTTTGGCAACAACTCATGTTCCACCATATCTGGCAATAGAAATACTAAATGGTGATCGGAAACATATCCTCATCAAAACTGGGAATCAACAAGGTGGGCTTTGCTCCATATATGGGATCAAGAAGGATCAATTTGTTAAACAACGGGAATGTCTTGCCTGCTCCCTAGAGGCACTTGCAGCACTGACATTTTGTTATCTTTTTCTTAATGTAAGAAGACACTGTTGCTGCAGTGGGAGACCCTCTAGCTTTAATGGCAATCAGGAAGCTTGTGTCGTAGGCTGCATTGTTAACAATGCGGATCCTGCAACAACTATCAATGAGGTTAAAACTGTCTTGAGGAGGAGACAAGGAGTGGAAAATCTTCAACACCCAAATGTCGATTACTTGAGAATGGAGAACTCTGGCCCTTCCTTGGATCTATATGGCATGCTTGAAGTAACCTCTTTCTCTAAGACCTGCTATATAGGCCGAGAAAAAGTGGTGCAAGAAGCTTTGCCATTTTTGCAATCGTTTTTAGAAGAGTATGGCATTTCATGCACTCTGAATATGGCTGAGCGTTCCATGACACTTTCAACTACCAGAAGAACCAAGGATCCTGATGTTATGGACAAGGCTGTCATTCTTGTTGAGCTTCTGGCAAGAACTAGTATTCCGGCATGTAAGGCAATAGAAATCCTGCATGGCCGCCTGCAACATGAAGTCATCAAGACTGAGTTTCAAGAAGGTGGACTTTGCTCTAAATATGGAATCAAGAAGGAGCGATTTGTTAAAAACAAGGACCGATTCAAGTGCTACTTGCAGACACTTGCACGCCTGTCAAAGTGTGAGATTTACATTAATGAATCCAGTGTTGTTGCAGTGGGAACTTCAGGTGCAGGATTAAGGCGGTTCAGGTCAATTGTCGAGTGCTGCTTCATTAAAGATGAAGATCCCGCTACCATTGTTAGGAGGTTGAAATTGAATGGGGAAACCTACCGATTAATGAAGAAGCTTGAGGCTTTGTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

472

Amino Acids

53.38

Weight (kDa)

8.94

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_1st PF17903 293 - 362 4.5e-10 Krr1 KH1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 1022
Acc36I ACCTGC 1 cut(s) 872
AccII CGCG 1 cut(s) 396
AciI CCGC 4 cut(s) 704, 1093, 1294, 1341
AclWI GGATC 9 cut(s) 179, 530, 540, 701, 714, 831, 995, 1008, 1331
AcoI YGGCCR 2 cut(s) 273, 1090
AcsI RAATTY 2 cut(s) 230, 373
AcuI CTGAAG 2 cut(s) 276, 1260
AcyI GRCGYC 1 cut(s) 21
AfiI CCNNNNNNNGG 3 cut(s) 519, 820, 1353
AflIII ACRYGT 1 cut(s) 394
AgeI ACCGGT 1 cut(s) 45
AgsI TTSAA 8 cut(s) 404, 773, 842, 981, 1127, 1195, 1363, 1369
AhlI ACTAGT 1 cut(s) 1052
AloI GAACNNNNNNTCC 4 cut(s) 985, 1017, 1042, 1074
AluBI AGCT 5 cut(s) 654, 674, 899, 1039, 1399
AluI AGCT 5 cut(s) 654, 674, 899, 1039, 1399
Alw21I GWGCWC 2 cut(s) 33, 320
Alw26I GTCTC 4 cut(s) 62, 347, 637, 745
Alw44I GTGCAC 1 cut(s) 316
AlwI GGATC 9 cut(s) 179, 530, 540, 701, 714, 831, 995, 1008, 1331
AoxI GGCC 6 cut(s) 51, 273, 367, 811, 874, 1090
ApaLI GTGCAC 1 cut(s) 316
ApeKI GCWGC 4 cut(s) 584, 633, 687, 1317
ApoI RAATTY 2 cut(s) 230, 373
AseI ATTAAT 2 cut(s) 1245, 1388
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 1 cut(s) 768
AspS9I GGNCC 4 cut(s) 52, 367, 812, 1186
AsuHPI GGTGA 1 cut(s) 470
AsuII TTCGAA 1 cut(s) 295
AvaII GGWCC 1 cut(s) 1186
BaeGI GKGCMC 1 cut(s) 320
BamHI GGATCC 2 cut(s) 706, 1000
BbsI GAAGAC 2 cut(s) 173, 628
Bbv12I GWGCWC 2 cut(s) 33, 320
BbvI GCAGC 4 cut(s) 596, 620, 674, 1304
BceAI ACGGC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 1179, 1213
BcoDI GTCTC 4 cut(s) 62, 347, 637, 745
BcuI ACTAGT 1 cut(s) 1052
BfaI CTAG 3 cut(s) 572, 651, 1053
BfmI CTRYAG 1 cut(s) 634
BfuAI ACCTGC 1 cut(s) 872
BisI GCNGC 5 cut(s) 585, 634, 688, 1093, 1318
BlpI GCTNAGC 1 cut(s) 960
BlsI GCNGC 5 cut(s) 586, 635, 689, 1094, 1319
Bme18I GGWCC 1 cut(s) 1186
BmgT120I GGNCC 4 cut(s) 52, 367, 812, 1186
BmiI GGNNCC 3 cut(s) 54, 708, 1002
BmrI ACTGGG 1 cut(s) 495
BmsI GCATC 2 cut(s) 257, 268
BmuI ACTGGG 1 cut(s) 495
BpiI GAAGAC 2 cut(s) 173, 628
Bpu1102I GCTNAGC 1 cut(s) 960
Bpu14I TTCGAA 1 cut(s) 295
BpuEI CTTGAG 2 cut(s) 763, 814
BsaBI GATNNNNATC 1 cut(s) 1335
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 2 cut(s) 62, 637
BsaJI CCNNGG 2 cut(s) 819, 996
BsaWI WCCGGW 2 cut(s) 45, 353
Bsc4I CCNNNNNNNGG 3 cut(s) 519, 820, 1353
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 2 cut(s) 490, 1254
Bse8I GATNNNNATC 1 cut(s) 1335
BseDI CCNNGG 2 cut(s) 819, 996
BseJI GATNNNNATC 1 cut(s) 1335
BseLI CCNNNNNNNGG 3 cut(s) 519, 820, 1353
BseMII CTCAG 2 cut(s) 951, 1110
BseNI ACTGG 2 cut(s) 490, 1254
BseRI GAGGAG 2 cut(s) 760, 763
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 4 cut(s) 596, 620, 674, 1304
BsgI GTGCAG 1 cut(s) 1302
Bsh1236I CGCG 1 cut(s) 396
Bsh1285I CGRYCG 2 cut(s) 46, 50
BshFI GGCC 6 cut(s) 53, 275, 369, 813, 876, 1092
BshTI ACCGGT 1 cut(s) 45
BsiEI CGRYCG 2 cut(s) 46, 50
BsiHKAI GWGCWC 2 cut(s) 33, 320
BsiSI CCGG 4 cut(s) 46, 354, 370, 1061
BslI CCNNNNNNNGG 3 cut(s) 519, 820, 1353
BsmAI GTCTC 4 cut(s) 62, 347, 637, 745
BsnI GGCC 6 cut(s) 53, 275, 369, 813, 876, 1092
Bso31I GGTCTC 2 cut(s) 62, 637
Bsp119I TTCGAA 1 cut(s) 295
Bsp1286I GDGCHC 2 cut(s) 33, 320
Bsp143I GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
Bsp1720I GCTNAGC 1 cut(s) 960
BspACI CCGC 4 cut(s) 704, 1093, 1294, 1341
BspANI GGCC 6 cut(s) 53, 275, 369, 813, 876, 1092
BspCNI CTCAG 2 cut(s) 952, 1111
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 3 cut(s) 54, 708, 1002
BspMAI CTGCAG 1 cut(s) 638
BspMI ACCTGC 1 cut(s) 872
BspPI GGATC 9 cut(s) 179, 530, 540, 701, 714, 831, 995, 1008, 1331
BspT104I TTCGAA 1 cut(s) 295
BspTNI GGTCTC 2 cut(s) 62, 637
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 2 cut(s) 490, 1254
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 819, 996
BssMI GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 2 cut(s) 819, 996
Bst4CI ACNGT 2 cut(s) 629, 739
Bst6I CTCTTC 3 cut(s) 2, 292, 921
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 942
BstBI TTCGAA 1 cut(s) 295
BstC8I GCNNGC 4 cut(s) 565, 836, 1097, 1219
BstDEI CTNAG 4 cut(s) 363, 858, 960, 1119
BstEII GGTNACC 1 cut(s) 104
BstFNI CGCG 1 cut(s) 396
BstKTI GATC 9 cut(s) 42, 187, 463, 525, 535, 709, 826, 1003, 1339
BstMAI GTCTC 4 cut(s) 62, 347, 637, 745
BstMBI GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
BstMCI CGRYCG 2 cut(s) 46, 50
BstMWI GCNNNNNNNGC 7 cut(s) 584, 660, 710, 873, 910, 942, 1070
BstNSI RCATGY 3 cut(s) 37, 838, 1068
BstPI GGTNACC 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 634
BstSLI GKGCMC 1 cut(s) 320
BstUI CGCG 1 cut(s) 396
BstV1I GCAGC 4 cut(s) 596, 620, 674, 1304
BstV2I GAAGAC 2 cut(s) 173, 628
BstX2I RGATCY 4 cut(s) 706, 823, 1000, 1336
BstYI RGATCY 4 cut(s) 706, 823, 1000, 1336
BsuRI GGCC 6 cut(s) 53, 275, 369, 813, 876, 1092
BtsI GCAGTG 2 cut(s) 643, 1272
BtsIMutI CAGTG 7 cut(s) 241, 318, 587, 625, 643, 1261, 1272
BveI ACCTGC 1 cut(s) 872
Cac8I GCNNGC 4 cut(s) 565, 836, 1097, 1219
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 4 cut(s) 52, 367, 812, 1186
CseI GACGC 2 cut(s) 10, 385
CspAI ACCGGT 1 cut(s) 45
DdeI CTNAG 4 cut(s) 363, 858, 960, 1119
DpnI GATC 9 cut(s) 41, 186, 462, 524, 534, 708, 825, 1002, 1338
DpnII GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
DrdI GACNNNNNNGTC 1 cut(s) 1022
DseDI GACNNNNNNGTC 1 cut(s) 1022
EaeI YGGCCR 2 cut(s) 273, 1090
Eam1104I CTCTTC 3 cut(s) 2, 292, 921
EarI CTCTTC 3 cut(s) 2, 292, 921
Eco130I CCWWGG 2 cut(s) 819, 996
Eco31I GGTCTC 2 cut(s) 62, 637
Eco47I GGWCC 1 cut(s) 1186
Eco57I CTGAAG 2 cut(s) 276, 1260
Eco91I GGTNACC 1 cut(s) 104
EcoO65I GGTNACC 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 819, 996
EcoT22I ATGCAT 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 819, 996
FalI AAGNNNNNCTT 6 cut(s) 489, 521, 1122, 1154, 1187, 1219
FauI CCCGC 1 cut(s) 1348
Fnu4HI GCNGC 5 cut(s) 585, 634, 688, 1093, 1318
Fsp4HI GCNGC 5 cut(s) 585, 634, 688, 1093, 1318
FspBI CTAG 3 cut(s) 572, 651, 1053
GluI GCNGC 5 cut(s) 585, 634, 688, 1093, 1318
HaeIII GGCC 6 cut(s) 53, 275, 369, 813, 876, 1092
HapII CCGG 4 cut(s) 46, 354, 370, 1061
HgaI GACGC 2 cut(s) 10, 385
Hin1I GRCGYC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 3 cut(s) 672, 897, 1397
HinfI GANTC 7 cut(s) 239, 285, 323, 490, 1155, 1191, 1250
HpaI GTTAAC 1 cut(s) 697
HpaII CCGG 4 cut(s) 46, 354, 370, 1061
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 4 cut(s) 148, 318, 697, 1136
Hpy188I TCNGA 2 cut(s) 465, 951
Hpy188III TCNNGA 7 cut(s) 526, 668, 742, 1004, 1114, 1127, 1159
Hpy8I GTNNAC 4 cut(s) 148, 318, 697, 1136
Hpy99I CGWCG 2 cut(s) 26, 401
HpyAV CCTTC 6 cut(s) 66, 351, 523, 825, 1124, 1156
HpyCH4III ACNGT 2 cut(s) 629, 739
HpyF10VI GCNNNNNNNGC 7 cut(s) 584, 660, 710, 873, 910, 942, 1070
HpyF3I CTNAG 4 cut(s) 363, 858, 960, 1119
Hsp92I GRCGYC 1 cut(s) 21
KspAI GTTAAC 1 cut(s) 697
Kzo9I GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
LmnI GCTCC 4 cut(s) 221, 515, 572, 1165
Lsp1109I GCAGC 4 cut(s) 596, 620, 674, 1304
LweI GCATC 2 cut(s) 257, 268
MaeI CTAG 3 cut(s) 572, 651, 1053
MaeIII GTNAC 3 cut(s) 104, 250, 844
MalI GATC 9 cut(s) 41, 186, 462, 524, 534, 708, 825, 1002, 1338
MboI GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
MboII GAAGA 9 cut(s) 19, 178, 309, 633, 761, 938, 1002, 1346, 1405
MfeI CAATTG 1 cut(s) 1305
MflI RGATCY 4 cut(s) 706, 823, 1000, 1336
MhlI GDGCHC 2 cut(s) 33, 320
MluCI AATT 9 cut(s) 115, 193, 211, 230, 246, 373, 536, 1305, 1364
MluI ACGCGT 1 cut(s) 394
Mph1103I ATGCAT 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 768
MslI CAYNNNNRTG 1 cut(s) 780
MspI CCGG 4 cut(s) 46, 354, 370, 1061
MunI CAATTG 1 cut(s) 1305
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 7 cut(s) 584, 660, 710, 873, 910, 942, 1070
NdeII GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
NlaIV GGNNCC 3 cut(s) 54, 708, 1002
NmeAIII GCCGAG 1 cut(s) 902
NsiI ATGCAT 1 cut(s) 205
NspI RCATGY 3 cut(s) 37, 838, 1068
NspV TTCGAA 1 cut(s) 295
PaeI GCATGC 1 cut(s) 838
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 7 cut(s) 239, 285, 323, 490, 1155, 1191, 1250
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 5 cut(s) 586, 635, 689, 1094, 1319
PshBI ATTAAT 2 cut(s) 1245, 1388
PsiI TTATAA 1 cut(s) 224
PspEI GGTNACC 1 cut(s) 104
PspN4I GGNNCC 3 cut(s) 54, 708, 1002
PspPI GGNCC 4 cut(s) 52, 367, 812, 1186
PstI CTGCAG 1 cut(s) 638
PsuI RGATCY 4 cut(s) 706, 823, 1000, 1336
RseI CAYNNNNRTG 1 cut(s) 780
SatI GCNGC 5 cut(s) 585, 634, 688, 1093, 1318
Sau3AI GATC 9 cut(s) 39, 184, 460, 522, 532, 706, 823, 1000, 1336
Sau96I GGNCC 4 cut(s) 52, 367, 812, 1186
SduI GDGCHC 2 cut(s) 33, 320
SfaNI GCATC 2 cut(s) 257, 268
SfcI CTRYAG 1 cut(s) 634
SfuI TTCGAA 1 cut(s) 295
SinI GGWCC 1 cut(s) 1186
SmiMI CAYNNNNRTG 1 cut(s) 780
SmlI CTYRAG 3 cut(s) 742, 793, 1400
SmoI CTYRAG 3 cut(s) 742, 793, 1400
SpeI ACTAGT 1 cut(s) 1052
SphI GCATGC 1 cut(s) 838
Sse9I AATT 9 cut(s) 115, 193, 211, 230, 246, 373, 536, 1305, 1364
SsiI CCGC 4 cut(s) 704, 1093, 1294, 1341
SspMI CTAG 3 cut(s) 572, 651, 1053
StyI CCWWGG 2 cut(s) 819, 996
TaaI ACNGT 2 cut(s) 629, 739
TaqI TCGA 4 cut(s) 42, 295, 786, 1311
TaqII GACCGA 1 cut(s) 1203
TasI AATT 9 cut(s) 115, 193, 211, 230, 246, 373, 536, 1305, 1364
TauI GCSGC 1 cut(s) 1095
TfiI GAWTC 7 cut(s) 239, 285, 323, 490, 1155, 1191, 1250
TscAI CASTG 7 cut(s) 241, 325, 594, 632, 643, 1261, 1272
TseI GCWGC 4 cut(s) 584, 633, 687, 1317
TspDTI ATGAA 9 cut(s) 108, 179, 301, 930, 1119, 1263, 1312, 1347, 1406
TspRI CASTG 7 cut(s) 241, 325, 594, 632, 643, 1261, 1272
VneI GTGCAC 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 1186
VspI ATTAAT 2 cut(s) 1245, 1388
XapI RAATTY 2 cut(s) 230, 373
XceI RCATGY 3 cut(s) 37, 838, 1068
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 3 cut(s) 572, 651, 1053
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.