Rroxscaffold_5G00361300

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003066
Physical Location & Seq
Forward (+)
41972649 .. 41979873
7225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00361300.1

Sequence Viewer

Length: 582 bp
ATGAGAGATAGAGGTGAAGCAGGTCCCTTGATGTTATTTATTGTAAAGTCTGGTAGCTTTGATAGACCATCACAAGTGGCCATTGCAAACGGAGCATCAACTAGTCAGTATAAGCCACCCAAGATCATAAGAAATCGTCAAATTGTTCACTTGCTTGAAAGGGTTCACGAACAATATGGACAGAGCATTGAATACAGCATACAGAATGAAGGACCCCAAAATCATATACTAGGAGTCCTGGTTCATGCTAAAGAGTCATTGAGTTCTGAGAAGAAAGAGAGAAACGGGTATGGAACTGGTGTTGGAAGCAGTGATGGGATAAAGAGCTTTGACTTTAAAATTCCGGAGGAGAGGATCCATGGAGGACCTCTCATGTTACTGTTTGGTGGTAATCTTGAGATATCGGCGTTGACCTCCAATCGGACTTGGATCTGGTTTCCATTGCTGCGGCGGCTACAGGCCGAGATAGGTTGCAAGGCGGCCACGGGGAGGTGTGCAAGGTTGAGGCGAGGCTACCCCAAGAATTTCAAGTCCAAAGGTGGCTGTAGGAGGAAGAAATGGCTTGGAGAAGAAAATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
DUF295 GH3

Protein Analysis

193

Amino Acids

21.58

Weight (kDa)

9.97

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3_C PF23572 2 - 47 5.2e-07 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 3 cut(s) 448, 451, 479
AclWI GGATC 3 cut(s) 349, 362, 437
AcoI YGGCCR 2 cut(s) 78, 480
AcsI RAATTY 2 cut(s) 339, 523
AfiI CCNNNNNNNGG 2 cut(s) 420, 489
AgsI TTSAA 3 cut(s) 158, 191, 529
AhlI ACTAGT 1 cut(s) 101
AjnI CCWGG 1 cut(s) 237
AloI GAACNNNNNNTCC 2 cut(s) 225, 257
AluBI AGCT 2 cut(s) 57, 327
AluI AGCT 2 cut(s) 57, 327
AlwI GGATC 3 cut(s) 349, 362, 437
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 3 cut(s) 78, 459, 480
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 2 cut(s) 339, 523
Asp700I GAANNNNTTC 1 cut(s) 162
AspS9I GGNCC 3 cut(s) 23, 212, 365
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 3 cut(s) 23, 212, 365
BalI TGGCCA 1 cut(s) 80
BamHI GGATCC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 432
BccI CCATC 2 cut(s) 76, 308
BciT130I CCWGG 1 cut(s) 239
BcuI ACTAGT 1 cut(s) 101
BfaI CTAG 2 cut(s) 102, 230
BfmI CTRYAG 2 cut(s) 455, 544
BfuAI ACCTGC 1 cut(s) 11
BisI GCNGC 4 cut(s) 446, 449, 452, 480
BlsI GCNGC 4 cut(s) 447, 450, 453, 481
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 3 cut(s) 23, 212, 365
BmgT120I GGNCC 3 cut(s) 23, 212, 365
BmiI GGNNCC 3 cut(s) 25, 214, 356
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 354, 386
BpuEI CTTGAG 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 358, 483
BsaWI WCCGGW 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 2 cut(s) 420, 489
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 2 cut(s) 81, 440
BseAI TCCGGA 1 cut(s) 343
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 358, 483
BseLI CCNNNNNNNGG 2 cut(s) 420, 489
BseMI GCAATG 2 cut(s) 81, 440
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 1 cut(s) 432
BshFI GGCC 3 cut(s) 80, 461, 482
BsiSI CCGG 1 cut(s) 344
BslFI GGGAC 1 cut(s) 9
BslI CCNNNNNNNGG 2 cut(s) 420, 489
BsmFI GGGAC 1 cut(s) 9
BsnI GGCC 3 cut(s) 80, 461, 482
Bsp13I TCCGGA 1 cut(s) 343
Bsp143I GATC 3 cut(s) 123, 354, 429
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 3 cut(s) 448, 451, 479
BspANI GGCC 3 cut(s) 80, 461, 482
BspCNI CTCAG 1 cut(s) 259
BspEI TCCGGA 1 cut(s) 343
BspLI GGNNCC 3 cut(s) 25, 214, 356
BspMI ACCTGC 1 cut(s) 11
BspPI GGATC 3 cut(s) 349, 362, 437
BsrDI GCAATG 2 cut(s) 81, 440
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 2 cut(s) 358, 483
BssMI GATC 3 cut(s) 123, 354, 429
BssT1I CCWWGG 1 cut(s) 358
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 381
BstDEI CTNAG 1 cut(s) 267
BstDSI CCRYGG 2 cut(s) 358, 483
BstKTI GATC 3 cut(s) 126, 357, 432
BstMBI GATC 3 cut(s) 123, 354, 429
BstMWI GCNNNNNNNGC 2 cut(s) 92, 451
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 2 cut(s) 455, 544
BstV1I GCAGC 1 cut(s) 432
BstX2I RGATCY 2 cut(s) 354, 429
BstYI RGATCY 2 cut(s) 354, 429
BsuRI GGCC 3 cut(s) 80, 461, 482
BtgI CCRYGG 2 cut(s) 358, 483
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 316
BveI ACCTGC 1 cut(s) 11
Cfr13I GGNCC 3 cut(s) 23, 212, 365
CviAII CATG 3 cut(s) 245, 359, 373
DdeI CTNAG 1 cut(s) 267
DpnI GATC 3 cut(s) 125, 356, 431
DpnII GATC 3 cut(s) 123, 354, 429
DraI TTTAAA 1 cut(s) 337
EaeI YGGCCR 2 cut(s) 78, 480
Eco130I CCWWGG 1 cut(s) 358
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 3 cut(s) 23, 212, 365
EcoO109I RGGNCCY 3 cut(s) 23, 212, 365
EcoRII CCWGG 1 cut(s) 237
EcoRV GATATC 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 358
FaeI CATG 3 cut(s) 248, 362, 376
FaqI GGGAC 1 cut(s) 9
FatI CATG 3 cut(s) 244, 358, 372
Fnu4HI GCNGC 4 cut(s) 446, 449, 452, 480
Fsp4HI GCNGC 4 cut(s) 446, 449, 452, 480
FspBI CTAG 2 cut(s) 102, 230
GluI GCNGC 4 cut(s) 446, 449, 452, 480
HaeIII GGCC 3 cut(s) 80, 461, 482
HapII CCGG 1 cut(s) 344
Hin1II CATG 3 cut(s) 248, 362, 376
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HinfI GANTC 2 cut(s) 234, 254
HpaII CCGG 1 cut(s) 344
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 3 cut(s) 148, 166, 411
Hpy188I TCNGA 2 cut(s) 268, 423
Hpy188III TCNNGA 3 cut(s) 167, 344, 395
Hpy8I GTNNAC 3 cut(s) 148, 166, 411
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4V TGCA 3 cut(s) 86, 474, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 451
HpyF3I CTNAG 1 cut(s) 267
Hsp92II CATG 3 cut(s) 248, 362, 376
Kpn2I TCCGGA 1 cut(s) 343
Kzo9I GATC 3 cut(s) 123, 354, 429
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 8 cut(s) 6, 36, 224, 251, 282, 357, 418, 443
Lsp1109I GCAGC 1 cut(s) 432
LweI GCATC 1 cut(s) 104
MaeI CTAG 2 cut(s) 102, 230
MaeIII GTNAC 1 cut(s) 375
MalI GATC 3 cut(s) 125, 356, 431
MboI GATC 3 cut(s) 123, 354, 429
MboII GAAGA 3 cut(s) 283, 565, 581
MflI RGATCY 2 cut(s) 354, 429
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 3 cut(s) 141, 339, 523
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 2 cut(s) 243, 263
MmeI TCCRAC 1 cut(s) 283
Mox20I TGGCCA 1 cut(s) 80
MroI TCCGGA 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 162
MscI TGGCCA 1 cut(s) 80
MseI TTAA 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 80
MspI CCGG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 92, 451
NcoI CCATGG 1 cut(s) 358
NdeII GATC 3 cut(s) 123, 354, 429
NlaIII CATG 3 cut(s) 248, 362, 376
NlaIV GGNNCC 3 cut(s) 25, 214, 356
NmeAIII GCCGAG 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 162
PkrI GCNGC 4 cut(s) 447, 450, 453, 481
PleI GAGTC 2 cut(s) 242, 262
PpsI GAGTC 2 cut(s) 242, 262
PpuMI RGGWCCY 3 cut(s) 23, 212, 365
Psp5II RGGWCCY 3 cut(s) 23, 212, 365
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 25, 214, 356
PspPI GGNCC 3 cut(s) 23, 212, 365
PspPPI RGGWCCY 3 cut(s) 23, 212, 365
PsuI RGATCY 2 cut(s) 354, 429
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 4 cut(s) 446, 449, 452, 480
Sau3AI GATC 3 cut(s) 123, 354, 429
Sau96I GGNCC 3 cut(s) 23, 212, 365
SchI GAGTC 2 cut(s) 243, 263
ScrFI CCNGG 1 cut(s) 239
SfaNI GCATC 1 cut(s) 104
SfcI CTRYAG 2 cut(s) 455, 544
SinI GGWCC 3 cut(s) 23, 212, 365
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
SpeI ACTAGT 1 cut(s) 101
Sse9I AATT 3 cut(s) 141, 339, 523
SsiI CCGC 3 cut(s) 448, 451, 479
SspMI CTAG 2 cut(s) 102, 230
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 1 cut(s) 381
TasI AATT 3 cut(s) 141, 339, 523
TauI GCSGC 3 cut(s) 451, 454, 482
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 316
TseI GCWGC 1 cut(s) 445
TspDTI ATGAA 2 cut(s) 222, 233
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 1 cut(s) 316
VpaK11BI GGWCC 3 cut(s) 23, 212, 365
XapI RAATTY 2 cut(s) 339, 523
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 2 cut(s) 102, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.