Rw7G020230

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003066
Physical Location & Seq
Forward (+)
22834924 .. 22838188
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020230.1

Sequence Viewer

Length: 666 bp
ATGACAGTAGACAGCGCCACCATGGAAGACATGGAAGCCAAGGCCGAGCAGCTCGGTATCCAATTCTCCTCCATAGACTTGAACTCTGCACACCCACCTCACTTTAATTTCAGTGAATGCCAAGAAGTGTTTTACGACGACAACGGTTTCATCCCCAATATTGAGTTTGGGAGTGTTATCGTGGTTAATAACCTTCCCATTGTGGAGGCAGAGATGGCCCAGGAGCTCGAAAGCGAGATTCGGGTGGTGTTTAGTGAAGTTGGTGTTATTAAAGAAGATGGGTTTTCGATGCCTGTGAATCCCAGCACCCAAGAAACCTTGGGTCATTGTTTCATAGAGTTTAATTCTCGTCAGGAAGCCGTACTTGCTAGGAGCAAGAGAGATGGATACAAGTTTGGTAACTGCAAGTTGTCAGTAAGCCTTTATGATGCCTTTGGCAGTGTAGTTGCTACCCCTGACATGGGGACTTATTTAGAGACTAATGCATCCTTTGGTGGGTCCTCAAGGTATCCAGGGTCACCTTCCTTGCTTAGTCCTGTAACAGAGGAAGACGAAGAACCCGTTCATAGTGATGAAACTAGTGCAGATGACCACGATGACATAATGCAGTGGAGTGAGCGAGACAATTATATGAGCAGTGAGGAATTTAGGGAAGTCATTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

221

Amino Acids

24.61

Weight (kDa)

4.14

Isoelectric Point (pI)

59.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 77 - 137 9.5e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AcsI RAATTY 1 cut(s) 644
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 3 cut(s) 460, 461, 495
AgsI TTSAA 2 cut(s) 82, 662
AhlI ACTAGT 1 cut(s) 578
AjnI CCWGG 2 cut(s) 219, 511
AluBI AGCT 2 cut(s) 52, 226
AluI AGCT 2 cut(s) 52, 226
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 470, 615
AoxI GGCC 2 cut(s) 42, 216
ApeKI GCWGC 1 cut(s) 49
ApoI RAATTY 1 cut(s) 644
Asp700I GAANNNNTTC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 2 cut(s) 217, 498
AsuHPI GGTGA 1 cut(s) 510
AvaII GGWCC 1 cut(s) 498
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 2 cut(s) 33, 555
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 61
BccI CCATC 3 cut(s) 208, 272, 377
BceAI ACGGC 1 cut(s) 344
BciT130I CCWGG 2 cut(s) 221, 513
BciVI GTATCC 3 cut(s) 68, 380, 519
BcoDI GTCTC 2 cut(s) 470, 615
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 2 cut(s) 369, 579
BfoI RGCGCY 1 cut(s) 18
BfuI GTATCC 3 cut(s) 68, 380, 519
BisI GCNGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 51
Bme1390I CCNGG 2 cut(s) 221, 513
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 217, 498
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 2 cut(s) 221, 513
BmsI GCATC 3 cut(s) 279, 418, 494
BpiI GAAGAC 2 cut(s) 33, 555
BpuEI CTTGAG 1 cut(s) 487
BsaJI CCNNGG 5 cut(s) 21, 39, 219, 318, 512
Bsc4I CCNNNNNNNGG 3 cut(s) 460, 461, 495
BseBI CCWGG 2 cut(s) 221, 513
BseDI CCNNGG 5 cut(s) 21, 39, 219, 318, 512
BseGI GGATG 2 cut(s) 150, 485
BseLI CCNNNNNNNGG 3 cut(s) 460, 461, 495
BseRI GAGGAG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 61
BseYI CCCAGC 1 cut(s) 302
BsgI GTGCAG 2 cut(s) 72, 603
BshFI GGCC 2 cut(s) 44, 218
BsiHKAI GWGCWC 1 cut(s) 228
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 3 cut(s) 460, 461, 495
BsmAI GTCTC 2 cut(s) 470, 615
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 2 cut(s) 44, 218
Bsp1286I GDGCHC 1 cut(s) 228
Bsp19I CCATGG 1 cut(s) 21
BspANI GGCC 2 cut(s) 44, 218
BspLI GGNNCC 1 cut(s) 499
BssECI CCNNGG 5 cut(s) 21, 39, 219, 318, 512
BssT1I CCWWGG 3 cut(s) 21, 39, 318
Bst2UI CCWGG 2 cut(s) 221, 513
Bst4CI ACNGT 2 cut(s) 7, 146
BstDEI CTNAG 1 cut(s) 530
BstDSI CCRYGG 1 cut(s) 21
BstEII GGTNACC 1 cut(s) 516
BstF5I GGATG 2 cut(s) 150, 485
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstMAI GTCTC 2 cut(s) 470, 615
BstMWI GCNNNNNNNGC 2 cut(s) 215, 365
BstNI CCWGG 2 cut(s) 221, 513
BstPI GGTNACC 1 cut(s) 516
BstSCI CCNGG 2 cut(s) 219, 511
BstV1I GCAGC 1 cut(s) 61
BstV2I GAAGAC 2 cut(s) 33, 555
BsuI GTATCC 3 cut(s) 68, 380, 519
BsuRI GGCC 2 cut(s) 44, 218
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 2 cut(s) 150, 485
BtsI GCAGTG 3 cut(s) 445, 614, 643
BtsIMutI CAGTG 4 cut(s) 118, 445, 614, 643
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 217, 498
Csp6I GTAC 1 cut(s) 362
CviAII CATG 3 cut(s) 22, 31, 460
CviJI RGCY 7 cut(s) 38, 44, 52, 218, 226, 359, 420
CviKI_1 RGCY 7 cut(s) 38, 44, 52, 218, 226, 359, 420
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 1 cut(s) 530
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 3 cut(s) 21, 39, 318
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 226
Eco91I GGTNACC 1 cut(s) 516
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 498
EcoO65I GGTNACC 1 cut(s) 516
EcoRII CCWGG 2 cut(s) 219, 511
EcoT14I CCWWGG 3 cut(s) 21, 39, 318
EcoT22I ATGCAT 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 3 cut(s) 21, 39, 318
FaeI CATG 3 cut(s) 25, 34, 463
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 478
FatI CATG 3 cut(s) 21, 30, 459
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 1 cut(s) 50
FokI GGATG 2 cut(s) 137, 472
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 50
FspBI CTAG 2 cut(s) 369, 579
GlaI GCGC 1 cut(s) 16
GluI GCNGC 1 cut(s) 50
GsaI CCCAGC 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 2 cut(s) 44, 218
HhaI GCGC 1 cut(s) 17
Hin1II CATG 3 cut(s) 25, 34, 463
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HinfI GANTC 2 cut(s) 238, 298
HphI GGTGA 1 cut(s) 510
Hpy166II GTNNAC 1 cut(s) 10
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 203, 531
HpyCH4III ACNGT 2 cut(s) 7, 146
HpyCH4V TGCA 5 cut(s) 89, 405, 485, 584, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 365
HpyF3I CTNAG 1 cut(s) 530
Hsp92II CATG 3 cut(s) 25, 34, 463
HspAI GCGC 1 cut(s) 15
LmnI GCTCC 2 cut(s) 223, 372
LpnPI CCDG 9 cut(s) 206, 233, 306, 316, 338, 468, 498, 525, 549
Lsp1109I GCAGC 1 cut(s) 61
LweI GCATC 3 cut(s) 279, 418, 494
MaeI CTAG 2 cut(s) 369, 579
MaeIII GTNAC 3 cut(s) 398, 516, 538
MboII GAAGA 4 cut(s) 38, 287, 560, 566
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 5 cut(s) 62, 106, 343, 625, 644
MnlI CCTC 6 cut(s) 79, 108, 199, 511, 538, 634
Mph1103I ATGCAT 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 4 cut(s) 105, 186, 270, 342
MslI CAYNNNNRTG 1 cut(s) 570
MspR9I CCNGG 2 cut(s) 221, 513
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 2 cut(s) 221, 513
MwoI GCNNNNNNNGC 2 cut(s) 215, 365
NcoI CCATGG 1 cut(s) 21
NlaIII CATG 3 cut(s) 25, 34, 463
NlaIV GGNNCC 1 cut(s) 499
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 516
NsiI ATGCAT 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PctI GAATGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 2 cut(s) 238, 298
PkrI GCNGC 1 cut(s) 51
PpuMI RGGWCCY 1 cut(s) 498
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 219, 511
PspEI GGTNACC 1 cut(s) 516
PspFI CCCAGC 1 cut(s) 302
PspGI CCWGG 2 cut(s) 219, 511
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 2 cut(s) 217, 498
PspPPI RGGWCCY 1 cut(s) 498
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 570
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 4 cut(s) 105, 186, 270, 342
SatI GCNGC 1 cut(s) 50
Sau96I GGNCC 2 cut(s) 217, 498
ScrFI CCNGG 2 cut(s) 221, 513
SduI GDGCHC 1 cut(s) 228
SetI ASST 7 cut(s) 54, 100, 195, 228, 320, 509, 523
SfaNI GCATC 3 cut(s) 279, 418, 494
SinI GGWCC 1 cut(s) 498
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
SpeI ACTAGT 1 cut(s) 578
Sse9I AATT 5 cut(s) 62, 106, 343, 625, 644
SspI AATATT 1 cut(s) 160
SspMI CTAG 2 cut(s) 369, 579
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 219, 511
StyI CCWWGG 3 cut(s) 21, 39, 318
TaaI ACNGT 2 cut(s) 7, 146
TaqI TCGA 2 cut(s) 228, 287
TasI AATT 5 cut(s) 62, 106, 343, 625, 644
TfiI GAWTC 2 cut(s) 238, 298
Tru1I TTAA 4 cut(s) 105, 186, 270, 342
Tru9I TTAA 4 cut(s) 105, 186, 270, 342
TscAI CASTG 4 cut(s) 118, 445, 614, 643
TseFI GTSAC 1 cut(s) 516
TseI GCWGC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 516
TspDTI ATGAA 4 cut(s) 139, 322, 554, 588
TspRI CASTG 4 cut(s) 118, 445, 614, 643
VpaK11BI GGWCC 1 cut(s) 498
XapI RAATTY 1 cut(s) 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 2 cut(s) 369, 579
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.