MD06G1040600.v1.1

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003066
Physical Location & Seq
Reverse (-)
5203073 .. 5204630
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1040600.v1.1.491

Sequence Viewer

Length: 684 bp
ATGACAATAGACACCGCCACCATGGGAGAGCAAGAGCAGCTGCGCCTTCAACTCTCCGCCCTCCACTTAAACACTCCGCCTCCTCCGCCGACTCCTTACCAAAACTTGTATGAGTCTGATGACCAACAAGTCTTCTACAACGACGACGGCTTGCTGTTCGACATCGGGCTCGGAAACGTGATTGTCGTCGATAACCTTCCAATTGTTGATGCCAGGATGGCCGAGAAGCTCGAAAGCGAAATCCGACGAGCGTTTAGTGAAGTTGGTGTGATTAAACACGATGGGGTTTCCGTGCCTTTGAATCCCCAAACTCACGAAAGCTTGGGTCGTTGTTTTATAGAGTTTAATTCTCGTCAGGAAGCTGAAGTTGCTAGGGAGAAGAGAGATGGATACAACTTCCATACGCATACACTATCAGTAAGCCTGTATGATGATTTGGACAGGTTCGTTGATATCCTTGATGTGGGGACTACCATTGAAACTTCTCCAGCCGCTTTTATGTCCTCAAGGCATACGGGGCCGCCCCCCTTGCTTAGTCCCTTGAAAGAGGAAGATGAAGAAGTACTCGAAGAAGCACAGCGAGTAAACCACGGCAGAAAAGTAAGAGACGAAAAGGAGCAAGATTTCGGGGAAGTCACGGCATCGATGGGAATGATGAGTATTAGAGGGGAAAGCATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

228

Amino Acids

25.55

Weight (kDa)

4.56

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 78 - 138 7e-08 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AciI CCGC 6 cut(s) 15, 57, 77, 86, 492, 521
AcoI YGGCCR 1 cut(s) 219
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 1 cut(s) 564
AfiI CCNNNNNNNGG 1 cut(s) 463
AgsI TTSAA 4 cut(s) 50, 301, 479, 544
AjnI CCWGG 1 cut(s) 212
AluBI AGCT 4 cut(s) 40, 229, 321, 362
AluI AGCT 4 cut(s) 40, 229, 321, 362
Alw26I GTCTC 1 cut(s) 600
AoxI GGCC 2 cut(s) 219, 518
ApeKI GCWGC 2 cut(s) 37, 40
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 518
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 27, 49
BccI CCATC 4 cut(s) 211, 275, 380, 640
BceAI ACGGC 3 cut(s) 163, 607, 654
BciT130I CCWGG 1 cut(s) 214
BciVI GTATCC 1 cut(s) 383
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 1 cut(s) 372
BfuI GTATCC 1 cut(s) 383
BglI GCCNNNNNGGC 1 cut(s) 218
BisI GCNGC 4 cut(s) 38, 41, 492, 521
BlsI GCNGC 4 cut(s) 39, 42, 493, 522
BmcAI AGTACT 1 cut(s) 564
Bme1390I CCNGG 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 1 cut(s) 519
BmrFI CCNGG 1 cut(s) 214
BmsI GCATC 2 cut(s) 199, 650
BpiI GAAGAC 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 490
Bsa29I ATCGAT 1 cut(s) 644
BsaJI CCNNGG 2 cut(s) 21, 589
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 1 cut(s) 463
BseBI CCWGG 1 cut(s) 214
BseCI ATCGAT 1 cut(s) 644
BseDI CCNNGG 2 cut(s) 21, 589
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 463
BseRI GAGGAG 1 cut(s) 72
BseXI GCAGC 2 cut(s) 27, 49
BshFI GGCC 2 cut(s) 221, 520
BshVI ATCGAT 1 cut(s) 644
BslFI GGGAC 2 cut(s) 481, 522
BslI CCNNNNNNNGG 1 cut(s) 463
BsmAI GTCTC 1 cut(s) 600
BsmBI CGTCTC 1 cut(s) 600
BsmFI GGGAC 2 cut(s) 481, 522
BsnI GGCC 2 cut(s) 221, 520
Bsp1286I GDGCHC 1 cut(s) 171
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 6 cut(s) 15, 57, 77, 86, 492, 521
BspANI GGCC 2 cut(s) 221, 520
BspDI ATCGAT 1 cut(s) 644
BspLI GGNNCC 1 cut(s) 519
BssECI CCNNGG 2 cut(s) 21, 589
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 1 cut(s) 533
BstDSI CCRYGG 2 cut(s) 21, 589
BstF5I GGATG 1 cut(s) 222
BstHHI GCGC 1 cut(s) 45
BstMAI GTCTC 1 cut(s) 600
BstMWI GCNNNNNNNGC 6 cut(s) 37, 85, 218, 368, 517, 529
BstNI CCWGG 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 27, 49
BstV2I GAAGAC 1 cut(s) 124
Bsu15I ATCGAT 1 cut(s) 644
BsuI GTATCC 1 cut(s) 383
BsuRI GGCC 2 cut(s) 221, 520
BsuTUI ATCGAT 1 cut(s) 644
BtgI CCRYGG 2 cut(s) 21, 589
BtsCI GGATG 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 152
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 518
ClaI ATCGAT 1 cut(s) 644
Csp6I GTAC 1 cut(s) 563
CviAII CATG 2 cut(s) 22, 676
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 1 cut(s) 533
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
EciI GGCGGA 3 cut(s) 46, 66, 75
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 171
Eco32I GATATC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 212
EcoRV GATATC 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 600
FaeI CATG 2 cut(s) 25, 679
FaiI YATR 9 cut(s) 23, 111, 338, 402, 408, 429, 500, 513, 677
FaqI GGGAC 2 cut(s) 481, 522
FatI CATG 2 cut(s) 21, 675
Fnu4HI GCNGC 4 cut(s) 38, 41, 492, 521
FokI GGATG 1 cut(s) 229
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 4 cut(s) 38, 41, 492, 521
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 44
GluI GCNGC 4 cut(s) 38, 41, 492, 521
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 2 cut(s) 221, 520
HhaI GCGC 1 cut(s) 45
Hin1II CATG 2 cut(s) 25, 679
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 3 cut(s) 91, 113, 301
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 3 cut(s) 118, 173, 245
Hpy188III TCNNGA 2 cut(s) 314, 356
Hpy8I GTNNAC 1 cut(s) 586
Hpy99I CGWCG 4 cut(s) 146, 149, 191, 249
HpyAV CCTTC 2 cut(s) 56, 206
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 6 cut(s) 37, 85, 218, 368, 517, 529
HpyF3I CTNAG 1 cut(s) 533
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 2 cut(s) 25, 679
HspAI GCGC 1 cut(s) 43
LmnI GCTCC 1 cut(s) 616
LpnPI CCDG 6 cut(s) 199, 226, 341, 427, 437, 501
Lsp1109I GCAGC 2 cut(s) 27, 49
LweI GCATC 2 cut(s) 199, 650
MaeI CTAG 1 cut(s) 372
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 634
MboII GAAGA 5 cut(s) 124, 391, 563, 569, 581
MfeI CAATTG 1 cut(s) 201
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 2 cut(s) 201, 346
MlyI GAGTC 2 cut(s) 85, 122
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 6 cut(s) 71, 90, 93, 514, 541, 659
MseI TTAA 3 cut(s) 68, 273, 345
MspA1I CMGCKG 1 cut(s) 40
MspR9I CCNGG 1 cut(s) 214
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 214
MwoI GCNNNNNNNGC 6 cut(s) 37, 85, 218, 368, 517, 529
NcoI CCATGG 1 cut(s) 21
NlaIII CATG 2 cut(s) 25, 679
NlaIV GGNNCC 1 cut(s) 519
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PfeI GAWTC 1 cut(s) 301
PkrI GCNGC 4 cut(s) 39, 42, 493, 522
PleI GAGTC 2 cut(s) 85, 121
PpsI GAGTC 2 cut(s) 85, 121
Psp6I CCWGG 1 cut(s) 212
PspGI CCWGG 1 cut(s) 212
PspN4I GGNNCC 1 cut(s) 519
PspPI GGNCC 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
SaqAI TTAA 3 cut(s) 68, 273, 345
SatI GCNGC 4 cut(s) 38, 41, 492, 521
Sau96I GGNCC 1 cut(s) 518
ScaI AGTACT 1 cut(s) 564
SchI GAGTC 2 cut(s) 85, 122
ScrFI CCNGG 1 cut(s) 214
SduI GDGCHC 1 cut(s) 171
SetI ASST 7 cut(s) 42, 180, 198, 231, 323, 364, 446
SfaNI GCATC 2 cut(s) 199, 650
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 2 cut(s) 201, 346
SsiI CCGC 6 cut(s) 15, 57, 77, 86, 492, 521
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 21
TaiI ACGT 1 cut(s) 180
TaqI TCGA 5 cut(s) 159, 189, 231, 567, 644
TasI AATT 2 cut(s) 201, 346
TatI WGTACW 1 cut(s) 562
TauI GCSGC 2 cut(s) 494, 523
TfiI GAWTC 1 cut(s) 301
Tru1I TTAA 3 cut(s) 68, 273, 345
Tru9I TTAA 3 cut(s) 68, 273, 345
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 2 cut(s) 37, 40
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 1 cut(s) 570
TspGWI ACGGA 1 cut(s) 280
XspI CTAG 1 cut(s) 372
ZrmI AGTACT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.