Prupe.5G046800_v2.0.a1

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0003066
Physical Location & Seq
Reverse (-)
5005007 .. 5012863
7857 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G046800.1

Sequence Viewer

Length: 684 bp
ATGGAAGATATCCAGGCCAAGGCCGAGGAGCTCCGTCTCCAACTCTCCTCCTTCCACTTCAACTCTGCAGCTCCACCTCCTAACCAAGACTTATATAATAATAATACTGAGAACAATTTTGGTGATGGCCAAGAAGTGTTCTACGACGACGACGGTTTGCTTTTCAATATTGGGTTCGGAAATGTGATCGTTGTTGATAACCTTCCGATTGTGAAGGCGGAGATGGCCCAGCAGCTCGAAAGCGAAATCCGACGAGTGTTTAGTGAAGTTGGTGTTATTAAAGAAGATGGGTTTTCGATGCCTCTTAATCCCCTGAACCAGAAAACCTTGGGTCGTTGTTTTATAGAGTTCAGTTCTCGTCAGGAAGCTGAACTTGCTAGGGTCAAGAGAGATGGATACAATTTCAATACACAAGCGCTGTCAGTAACCCTGTATGATGACTTGGACAGGTTCATTGATATCACTGATGTGGGGACAACACCAGAAACTAATCCATCCGCTTGTTTGGTAATTTTCCTAACCTCTCTTTGCCTATCAACTATCGTTTTATTGATTTTATATTGTTCTTATGCTTGTAGTCTTGTCTATCAACTCCACCTTTCTATATTCAAAACCGAAGTCTGCCTACTTCTATGGCACATGGCACATTTAGATATTGTATACCTTCATATGGATAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

228

Amino Acids

25.75

Weight (kDa)

4.51

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 660
AciI CCGC 2 cut(s) 218, 498
AcoI YGGCCR 1 cut(s) 127
AfeI AGCGCT 1 cut(s) 417
AfiI CCNNNNNNNGG 2 cut(s) 19, 670
AgsI TTSAA 4 cut(s) 61, 166, 406, 610
AjnI CCWGG 1 cut(s) 12
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 4 cut(s) 31, 71, 235, 368
AluI AGCT 4 cut(s) 31, 71, 235, 368
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 41
Aor51HI AGCGCT 1 cut(s) 417
AoxI GGCC 4 cut(s) 15, 21, 127, 225
ApeKI GCWGC 2 cut(s) 68, 232
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AspLEI GCGC 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 134
BalI TGGCCA 1 cut(s) 129
BanII GRGCYC 1 cut(s) 33
BarI GAAGNNNNNNTAC 2 cut(s) 609, 641
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 80, 244
BccI CCATC 5 cut(s) 119, 217, 281, 386, 502
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 419
BfuI GTATCC 1 cut(s) 389
BisI GCNGC 2 cut(s) 69, 233
BlsI GCNGC 2 cut(s) 70, 234
Bme1390I CCNGG 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 226
BmrFI CCNGG 1 cut(s) 14
BmsI GCATC 1 cut(s) 288
BsaJI CCNNGG 3 cut(s) 18, 24, 327
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 670
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 18, 24, 327
BseGI GGATG 1 cut(s) 494
BseLI CCNNNNNNNGG 2 cut(s) 19, 670
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 2 cut(s) 37, 41
BseXI GCAGC 2 cut(s) 80, 244
BseYI CCCAGC 1 cut(s) 228
BshFI GGCC 4 cut(s) 17, 23, 129, 227
BsiHKAI GWGCWC 1 cut(s) 33
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 2 cut(s) 19, 670
BsmAI GTCTC 1 cut(s) 41
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 4 cut(s) 17, 23, 129, 227
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 1 cut(s) 186
BspACI CCGC 2 cut(s) 218, 498
BspANI GGCC 4 cut(s) 17, 23, 129, 227
BspCNI CTCAG 1 cut(s) 100
BspMAI CTGCAG 1 cut(s) 70
BssECI CCNNGG 3 cut(s) 18, 24, 327
BssMI GATC 1 cut(s) 186
BssNAI GTATAC 1 cut(s) 661
BssT1I CCWWGG 2 cut(s) 18, 327
Bst1107I GTATAC 1 cut(s) 661
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 494
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 1 cut(s) 189
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 1 cut(s) 186
BstMWI GCNNNNNNNGC 2 cut(s) 224, 374
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 12
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 2 cut(s) 80, 244
BstZ17I GTATAC 1 cut(s) 661
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 4 cut(s) 17, 23, 129, 227
BtsCI GGATG 1 cut(s) 494
BtsIMutI CAGTG 1 cut(s) 462
CfoI GCGC 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 226
CviAII CATG 1 cut(s) 640
CviJI RGCY 8 cut(s) 17, 23, 31, 71, 129, 227, 235, 368
CviKI_1 RGCY 8 cut(s) 17, 23, 31, 71, 129, 227, 235, 368
DdeI CTNAG 1 cut(s) 108
DpnI GATC 1 cut(s) 188
DpnII GATC 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 127
EciI GGCGGA 1 cut(s) 233
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 2 cut(s) 18, 327
Eco24I GRGCYC 1 cut(s) 33
Eco32I GATATC 2 cut(s) 10, 460
Eco47III AGCGCT 1 cut(s) 417
Eco53kI GAGCTC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 12
EcoRV GATATC 2 cut(s) 10, 460
EcoT14I CCWWGG 2 cut(s) 18, 327
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 18, 327
Esp3I CGTCTC 1 cut(s) 41
FaeI CATG 1 cut(s) 643
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 487
FatI CATG 1 cut(s) 639
FauNDI CATATG 1 cut(s) 669
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 2 cut(s) 69, 233
FokI GGATG 1 cut(s) 481
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 2 cut(s) 69, 233
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 417
GluI GCNGC 2 cut(s) 69, 233
GsaI CCCAGC 1 cut(s) 232
HaeII RGCGCY 1 cut(s) 419
HaeIII GGCC 4 cut(s) 17, 23, 129, 227
HhaI GCGC 1 cut(s) 418
Hin1II CATG 1 cut(s) 643
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 3 cut(s) 179, 207, 251
Hpy188III TCNNGA 2 cut(s) 362, 385
Hpy8I GTNNAC 1 cut(s) 661
Hpy99I CGWCG 4 cut(s) 149, 152, 155, 255
HpyAV CCTTC 4 cut(s) 61, 208, 212, 674
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 1 cut(s) 68
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 374
HpyF3I CTNAG 1 cut(s) 108
Hsp92II CATG 1 cut(s) 643
HspAI GCGC 1 cut(s) 416
Kzo9I GATC 1 cut(s) 186
LmnI GCTCC 3 cut(s) 28, 36, 76
LpnPI CCDG 8 cut(s) 26, 242, 326, 332, 347, 433, 443, 495
Lsp1109I GCAGC 2 cut(s) 80, 244
LweI GCATC 1 cut(s) 288
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 424
MalI GATC 1 cut(s) 188
MboI GATC 1 cut(s) 186
MboII GAAGA 2 cut(s) 17, 296
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 4 cut(s) 115, 400, 510, 677
MluNI TGGCCA 1 cut(s) 129
MmeI TCCRAC 2 cut(s) 64, 274
MnlI CCTC 5 cut(s) 19, 58, 87, 312, 532
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 2 cut(s) 279, 306
MslI CAYNNNNRTG 1 cut(s) 467
Msp20I TGGCCA 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 2 cut(s) 224, 374
NdeI CATATG 1 cut(s) 669
NdeII GATC 1 cut(s) 186
NlaIII CATG 1 cut(s) 643
NmeAIII GCCGAG 1 cut(s) 49
OliI CACNNNNGTG 1 cut(s) 467
PkrI GCNGC 2 cut(s) 70, 234
Psp124BI GAGCTC 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 12
PspFI CCCAGC 1 cut(s) 228
PspGI CCWGG 1 cut(s) 12
PspPI GGNCC 1 cut(s) 226
PstI CTGCAG 1 cut(s) 70
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 279, 306
SatI GCNGC 2 cut(s) 69, 233
Sau3AI GATC 1 cut(s) 186
Sau96I GGNCC 1 cut(s) 226
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 1 cut(s) 33
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 467
Sse9I AATT 4 cut(s) 115, 400, 510, 677
SsiI CCGC 2 cut(s) 218, 498
SspI AATATT 1 cut(s) 169
SspMI CTAG 1 cut(s) 378
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 2 cut(s) 18, 327
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 2 cut(s) 237, 296
TasI AATT 4 cut(s) 115, 400, 510, 677
Tru1I TTAA 2 cut(s) 279, 306
Tru9I TTAA 2 cut(s) 279, 306
TscAI CASTG 1 cut(s) 469
TseI GCWGC 2 cut(s) 68, 232
TspDTI ATGAA 2 cut(s) 442, 656
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.