Rorug02G0101400

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003066
Physical Location & Seq
Forward (+)
8597356 .. 8607018
9663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0101400.1

Sequence Viewer

Length: 5466 bp
ATGGGCACCCCTGACAACAAATTATCAGAGATAGTTGACATTTTCAAGTCTTGGATGCCCAGGAGAACCGAGCCAACCAATGTGTCCAGGGACTTTTGGATGCCCGATCACAGCTGTAGGGTATGCTACGACTGTGATTCCCAGTTCACAGTATTTAATCGAAGGCATCATTGCCGCCTTTGTGGCCGAGTTTTCTGCGCTAGGTGTACTGCAAACACTATTCCTGCCCCATCTGATGAGCCAAGAATTGGTAGGGAAGACGGGGAGAGGATTAGGGTCTGTAATTTTTGCTTCAAGCAATGGGAGCAAGGGATAGCAGCAGTTGATAATGGGCCCCCTGAACCTAGCCCTGGCCTCAGTCCATCGCCATCTACAACAAGCTTGGTCAGTACCAACACAAGCTGCACCTACCACAGTAGCAGCAGCACTGTTGGTTCTACCCCTTATTCGACTGGACCTTACCAGCACGTGCCCTATAGTTCTGGTCTTAGCCCCAGACAATCTTGCCAAGATGATTCAGTTACTGGCCTGCAAGACAATGTAACATCTCAGAGGAGCATTAGTTCTGACGCTGCCATGGCAGAGTCGTCTCCCAACCAGTTTGGCTTTTGCATGGACAGGAGTGATGATGAGGATGATGACTATGGTTATCATTCAGATTCAGAATCAAGACATTTCACCCATGCCAATGACTATTATGGTGCAATCAATAACGAGGAGTTTGATAGCGTGTATGAACCACAGAAGGTGCATTCTGATGGAGAAACCACAGATGCAAAAAGTTTGAGCTCCTTTTCACCTGAGAAGTTTGATACACAGAGTGTAGTGGGAACGAAACTTGGGGAAGAATCAGATTATCATATTAATTGTGATGAATGTGAAACTTCTCCATATGAGATGGAGACTACAAATGCAGAACCGGTGGATTTTGAGAATAATGGGCTACTTTGGCTCCCACCAGAGCCTGAGGATGAAGAAGATGACAGAGAAGCTGCCTTATTTGATGATGATGATGATGATGAGGGTGGTGATGGTGGTGGTGGTGGTGGTGGTGGTGGTGCTACAGGGGAGTGGGGATATCTTCATTCTTCAAACAGTTTTGGTGGTGGGGAGTGTCGTACTAGGGAGAAGTCAATTGAGGAGCACAGGAAGGCCATGAAGAATGTGGTGGAAGGTCATTTTAGAGCTCTAGTATCTCAGCTCTTGCAGGTTGAAAACCTGCCTTCGATTGATGAAAATTACAAAGAGACTTGGTTGGACATAATTACGTCTTTGTCATGGGAAGCTGCCACACTTCTTAAGCCAGATACGAGCAAAGGGGGAGGAATGGATCCTGGGGGATATGTGAAGGTTAAATGCATAGCTTGTGGGAGTCGGAGCGAGAGCATGGTGGTTAAAGGAGTTGTTTGCAAGAAAAACGTGGCTCATCGGCGAATGACTTCAAAGATAGAAAAACCACGTTTCTTGATCCTTGGAGGAGCTTTGGAATATCAGCGTGTATCAAACCACTTGTCCAGTTTCGATACTTTGTTGCAGCAGGAGATGGACCATCTGAAGATGGCAGTTGCTAAGATCGATGCACATCACCCTAATGTTCTATTGGTGGAGAAGTCTGTATCTCGGTATGCTCAAGATTACCTTCTTGCGAAAGACATATCACTCGTTCTGAATATTAAGAGGCCACTTTTGGAGCGAATATCTCGCTGCACTGGGGCACAAATTGTCCCTTCGATAGATCATCTTACATCACCAAAACTAGGGTATTGCGACATGTTCCATGTGGAGAAATTTTTTGAAGTGCATGGCAGTGCTGGACAAGGTGGGAAGAAATTAACGAAGACTCTGATGTTCTTTGAAGGTTGTCCAAAGCCATTGGGTGTTACTATATTGCTGAAGGGTGCCAATGGGGATGAGTTAAAAAAGGTGAAACATGTTGTGCAGTATGGAGTTTTTGCAGCTTATCACTTGGCTTTGGAGACCTCATTTTTAGCTGATGAAGGAGCTTCCCTTCCAGAACTTCCTCTGAAGTCTGTGGTAACTGTTTTGCCTGATAAACCGTCTAGTATTGACAGGTCCATTTCCACCATACCTGGTTTTAGTGTACCAGCAGCTGGCAAGCCTCAGAGTTCCAATGCTAGCAGTGAACTGCAGAACTCAAATAAAGGCCTCATTTCAGATAGTGGCTCATTTGCCCAAGTTGCCTCCATTTTAAAGAACGAAGGCACCAATTTGGTCTGCTTACCTAAAGCTCCGTGTTCTCAACCTTCCCCCATAAAACATACCCCGAGTCCTATAGAATATACATCTTCTTTCACTTCCTTGTCTCCCCCAGGACAGGATACGACAGACTCCTGCCATAAAGAGCTTTCTTCAATTTGTGGCTCTGAGGACAACAAGGATGTAAGTTCTAAAGAATCTTGTCCAGCCAAAACAGCCAAGGCAGGCGAAGCTTTAGTTCACGATAGTTTGATCTCTAATTCTTTTAGCACCTCAGAGGCATTTGGACATGGTGGTGGAATTGGCAATGCTGATGGCGTTGCTTTAGCTGCAAATCTCAGAGAGACTCCAGAGCTTCCATCCATAAAATATCATACTGATAATCAGAATGAGGAGGTGGGATCTTCAAAAGAAGAGTTCCCCCCCTCACCTTCAGACCACCAGAGCATTTTGGTTTCCCTATCAACACGTTGTGTGTGGAAAGGAACTGTATGTGAACGGGCCCATCTTTTTCGAATCAAATACTATGGAAGCTTTGATAAGCCTCTGGGGAGGTTTTTACGAGACCATCTTTTTGATCAGGGTTACCTCTGTCGTTCATGTGGGATGCCCTCAGAAGCGCATATTCATTGTTATACTCATAGACAAGGAAGCCTTACAATATCTGTTAAGAAACTCCCAGAGATTTTCCTGCCAGGGGAAAAGGAAGGAAAAATATGGATGTGGCATAGGTGCTTGAGATGTCCTCGGACCAGTGGCTTCCCTCCAGCCACTCGTAGAGTTGTAATGTCAGATGCTGCTTGGGGTTTATCTTTTGGGAAATTTCTGGAATTGAGCTTTTCAAATCATGCAGCTGCAAACAGAGTTGCAAGCTGTGGTCATTCCCTGCACAGAGATTGTCTTCGGTTTTATGGGTTTGGGAGAATGGTTGCTTGCTTCCGCTATGCCTCAATTCATATTCACTCTGTTTGTCTGCCCCCACCAAAACTTGAATTTTACTATGATAATCAGGAATGGATACAAAAAGAAGCATATGAGGTGGGCAACCGGGTGGAGCTTCTATTTACTGAATTACATAGTGCTCTTCATCAGATTTTACAGAAGATAAAAGTTGCGGAGACACAGGATGTTGGTAAGAAAGCACCTGAATCGACTCATCAAATTGTAGAACTTGAAGGGATGCTTCAAAAGGAAAGGGAAGACTTTGAGAAATCATTACAAAAAGTGATAAAGGGGGAGGTGAAATCTGGCCAGCCTGCAATTGATATTCTTGAGATCAATAAATTGCGGAGGCAGTTACTCTTCCATTCTTATGTTTGGGACCAGCGCCTGATTCGTGCAGCCAGTCTAAGCAATATTAAATTTCAGGAAGGTTTAACCAGTTCCGTTACCAAACTCAAGGAGAAACCTATTGGCATGGAGAAGGTTGTCAAAACACCAGGAAGAAATATCAGTAGTTGTACAGCTCTTCCCGAAATCAAGCCTGGTATAAATCTTAATCAAGGAGGAGATGCTGGCTACTTGAGCCAGATGGGAGGGGTTCAGAACAGAACTGAAATGGGTCTGGACACAGATCATGGGAATGAGACCTCTGCAAATGTCCTTGATAAATCTGATCCTTTGGAGTCTGAAAAAATTGTACAAATGGGTCTCTCGGAAGAGAATGAATGTTCAGCTGTGGAAAGTTTATCTGATACCCTTGATGCGGCTTGGACTGGTACAACCCACCCAACTAGTACAACATCTAGAGAGAATGGCTATTCACTTCCTCATCCAACCTTGGTAAAATCATCAACTGTGGTAAAAATTGTTGCATCCGCTGTGGAAAATGGTACTGTTGACCAAGGTGGGGTACAGGCAACTCGTTCTCTTATTTCTACATTTCCTGCTATCACTAGTTCATTCAGTAAAAGTGCTTCCTTTAACACTCAAAAGCTATGTATTGGTGGTGAGCACAGTCCTGTCTATGTTGCACGGTTCCGGGAACTGGAACGGCATACTGGTGCCAGGTTGCTATTGCCGATTGGTGTTAATGATACAGTTATCCCTGTTTTTGATGATGAGCCCACTAGTGTTATTGCATATACCCTCGTCTCTCCTAATTATCATTTGCAGATTTCTGAGCCTGATAGACCAAAAGAAGCTATTGATTCTGCAGTCTCATTACCGCTTTTTGATTCAGCGAATCTTCTCTCACTGAATGCTTTTGATGAGTCAGTTTCTGAGACCTACAGAGGTCTTAGTTCTTCTGATGAGGGCATCTTATCCATGTCACATTCGCGGAGCTCTGAATCTCTTGTGTCAAAGGATATACATGCTAGAGTATCCTTTACAGATGAGGGGCCCCTCGGTAAGGTGAAGTATACAGTGACTTGTTACTACGCATCGCAGTTTGAGGCATTAAGGAAGGCATGTTGCCCTTCCGAATTAGATTTTGTGCGGTCTCTTAGCCGCTGTAAGAAGTGGGGTGCCCAGGGTGGAAAGAGCAACGTTTTCTTTGCTAAAACCTTGGATGATCGGTTTATCATCAAACAAGTCACAAAGACGGAGCTGGAGTCATTTATCAAGTTTGCACCAGCTTATTTCAAATATTTGTCTGATTCAATCAGTACTAGAAGCCCAACTTGCCTAGCAAAGATCCTGGGTATCTATCAGGTTTCATCAAAGCATGGTAAAGCAGGGAAAGAGACAAAAATGGATGTCTTAGTAATGGAGAATCTATTATTTAGACGGAATGTCTCACGGCTCTATGACCTGAAAGGATCTTCTCGATCACGTTATAATCCAGATACAAGTGGGAGCAATAAAGTGCTACTGGACCAGAATCTGATTGAAGCAATGCCTACTTCTCCAATTTTTGTGGGGAACAGGGCAAAGCGGCTATTGGAGAGAGCTGTCTGGAATGATACTGCATTCCTTGCTTCAATCGATGTGATGGACTACTCATTACTGGTTGGAGTGGATGAGGAAAAGCACGAGCTAGTTTTGGGGATCATTGATTTCATGAGGCAATATACATGGGACAAGCACCTTGAAACTTGGGTGAAGGCCTCAGGCATCCTCGGAGGACCGAAGAATACATCTCCAACCGTGATTTCACCCCAGCAGTATAAGAAACGTTTCAGGAAAGCTATGGCCACTTACTTTCTGATGCTTCCCGATCAGTGGTCTCCTGAGGCGATTGTCCCAAGTGGGTCGCAATCTGACCATTTTGAAGAAACTTCCCAAGCTCAATCTGAAGCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF2A

Protein Analysis

1821

Amino Acids

200.81

Weight (kDa)

5.82

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 33 - 102 3.5e-17 FYVE zinc finger
Cpn60_TCP1 PF00118 415 - 664 1.2e-30 TCP-1/cpn60 chaperonin family
PIP5K PF01504 1564 - 1730 2.1e-34 Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K PF01504 1729 - 1785 6.7e-08 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4998
Acc36I ACCTGC 2 cut(s) 1198, 1227
AccB1I GGYRCC 5 cut(s) 5, 1898, 2222, 4220, 4685
AccB7I CCANNNNNTGG 2 cut(s) 248, 2111
AccI GTMKAC 1 cut(s) 4580
AccII CGCG 1 cut(s) 4498
AclI AACGTT 2 cut(s) 4707, 5335
AclWI GGATC 8 cut(s) 1325, 1338, 1462, 2626, 3827, 4849, 4987, 5216
AcoI YGGCCR 3 cut(s) 184, 3466, 5352
AcsI RAATTY 4 cut(s) 1787, 3038, 3209, 3578
AcuI CTGAAG 4 cut(s) 1574, 1913, 2045, 2634
AcvI CACGTG 1 cut(s) 469
AdeI CACNNNGTG 2 cut(s) 821, 2690
AflII CTTAAG 1 cut(s) 1298
AflIII ACRYGT 3 cut(s) 1770, 1930, 2684
AgeI ACCGGT 1 cut(s) 919
AhdI GACNNNNNGTC 1 cut(s) 4952
AhlI ACTAGT 3 cut(s) 3950, 4112, 4286
AjuI GAANNNNNNNTTGG 2 cut(s) 5296, 5328
AloI GAACNNNNNNTCC 2 cut(s) 547, 579
Alw21I GWGCWC 6 cut(s) 791, 1146, 1189, 3301, 4173, 4505
AlwI GGATC 8 cut(s) 1325, 1338, 1462, 2626, 3827, 4849, 4987, 5216
AlwNI CAGNNNCTG 7 cut(s) 524, 965, 2111, 3014, 3547, 4439, 5044
Ama87I CYCGRG 1 cut(s) 2284
ApaI GGGCCC 3 cut(s) 336, 2722, 4563
ApoI RAATTY 4 cut(s) 1787, 3038, 3209, 3578
ArsI GACNNNNNNTTYG 4 cut(s) 1496, 1528, 2774, 2806
AseI ATTAAT 1 cut(s) 864
AsiGI ACCGGT 1 cut(s) 919
Asp700I GAANNNNTTC 2 cut(s) 1438, 5336
AspLEI GCGC 3 cut(s) 200, 2839, 3546
AsuC2I CCSGG 2 cut(s) 3266, 4199
AsuII TTCGAA 1 cut(s) 2731
AsuNHI GCTAGC 1 cut(s) 2135
AvaI CYCGRG 1 cut(s) 2284
AvaII GGWCC 7 cut(s) 455, 1546, 2073, 2967, 3538, 5035, 5285
AxyI CCTNAGG 3 cut(s) 966, 5269, 5391
BaeGI GKGCMC 7 cut(s) 8, 336, 474, 1717, 2722, 4563, 4690
BalI TGGCCA 2 cut(s) 3468, 5354
BamHI GGATCC 1 cut(s) 1330
BanI GGYRCC 5 cut(s) 5, 1898, 2222, 4220, 4685
BanII GRGCYC 7 cut(s) 336, 791, 1189, 2722, 4284, 4505, 4563
BarI GAAGNNNNNNTAC 2 cut(s) 4117, 4149
BauI CACGAG 1 cut(s) 5192
BbrPI CACGTG 1 cut(s) 469
BbsI GAAGAC 4 cut(s) 264, 1844, 3110, 3423
Bbv12I GWGCWC 6 cut(s) 791, 1146, 1189, 3301, 4173, 4505
BceAI ACGGC 2 cut(s) 4226, 4976
BcgI CGANNNNNNTGC 4 cut(s) 177, 211, 3348, 3382
BciVI GTATCC 3 cut(s) 2332, 3228, 4552
BclI TGATCA 1 cut(s) 2794
BcnI CCSGG 2 cut(s) 3266, 4199
BcuI ACTAGT 3 cut(s) 3950, 4112, 4286
BfmI CTRYAG 7 cut(s) 115, 475, 1062, 2147, 2292, 4371, 4447
BfoI RGCGCY 1 cut(s) 3547
BfrI CTTAAG 1 cut(s) 1298
BfuAI ACCTGC 2 cut(s) 1198, 1227
BfuI GTATCC 3 cut(s) 2332, 3228, 4552
BglI GCCNNNNNGGC 1 cut(s) 183
BmcAI AGTACT 1 cut(s) 4828
Bme18I GGWCC 7 cut(s) 455, 1546, 2073, 2967, 3538, 5035, 5285
BmeRI GACNNNNNGTC 1 cut(s) 4952
BmeT110I CYCGRG 1 cut(s) 2284
BmrI ACTGGG 2 cut(s) 136, 1719
BmtI GCTAGC 1 cut(s) 2139
BmuI ACTGGG 2 cut(s) 136, 1719
BpiI GAAGAC 4 cut(s) 264, 1844, 3110, 3423
BplI GAGNNNNNCTC 2 cut(s) 2947, 2979
BpmI CTGGAG 3 cut(s) 2550, 2967, 4790
Bpu14I TTCGAA 1 cut(s) 2731
BpuEI CTTGAG 5 cut(s) 1614, 2974, 3509, 3599, 3760
BpuMI CCSGG 2 cut(s) 3266, 4199
Bsa29I ATCGAT 2 cut(s) 1575, 5145
BsaAI YACGTR 1 cut(s) 469
BsaBI GATNNNNATC 1 cut(s) 1581
BsaI GGTCTC 7 cut(s) 1970, 2775, 3797, 3872, 4436, 4665, 5391
BsaWI WCCGGW 1 cut(s) 919
BsaXI ACNNNNNCTCC 6 cut(s) 547, 577, 936, 966, 4757, 4787
Bse118I RCCGGY 1 cut(s) 919
Bse21I CCTNAGG 3 cut(s) 966, 5269, 5391
Bse3DI GCAATG 4 cut(s) 169, 305, 2530, 5061
Bse8I GATNNNNATC 1 cut(s) 1581
BseCI ATCGAT 2 cut(s) 1575, 5145
BseJI GATNNNNATC 1 cut(s) 1581
BseMI GCAATG 4 cut(s) 169, 305, 2530, 5061
BseRI GAGGAG 6 cut(s) 568, 731, 1154, 1491, 2624, 3738
BseSI GKGCMC 7 cut(s) 8, 336, 474, 1717, 2722, 4563, 4690
BseYI CCCAGC 1 cut(s) 5319
BsgI GTGCAG 5 cut(s) 388, 1690, 1958, 3089, 3576
Bsh1236I CGCG 1 cut(s) 4498
BshNI GGYRCC 5 cut(s) 5, 1898, 2222, 4220, 4685
BshTI ACCGGT 1 cut(s) 919
BshVI ATCGAT 2 cut(s) 1575, 5145
BsiHKAI GWGCWC 6 cut(s) 791, 1146, 1189, 3301, 4173, 4505
BsiHKCI CYCGRG 1 cut(s) 2284
BsiSI CCGG 3 cut(s) 920, 3265, 4198
BslFI GGGAC 5 cut(s) 104, 1709, 3551, 5252, 5387
BsmBI CGTCTC 2 cut(s) 594, 4315
BsmFI GGGAC 5 cut(s) 104, 1709, 3551, 5252, 5387
BsmI GAATGC 3 cut(s) 751, 4423, 5129
Bso31I GGTCTC 7 cut(s) 1970, 2775, 3797, 3872, 4436, 4665, 5391
BsoBI CYCGRG 1 cut(s) 2284
Bsp119I TTCGAA 1 cut(s) 2731
Bsp120I GGGCCC 3 cut(s) 332, 2718, 4559
Bsp1407I TGTACA 2 cut(s) 3677, 3856
Bsp19I CCATGG 1 cut(s) 576
BspDI ATCGAT 2 cut(s) 1575, 5145
BspFNI CGCG 1 cut(s) 4498
BspHI TCATGA 1 cut(s) 5220
BspMAI CTGCAG 2 cut(s) 2151, 4375
BspMI ACCTGC 2 cut(s) 1198, 1227
BspOI GCTAGC 1 cut(s) 2139
BspPI GGATC 8 cut(s) 1325, 1338, 1462, 2626, 3827, 4849, 4987, 5216
BspQI GCTCTTC 2 cut(s) 3306, 3690
BspT104I TTCGAA 1 cut(s) 2731
BspT107I GGYRCC 5 cut(s) 5, 1898, 2222, 4220, 4685
BspTI CTTAAG 1 cut(s) 1298
BspTNI GGTCTC 7 cut(s) 1970, 2775, 3797, 3872, 4436, 4665, 5391
BsrDI GCAATG 4 cut(s) 169, 305, 2530, 5061
BsrFI RCCGGY 1 cut(s) 919
BsrGI TGTACA 2 cut(s) 3677, 3856
BssAI RCCGGY 1 cut(s) 919
BssNAI GTATAC 1 cut(s) 4581
BssSI CACGAG 1 cut(s) 5192
BssT1I CCWWGG 6 cut(s) 576, 1471, 2436, 3996, 4060, 4725
Bst1107I GTATAC 1 cut(s) 4581
Bst2BI CACGAG 1 cut(s) 5192
Bst6I CTCTTC 5 cut(s) 2625, 3306, 3524, 3690, 3870
BstAFI CTTAAG 1 cut(s) 1298
BstAPI GCANNNNNTGC 1 cut(s) 5135
BstAUI TGTACA 2 cut(s) 3677, 3856
BstBAI YACGTR 1 cut(s) 469
BstBI TTCGAA 1 cut(s) 2731
BstDSI CCRYGG 1 cut(s) 576
BstEII GGTNACC 1 cut(s) 2801
BstENI CCTNNNNNAGG 1 cut(s) 4568
BstFNI CGCG 1 cut(s) 4498
BstH2I RGCGCY 1 cut(s) 3547
BstHHI GCGC 3 cut(s) 200, 2839, 3546
BstNSI RCATGY 4 cut(s) 1774, 1934, 4535, 4632
BstPI GGTNACC 1 cut(s) 2801
BstSFI CTRYAG 7 cut(s) 115, 475, 1062, 2147, 2292, 4371, 4447
BstSLI GKGCMC 7 cut(s) 8, 336, 474, 1717, 2722, 4563, 4690
BstUI CGCG 1 cut(s) 4498
BstV2I GAAGAC 4 cut(s) 264, 1844, 3110, 3423
BstX2I RGATCY 4 cut(s) 1330, 2618, 4854, 4979
BstYI RGATCY 4 cut(s) 1330, 2618, 4854, 4979
BstZ17I GTATAC 1 cut(s) 4581
Bsu15I ATCGAT 2 cut(s) 1575, 5145
Bsu36I CCTNAGG 3 cut(s) 966, 5269, 5391
BsuI GTATCC 3 cut(s) 2332, 3228, 4552
BsuTUI ATCGAT 2 cut(s) 1575, 5145
BtgI CCRYGG 1 cut(s) 576
BtgZI GCGATG 2 cut(s) 348, 4587
BtsI GCAGTG 2 cut(s) 1813, 2146
BtsIMutI CAGTG 8 cut(s) 426, 1707, 1813, 2146, 2977, 4412, 4590, 5387
BveI ACCTGC 2 cut(s) 1198, 1227
CaiI CAGNNNCTG 7 cut(s) 524, 965, 2111, 3014, 3547, 4439, 5044
CciI TCATGA 1 cut(s) 5220
CfoI GCGC 3 cut(s) 200, 2839, 3546
Cfr10I RCCGGY 1 cut(s) 919
ClaI ATCGAT 2 cut(s) 1575, 5145
CseI GACGC 1 cut(s) 578
CsiI ACCWGGT 1 cut(s) 2089
CspAI ACCGGT 1 cut(s) 919
CspCI CAANNNNNGTGG 4 cut(s) 1446, 1481, 5058, 5093
DraI TTTAAA 1 cut(s) 2211
DraIII CACNNNGTG 2 cut(s) 821, 2690
DriI GACNNNNNGTC 1 cut(s) 4952
EaeI YGGCCR 3 cut(s) 184, 3466, 5352
Eam1104I CTCTTC 5 cut(s) 2625, 3306, 3524, 3690, 3870
Eam1105I GACNNNNNGTC 1 cut(s) 4952
EarI CTCTTC 5 cut(s) 2625, 3306, 3524, 3690, 3870
Ecl136II GAGCTC 3 cut(s) 789, 1187, 4503
Eco130I CCWWGG 6 cut(s) 576, 1471, 2436, 3996, 4060, 4725
Eco147I AGGCCT 2 cut(s) 2166, 5267
Eco24I GRGCYC 7 cut(s) 336, 791, 1189, 2722, 4284, 4505, 4563
Eco31I GGTCTC 7 cut(s) 1970, 2775, 3797, 3872, 4436, 4665, 5391
Eco32I GATATC 1 cut(s) 1079
Eco47I GGWCC 7 cut(s) 455, 1546, 2073, 2967, 3538, 5035, 5285
Eco53kI GAGCTC 3 cut(s) 789, 1187, 4503
Eco57I CTGAAG 4 cut(s) 1574, 1913, 2045, 2634
Eco72I CACGTG 1 cut(s) 469
Eco81I CCTNAGG 3 cut(s) 966, 5269, 5391
Eco88I CYCGRG 1 cut(s) 2284
Eco91I GGTNACC 1 cut(s) 2801
EcoICRI GAGCTC 3 cut(s) 789, 1187, 4503
EcoNI CCTNNNNNAGG 1 cut(s) 4568
EcoO109I RGGNCCY 3 cut(s) 333, 4559, 4560
EcoO65I GGTNACC 1 cut(s) 2801
EcoRV GATATC 1 cut(s) 1079
EcoT14I CCWWGG 6 cut(s) 576, 1471, 2436, 3996, 4060, 4725
EcoT22I ATGCAT 1 cut(s) 1361
EcoT38I GRGCYC 7 cut(s) 336, 791, 1189, 2722, 4284, 4505, 4563
ErhI CCWWGG 6 cut(s) 576, 1471, 2436, 3996, 4060, 4725
Esp3I CGTCTC 2 cut(s) 594, 4315
FalI AAGNNNNNCTT 8 cut(s) 1623, 1655, 2856, 2888, 3384, 3416, 4825, 4857
FaqI GGGAC 5 cut(s) 104, 1709, 3551, 5252, 5387
FauNDI CATATG 2 cut(s) 892, 3250
FbaI TGATCA 1 cut(s) 2794
FblI GTMKAC 1 cut(s) 4580
FriOI GRGCYC 7 cut(s) 336, 791, 1189, 2722, 4284, 4505, 4563
GlaI GCGC 3 cut(s) 199, 2838, 3545
GsaI CCCAGC 1 cut(s) 5323
GsuI CTGGAG 3 cut(s) 2550, 2967, 4790
HaeII RGCGCY 1 cut(s) 3547
HapII CCGG 3 cut(s) 920, 3265, 4198
HgaI GACGC 1 cut(s) 578
HhaI GCGC 3 cut(s) 200, 2839, 3546
Hin6I GCGC 3 cut(s) 198, 2837, 3544
HinP1I GCGC 3 cut(s) 198, 2837, 3544
HincII GTYRAC 2 cut(s) 37, 4057
HindII GTYRAC 2 cut(s) 37, 4057
HindIII AAGCTT 3 cut(s) 379, 2448, 2749
HpaII CCGG 3 cut(s) 920, 3265, 4198
Hpy166II GTNNAC 9 cut(s) 37, 147, 207, 2102, 2144, 2458, 2714, 4057, 4581
Hpy8I GTNNAC 9 cut(s) 37, 147, 207, 2102, 2144, 2458, 2714, 4057, 4581
HpyCH4IV ACGT 8 cut(s) 468, 1268, 1419, 1459, 2686, 4707, 4993, 5335
HpySE526I ACGT 8 cut(s) 468, 1268, 1419, 1459, 2686, 4707, 4993, 5335
HspAI GCGC 3 cut(s) 198, 2837, 3544
Ksp22I TGATCA 1 cut(s) 2794
LguI GCTCTTC 2 cut(s) 3306, 3690
MabI ACCWGGT 1 cut(s) 2089
MaeII ACGT 8 cut(s) 468, 1268, 1419, 1459, 2686, 4707, 4993, 5335
MfeI CAATTG 2 cut(s) 1134, 3477
MflI RGATCY 4 cut(s) 1330, 2618, 4854, 4979
MlsI TGGCCA 2 cut(s) 3468, 5354
MluNI TGGCCA 2 cut(s) 3468, 5354
MmeI TCCRAC 5 cut(s) 1236, 1355, 4016, 5152, 5327
Mox20I TGGCCA 2 cut(s) 3468, 5354
Mph1103I ATGCAT 1 cut(s) 1361
MroXI GAANNNNTTC 2 cut(s) 1438, 5336
MscI TGGCCA 2 cut(s) 3468, 5354
MslI CAYNNNNRTG 8 cut(s) 687, 756, 1590, 1806, 2511, 3528, 3798, 4218
Msp20I TGGCCA 2 cut(s) 3468, 5354
MspA1I CMGCKG 6 cut(s) 114, 2111, 3071, 3893, 4037, 4671
MspCI CTTAAG 1 cut(s) 1298
MspI CCGG 3 cut(s) 920, 3265, 4198
MunI CAATTG 2 cut(s) 1134, 3477
Mva1269I GAATGC 3 cut(s) 751, 4423, 5129
MvnI CGCG 1 cut(s) 4498
NciI CCSGG 2 cut(s) 3266, 4199
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 2 cut(s) 892, 3250
NheI GCTAGC 1 cut(s) 2135
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 3 cut(s) 4488, 4585, 4753
NsiI ATGCAT 1 cut(s) 1361
NspI RCATGY 4 cut(s) 1774, 1934, 4535, 4632
NspV TTCGAA 1 cut(s) 2731
PagI TCATGA 1 cut(s) 5220
PasI CCCWGGG 1 cut(s) 4690
PceI AGGCCT 2 cut(s) 2166, 5267
PciI ACATGT 2 cut(s) 1770, 1930
PciSI GCTCTTC 2 cut(s) 3306, 3690
PctI GAATGC 3 cut(s) 751, 4423, 5129
PdmI GAANNNNTTC 2 cut(s) 1438, 5336
PflMI CCANNNNNTGG 2 cut(s) 248, 2111
PfoI TCCNGGA 1 cut(s) 4197
PinAI ACCGGT 1 cut(s) 919
PmaCI CACGTG 1 cut(s) 469
PmlI CACGTG 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 469
PscI ACATGT 2 cut(s) 1770, 1930
PshBI ATTAAT 1 cut(s) 864
PsiI TTATAA 1 cut(s) 4998
Psp124BI GAGCTC 3 cut(s) 791, 1189, 4505
Psp1406I AACGTT 2 cut(s) 4707, 5335
PspCI CACGTG 1 cut(s) 469
PspEI GGTNACC 1 cut(s) 2801
PspFI CCCAGC 1 cut(s) 5319
PspOMI GGGCCC 3 cut(s) 332, 2718, 4559
PstI CTGCAG 2 cut(s) 2151, 4375
PstNI CAGNNNCTG 7 cut(s) 524, 965, 2111, 3014, 3547, 4439, 5044
PsuI RGATCY 4 cut(s) 1330, 2618, 4854, 4979
PvuII CAGCTG 4 cut(s) 114, 2111, 3071, 3893
RseI CAYNNNNRTG 8 cut(s) 687, 756, 1590, 1806, 2511, 3528, 3798, 4218
SacI GAGCTC 3 cut(s) 791, 1189, 4505
SapI GCTCTTC 2 cut(s) 3306, 3690
ScaI AGTACT 1 cut(s) 4828
SexAI ACCWGGT 1 cut(s) 2089
SfcI CTRYAG 7 cut(s) 115, 475, 1062, 2147, 2292, 4371, 4447
SfuI TTCGAA 1 cut(s) 2731
SinI GGWCC 7 cut(s) 455, 1546, 2073, 2967, 3538, 5035, 5285
SmiMI CAYNNNNRTG 8 cut(s) 687, 756, 1590, 1806, 2511, 3528, 3798, 4218
SmlI CTYRAG 6 cut(s) 1298, 1629, 2953, 3488, 3614, 3739
SmoI CTYRAG 6 cut(s) 1298, 1629, 2953, 3488, 3614, 3739
SpeI ACTAGT 3 cut(s) 3950, 4112, 4286
SseBI AGGCCT 2 cut(s) 2166, 5267
SspI AATATT 3 cut(s) 1672, 3574, 4808
SstI GAGCTC 3 cut(s) 791, 1189, 4505
StuI AGGCCT 2 cut(s) 2166, 5267
StyI CCWWGG 6 cut(s) 576, 1471, 2436, 3996, 4060, 4725
TaiI ACGT 8 cut(s) 471, 1271, 1422, 1462, 2689, 4710, 4996, 5338
TaqII GACCGA 1 cut(s) 5302
TatI WGTACW 5 cut(s) 206, 3677, 3856, 3953, 4826
TauI GCSGC 4 cut(s) 177, 3926, 4671, 5098
TscAI CASTG 8 cut(s) 433, 1714, 1813, 2146, 2977, 4419, 4590, 5387
TseFI GTSAC 3 cut(s) 4488, 4585, 4753
Tsp45I GTSAC 3 cut(s) 4488, 4585, 4753
TspGWI ACGGA 4 cut(s) 2241, 3592, 4778, 4963
TspRI CASTG 8 cut(s) 433, 1714, 1813, 2146, 2977, 4419, 4590, 5387
Van91I CCANNNNNTGG 2 cut(s) 248, 2111
Vha464I CTTAAG 1 cut(s) 1298
VpaK11BI GGWCC 7 cut(s) 455, 1546, 2073, 2967, 3538, 5035, 5285
VspI ATTAAT 1 cut(s) 864
XagI CCTNNNNNAGG 1 cut(s) 4568
XapI RAATTY 4 cut(s) 1787, 3038, 3209, 3578
XbaI TCTAGA 1 cut(s) 3962
XceI RCATGY 4 cut(s) 1774, 1934, 4535, 4632
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XmiI GTMKAC 1 cut(s) 4580
XmnI GAANNNNTTC 2 cut(s) 1438, 5336
ZrmI AGTACT 1 cut(s) 4828
Zsp2I ATGCAT 1 cut(s) 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.