FvH4_5g29730

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003239
Physical Location & Seq
Forward (+)
20655111 .. 20661512
6402 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29730.t3

Sequence Viewer

Length: 1749 bp
ATGATGGAGGCGCCGCCATTGGTTTCTCCTACCAAGACGCCGCCGTTGCGGTCAGCCTCCTCTTCCTCCTCTCTTCTCTTTCTCCGCAAGCATTCTCTCAATTGCCGCCGACCTTCCAGAAGCTTCGCCGTCCGCGCCTCCGCTGCTACCGATTCAGTGGTGACTTTGCTTGATTACGGCGCCGGAAATGTTCGTAGTGTCAGGAATGCCATCCGCCGCCTCGGTTTCAAAGTCAGGGATGTTCAAACTCCGAAAGATATTCTGAATGCCAACCGCCTGATCTTTCCTGGTGTGGGGGCTTTTGCTGCCGCTATGGATGTGCTGAGCAAGAACGGGCTGGCAGAAGCTCTCTGTGAATATATTGAGAATGATCGGCCGTTTCTAGGCATTTGTCTTGGACTTCAGCTCCTTTTTGAATCCAGTGAGGAAAAGGGACAAGTGAGTGGTCTTGGCTTGATACCTGGAGTGGTTGGGCGTTTTGACTCATCAAATGGTGTCAGAGTGCCACATATCGGGTGGAATGCTTTGCAGATTAAAAAAGACTCATCAATTTTGGATGATGTTGGAGCCCATCATGTCTATTTCGTTCACTCTTACAGAGCGATGCCATCGGATGAAAACAAAGAATGGGTTTCATCTACCTGCAACTATGGTGACAATTTCATTGCATCTGTTAGGAGGGGAAATGTGCATGCAGTTCAATTCCACCCCGAAAAGAGCGGAGATGTTGGCCTTTCCATATTGAGAAGATTTCTCTACCCAAACTCACCCTTGGAGAAGAAGCCCACTGAAGGGAAGGGTTCCAAACTTGCAAAGAGGGTTATTGCTTGTCTGGATGTGAGGACGAATGATAAAGGAGATCTAGTGGTAACCAAAGGAGACCAGTACGATGTAAGAGAGCATACAAAGGAGAATGAGGTGAGAGAACTTGGCAAGCCTGTGGAGCTTGCTGGGCAGTATTACACAGATGGAGCTGATGAGGTCAGCTTTCTGAATATTACCGGTTTTCGGGACTTCCCTTTGGGTGACTTGCCTATGCTTAAGGTATTAAGATATGCATCAGAAAATGTTTTTGTGCCATTGACAGTTGGAGGTGGCATTAGAGATTTTACCGATGCAACTGGCAGGCACTATTCGAGCTTGGAAGTTGCTTCAGAATATTTTAGATGTGGGGCTGATAAGATCTCCATTGGAAGTGATGCAGTTTATGCTGCAGAAGAATATCTAAGAACTGGCGTGAAAAGTGGAAAAAGTAGCTTAGAGCAGATATCTAGAGTTTACGGAAATCAGGCTGTGGTTGTTAGCATTGATCCTCGCAGAGTGTACGTCAAAAATCAAAATGATGTTAAGTTCAAGACTATAAGAGTAACAAACCCAGGTCCAAATGATGAAGAATATGCTTGGTATCAGTGTACAGTTAGTGGTGGGCGGGAAGGCAGACCAATTGGAGCTTATGAGCTAGCAAAAGCAGTTGAGGAGCTTGGAGCTGGAGAAATTCTGCTCAATTGCATTGATTGTGATGGTCAAGGGAAGGGATTTGATATAGATTTAGTAAAGCTGATCTCTGATGCTGTGACCATTCCCGTGATTGCAAGTAGTGGTGCTGGTGCTGTTGAACACTTCTCAGAGGTTTTCACGAAAACAAATGCATCTGCTGCCCTTGCAGCTGGAATTTTCCATCGCAAGGAGGTACCAATTCGTTCTGTAAAGGAGCACTTGCTAAAGGAAGGCATAGAAGTCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

583

Amino Acids

63.55

Weight (kDa)

7.59

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 56 - 252 8.5e-24 Glutamine amidotransferase class-I
SNO PF01174 62 - 242 2.1e-08 SNO glutamine amidotransferase family
GATase_3 PF07685 77 - 155 5e-08 CobB/CobQ-like glutamine amidotransferase domain
His_biosynth PF00977 273 - 509 4e-24 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 650
Acc65I GGTACC 1 cut(s) 1690
AccB1I GGYRCC 3 cut(s) 10, 179, 1690
AccBSI CCGCTC 1 cut(s) 720
AccII CGCG 1 cut(s) 135
AclWI GGATC 1 cut(s) 1304
AcoI YGGCCR 1 cut(s) 374
AcsI RAATTY 2 cut(s) 1494, 1671
AcuI CTGAAG 3 cut(s) 386, 810, 1137
AcyI GRCGYC 3 cut(s) 11, 38, 180
AfaI GTAC 4 cut(s) 887, 1325, 1414, 1692
AfiI CCNNNNNNNGG 7 cut(s) 293, 383, 512, 791, 792, 1008, 1684
AflII CTTAAG 1 cut(s) 1040
AgeI ACCGGT 1 cut(s) 1001
AgsI TTSAA 6 cut(s) 229, 245, 416, 701, 1354, 1616
AjnI CCWGG 3 cut(s) 286, 460, 1375
Alw21I GWGCWC 1 cut(s) 1716
Alw26I GTCTC 1 cut(s) 873
AlwI GGATC 1 cut(s) 1304
AlwNI CAGNNNCTG 1 cut(s) 1746
AoxI GGCC 2 cut(s) 374, 730
ApeKI GCWGC 5 cut(s) 143, 305, 1211, 1655, 1664
ApoI RAATTY 2 cut(s) 1494, 1671
AsiGI ACCGGT 1 cut(s) 1001
Asp718I GGTACC 1 cut(s) 1690
AspLEI GCGC 3 cut(s) 13, 137, 182
AspS9I GGNCC 1 cut(s) 1379
AsuHPI GGTGA 5 cut(s) 172, 665, 759, 931, 1037
AsuNHI GCTAGC 1 cut(s) 1459
AvaII GGWCC 1 cut(s) 1379
BanI GGYRCC 3 cut(s) 10, 179, 1690
BanII GRGCYC 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 1716
BbvI GCAGC 5 cut(s) 130, 292, 1198, 1642, 1676
BccI CCATC 6 cut(s) 218, 579, 616, 962, 1514, 1686
BceAI ACGGC 4 cut(s) 28, 113, 193, 361
BciT130I CCWGG 3 cut(s) 288, 462, 1377
BcoDI GTCTC 1 cut(s) 873
BfaI CTAG 4 cut(s) 383, 863, 1272, 1460
BfmI CTRYAG 1 cut(s) 1212
BfoI RGCGCY 2 cut(s) 14, 183
BfrI CTTAAG 1 cut(s) 1040
BfuAI ACCTGC 1 cut(s) 650
BglII AGATCT 2 cut(s) 859, 1182
BlpI GCTNAGC 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 288, 462, 1377
Bme18I GGWCC 1 cut(s) 1379
BmgT120I GGNCC 1 cut(s) 1379
BmiI GGNNCC 5 cut(s) 12, 181, 568, 802, 1692
BmrFI CCNGG 3 cut(s) 288, 462, 1377
BmsI GCATC 7 cut(s) 594, 677, 1067, 1105, 1189, 1558, 1658
BmtI GCTAGC 1 cut(s) 1463
BpmI CTGGAG 2 cut(s) 483, 1509
Bpu1102I GCTNAGC 1 cut(s) 323
BsaBI GATNNNNATC 1 cut(s) 1057
BsaHI GRCGYC 3 cut(s) 11, 38, 180
BsaI GGTCTC 1 cut(s) 873
BsaJI CCNNGG 3 cut(s) 220, 771, 1375
BsaWI WCCGGW 1 cut(s) 1001
BsaXI ACNNNNNCTCC 4 cut(s) 390, 420, 849, 879
Bsc4I CCNNNNNNNGG 7 cut(s) 293, 383, 512, 791, 792, 1008, 1684
Bse118I RCCGGY 1 cut(s) 1001
Bse1I ACTGG 4 cut(s) 420, 883, 1126, 1237
Bse3DI GCAATG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 1057
BseBI CCWGG 3 cut(s) 288, 462, 1377
BseDI CCNNGG 3 cut(s) 220, 771, 1375
BseGI GGATG 6 cut(s) 210, 244, 322, 562, 619, 841
BseJI GATNNNNATC 1 cut(s) 1057
BseLI CCNNNNNNNGG 7 cut(s) 293, 383, 512, 791, 792, 1008, 1684
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 2 cut(s) 314, 1638
BseNI ACTGG 4 cut(s) 420, 883, 1126, 1237
BseRI GAGGAG 3 cut(s) 49, 58, 1490
BseX3I CGGCCG 1 cut(s) 374
BseXI GCAGC 5 cut(s) 130, 292, 1198, 1642, 1676
BseYI CCCAGC 1 cut(s) 950
Bsh1236I CGCG 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 377
BshFI GGCC 2 cut(s) 376, 732
BshNI GGYRCC 3 cut(s) 10, 179, 1690
BshTI ACCGGT 1 cut(s) 1001
BsiEI CGRYCG 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 1716
BsiSI CCGG 2 cut(s) 183, 1002
BslFI GGGAC 2 cut(s) 447, 1025
BslI CCNNNNNNNGG 7 cut(s) 293, 383, 512, 791, 792, 1008, 1684
BsmAI GTCTC 1 cut(s) 873
BsmFI GGGAC 2 cut(s) 447, 1025
BsmI GAATGC 4 cut(s) 91, 211, 271, 526
BsnI GGCC 2 cut(s) 376, 732
Bso31I GGTCTC 1 cut(s) 873
Bsp1286I GDGCHC 2 cut(s) 571, 1716
Bsp1407I TGTACA 1 cut(s) 1412
Bsp143I GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
Bsp1720I GCTNAGC 1 cut(s) 323
BspANI GGCC 2 cut(s) 376, 732
BspCNI CTCAG 2 cut(s) 315, 1637
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 5 cut(s) 12, 181, 568, 802, 1692
BspMAI CTGCAG 1 cut(s) 1216
BspMI ACCTGC 1 cut(s) 650
BspOI GCTAGC 1 cut(s) 1463
BspPI GGATC 1 cut(s) 1304
BspT107I GGYRCC 3 cut(s) 10, 179, 1690
BspTI CTTAAG 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 873
BsrBI CCGCTC 1 cut(s) 720
BsrDI GCAATG 1 cut(s) 663
BsrFI RCCGGY 1 cut(s) 1001
BsrGI TGTACA 1 cut(s) 1412
BsrI ACTGG 4 cut(s) 420, 883, 1126, 1237
BssAI RCCGGY 1 cut(s) 1001
BssECI CCNNGG 3 cut(s) 220, 771, 1375
BssMI GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
BssNI GRCGYC 3 cut(s) 11, 38, 180
BssT1I CCWWGG 1 cut(s) 771
Bst2UI CCWGG 3 cut(s) 288, 462, 1377
Bst4CI ACNGT 2 cut(s) 1087, 1417
Bst6I CTCTTC 2 cut(s) 67, 78
BstACI GRCGYC 3 cut(s) 11, 38, 180
BstAFI CTTAAG 1 cut(s) 1040
BstAPI GCANNNNNTGC 2 cut(s) 1208, 1655
BstAUI TGTACA 1 cut(s) 1412
BstC8I GCNNGC 7 cut(s) 89, 339, 693, 935, 948, 1127, 1461
BstDEI CTNAG 4 cut(s) 323, 1226, 1258, 1624
BstEII GGTNACC 1 cut(s) 868
BstF5I GGATG 6 cut(s) 210, 244, 322, 562, 619, 841
BstFNI CGCG 1 cut(s) 135
BstH2I RGCGCY 2 cut(s) 14, 183
BstHHI GCGC 3 cut(s) 13, 137, 182
BstKTI GATC 6 cut(s) 282, 373, 862, 1185, 1312, 1563
BstMAI GTCTC 1 cut(s) 873
BstMBI GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
BstMCI CGRYCG 1 cut(s) 377
BstNI CCWGG 3 cut(s) 288, 462, 1377
BstNSI RCATGY 1 cut(s) 695
BstPI GGTNACC 1 cut(s) 868
BstSCI CCNGG 3 cut(s) 286, 460, 1375
BstSFI CTRYAG 1 cut(s) 1212
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 5 cut(s) 130, 292, 1198, 1642, 1676
BstX2I RGATCY 2 cut(s) 859, 1182
BstYI RGATCY 2 cut(s) 859, 1182
BstZI CGGCCG 1 cut(s) 374
BsuRI GGCC 2 cut(s) 376, 732
BtgZI GCGATG 2 cut(s) 617, 1664
BtsCI GGATG 6 cut(s) 210, 244, 322, 562, 619, 841
BtsIMutI CAGTG 4 cut(s) 162, 427, 786, 1415
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 7 cut(s) 89, 339, 693, 935, 948, 1127, 1461
CaiI CAGNNNCTG 1 cut(s) 1746
CfoI GCGC 3 cut(s) 13, 137, 182
Cfr10I RCCGGY 1 cut(s) 1001
Cfr13I GGNCC 1 cut(s) 1379
CseI GACGC 1 cut(s) 46
Csp6I GTAC 4 cut(s) 886, 1324, 1413, 1691
CspAI ACCGGT 1 cut(s) 1001
CviAII CATG 2 cut(s) 575, 692
CviQI GTAC 4 cut(s) 886, 1324, 1413, 1691
DdeI CTNAG 4 cut(s) 323, 1226, 1258, 1624
DinI GGCGCC 2 cut(s) 12, 181
DpnI GATC 6 cut(s) 281, 372, 861, 1184, 1311, 1562
DpnII GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
EaeI YGGCCR 1 cut(s) 374
EagI CGGCCG 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 67, 78
EarI CTCTTC 2 cut(s) 67, 78
EciI GGCGGA 1 cut(s) 203
EclXI CGGCCG 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 873
Eco32I GATATC 1 cut(s) 1269
Eco47I GGWCC 1 cut(s) 1379
Eco52I CGGCCG 1 cut(s) 374
Eco57I CTGAAG 3 cut(s) 386, 810, 1137
Eco91I GGTNACC 1 cut(s) 868
EcoO65I GGTNACC 1 cut(s) 868
EcoRII CCWGG 3 cut(s) 286, 460, 1375
EcoRV GATATC 1 cut(s) 1269
EcoT14I CCWWGG 1 cut(s) 771
EcoT22I ATGCAT 2 cut(s) 1060, 1651
EcoT38I GRGCYC 1 cut(s) 571
EgeI GGCGCC 2 cut(s) 12, 181
EheI GGCGCC 2 cut(s) 12, 181
ErhI CCWWGG 1 cut(s) 771
FaeI CATG 2 cut(s) 578, 695
FalI AAGNNNNNCTT 2 cut(s) 1700, 1732
FaqI GGGAC 2 cut(s) 447, 1025
FatI CATG 2 cut(s) 574, 691
FauI CCCGC 1 cut(s) 1422
FokI GGATG 6 cut(s) 197, 251, 329, 569, 626, 848
FriOI GRGCYC 1 cut(s) 571
FspBI CTAG 4 cut(s) 383, 863, 1272, 1460
GlaI GCGC 3 cut(s) 12, 136, 181
GsaI CCCAGC 1 cut(s) 954
GsuI CTGGAG 2 cut(s) 483, 1509
HaeII RGCGCY 2 cut(s) 14, 183
HaeIII GGCC 2 cut(s) 376, 732
HapII CCGG 2 cut(s) 183, 1002
HgaI GACGC 1 cut(s) 46
HhaI GCGC 3 cut(s) 13, 137, 182
Hin1I GRCGYC 3 cut(s) 11, 38, 180
Hin1II CATG 2 cut(s) 578, 695
Hin6I GCGC 3 cut(s) 11, 135, 180
HinP1I GCGC 3 cut(s) 11, 135, 180
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 5 cut(s) 152, 416, 482, 542, 1743
HpaII CCGG 2 cut(s) 183, 1002
HphI GGTGA 5 cut(s) 172, 665, 759, 931, 1037
Hpy166II GTNNAC 4 cut(s) 589, 1279, 1324, 1413
Hpy188III TCNNGA 7 cut(s) 117, 202, 833, 1010, 1272, 1354, 1636
Hpy8I GTNNAC 4 cut(s) 589, 1279, 1324, 1413
HpyAV CCTTC 6 cut(s) 123, 785, 790, 1427, 1525, 1721
HpyCH4III ACNGT 2 cut(s) 1087, 1417
HpyCH4IV ACGT 1 cut(s) 1326
HpyF3I CTNAG 4 cut(s) 323, 1226, 1258, 1624
HpySE526I ACGT 1 cut(s) 1326
Hsp92I GRCGYC 3 cut(s) 11, 38, 180
Hsp92II CATG 2 cut(s) 578, 695
HspAI GCGC 3 cut(s) 11, 135, 180
KasI GGCGCC 2 cut(s) 10, 179
KpnI GGTACC 1 cut(s) 1694
Kzo9I GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
LmnI GCTCC 8 cut(s) 411, 566, 943, 971, 1448, 1477, 1484, 1711
Lsp1109I GCAGC 5 cut(s) 130, 292, 1198, 1642, 1676
LweI GCATC 7 cut(s) 594, 677, 1067, 1105, 1189, 1558, 1658
MaeI CTAG 4 cut(s) 383, 863, 1272, 1460
MaeII ACGT 1 cut(s) 1326
MaeIII GTNAC 6 cut(s) 160, 653, 868, 1025, 1366, 1573
MalI GATC 6 cut(s) 281, 372, 861, 1184, 1311, 1562
MbiI CCGCTC 1 cut(s) 720
MboI GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
MboII GAAGA 6 cut(s) 54, 65, 759, 790, 1229, 1403
MfeI CAATTG 3 cut(s) 100, 1443, 1504
MflI RGATCY 2 cut(s) 859, 1182
MhlI GDGCHC 2 cut(s) 571, 1716
MluCI AATT 9 cut(s) 100, 549, 658, 701, 1443, 1494, 1504, 1671, 1695
Mly113I GGCGCC 2 cut(s) 11, 180
MlyI GAGTC 2 cut(s) 476, 536
MmeI TCCRAC 2 cut(s) 544, 1069
Mph1103I ATGCAT 2 cut(s) 1060, 1651
MseI TTAA 4 cut(s) 534, 1041, 1049, 1347
MslI CAYNNNNRTG 1 cut(s) 1583
MspA1I CMGCKG 2 cut(s) 143, 1667
MspCI CTTAAG 1 cut(s) 1040
MspI CCGG 2 cut(s) 183, 1002
MspR9I CCNGG 3 cut(s) 288, 462, 1377
MunI CAATTG 3 cut(s) 100, 1443, 1504
Mva1269I GAATGC 4 cut(s) 91, 211, 271, 526
MvaI CCWGG 3 cut(s) 288, 462, 1377
MvnI CGCG 1 cut(s) 135
NarI GGCGCC 2 cut(s) 11, 180
NdeII GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
NheI GCTAGC 1 cut(s) 1459
NlaIII CATG 2 cut(s) 578, 695
NlaIV GGNNCC 5 cut(s) 12, 181, 568, 802, 1692
NmuCI GTSAC 4 cut(s) 160, 653, 1025, 1573
NsiI ATGCAT 2 cut(s) 1060, 1651
NspI RCATGY 1 cut(s) 695
PaeI GCATGC 1 cut(s) 695
PctI GAATGC 4 cut(s) 91, 211, 271, 526
PfeI GAWTC 3 cut(s) 152, 416, 1743
PinAI ACCGGT 1 cut(s) 1001
PleI GAGTC 2 cut(s) 476, 536
PluTI GGCGCC 2 cut(s) 14, 183
PpsI GAGTC 2 cut(s) 476, 536
Psp6I CCWGG 3 cut(s) 286, 460, 1375
PspEI GGTNACC 1 cut(s) 868
PspFI CCCAGC 1 cut(s) 950
PspGI CCWGG 3 cut(s) 286, 460, 1375
PspN4I GGNNCC 5 cut(s) 12, 181, 568, 802, 1692
PspPI GGNCC 1 cut(s) 1379
PstI CTGCAG 1 cut(s) 1216
PstNI CAGNNNCTG 1 cut(s) 1746
PsuI RGATCY 2 cut(s) 859, 1182
PvuII CAGCTG 1 cut(s) 1667
RsaI GTAC 4 cut(s) 887, 1325, 1414, 1692
RsaNI GTAC 4 cut(s) 886, 1324, 1413, 1691
RseI CAYNNNNRTG 1 cut(s) 1583
SaqAI TTAA 4 cut(s) 534, 1041, 1049, 1347
Sau3AI GATC 6 cut(s) 279, 370, 859, 1182, 1309, 1560
Sau96I GGNCC 1 cut(s) 1379
SchI GAGTC 2 cut(s) 476, 536
ScrFI CCNGG 3 cut(s) 288, 462, 1377
SduI GDGCHC 2 cut(s) 571, 1716
SfaNI GCATC 7 cut(s) 594, 677, 1067, 1105, 1189, 1558, 1658
SfcI CTRYAG 1 cut(s) 1212
SfoI GGCGCC 2 cut(s) 12, 181
SinI GGWCC 1 cut(s) 1379
SmiMI CAYNNNNRTG 1 cut(s) 1583
SmlI CTYRAG 1 cut(s) 1040
SmoI CTYRAG 1 cut(s) 1040
SphI GCATGC 1 cut(s) 695
Sse9I AATT 9 cut(s) 100, 549, 658, 701, 1443, 1494, 1504, 1671, 1695
SspDI GGCGCC 2 cut(s) 10, 179
SspI AATATT 2 cut(s) 997, 1160
SspMI CTAG 4 cut(s) 383, 863, 1272, 1460
StyD4I CCNGG 3 cut(s) 286, 460, 1375
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 2 cut(s) 1087, 1417
TaiI ACGT 1 cut(s) 1329
TaqI TCGA 1 cut(s) 1136
TasI AATT 9 cut(s) 100, 549, 658, 701, 1443, 1494, 1504, 1671, 1695
TatI WGTACW 1 cut(s) 1412
TauI GCSGC 5 cut(s) 16, 43, 108, 219, 311
TfiI GAWTC 3 cut(s) 152, 416, 1743
Tru1I TTAA 4 cut(s) 534, 1041, 1049, 1347
Tru9I TTAA 4 cut(s) 534, 1041, 1049, 1347
TscAI CASTG 4 cut(s) 162, 427, 793, 1415
TseFI GTSAC 4 cut(s) 160, 653, 1025, 1573
TseI GCWGC 5 cut(s) 143, 305, 1211, 1655, 1664
Tsp45I GTSAC 4 cut(s) 160, 653, 1025, 1573
TspDTI ATGAA 4 cut(s) 624, 630, 652, 1404
TspGWI ACGGA 1 cut(s) 1296
TspRI CASTG 4 cut(s) 162, 427, 793, 1415
Vha464I CTTAAG 1 cut(s) 1040
VpaK11BI GGWCC 1 cut(s) 1379
XapI RAATTY 2 cut(s) 1494, 1671
XbaI TCTAGA 1 cut(s) 1271
XceI RCATGY 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XspI CTAG 4 cut(s) 383, 863, 1272, 1460
Zsp2I ATGCAT 2 cut(s) 1060, 1651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.