Rh1DG043400

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003239
Physical Location & Seq
Reverse (-)
6902967 .. 6904644
1678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG043400.1

Sequence Viewer

Length: 552 bp
ATGCGTCTCTCTTCCTCTTTGATTGATCTAAAACCCAACCAAAATTCCTTCTCTTTTAGCTTGTTTCTGATACAGCTCAATCCTTTTCATGCGATCTTCGAAGACCCGAAGTCTTTGAAACAGGTATTAAGATATGCATCAGAAAATGTTTTCGTGCCATTAACAGTTGCAGGTAGTATTAGAGATTTTACCGATGCAACTACCAGATGTGGAGCTGAGAAGATCTCCATCGGAAGTGACGCAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGTGAAAAGTGGAAAAAGTAGCTTACAGCAGATATCTATAGTTTATGGAAATCAGGGAAAGGAATTTGATATAGATTTAGTAAAGCTGATCTCTGAGGCGGTGACCATTCCCATGCTTGCAAGTAGTGGTGCTGGTGTTGTTGAACACTTCTCAGAGGTTTTCACAAAAACAAATGCGTCTGCAGCCTTTGCTGCTGGCATTTTTCATCGATCCCAAGGAGGGATTCATTCTGTAACGGAGCACTTGCTAAAGGAAGGCATAGAAATCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

183

Amino Acids

19.9

Weight (kDa)

6.2

Isoelectric Point (pI)

25.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 113 - 163 8.1e-07 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 161
AciI CCGC 1 cut(s) 374
AclWI GGATC 1 cut(s) 480
AcsI RAATTY 2 cut(s) 43, 338
AfiI CCNNNNNNNGG 1 cut(s) 495
AgsI TTSAA 2 cut(s) 118, 419
AhdI GACNNNNNGTC 1 cut(s) 109
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AluBI AGCT 5 cut(s) 60, 76, 215, 297, 361
AluI AGCT 5 cut(s) 60, 76, 215, 297, 361
Alw21I GWGCWC 1 cut(s) 519
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 1 cut(s) 480
AlwNI CAGNNNCTG 1 cut(s) 549
ApeKI GCWGC 3 cut(s) 251, 458, 467
ApoI RAATTY 2 cut(s) 43, 338
AsuHPI GGTGA 1 cut(s) 388
AsuII TTCGAA 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 519
BbvI GCAGC 3 cut(s) 238, 454, 470
BccI CCATC 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 11
BfmI CTRYAG 3 cut(s) 252, 312, 456
BfuAI ACCTGC 1 cut(s) 161
BglII AGATCT 1 cut(s) 222
BisI GCNGC 3 cut(s) 252, 459, 468
BlsI GCNGC 3 cut(s) 253, 460, 469
BmeRI GACNNNNNGTC 1 cut(s) 109
BmsI GCATC 2 cut(s) 146, 184
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 99
Bsa29I ATCGAT 1 cut(s) 484
BsaBI GATNNNNATC 2 cut(s) 136, 227
BsaJI CCNNGG 1 cut(s) 490
Bsc4I CCNNNNNNNGG 1 cut(s) 495
Bse1I ACTGG 1 cut(s) 277
Bse8I GATNNNNATC 2 cut(s) 136, 227
BseCI ATCGAT 1 cut(s) 484
BseDI CCNNGG 1 cut(s) 490
BseJI GATNNNNATC 2 cut(s) 136, 227
BseLI CCNNNNNNNGG 1 cut(s) 495
BseMII CTCAG 3 cut(s) 207, 360, 441
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 3 cut(s) 238, 454, 470
BshVI ATCGAT 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 519
BslI CCNNNNNNNGG 1 cut(s) 495
BsmAI GTCTC 1 cut(s) 11
BsmBI CGTCTC 1 cut(s) 11
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 519
Bsp143I GATC 5 cut(s) 25, 93, 222, 363, 485
BspACI CCGC 1 cut(s) 374
BspCNI CTCAG 3 cut(s) 208, 361, 440
BspDI ATCGAT 1 cut(s) 484
BspMAI CTGCAG 2 cut(s) 256, 460
BspMI ACCTGC 1 cut(s) 161
BspPI GGATC 1 cut(s) 480
BspT104I TTCGAA 1 cut(s) 99
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 5 cut(s) 25, 93, 222, 363, 485
BssT1I CCWWGG 1 cut(s) 490
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 16
BstAPI GCANNNNNTGC 2 cut(s) 248, 464
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 393, 472
BstDEI CTNAG 3 cut(s) 216, 369, 427
BstEII GGTNACC 1 cut(s) 376
BstKTI GATC 5 cut(s) 28, 96, 225, 366, 488
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 5 cut(s) 25, 93, 222, 363, 485
BstMWI GCNNNNNNNGC 4 cut(s) 248, 458, 464, 467
BstPI GGTNACC 1 cut(s) 376
BstSFI CTRYAG 3 cut(s) 252, 312, 456
BstV1I GCAGC 3 cut(s) 238, 454, 470
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 484
BsuTUI ATCGAT 1 cut(s) 484
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 2 cut(s) 393, 472
CaiI CAGNNNCTG 1 cut(s) 549
ClaI ATCGAT 1 cut(s) 484
CseI GACGC 2 cut(s) 248, 441
CviAII CATG 2 cut(s) 89, 388
CviJI RGCY 6 cut(s) 60, 76, 215, 297, 361, 461
CviKI_1 RGCY 6 cut(s) 60, 76, 215, 297, 361, 461
DdeI CTNAG 3 cut(s) 216, 369, 427
DpnI GATC 5 cut(s) 27, 95, 224, 365, 487
DpnII GATC 5 cut(s) 25, 93, 222, 363, 485
DriI GACNNNNNGTC 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 16
Eam1105I GACNNNNNGTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 490
Eco32I GATATC 1 cut(s) 309
Eco91I GGTNACC 1 cut(s) 376
EcoO65I GGTNACC 1 cut(s) 376
EcoRV GATATC 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 490
EcoT22I ATGCAT 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 490
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 2 cut(s) 92, 391
FaiI YATR 8 cut(s) 90, 135, 249, 314, 321, 347, 389, 536
FatI CATG 2 cut(s) 88, 387
Fnu4HI GCNGC 3 cut(s) 252, 459, 468
Fsp4HI GCNGC 3 cut(s) 252, 459, 468
GluI GCNGC 3 cut(s) 252, 459, 468
GsuI CTGGAG 1 cut(s) 294
HgaI GACGC 2 cut(s) 248, 441
Hin1II CATG 2 cut(s) 92, 391
HinfI GANTC 2 cut(s) 499, 546
HphI GGTGA 1 cut(s) 388
Hpy188I TCNGA 7 cut(s) 69, 142, 233, 370, 430, 545, 551
HpyAV CCTTC 2 cut(s) 58, 524
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 6 cut(s) 137, 170, 197, 254, 395, 458
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 458, 464, 467
HpyF3I CTNAG 3 cut(s) 216, 369, 427
Hsp92II CATG 2 cut(s) 92, 391
Kzo9I GATC 5 cut(s) 25, 93, 222, 363, 485
LmnI GCTCC 2 cut(s) 212, 514
LpnPI CCDG 7 cut(s) 107, 156, 217, 258, 314, 393, 456
Lsp1109I GCAGC 3 cut(s) 238, 454, 470
LweI GCATC 2 cut(s) 146, 184
MaeIII GTNAC 3 cut(s) 236, 376, 508
MalI GATC 5 cut(s) 27, 95, 224, 365, 487
MboI GATC 5 cut(s) 25, 93, 222, 363, 485
MboII GAAGA 5 cut(s) 3, 88, 113, 232, 269
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 2 cut(s) 43, 338
MnlI CCTC 4 cut(s) 25, 364, 424, 488
Mph1103I ATGCAT 1 cut(s) 139
MseI TTAA 3 cut(s) 128, 161, 266
MslI CAYNNNNRTG 1 cut(s) 386
MwoI GCNNNNNNNGC 4 cut(s) 248, 458, 464, 467
NdeII GATC 5 cut(s) 25, 93, 222, 363, 485
NlaIII CATG 2 cut(s) 92, 391
NmuCI GTSAC 2 cut(s) 236, 376
NsiI ATGCAT 1 cut(s) 139
NspV TTCGAA 1 cut(s) 99
PfeI GAWTC 2 cut(s) 499, 546
PkrI GCNGC 3 cut(s) 253, 460, 469
PspEI GGTNACC 1 cut(s) 376
PstI CTGCAG 2 cut(s) 256, 460
PstNI CAGNNNCTG 1 cut(s) 549
PsuI RGATCY 1 cut(s) 222
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 3 cut(s) 128, 161, 266
SatI GCNGC 3 cut(s) 252, 459, 468
Sau3AI GATC 5 cut(s) 25, 93, 222, 363, 485
SduI GDGCHC 1 cut(s) 519
SetI ASST 8 cut(s) 62, 78, 126, 175, 217, 299, 363, 435
SfaNI GCATC 2 cut(s) 146, 184
SfcI CTRYAG 3 cut(s) 252, 312, 456
SfuI TTCGAA 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 386
Sse9I AATT 2 cut(s) 43, 338
SsiI CCGC 1 cut(s) 374
SspI AATATT 1 cut(s) 263
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 1 cut(s) 166
TaqI TCGA 2 cut(s) 99, 484
TasI AATT 2 cut(s) 43, 338
TfiI GAWTC 2 cut(s) 499, 546
Tru1I TTAA 3 cut(s) 128, 161, 266
Tru9I TTAA 3 cut(s) 128, 161, 266
TseFI GTSAC 2 cut(s) 236, 376
TseI GCWGC 3 cut(s) 251, 458, 467
Tsp45I GTSAC 2 cut(s) 236, 376
TspDTI ATGAA 3 cut(s) 77, 470, 491
TspGWI ACGGA 1 cut(s) 527
XapI RAATTY 2 cut(s) 43, 338
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.