Rh4DG168700

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003239
Physical Location & Seq
Forward (+)
33590492 .. 33593539
3048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG168700.1

Sequence Viewer

Length: 663 bp
ATGGCATCTCTCCCAGTGGATGTGGAGGACGATAGCTCGTTGAAACAGGTCAGCTTTCTGCATATTAACGGTTTCTGGGACTCCCCTTTGGGTGCCTTGCCTATGCTACAGGTATTAAGATATACATCAGAAAATGTTTTTGTGCCATTAACAGTTGCAGGTAGCATTAGAGATTTTACCGATGCAACTAGCAGAACTGGAGTGAAAAGTGGAAAAAGTAGCTTCAAGCAGATATCTAGAGTTTATGGAAATCAGGCTGTGGTTGTAAGCATTGATCCTTGCAAAGTGTACATTAAAAATCAAAATGATGTTGAGTTTGAGACTATAAGGGTAACAAACCCAGTCTTGAATTTTGTCGTTAAGGTTAGCGGTGGGCGGGAAAGCCAACGAATCGGAGCTTATGAGCTTACAAAAGCAGTTGAGGAGCTTGGTGCTGGAGAAATTCTGCTAAACTGCATTGATTATGAAGGTAAAGTCAGGGAAAGGGGATTTGATATAGATTTAGTAGAGCTGATCTCTGATTTGGTGACCATTCCCATGCTTGCAAGTAGTGGTGCTGGTGTTGTTGAACACTTCTTAGAGGTTCTCACGAAAGCAAATGCATCTGCTGCCTTTGCTGCTGGCATTTTTCATTCCAAGGAGGTAATACAGTCTTCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

220

Amino Acids

23.88

Weight (kDa)

5.03

Isoelectric Point (pI)

23.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 29 - 215 2.9e-22 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 92
AciI CCGC 2 cut(s) 369, 376
AclWI GGATC 1 cut(s) 269
AcsI RAATTY 2 cut(s) 349, 441
AfaI GTAC 1 cut(s) 290
AgsI TTSAA 4 cut(s) 43, 226, 349, 569
AluBI AGCT 7 cut(s) 36, 54, 222, 398, 406, 427, 511
AluI AGCT 7 cut(s) 36, 54, 222, 398, 406, 427, 511
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 1 cut(s) 269
ApeKI GCWGC 2 cut(s) 608, 617
ApoI RAATTY 2 cut(s) 349, 441
AsuHPI GGTGA 1 cut(s) 538
BanI GGYRCC 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 645
BbvI GCAGC 2 cut(s) 595, 604
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 2 cut(s) 189, 237
BfmI CTRYAG 1 cut(s) 107
BfuAI ACCTGC 1 cut(s) 149
BisI GCNGC 2 cut(s) 609, 618
BlsI GCNGC 2 cut(s) 610, 619
BmiI GGNNCC 1 cut(s) 94
BmrI ACTGGG 2 cut(s) 8, 335
BmsI GCATC 3 cut(s) 14, 172, 611
BmuI ACTGGG 2 cut(s) 8, 335
BpiI GAAGAC 1 cut(s) 645
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpmI CTGGAG 2 cut(s) 219, 456
BsaBI GATNNNNATC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 636
Bse1I ACTGG 3 cut(s) 14, 202, 341
Bse8I GATNNNNATC 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 636
BseGI GGATG 1 cut(s) 25
BseJI GATNNNNATC 1 cut(s) 124
BseNI ACTGG 3 cut(s) 14, 202, 341
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 2 cut(s) 595, 604
BshNI GGYRCC 1 cut(s) 92
BslFI GGGAC 1 cut(s) 92
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 92
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 2 cut(s) 274, 513
BspACI CCGC 2 cut(s) 369, 376
BspLI GGNNCC 1 cut(s) 94
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 1 cut(s) 269
BspT107I GGYRCC 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 3 cut(s) 14, 202, 341
BssECI CCNNGG 1 cut(s) 636
BssMI GATC 2 cut(s) 274, 513
BssT1I CCWWGG 1 cut(s) 636
Bst4CI ACNGT 3 cut(s) 71, 154, 651
BstAPI GCANNNNNTGC 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 543, 622
BstDEI CTNAG 1 cut(s) 577
BstEII GGTNACC 1 cut(s) 526
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 2 cut(s) 277, 516
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 2 cut(s) 274, 513
BstMWI GCNNNNNNNGC 3 cut(s) 608, 614, 617
BstPI GGTNACC 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 595, 604
BstV2I GAAGAC 1 cut(s) 645
BtsCI GGATG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 21
BveI ACCTGC 1 cut(s) 149
Cac8I GCNNGC 2 cut(s) 543, 622
Csp6I GTAC 1 cut(s) 289
CviAII CATG 2 cut(s) 538, 660
CviJI RGCY 9 cut(s) 36, 54, 222, 257, 384, 398, 406, 427, 511
CviKI_1 RGCY 9 cut(s) 36, 54, 222, 257, 384, 398, 406, 427, 511
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 577
DpnI GATC 2 cut(s) 276, 515
DpnII GATC 2 cut(s) 274, 513
Eco130I CCWWGG 1 cut(s) 636
Eco32I GATATC 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 526
EcoO65I GGTNACC 1 cut(s) 526
EcoRV GATATC 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 636
EcoT22I ATGCAT 1 cut(s) 604
ErhI CCWWGG 1 cut(s) 636
FaeI CATG 2 cut(s) 541, 663
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 537, 659
FauI CCCGC 1 cut(s) 369
Fnu4HI GCNGC 2 cut(s) 609, 618
FokI GGATG 1 cut(s) 32
Fsp4HI GCNGC 2 cut(s) 609, 618
FspBI CTAG 2 cut(s) 189, 237
GluI GCNGC 2 cut(s) 609, 618
GsuI CTGGAG 2 cut(s) 219, 456
Hin1II CATG 2 cut(s) 541, 663
HinfI GANTC 2 cut(s) 80, 390
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 130, 395, 520
Hpy188III TCNNGA 3 cut(s) 237, 346, 589
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 1 cut(s) 461
HpyCH4III ACNGT 3 cut(s) 71, 154, 651
HpyCH4V TGCA 7 cut(s) 61, 158, 185, 282, 456, 545, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 614, 617
HpyF3I CTNAG 1 cut(s) 577
Hsp92II CATG 2 cut(s) 541, 663
Kzo9I GATC 2 cut(s) 274, 513
LmnI GCTCC 2 cut(s) 395, 424
Lsp1109I GCAGC 2 cut(s) 595, 604
LweI GCATC 3 cut(s) 14, 172, 611
MaeI CTAG 2 cut(s) 189, 237
MaeIII GTNAC 2 cut(s) 331, 526
MalI GATC 2 cut(s) 276, 515
MboI GATC 2 cut(s) 274, 513
MboII GAAGA 1 cut(s) 645
MluCI AATT 2 cut(s) 349, 441
MlyI GAGTC 1 cut(s) 74
MnlI CCTC 4 cut(s) 19, 415, 574, 634
Mph1103I ATGCAT 1 cut(s) 604
MseI TTAA 5 cut(s) 66, 116, 149, 294, 360
MslI CAYNNNNRTG 1 cut(s) 536
MwoI GCNNNNNNNGC 3 cut(s) 608, 614, 617
NdeII GATC 2 cut(s) 274, 513
NlaIII CATG 2 cut(s) 541, 663
NlaIV GGNNCC 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 526
NsiI ATGCAT 1 cut(s) 604
PfeI GAWTC 1 cut(s) 390
PkrI GCNGC 2 cut(s) 610, 619
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PspEI GGTNACC 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 94
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 536
SaqAI TTAA 5 cut(s) 66, 116, 149, 294, 360
SatI GCNGC 2 cut(s) 609, 618
Sau3AI GATC 2 cut(s) 274, 513
SchI GAGTC 1 cut(s) 74
SfaNI GCATC 3 cut(s) 14, 172, 611
SfcI CTRYAG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 536
Sse9I AATT 2 cut(s) 349, 441
SsiI CCGC 2 cut(s) 369, 376
SspMI CTAG 2 cut(s) 189, 237
StyI CCWWGG 1 cut(s) 636
TaaI ACNGT 3 cut(s) 71, 154, 651
TasI AATT 2 cut(s) 349, 441
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 1 cut(s) 390
Tru1I TTAA 5 cut(s) 66, 116, 149, 294, 360
Tru9I TTAA 5 cut(s) 66, 116, 149, 294, 360
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 2 cut(s) 608, 617
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 2 cut(s) 480, 620
TspRI CASTG 1 cut(s) 21
XapI RAATTY 2 cut(s) 349, 441
XbaI TCTAGA 1 cut(s) 236
XspI CTAG 2 cut(s) 189, 237
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.