Rw0G015610

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00720
OG0003239
Physical Location & Seq
Forward (+)
6054 .. 12521
6468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015610.1

Sequence Viewer

Length: 741 bp
ATGCGTCTCTCTTCCTCTTTGATTGATCTAAAACCCAACCAAAATTCCTTCTCTTTTAGCTTGTTTCTGATACAGCTCAATCCTTTTCATGCGATCTTCGAAGACCCGAAGTCTGTGAAACAGGTCAGCTTTCTGCATATTAACGGTTTCTGGGACTCCCCTTTGGGTGCCTTGCCTATGCTACAGGTATTAAGATATACATCAGAAAATGTTTTTGTGCCATTAACAGTTGCAGGTAGCATTAGAGATTTTACCGATGCAACTAGCAGATGTGGGGCTGATAAGATCTCCATCGGAAGTGACACAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGTGAAAAGTGGAAAAAGTAGCTTAGAGCAGATATCTAGAGTTTATGGAAATCAGGCTGTGGTTGTAGGCATAGATCCTTGCAAAGTGTCCGTTAAAAATCAAAATGAAGTTGAGTTCAAGACTATAAGGGTTAGCGGTGGGCAGGAAGGCCGACCAATCGGAGCTTATGAGCTCGCAAAAGCAGTTGAGGAGCTTGGTGGTGGAGAAATTCTGCTAAACTGCATCTATTATGAAGGTCAGGGAAAGGGATTTGATAGAGATTTAGTGAAGCTGATCTCTGATGCGGTGACCATTCCCCTGCTTGCAAGTAGCGGTGCTGGTGTTGTTGAACACTTCTCAGATGTTTTCACGAAAACAAATGCATCTGCTGCCTTTGCTGCTGGCATTTTTCATCCCAAGGAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

247

Amino Acids

26.73

Weight (kDa)

5.65

Isoelectric Point (pI)

20.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 55 - 246 6.1e-27 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 167
AciI CCGC 3 cut(s) 474, 623, 651
AclWI GGATC 1 cut(s) 407
AcsI RAATTY 2 cut(s) 43, 546
AgsI TTSAA 2 cut(s) 457, 668
AhdI GACNNNNNGTC 1 cut(s) 109
AluBI AGCT 8 cut(s) 60, 76, 129, 360, 503, 511, 532, 610
AluI AGCT 8 cut(s) 60, 76, 129, 360, 503, 511, 532, 610
Alw21I GWGCWC 1 cut(s) 513
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 1 cut(s) 407
AoxI GGCC 1 cut(s) 487
ApeKI GCWGC 3 cut(s) 314, 707, 716
ApoI RAATTY 2 cut(s) 43, 546
AsuHPI GGTGA 1 cut(s) 637
AsuII TTCGAA 1 cut(s) 99
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 301, 694, 703
BccI CCATC 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 2 cut(s) 264, 375
BfmI CTRYAG 2 cut(s) 182, 315
BfuAI ACCTGC 1 cut(s) 224
BglII AGATCT 1 cut(s) 285
BisI GCNGC 3 cut(s) 315, 708, 717
BlsI GCNGC 3 cut(s) 316, 709, 718
BmeRI GACNNNNNGTC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 169
BmsI GCATC 4 cut(s) 247, 570, 610, 710
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 357
Bpu14I TTCGAA 1 cut(s) 99
BsaBI GATNNNNATC 2 cut(s) 199, 290
BsaJI CCNNGG 1 cut(s) 735
Bse1I ACTGG 1 cut(s) 340
Bse8I GATNNNNATC 2 cut(s) 199, 290
BseDI CCNNGG 1 cut(s) 735
BseGI GGATG 1 cut(s) 730
BseJI GATNNNNATC 2 cut(s) 199, 290
BseMII CTCAG 1 cut(s) 690
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 3 cut(s) 301, 694, 703
BshFI GGCC 1 cut(s) 489
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 513
BslFI GGGAC 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 11
BsmBI CGTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 1 cut(s) 489
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 5 cut(s) 25, 93, 285, 412, 612
BspACI CCGC 3 cut(s) 474, 623, 651
BspANI GGCC 1 cut(s) 489
BspCNI CTCAG 1 cut(s) 689
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 224
BspPI GGATC 1 cut(s) 407
BspT104I TTCGAA 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 167
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 735
BssMI GATC 5 cut(s) 25, 93, 285, 412, 612
BssT1I CCWWGG 1 cut(s) 735
Bst4CI ACNGT 3 cut(s) 146, 229, 307
Bst6I CTCTTC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 707
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 3 cut(s) 513, 642, 721
BstDEI CTNAG 2 cut(s) 361, 676
BstEII GGTNACC 1 cut(s) 625
BstF5I GGATG 1 cut(s) 730
BstKTI GATC 5 cut(s) 28, 96, 288, 415, 615
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 5 cut(s) 25, 93, 285, 412, 612
BstMWI GCNNNNNNNGC 3 cut(s) 707, 713, 716
BstPI GGTNACC 1 cut(s) 625
BstSFI CTRYAG 2 cut(s) 182, 315
BstV1I GCAGC 3 cut(s) 301, 694, 703
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 2 cut(s) 285, 412
BstYI RGATCY 2 cut(s) 285, 412
BsuRI GGCC 1 cut(s) 489
BtsCI GGATG 1 cut(s) 730
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 3 cut(s) 513, 642, 721
CviAII CATG 1 cut(s) 89
DdeI CTNAG 2 cut(s) 361, 676
DpnI GATC 5 cut(s) 27, 95, 287, 414, 614
DpnII GATC 5 cut(s) 25, 93, 285, 412, 612
DriI GACNNNNNGTC 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 16
Eam1105I GACNNNNNGTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 16
Ecl136II GAGCTC 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 513
Eco32I GATATC 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 511
Eco91I GGTNACC 1 cut(s) 625
EcoICRI GAGCTC 1 cut(s) 511
EcoO65I GGTNACC 1 cut(s) 625
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 735
EcoT22I ATGCAT 1 cut(s) 703
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 735
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 1 cut(s) 92
FaqI GGGAC 1 cut(s) 167
FatI CATG 1 cut(s) 88
Fnu4HI GCNGC 3 cut(s) 315, 708, 717
FokI GGATG 1 cut(s) 717
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 3 cut(s) 315, 708, 717
FspBI CTAG 2 cut(s) 264, 375
GluI GCNGC 3 cut(s) 315, 708, 717
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 489
Hin1II CATG 1 cut(s) 92
HinfI GANTC 1 cut(s) 155
HphI GGTGA 1 cut(s) 637
Hpy188I TCNGA 6 cut(s) 69, 205, 296, 500, 619, 679
Hpy188III TCNNGA 3 cut(s) 375, 457, 688
HpyAV CCTTC 3 cut(s) 58, 479, 566
HpyCH4III ACNGT 3 cut(s) 146, 229, 307
HpyCH4V TGCA 8 cut(s) 136, 233, 260, 317, 420, 561, 644, 701
HpyF10VI GCNNNNNNNGC 3 cut(s) 707, 713, 716
HpyF3I CTNAG 2 cut(s) 361, 676
Hsp92II CATG 1 cut(s) 92
Kzo9I GATC 5 cut(s) 25, 93, 285, 412, 612
LmnI GCTCC 2 cut(s) 500, 529
Lsp1109I GCAGC 3 cut(s) 301, 694, 703
LweI GCATC 4 cut(s) 247, 570, 610, 710
MaeI CTAG 2 cut(s) 264, 375
MaeIII GTNAC 2 cut(s) 299, 625
MalI GATC 5 cut(s) 27, 95, 287, 414, 614
MboI GATC 5 cut(s) 25, 93, 285, 412, 612
MboII GAAGA 4 cut(s) 3, 88, 113, 332
MflI RGATCY 2 cut(s) 285, 412
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 2 cut(s) 43, 546
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 2 cut(s) 25, 520
Mph1103I ATGCAT 1 cut(s) 703
MseI TTAA 5 cut(s) 141, 191, 224, 329, 432
MwoI GCNNNNNNNGC 3 cut(s) 707, 713, 716
NdeII GATC 5 cut(s) 25, 93, 285, 412, 612
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 2 cut(s) 299, 625
NsiI ATGCAT 1 cut(s) 703
NspV TTCGAA 1 cut(s) 99
PkrI GCNGC 3 cut(s) 316, 709, 718
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 513
PspEI GGTNACC 1 cut(s) 625
PspN4I GGNNCC 1 cut(s) 169
PstI CTGCAG 1 cut(s) 319
PsuI RGATCY 2 cut(s) 285, 412
SacI GAGCTC 1 cut(s) 513
SaqAI TTAA 5 cut(s) 141, 191, 224, 329, 432
SatI GCNGC 3 cut(s) 315, 708, 717
Sau3AI GATC 5 cut(s) 25, 93, 285, 412, 612
SchI GAGTC 1 cut(s) 149
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 4 cut(s) 247, 570, 610, 710
SfcI CTRYAG 2 cut(s) 182, 315
SfuI TTCGAA 1 cut(s) 99
Sse9I AATT 2 cut(s) 43, 546
SsiI CCGC 3 cut(s) 474, 623, 651
SspI AATATT 1 cut(s) 326
SspMI CTAG 2 cut(s) 264, 375
SstI GAGCTC 1 cut(s) 513
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 3 cut(s) 146, 229, 307
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 43, 546
Tru1I TTAA 5 cut(s) 141, 191, 224, 329, 432
Tru9I TTAA 5 cut(s) 141, 191, 224, 329, 432
TseFI GTSAC 2 cut(s) 299, 625
TseI GCWGC 3 cut(s) 314, 707, 716
Tsp45I GTSAC 2 cut(s) 299, 625
TspDTI ATGAA 4 cut(s) 77, 459, 585, 719
TspGWI ACGGA 1 cut(s) 418
XapI RAATTY 2 cut(s) 43, 546
XbaI TCTAGA 1 cut(s) 374
XspI CTAG 2 cut(s) 264, 375
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.