Rw1G002220

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003239
Physical Location & Seq
Forward (+)
3957442 .. 3959249
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002220.1

Sequence Viewer

Length: 528 bp
ATGCGATCTTCGAAGACCCGAAGTCTGTCAGCTAGCTTTCTGAATATTAACGGTTTCCGGGACTCCCCTTTGGGTGCCTTACCTATGCTACAGGTATTAAGATATGCATCAGAAAATGTTTTCGTGCCATTAACAGCTGCAGGTAGTATTAGAGATTTTACCGATGCAACTACCAGGCACTATTCTAGCTTGGAAGTTGCTTTACAATATTTCAGATGTGGAGCTGAGAAGATCTCCATCGGAAGTGACGCAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGTGAAAAGTGGAAAAAGTAGCTTACAGCAGATATCTATAGTTTATGGAAATCAGGGAAAGGAATTTGATATAGATTTAGTAAAGCTGATCTCTGAGGCGGTGACCATTCCCATGCTTGCAAGTAGTGGTGCTGGTGTTGTTGAACACTTCTCAGAGGTTTTCACAAAAACAAATGCGTCTGCAGCCTTTGCTGCTGGCATTTTTCATCGATCCCAAGGAGGTATCAATGTCAAATACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

175

Amino Acids

18.85

Weight (kDa)

8.69

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 116 - 166 7.8e-07 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 74
AciI CCGC 1 cut(s) 383
AclWI GGATC 1 cut(s) 489
AcsI RAATTY 1 cut(s) 347
AgsI TTSAA 1 cut(s) 428
AhdI GACNNNNNGTC 1 cut(s) 21
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 7 cut(s) 32, 36, 137, 189, 224, 306, 370
AluI AGCT 7 cut(s) 32, 36, 137, 189, 224, 306, 370
AlwI GGATC 1 cut(s) 489
ApeKI GCWGC 4 cut(s) 137, 260, 467, 476
ApoI RAATTY 1 cut(s) 347
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 397
AsuII TTCGAA 1 cut(s) 11
AsuNHI GCTAGC 1 cut(s) 32
BanI GGYRCC 1 cut(s) 74
BarI GAAGNNNNNNTAC 2 cut(s) 186, 218
BbsI GAAGAC 1 cut(s) 20
BbvI GCAGC 4 cut(s) 124, 247, 463, 479
BccI CCATC 1 cut(s) 245
BciT130I CCWGG 1 cut(s) 175
BcnI CCSGG 1 cut(s) 59
BfaI CTAG 2 cut(s) 33, 186
BfmI CTRYAG 5 cut(s) 89, 138, 261, 321, 465
BfuAI ACCTGC 1 cut(s) 131
BglII AGATCT 1 cut(s) 231
BisI GCNGC 4 cut(s) 138, 261, 468, 477
BlsI GCNGC 4 cut(s) 139, 262, 469, 478
Bme1390I CCNGG 2 cut(s) 59, 175
BmeRI GACNNNNNGTC 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 2 cut(s) 59, 175
BmsI GCATC 2 cut(s) 116, 154
BmtI GCTAGC 1 cut(s) 36
BpiI GAAGAC 1 cut(s) 20
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 59
Bsa29I ATCGAT 1 cut(s) 493
BsaBI GATNNNNATC 2 cut(s) 106, 236
BsaJI CCNNGG 1 cut(s) 499
Bse1I ACTGG 1 cut(s) 286
Bse8I GATNNNNATC 2 cut(s) 106, 236
BseBI CCWGG 1 cut(s) 175
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 1 cut(s) 499
BseJI GATNNNNATC 2 cut(s) 106, 236
BseMII CTCAG 3 cut(s) 216, 369, 450
BseNI ACTGG 1 cut(s) 286
BseXI GCAGC 4 cut(s) 124, 247, 463, 479
BshNI GGYRCC 1 cut(s) 74
BshVI ATCGAT 1 cut(s) 493
BsiSI CCGG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 11
Bsp143I GATC 4 cut(s) 5, 231, 372, 494
BspACI CCGC 1 cut(s) 383
BspCNI CTCAG 3 cut(s) 217, 370, 449
BspDI ATCGAT 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 76
BspMAI CTGCAG 3 cut(s) 142, 265, 469
BspMI ACCTGC 1 cut(s) 131
BspOI GCTAGC 1 cut(s) 36
BspPI GGATC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 74
BsrI ACTGG 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 4 cut(s) 5, 231, 372, 494
BssT1I CCWWGG 1 cut(s) 499
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 2 cut(s) 53, 524
BstAPI GCANNNNNTGC 2 cut(s) 257, 473
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 34, 402, 481
BstDEI CTNAG 3 cut(s) 225, 378, 436
BstEII GGTNACC 1 cut(s) 385
BstKTI GATC 4 cut(s) 8, 234, 375, 497
BstMBI GATC 4 cut(s) 5, 231, 372, 494
BstMWI GCNNNNNNNGC 4 cut(s) 257, 467, 473, 476
BstNI CCWGG 1 cut(s) 175
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 57, 173
BstSFI CTRYAG 5 cut(s) 89, 138, 261, 321, 465
BstV1I GCAGC 4 cut(s) 124, 247, 463, 479
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 493
BsuTUI ATCGAT 1 cut(s) 493
BveI ACCTGC 1 cut(s) 131
Cac8I GCNNGC 3 cut(s) 34, 402, 481
ClaI ATCGAT 1 cut(s) 493
CseI GACGC 2 cut(s) 257, 450
CviAII CATG 1 cut(s) 397
CviJI RGCY 8 cut(s) 32, 36, 137, 189, 224, 306, 370, 470
CviKI_1 RGCY 8 cut(s) 32, 36, 137, 189, 224, 306, 370, 470
DdeI CTNAG 3 cut(s) 225, 378, 436
DpnI GATC 4 cut(s) 7, 233, 374, 496
DpnII GATC 4 cut(s) 5, 231, 372, 494
DriI GACNNNNNGTC 1 cut(s) 21
Eam1105I GACNNNNNGTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 499
Eco32I GATATC 1 cut(s) 318
Eco91I GGTNACC 1 cut(s) 385
EcoO65I GGTNACC 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 173
EcoRV GATATC 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 1 cut(s) 400
FaiI YATR 7 cut(s) 86, 105, 258, 323, 330, 356, 398
FaqI GGGAC 1 cut(s) 74
FatI CATG 1 cut(s) 396
Fnu4HI GCNGC 4 cut(s) 138, 261, 468, 477
Fsp4HI GCNGC 4 cut(s) 138, 261, 468, 477
FspBI CTAG 2 cut(s) 33, 186
GluI GCNGC 4 cut(s) 138, 261, 468, 477
GsuI CTGGAG 1 cut(s) 303
HapII CCGG 1 cut(s) 58
HgaI GACGC 2 cut(s) 257, 450
Hin1II CATG 1 cut(s) 400
HinfI GANTC 1 cut(s) 62
HpaII CCGG 1 cut(s) 58
HphI GGTGA 1 cut(s) 397
Hpy188I TCNGA 6 cut(s) 42, 112, 215, 242, 379, 439
HpyCH4III ACNGT 2 cut(s) 53, 524
HpyCH4V TGCA 6 cut(s) 107, 140, 167, 263, 404, 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 467, 473, 476
HpyF3I CTNAG 3 cut(s) 225, 378, 436
Hsp92II CATG 1 cut(s) 400
Kzo9I GATC 4 cut(s) 5, 231, 372, 494
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 9 cut(s) 71, 77, 126, 160, 187, 267, 323, 402, 465
Lsp1109I GCAGC 4 cut(s) 124, 247, 463, 479
LweI GCATC 2 cut(s) 116, 154
MaeI CTAG 2 cut(s) 33, 186
MaeIII GTNAC 2 cut(s) 245, 385
MalI GATC 4 cut(s) 7, 233, 374, 496
MboI GATC 4 cut(s) 5, 231, 372, 494
MboII GAAGA 3 cut(s) 25, 241, 278
MflI RGATCY 1 cut(s) 231
MluCI AATT 1 cut(s) 347
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 3 cut(s) 373, 433, 497
Mph1103I ATGCAT 1 cut(s) 109
MseI TTAA 4 cut(s) 48, 98, 131, 275
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 59, 175
MvaI CCWGG 1 cut(s) 175
MwoI GCNNNNNNNGC 4 cut(s) 257, 467, 473, 476
NciI CCSGG 1 cut(s) 59
NdeII GATC 4 cut(s) 5, 231, 372, 494
NheI GCTAGC 1 cut(s) 32
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 245, 385
NsiI ATGCAT 1 cut(s) 109
NspV TTCGAA 1 cut(s) 11
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 4 cut(s) 139, 262, 469, 478
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 173
PspEI GGTNACC 1 cut(s) 385
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 76
PstI CTGCAG 3 cut(s) 142, 265, 469
PsuI RGATCY 1 cut(s) 231
PvuII CAGCTG 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 4 cut(s) 48, 98, 131, 275
SatI GCNGC 4 cut(s) 138, 261, 468, 477
Sau3AI GATC 4 cut(s) 5, 231, 372, 494
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 2 cut(s) 59, 175
SfaNI GCATC 2 cut(s) 116, 154
SfcI CTRYAG 5 cut(s) 89, 138, 261, 321, 465
SfuI TTCGAA 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 1 cut(s) 347
SsiI CCGC 1 cut(s) 383
SspI AATATT 3 cut(s) 46, 209, 272
SspMI CTAG 2 cut(s) 33, 186
StyD4I CCNGG 2 cut(s) 57, 173
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 2 cut(s) 53, 524
TaqI TCGA 2 cut(s) 11, 493
TasI AATT 1 cut(s) 347
Tru1I TTAA 4 cut(s) 48, 98, 131, 275
Tru9I TTAA 4 cut(s) 48, 98, 131, 275
TseFI GTSAC 2 cut(s) 245, 385
TseI GCWGC 4 cut(s) 137, 260, 467, 476
Tsp45I GTSAC 2 cut(s) 245, 385
TspDTI ATGAA 1 cut(s) 479
XapI RAATTY 1 cut(s) 347
XspI CTAG 2 cut(s) 33, 186
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.