Rh5BG277100

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003239
Physical Location & Seq
Reverse (-)
34921011 .. 34928662
7652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG277100.1

Sequence Viewer

Length: 447 bp
ATGTTTGTTCTTAAGGTTAGTGGTGGCCGGGAAGGCAGACCAATTGGAGCTTATGAGCTTACAAAAGCAGTTGAGGAGCTTGGAGCTAGAGAAATTCTTCTAAACTACATTGATTGCTATGTAAAGCTCATCTCTGATGCTGTGACCATTCCCGTGATTGCAAGTAGTGGTTCTGGTGTTGTTAAACACTTCTCAGAGGTTTTCACGAAAACAAATGCATCTGCTGCCCTTGCTGTTCGCATATTCCATCGCAAGGAGAGCACCAATTATTTCCCTTCTAATAATAGTGATACACGAAATCCTTATCTTAAGCAAATGGTTGGAAATTTTGCAAACTCGTGGGATAAGATGACAAGGAAAAGCGCCAATCTTCTAAGCTCTTTTTACAACAAGAAATGGAAAGATGAGGCTTGTACCTTTCGTGCTGAAGAGTACTTTTCGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

148

Amino Acids

16.66

Weight (kDa)

9.13

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 5 - 86 7.2e-09 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 2 cut(s) 93, 325
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 2 cut(s) 415, 434
AfiI CCNNNNNNNGG 1 cut(s) 253
AflII CTTAAG 2 cut(s) 11, 308
AluBI AGCT 6 cut(s) 50, 58, 79, 86, 127, 378
AluI AGCT 6 cut(s) 50, 58, 79, 86, 127, 378
Alw21I GWGCWC 1 cut(s) 263
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 2 cut(s) 93, 325
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 365
AsuC2I CCSGG 1 cut(s) 29
BauI CACGAG 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 211
BccI CCATC 1 cut(s) 255
BcnI CCSGG 1 cut(s) 29
BfaI CTAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 366
BfrI CTTAAG 2 cut(s) 11, 308
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 2 cut(s) 127, 227
BpuMI CCSGG 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 253
BseLI CCNNNNNNNGG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 1 cut(s) 211
BshFI GGCC 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 263
BsiSI CCGG 1 cut(s) 28
BslI CCNNNNNNNGG 1 cut(s) 253
BsnI GGCC 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 263
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 1 cut(s) 206
BspTI CTTAAG 2 cut(s) 11, 308
BssSI CACGAG 1 cut(s) 337
Bst2BI CACGAG 1 cut(s) 337
Bst6I CTCTTC 1 cut(s) 423
BstAFI CTTAAG 2 cut(s) 11, 308
BstAPI GCANNNNNTGC 1 cut(s) 224
BstDEI CTNAG 2 cut(s) 193, 374
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 1 cut(s) 365
BstMWI GCNNNNNNNGC 4 cut(s) 33, 224, 230, 258
BstSCI CCNGG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 233
CfoI GCGC 1 cut(s) 365
Csp6I GTAC 2 cut(s) 414, 433
CviJI RGCY 8 cut(s) 27, 50, 58, 79, 86, 127, 378, 410
CviKI_1 RGCY 8 cut(s) 27, 50, 58, 79, 86, 127, 378, 410
CviQI GTAC 2 cut(s) 414, 433
DdeI CTNAG 2 cut(s) 193, 374
EaeI YGGCCR 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 220
FaiI YATR 3 cut(s) 54, 120, 242
Fnu4HI GCNGC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 364
GluI GCNGC 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 1 cut(s) 27
HapII CCGG 1 cut(s) 28
HhaI GCGC 1 cut(s) 365
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HpaII CCGG 1 cut(s) 28
Hpy188I TCNGA 2 cut(s) 136, 196
Hpy188III TCNNGA 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 26, 285
HpyCH4V TGCA 3 cut(s) 161, 218, 332
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 224, 230, 258
HpyF3I CTNAG 2 cut(s) 193, 374
HspAI GCGC 1 cut(s) 363
LmnI GCTCC 3 cut(s) 47, 76, 83
LpnPI CCDG 2 cut(s) 41, 159
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 127, 227
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 142
MboII GAAGA 3 cut(s) 89, 362, 440
MfeI CAATTG 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 263
MluCI AATT 4 cut(s) 42, 93, 265, 325
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 3 cut(s) 67, 190, 400
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 3 cut(s) 12, 183, 309
MslI CAYNNNNRTG 1 cut(s) 152
MspCI CTTAAG 2 cut(s) 11, 308
MspI CCGG 1 cut(s) 28
MspR9I CCNGG 1 cut(s) 29
MunI CAATTG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 33, 224, 230, 258
NciI CCSGG 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 96
PkrI GCNGC 1 cut(s) 226
RsaI GTAC 2 cut(s) 415, 434
RsaNI GTAC 2 cut(s) 414, 433
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 3 cut(s) 12, 183, 309
SatI GCNGC 1 cut(s) 225
ScaI AGTACT 1 cut(s) 434
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 1 cut(s) 263
SetI ASST 9 cut(s) 18, 52, 60, 81, 88, 129, 201, 380, 419
SfaNI GCATC 2 cut(s) 127, 227
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 11, 308
SmoI CTYRAG 2 cut(s) 11, 308
Sse9I AATT 4 cut(s) 42, 93, 265, 325
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 27
TasI AATT 4 cut(s) 42, 93, 265, 325
TatI WGTACW 1 cut(s) 432
Tru1I TTAA 3 cut(s) 12, 183, 309
Tru9I TTAA 3 cut(s) 12, 183, 309
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 142
Vha464I CTTAAG 2 cut(s) 11, 308
XapI RAATTY 2 cut(s) 93, 325
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 1 cut(s) 87
ZrmI AGTACT 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.