Rh4CG184200

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003239
Physical Location & Seq
Forward (+)
39724485 .. 39734702
10218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG184200.1

Sequence Viewer

Length: 765 bp
ATGAAGTCATTGCCCACATATAAGTCGTTGAAACAGGTCAGCTTTCTGCATATTAACGGTTTCTGGGACTCCCCTTTGGGTGCCTTGCCTATGCTACAGGTATTAAGATATACATCAGAAAATGTTTTTGTGCCATTAACAGTTGCAGGTAGCATTAGAGATTTTACCAATGTAACTAGCAGATGTGGGGCTGATAAGATCTCCATCGGAAGTGACACAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGTGAAAAGTGGAAAAAGTAGCTTCGAGCAGATATCTAGAGTTTATGGAAATCAGGCTGTGGTTGTAAGCATTGATCCTTGCAAAGTGTACGTTAAAAATCAAAATGATGTTGAGTTCGAGACTATAAGGGTAACAAACCCAGTCTTGAATTTTGTCCTTAAGGTTAGCGGTGGGTGGGAAAGCCAACGAATCGGAGCTTATGAGCTTACAAAAGCAGTTGAGGAGCTTGGTGCTGGAGAAATTCTGCTAAACTGCATTGATTATGAAAGTAAAGTTCAGTTTTTGCGCTTCTTATTCTTGCTTATGAAAACCACTTATATGAGAATTGCAAAGAGAAATTTGTCTATACATTTAATCAATGGCATGTTAGTTTATCACTCATCACGGTCTGAGATAATGCCAAAATGTTGGAAGTTATCTGGATGTGTTTGGGAAGGCATTGCTATCCTTTATTGTGTTCTCCATTCCTTATTCAAACTAGTGGTTTGTGCTAGCTATATATTAACTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

254

Amino Acids

28.55

Weight (kDa)

8.88

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 26 - 172 4.3e-10 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 80
AciI CCGC 1 cut(s) 420
AclWI GGATC 1 cut(s) 320
AcsI RAATTY 3 cut(s) 400, 492, 589
AfaI GTAC 1 cut(s) 341
AflII CTTAAG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 31, 400, 727
AhlI ACTAGT 1 cut(s) 730
AluBI AGCT 6 cut(s) 42, 273, 449, 457, 478, 747
AluI AGCT 6 cut(s) 42, 273, 449, 457, 478, 747
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 1 cut(s) 320
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 3 cut(s) 400, 492, 589
AspLEI GCGC 1 cut(s) 540
AsuNHI GCTAGC 1 cut(s) 743
BanI GGYRCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 365
BcuI ACTAGT 1 cut(s) 730
BfaI CTAG 4 cut(s) 177, 288, 731, 744
BfmI CTRYAG 2 cut(s) 95, 228
BfrI CTTAAG 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 137
BglII AGATCT 1 cut(s) 198
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
BmiI GGNNCC 1 cut(s) 82
BmrI ACTGGG 1 cut(s) 386
BmtI GCTAGC 1 cut(s) 747
BmuI ACTGGG 1 cut(s) 386
BpmI CTGGAG 2 cut(s) 270, 507
BsaBI GATNNNNATC 2 cut(s) 112, 203
Bse1I ACTGG 2 cut(s) 253, 392
Bse3DI GCAATG 2 cut(s) 8, 690
Bse8I GATNNNNATC 2 cut(s) 112, 203
BseGI GGATG 1 cut(s) 680
BseJI GATNNNNATC 2 cut(s) 112, 203
BseMI GCAATG 2 cut(s) 8, 690
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 2 cut(s) 253, 392
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 1 cut(s) 214
BshNI GGYRCC 1 cut(s) 80
BslFI GGGAC 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 80
Bsp143I GATC 2 cut(s) 198, 325
BspACI CCGC 1 cut(s) 420
BspCNI CTCAG 1 cut(s) 634
BspLI GGNNCC 1 cut(s) 82
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 137
BspOI GCTAGC 1 cut(s) 747
BspPI GGATC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 80
BspTI CTTAAG 1 cut(s) 410
BsrDI GCAATG 2 cut(s) 8, 690
BsrI ACTGG 2 cut(s) 253, 392
BssMI GATC 2 cut(s) 198, 325
Bst4CI ACNGT 5 cut(s) 59, 142, 220, 639, 760
BstAFI CTTAAG 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 745
BstDEI CTNAG 1 cut(s) 642
BstF5I GGATG 1 cut(s) 680
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 2 cut(s) 201, 328
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 2 cut(s) 198, 325
BstNSI RCATGY 1 cut(s) 619
BstSFI CTRYAG 2 cut(s) 95, 228
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 198
BstXI CCANNNNNNTGG 1 cut(s) 660
BstYI RGATCY 1 cut(s) 198
BtsCI GGATG 1 cut(s) 680
BveI ACCTGC 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 745
CfoI GCGC 1 cut(s) 540
Csp6I GTAC 1 cut(s) 340
CviAII CATG 1 cut(s) 616
CviJI RGCY 9 cut(s) 42, 191, 273, 308, 435, 449, 457, 478, 747
CviKI_1 RGCY 9 cut(s) 42, 191, 273, 308, 435, 449, 457, 478, 747
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 642
DpnI GATC 2 cut(s) 200, 327
DpnII GATC 2 cut(s) 198, 325
Eco32I GATATC 1 cut(s) 285
EcoRV GATATC 1 cut(s) 285
FaeI CATG 1 cut(s) 619
FaqI GGGAC 1 cut(s) 80
FatI CATG 1 cut(s) 615
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 1 cut(s) 687
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 4 cut(s) 177, 288, 731, 744
GlaI GCGC 1 cut(s) 539
GluI GCNGC 1 cut(s) 228
GsuI CTGGAG 2 cut(s) 270, 507
HhaI GCGC 1 cut(s) 540
Hin1II CATG 1 cut(s) 619
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HinfI GANTC 2 cut(s) 68, 441
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 4 cut(s) 118, 209, 446, 643
Hpy188III TCNNGA 4 cut(s) 288, 370, 397, 672
Hpy8I GTNNAC 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 680
HpyCH4III ACNGT 5 cut(s) 59, 142, 220, 639, 760
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 6 cut(s) 49, 146, 230, 333, 507, 581
HpyF3I CTNAG 1 cut(s) 642
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 1 cut(s) 619
HspAI GCGC 1 cut(s) 538
Kzo9I GATC 2 cut(s) 198, 325
LmnI GCTCC 2 cut(s) 446, 475
LpnPI CCDG 9 cut(s) 20, 49, 83, 132, 234, 290, 405, 471, 657
Lsp1109I GCAGC 1 cut(s) 214
MaeI CTAG 4 cut(s) 177, 288, 731, 744
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 3 cut(s) 172, 212, 382
MalI GATC 2 cut(s) 200, 327
MboI GATC 2 cut(s) 198, 325
MboII GAAGA 1 cut(s) 245
MflI RGATCY 1 cut(s) 198
MluCI AATT 4 cut(s) 400, 492, 576, 589
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 641
MnlI CCTC 1 cut(s) 466
MseI TTAA 8 cut(s) 54, 104, 137, 242, 345, 411, 605, 755
MslI CAYNNNNRTG 1 cut(s) 569
MspCI CTTAAG 1 cut(s) 410
NdeII GATC 2 cut(s) 198, 325
NheI GCTAGC 1 cut(s) 743
NlaIII CATG 1 cut(s) 619
NlaIV GGNNCC 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 212
NspI RCATGY 1 cut(s) 619
PfeI GAWTC 1 cut(s) 441
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PspN4I GGNNCC 1 cut(s) 82
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 198
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 569
SaqAI TTAA 8 cut(s) 54, 104, 137, 242, 345, 411, 605, 755
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 2 cut(s) 198, 325
SchI GAGTC 1 cut(s) 62
SfcI CTRYAG 2 cut(s) 95, 228
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
SpeI ACTAGT 1 cut(s) 730
Sse9I AATT 4 cut(s) 400, 492, 576, 589
SsiI CCGC 1 cut(s) 420
SspI AATATT 1 cut(s) 239
SspMI CTAG 4 cut(s) 177, 288, 731, 744
TaaI ACNGT 5 cut(s) 59, 142, 220, 639, 760
TaiI ACGT 1 cut(s) 345
TaqI TCGA 2 cut(s) 276, 369
TasI AATT 4 cut(s) 400, 492, 576, 589
TfiI GAWTC 1 cut(s) 441
Tru1I TTAA 8 cut(s) 54, 104, 137, 242, 345, 411, 605, 755
Tru9I TTAA 8 cut(s) 54, 104, 137, 242, 345, 411, 605, 755
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 3 cut(s) 17, 531, 572
Vha464I CTTAAG 1 cut(s) 410
XapI RAATTY 3 cut(s) 400, 492, 589
XbaI TCTAGA 1 cut(s) 287
XceI RCATGY 1 cut(s) 619
XspI CTAG 4 cut(s) 177, 288, 731, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.