Rh4AG173300

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003239
Physical Location & Seq
Forward (+)
43474449 .. 43498812
24364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG173300.1

Sequence Viewer

Length: 732 bp
ATGGCAATCGGGATTAAGGCGCGGATCCAGAGCGGCATTGGAGGAGTCGTTTTTGCTTGTCTATGGTTTTATTTTAGGTGCTTGTTAAAAGCTTATTTGATTCTTATCCTCAACGAAATGACGATACCTTACGTAATAGACACTCAATCTATAGCTTCCACTCAAAATTTATATGACCTATCAAATGTAGCATGGCACTATTCTAGCAATATTTCAGATGTGAAAAGTGGAAAAAGTATCTTCGAGCAGATATCTAGAGTTTATGGAAATCAGGCTGTGGTTGTAAGCATTGATCCTTGCAAAGTGTACGTTAAAAATCAAAATGATGTTGAGTTCGAGACTATAAGGGTAACAAACCCAGTCTTGAATTTTGTCCTTAAGGTTAGCGGTGGGCGGGAAAGCCAACGAATCGGAGCTTATGAGCTTACAAAAGCAGTTGAGGAGCTTGGTGCTGGAGAAATTCTGCTAAACTGCATTGATTATGAAGGTAAAGTTCAGTTTTTGCGCTCCTTATTCTTGCTTATGAAAACCACTTATATGAGAATTGCAAAGAGAAATTTGTCTATACATTTAATCAATGGCATGTTAGTTTATCACTCATCACGGTCTGAGATAATGCCAAAATGTTGGAAGTCATCTAGATGTGTTTGGGAAGGCATTGCTATCCTTTATTGTGTTCTCCATTCCTTATTCAAACTAGTGGTTTGTGCTAGTTATATATTAACTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

243

Amino Acids

27.49

Weight (kDa)

8.95

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 78 - 165 3.2e-07 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 33
AccII CGCG 1 cut(s) 22
AciI CCGC 4 cut(s) 22, 33, 387, 394
AclWI GGATC 3 cut(s) 19, 32, 287
AcsI RAATTY 4 cut(s) 166, 367, 459, 556
AfaI GTAC 1 cut(s) 308
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 367, 694
AhlI ACTAGT 1 cut(s) 697
AluBI AGCT 5 cut(s) 92, 155, 416, 424, 445
AluI AGCT 5 cut(s) 92, 155, 416, 424, 445
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 3 cut(s) 19, 32, 287
ApoI RAATTY 4 cut(s) 166, 367, 459, 556
AspLEI GCGC 2 cut(s) 22, 507
BamHI GGATCC 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 332
BcuI ACTAGT 1 cut(s) 697
BfaI CTAG 5 cut(s) 204, 255, 639, 698, 711
BfmI CTRYAG 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 377
BisI GCNGC 1 cut(s) 34
BlsI GCNGC 1 cut(s) 35
BmiI GGNNCC 1 cut(s) 26
BmrI ACTGGG 1 cut(s) 353
BmuI ACTGGG 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 474
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 104
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bse1I ACTGG 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 657
Bse8I GATNNNNATC 1 cut(s) 104
BseJI GATNNNNATC 1 cut(s) 104
BseMI GCAATG 1 cut(s) 657
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 2 cut(s) 57, 455
Bsh1236I CGCG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 332
Bsp143I GATC 2 cut(s) 24, 292
BspACI CCGC 4 cut(s) 22, 33, 387, 394
BspCNI CTCAG 1 cut(s) 601
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 26
BspPI GGATC 3 cut(s) 19, 32, 287
BspTI CTTAAG 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 657
BsrI ACTGG 1 cut(s) 359
BssMI GATC 2 cut(s) 24, 292
Bst4CI ACNGT 2 cut(s) 606, 727
BstAFI CTTAAG 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 133
BstDEI CTNAG 1 cut(s) 609
BstFNI CGCG 1 cut(s) 22
BstHHI GCGC 2 cut(s) 22, 507
BstKTI GATC 2 cut(s) 27, 295
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 2 cut(s) 24, 292
BstNSI RCATGY 1 cut(s) 586
BstSFI CTRYAG 1 cut(s) 150
BstSNI TACGTA 1 cut(s) 133
BstUI CGCG 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 24
BstXI CCANNNNNNTGG 1 cut(s) 627
BstYI RGATCY 1 cut(s) 24
CfoI GCGC 2 cut(s) 22, 507
Csp6I GTAC 1 cut(s) 307
CviAII CATG 2 cut(s) 192, 583
CviJI RGCY 7 cut(s) 92, 155, 275, 402, 416, 424, 445
CviKI_1 RGCY 7 cut(s) 92, 155, 275, 402, 416, 424, 445
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 1 cut(s) 609
DpnI GATC 2 cut(s) 26, 294
DpnII GATC 2 cut(s) 24, 292
Eco105I TACGTA 1 cut(s) 133
Eco32I GATATC 1 cut(s) 252
EcoRV GATATC 1 cut(s) 252
FaeI CATG 2 cut(s) 195, 586
FatI CATG 2 cut(s) 191, 582
FauI CCCGC 1 cut(s) 387
Fnu4HI GCNGC 1 cut(s) 34
Fsp4HI GCNGC 1 cut(s) 34
FspBI CTAG 5 cut(s) 204, 255, 639, 698, 711
GlaI GCGC 2 cut(s) 21, 506
GluI GCNGC 1 cut(s) 34
GsuI CTGGAG 1 cut(s) 474
HhaI GCGC 2 cut(s) 22, 507
Hin1II CATG 2 cut(s) 195, 586
Hin6I GCGC 2 cut(s) 20, 505
HinP1I GCGC 2 cut(s) 20, 505
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 3 cut(s) 45, 100, 408
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 3 cut(s) 217, 413, 610
Hpy188III TCNNGA 6 cut(s) 10, 28, 255, 337, 364, 639
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 479, 647
HpyCH4III ACNGT 2 cut(s) 606, 727
HpyCH4IV ACGT 2 cut(s) 132, 309
HpyCH4V TGCA 3 cut(s) 300, 474, 548
HpyF3I CTNAG 1 cut(s) 609
HpySE526I ACGT 2 cut(s) 132, 309
Hsp92II CATG 2 cut(s) 195, 586
HspAI GCGC 2 cut(s) 20, 505
Kzo9I GATC 2 cut(s) 24, 292
LmnI GCTCC 3 cut(s) 413, 442, 512
LpnPI CCDG 4 cut(s) 41, 257, 372, 438
MaeI CTAG 5 cut(s) 204, 255, 639, 698, 711
MaeII ACGT 2 cut(s) 132, 309
MaeIII GTNAC 1 cut(s) 349
MalI GATC 2 cut(s) 26, 294
MbiI CCGCTC 1 cut(s) 33
MboI GATC 2 cut(s) 24, 292
MboII GAAGA 1 cut(s) 232
MflI RGATCY 1 cut(s) 24
MluCI AATT 5 cut(s) 166, 367, 459, 543, 556
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 608
MnlI CCTC 3 cut(s) 35, 119, 433
MseI TTAA 6 cut(s) 15, 86, 312, 378, 572, 722
MslI CAYNNNNRTG 2 cut(s) 536, 640
MspCI CTTAAG 1 cut(s) 377
MvnI CGCG 1 cut(s) 22
NdeII GATC 2 cut(s) 24, 292
NlaIII CATG 2 cut(s) 195, 586
NlaIV GGNNCC 1 cut(s) 26
NspI RCATGY 1 cut(s) 586
PfeI GAWTC 2 cut(s) 100, 408
PkrI GCNGC 1 cut(s) 35
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Ppu21I YACGTR 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 26
PsuI RGATCY 1 cut(s) 24
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 536, 640
SaqAI TTAA 6 cut(s) 15, 86, 312, 378, 572, 722
SatI GCNGC 1 cut(s) 34
Sau3AI GATC 2 cut(s) 24, 292
SchI GAGTC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 150
SmiMI CAYNNNNRTG 2 cut(s) 536, 640
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SnaBI TACGTA 1 cut(s) 133
SpeI ACTAGT 1 cut(s) 697
Sse9I AATT 5 cut(s) 166, 367, 459, 543, 556
SsiI CCGC 4 cut(s) 22, 33, 387, 394
SspI AATATT 1 cut(s) 211
SspMI CTAG 5 cut(s) 204, 255, 639, 698, 711
TaaI ACNGT 2 cut(s) 606, 727
TaiI ACGT 2 cut(s) 135, 312
TaqI TCGA 2 cut(s) 243, 336
TasI AATT 5 cut(s) 166, 367, 459, 543, 556
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 2 cut(s) 100, 408
Tru1I TTAA 6 cut(s) 15, 86, 312, 378, 572, 722
Tru9I TTAA 6 cut(s) 15, 86, 312, 378, 572, 722
TspDTI ATGAA 2 cut(s) 498, 539
Vha464I CTTAAG 1 cut(s) 377
XapI RAATTY 4 cut(s) 166, 367, 459, 556
XbaI TCTAGA 2 cut(s) 254, 638
XceI RCATGY 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XspI CTAG 5 cut(s) 204, 255, 639, 698, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.