Rroxscaffold_1G00040000

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003239
Physical Location & Seq
Forward (+)
57648045 .. 57654695
6651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040000.1

Sequence Viewer

Length: 1764 bp
ATGGAGGCGCCGCCAATTGTTTCCCCTACCAAAACGCTGCCGTTCCGATCAGCTTCCCCTTCCTCCTCTCTTCTCTTTCGCCGCAAGTATCGTCTCAATTGCCGCCGCAGACCTTCTAGAAGCTTCACCGTCCGTGCCTCCTCCGCTGCTAGTGATGATTCAGTGGTGACTCTGCTTGACTACGGTGCCGGCAATGTTCGGAGTGTCAGGAACGCCATTCGCCGCCTCGGCTTCCAAGTCAAAGATGTCCAAACTCCCAAGGATATTTTGAATGCCAACCGCCTAATCTTTCCTGGTGTGGGGGCTTTCGCTGCGGCAATGGATGTGCTGAGCAAGAATGGGATGGCTGAAGCGCTCTGTGAATATATTGAGAAAGATCGGCCGTTTCTAGGCATTTGTCTTGGACTTCAGCTCCTTTTTGAATCCAGTGAGGAAAAGGGACAAGTGAGTGGTCTTGGCTTGATACCTGGAGTGGTTGGGCGTTTTGACTCATCAAATGGTGTTCGAGTGCCACATATCGGGTGGAATGCTTTGCAGATTAGGGAAGACTCTTTAATTTTGGATGATGTTGGAACCCATCACGTCTATTTCGTCCACTCTTACAGAGCAATGCCATCGGATGATAACAAAGAATGGGTTTCATCTACCTGCAACTATGGTGACAATTTCATTGCATCTGTTAGAAGGGGAAATGTGCATGCAGTTCAATTCCACCCTGAGAAAAGTGGAGGCAAGATATCATTTGCTCTCTCTTGTTTGTTAATTATTGTTCAAGATGTACAGGAATTACAACAGAAGCCCACTGAAGGGAAGGGTTCCAAACTTGCAAAGAGGGTTATTGCTTGTCTGGATGTGAGGACGAATGACAAAGGAGATCTTGTTGTAACCAAAGGAGACCAATATGACGTAAGAGAGCATACAAAAGAGAATGAGGTGAGAGAACTTGGAAAGCCTGTGGAGCTTGCTGGACAGTATTACACTGATGGAGCTGATGAGGTCAGCTTTCTGAATATTACCGGCTTCCGGGACTTCCCTTTGGGTGACTTGCCTATGCTACAGGTATTGAGATATGCATCAGAAAATGTTTTCGTGCCATTAACAGTTGGAGGTGGCATTAGAGATTTTACCGATGCAACTGGCAGGCACTATTCTAGTTTGGAAGTTGCTTCAGAATATTTTAGATGTGGGGCTGATAAGATCTCCATTGGAAGTGATGCAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGTGAAAAGTGGAAAAAGCAGCTTAGAGCAGATATCCAGAGTTTACGGAAATCAGGCTGTGGTTGTAAGCATTGATCCTCGCAGAGTGTATATTAAAAATCTAAATGATGTAAAGTACAAGACTATAAGAGTAACAAATCCAGGTCCAAATGGCGAAGAATATGCTTGGTATCAGTGTACAGTTAGCGGTGGGCGGGAAGGCAGACCAATTGGAGCGTATGAGCTTGCAAAAGCAGTTGAGGAGCTTGGAGCTGGAGAAATTCTGCTAAACTGCATTGATTGCGATGGTCAAGGGAAAGGATTTGATATAGATCTAGTAAAGCTCATCTCTGATGCTGTGACCATTCCCGTGATTGCAAGTAGTGGTGCTGGTGGTGTTGAACACTTCTCAGAGGTTTTCATGAAAACAAATGCATCTGCTGCCCTCGCTGCTGGCATATTCCATCGCAAGGAGGTGCCAATTCATTCTGTAAAGGAGCACTTGCTAAAGGAAGGCATAGAAGTCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

587

Amino Acids

64.25

Weight (kDa)

6.66

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 58 - 244 3.6e-23 Glutamine amidotransferase class-I
SNO PF01174 64 - 243 1.1e-07 SNO glutamine amidotransferase family
GATase_3 PF07685 79 - 157 2.9e-08 CobB/CobQ-like glutamine amidotransferase domain
His_biosynth PF00977 278 - 569 1.7e-41 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 656
AccB1I GGYRCC 3 cut(s) 7, 185, 1705
AclWI GGATC 1 cut(s) 1319
AcoI YGGCCR 1 cut(s) 380
AcsI RAATTY 1 cut(s) 1509
AcuI CTGAAG 4 cut(s) 369, 392, 825, 1152
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 3 cut(s) 780, 1367, 1429
AfeI AGCGCT 1 cut(s) 354
AfiI CCNNNNNNNGG 7 cut(s) 299, 389, 518, 806, 807, 1023, 1699
AgsI TTSAA 5 cut(s) 271, 422, 707, 773, 1631
AjiI CACGTC 1 cut(s) 583
AjnI CCWGG 3 cut(s) 292, 466, 1390
Alw21I GWGCWC 1 cut(s) 1731
Alw26I GTCTC 2 cut(s) 98, 888
AlwI GGATC 1 cut(s) 1319
AlwNI CAGNNNCTG 1 cut(s) 1761
Aor51HI AGCGCT 1 cut(s) 354
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 7 cut(s) 37, 146, 311, 1226, 1269, 1670, 1679
ApoI RAATTY 1 cut(s) 1509
AspLEI GCGC 2 cut(s) 10, 355
AspS9I GGNCC 1 cut(s) 1394
AsuC2I CCSGG 1 cut(s) 1025
AsuHPI GGTGA 5 cut(s) 118, 178, 671, 946, 1052
AvaII GGWCC 1 cut(s) 1394
BanI GGYRCC 3 cut(s) 7, 185, 1705
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 1731
BbvI GCAGC 7 cut(s) 24, 133, 298, 1213, 1281, 1657, 1666
BccI CCATC 6 cut(s) 337, 585, 622, 977, 1529, 1701
BceAI ACGGC 2 cut(s) 25, 367
BcgI CGANNNNNNTGC 4 cut(s) 597, 631, 1394, 1428
BciT130I CCWGG 3 cut(s) 294, 468, 1392
BcnI CCSGG 1 cut(s) 1025
BcoDI GTCTC 2 cut(s) 98, 888
BfaI CTAG 5 cut(s) 117, 150, 389, 1152, 1565
BfmI CTRYAG 2 cut(s) 1055, 1227
BfoI RGCGCY 2 cut(s) 11, 356
BfuAI ACCTGC 1 cut(s) 656
BglI GCCNNNNNGGC 1 cut(s) 228
BglII AGATCT 3 cut(s) 874, 1197, 1561
BlpI GCTNAGC 1 cut(s) 329
Bme1390I CCNGG 4 cut(s) 294, 468, 1025, 1392
Bme18I GGWCC 1 cut(s) 1394
BmgBI CACGTC 1 cut(s) 583
BmgT120I GGNCC 1 cut(s) 1394
BmiI GGNNCC 5 cut(s) 9, 187, 574, 817, 1707
BmrFI CCNGG 4 cut(s) 294, 468, 1025, 1392
BmsI GCATC 6 cut(s) 683, 1082, 1120, 1204, 1573, 1673
BpiI GAAGAC 1 cut(s) 552
BpmI CTGGAG 3 cut(s) 489, 1269, 1524
Bpu1102I GCTNAGC 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 1025
BsaBI GATNNNNATC 2 cut(s) 1072, 1560
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 888
BsaJI CCNNGG 2 cut(s) 226, 258
BsaXI ACNNNNNCTCC 4 cut(s) 396, 426, 864, 894
Bsc4I CCNNNNNNNGG 7 cut(s) 299, 389, 518, 806, 807, 1023, 1699
Bse118I RCCGGY 2 cut(s) 188, 1016
Bse1I ACTGG 3 cut(s) 426, 1141, 1252
Bse3DI GCAATG 4 cut(s) 199, 324, 615, 669
Bse8I GATNNNNATC 2 cut(s) 1072, 1560
BseBI CCWGG 3 cut(s) 294, 468, 1392
BseDI CCNNGG 2 cut(s) 226, 258
BseGI GGATG 5 cut(s) 328, 348, 568, 625, 856
BseJI GATNNNNATC 2 cut(s) 1072, 1560
BseLI CCNNNNNNNGG 7 cut(s) 299, 389, 518, 806, 807, 1023, 1699
BseMI GCAATG 4 cut(s) 199, 324, 615, 669
BseMII CTCAG 3 cut(s) 320, 708, 1653
BseNI ACTGG 3 cut(s) 426, 1141, 1252
BseRI GAGGAG 3 cut(s) 55, 130, 1505
BseX3I CGGCCG 1 cut(s) 380
BseXI GCAGC 7 cut(s) 24, 133, 298, 1213, 1281, 1657, 1666
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 1 cut(s) 382
BshNI GGYRCC 3 cut(s) 7, 185, 1705
BsiEI CGRYCG 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 1731
BsiSI CCGG 3 cut(s) 189, 1017, 1024
BslFI GGGAC 2 cut(s) 453, 1040
BslI CCNNNNNNNGG 7 cut(s) 299, 389, 518, 806, 807, 1023, 1699
BsmAI GTCTC 2 cut(s) 98, 888
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 2 cut(s) 453, 1040
BsmI GAATGC 2 cut(s) 277, 532
BsnI GGCC 1 cut(s) 382
Bso31I GGTCTC 1 cut(s) 888
Bsp1286I GDGCHC 1 cut(s) 1731
Bsp1407I TGTACA 2 cut(s) 778, 1427
Bsp143I GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
Bsp1720I GCTNAGC 1 cut(s) 329
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 3 cut(s) 321, 709, 1652
BspHI TCATGA 1 cut(s) 1650
BspLI GGNNCC 5 cut(s) 9, 187, 574, 817, 1707
BspMAI CTGCAG 1 cut(s) 1231
BspMI ACCTGC 1 cut(s) 656
BspPI GGATC 1 cut(s) 1319
BspT107I GGYRCC 3 cut(s) 7, 185, 1705
BspTNI GGTCTC 1 cut(s) 888
BsrDI GCAATG 4 cut(s) 199, 324, 615, 669
BsrFI RCCGGY 2 cut(s) 188, 1016
BsrGI TGTACA 2 cut(s) 778, 1427
BsrI ACTGG 3 cut(s) 426, 1141, 1252
BssAI RCCGGY 2 cut(s) 188, 1016
BssECI CCNNGG 2 cut(s) 226, 258
BssMI GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 3 cut(s) 294, 468, 1392
Bst4CI ACNGT 5 cut(s) 130, 185, 972, 1102, 1432
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 3 cut(s) 1223, 1530, 1670
BstAUI TGTACA 2 cut(s) 778, 1427
BstC8I GCNNGC 6 cut(s) 190, 699, 963, 1142, 1476, 1684
BstDEI CTNAG 4 cut(s) 329, 717, 1273, 1639
BstF5I GGATG 5 cut(s) 328, 348, 568, 625, 856
BstH2I RGCGCY 2 cut(s) 11, 356
BstHHI GCGC 2 cut(s) 10, 355
BstKTI GATC 6 cut(s) 50, 379, 877, 1200, 1327, 1564
BstMAI GTCTC 2 cut(s) 98, 888
BstMBI GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 9 cut(s) 143, 228, 311, 958, 1223, 1530, 1670, 1676, 1679
BstNI CCWGG 3 cut(s) 294, 468, 1392
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 4 cut(s) 292, 466, 1023, 1390
BstSFI CTRYAG 2 cut(s) 1055, 1227
BstV1I GCAGC 7 cut(s) 24, 133, 298, 1213, 1281, 1657, 1666
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 3 cut(s) 874, 1197, 1561
BstYI RGATCY 3 cut(s) 874, 1197, 1561
BstZI CGGCCG 1 cut(s) 380
BsuRI GGCC 1 cut(s) 382
BtgZI GCGATG 2 cut(s) 1548, 1679
BtrI CACGTC 1 cut(s) 583
BtsCI GGATG 5 cut(s) 328, 348, 568, 625, 856
BtsIMutI CAGTG 5 cut(s) 168, 433, 801, 978, 1430
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 6 cut(s) 190, 699, 963, 1142, 1476, 1684
CaiI CAGNNNCTG 1 cut(s) 1761
CciI TCATGA 1 cut(s) 1650
CfoI GCGC 2 cut(s) 10, 355
Cfr10I RCCGGY 2 cut(s) 188, 1016
Cfr13I GGNCC 1 cut(s) 1394
Csp6I GTAC 3 cut(s) 779, 1366, 1428
CviAII CATG 2 cut(s) 698, 1651
CviQI GTAC 3 cut(s) 779, 1366, 1428
DdeI CTNAG 4 cut(s) 329, 717, 1273, 1639
DinI GGCGCC 1 cut(s) 9
DpnI GATC 6 cut(s) 49, 378, 876, 1199, 1326, 1563
DpnII GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
EaeI YGGCCR 1 cut(s) 380
EagI CGGCCG 1 cut(s) 380
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
EclXI CGGCCG 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 888
Eco32I GATATC 2 cut(s) 738, 1284
Eco47I GGWCC 1 cut(s) 1394
Eco47III AGCGCT 1 cut(s) 354
Eco52I CGGCCG 1 cut(s) 380
Eco57I CTGAAG 4 cut(s) 369, 392, 825, 1152
EcoRII CCWGG 3 cut(s) 292, 466, 1390
EcoRV GATATC 2 cut(s) 738, 1284
EcoT14I CCWWGG 1 cut(s) 258
EcoT22I ATGCAT 2 cut(s) 1075, 1666
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 2 cut(s) 701, 1654
FalI AAGNNNNNCTT 4 cut(s) 861, 893, 1715, 1747
FaqI GGGAC 2 cut(s) 453, 1040
FatI CATG 2 cut(s) 697, 1650
FauI CCCGC 1 cut(s) 1437
FokI GGATG 5 cut(s) 335, 355, 575, 632, 863
FspBI CTAG 5 cut(s) 117, 150, 389, 1152, 1565
GlaI GCGC 2 cut(s) 9, 354
GsuI CTGGAG 3 cut(s) 489, 1269, 1524
HaeII RGCGCY 2 cut(s) 11, 356
HaeIII GGCC 1 cut(s) 382
HapII CCGG 3 cut(s) 189, 1017, 1024
HhaI GCGC 2 cut(s) 10, 355
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 2 cut(s) 701, 1654
Hin6I GCGC 2 cut(s) 8, 353
HinP1I GCGC 2 cut(s) 8, 353
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 6 cut(s) 158, 169, 422, 488, 548, 1758
HpaII CCGG 3 cut(s) 189, 1017, 1024
HphI GGTGA 5 cut(s) 118, 178, 671, 946, 1052
Hpy166II GTNNAC 3 cut(s) 595, 1294, 1428
Hpy188III TCNNGA 6 cut(s) 117, 208, 773, 848, 1287, 1651
Hpy8I GTNNAC 3 cut(s) 595, 1294, 1428
HpyAV CCTTC 7 cut(s) 69, 123, 678, 800, 805, 1442, 1736
HpyCH4III ACNGT 5 cut(s) 130, 185, 972, 1102, 1432
HpyCH4IV ACGT 2 cut(s) 582, 906
HpyF10VI GCNNNNNNNGC 9 cut(s) 143, 228, 311, 958, 1223, 1530, 1670, 1676, 1679
HpyF3I CTNAG 4 cut(s) 329, 717, 1273, 1639
HpySE526I ACGT 2 cut(s) 582, 906
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 2 cut(s) 701, 1654
HspAI GCGC 2 cut(s) 8, 353
KasI GGCGCC 1 cut(s) 7
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Kzo9I GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
LmnI GCTCC 7 cut(s) 417, 958, 986, 1463, 1492, 1499, 1726
Lsp1109I GCAGC 7 cut(s) 24, 133, 298, 1213, 1281, 1657, 1666
LweI GCATC 6 cut(s) 683, 1082, 1120, 1204, 1573, 1673
MaeI CTAG 5 cut(s) 117, 150, 389, 1152, 1565
MaeII ACGT 2 cut(s) 582, 906
MaeIII GTNAC 6 cut(s) 166, 659, 883, 1040, 1381, 1588
MalI GATC 6 cut(s) 49, 378, 876, 1199, 1326, 1563
MboI GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
MboII GAAGA 4 cut(s) 62, 557, 1244, 1418
MfeI CAATTG 3 cut(s) 15, 97, 1458
MflI RGATCY 3 cut(s) 874, 1197, 1561
MhlI GDGCHC 1 cut(s) 1731
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 3 cut(s) 163, 482, 542
MmeI TCCRAC 2 cut(s) 550, 1084
Mph1103I ATGCAT 2 cut(s) 1075, 1666
MroNI GCCGGC 1 cut(s) 188
MseI TTAA 5 cut(s) 554, 761, 1097, 1241, 1344
MslI CAYNNNNRTG 1 cut(s) 1598
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 3 cut(s) 189, 1017, 1024
MspR9I CCNGG 4 cut(s) 294, 468, 1025, 1392
MunI CAATTG 3 cut(s) 15, 97, 1458
Mva1269I GAATGC 2 cut(s) 277, 532
MvaI CCWGG 3 cut(s) 294, 468, 1392
MwoI GCNNNNNNNGC 9 cut(s) 143, 228, 311, 958, 1223, 1530, 1670, 1676, 1679
NaeI GCCGGC 1 cut(s) 190
NarI GGCGCC 1 cut(s) 8
NciI CCSGG 1 cut(s) 1025
NdeII GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 2 cut(s) 701, 1654
NlaIV GGNNCC 5 cut(s) 9, 187, 574, 817, 1707
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 4 cut(s) 166, 659, 1040, 1588
NsiI ATGCAT 2 cut(s) 1075, 1666
NspI RCATGY 1 cut(s) 701
PaeI GCATGC 1 cut(s) 701
PagI TCATGA 1 cut(s) 1650
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 2 cut(s) 277, 532
PdiI GCCGGC 1 cut(s) 190
PfeI GAWTC 3 cut(s) 158, 422, 1758
PfoI TCCNGGA 1 cut(s) 1023
PleI GAGTC 3 cut(s) 163, 482, 542
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 3 cut(s) 163, 482, 542
Psp6I CCWGG 3 cut(s) 292, 466, 1390
PspGI CCWGG 3 cut(s) 292, 466, 1390
PspN4I GGNNCC 5 cut(s) 9, 187, 574, 817, 1707
PspPI GGNCC 1 cut(s) 1394
PstI CTGCAG 1 cut(s) 1231
PstNI CAGNNNCTG 1 cut(s) 1761
PsuI RGATCY 3 cut(s) 874, 1197, 1561
RsaI GTAC 3 cut(s) 780, 1367, 1429
RsaNI GTAC 3 cut(s) 779, 1366, 1428
RseI CAYNNNNRTG 1 cut(s) 1598
SaqAI TTAA 5 cut(s) 554, 761, 1097, 1241, 1344
Sau3AI GATC 6 cut(s) 47, 376, 874, 1197, 1324, 1561
Sau96I GGNCC 1 cut(s) 1394
SchI GAGTC 3 cut(s) 163, 482, 542
ScrFI CCNGG 4 cut(s) 294, 468, 1025, 1392
SduI GDGCHC 1 cut(s) 1731
SfaNI GCATC 6 cut(s) 683, 1082, 1120, 1204, 1573, 1673
SfcI CTRYAG 2 cut(s) 1055, 1227
SfoI GGCGCC 1 cut(s) 9
SinI GGWCC 1 cut(s) 1394
SmiMI CAYNNNNRTG 1 cut(s) 1598
SphI GCATGC 1 cut(s) 701
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 3 cut(s) 1012, 1175, 1238
SspMI CTAG 5 cut(s) 117, 150, 389, 1152, 1565
StyD4I CCNGG 4 cut(s) 292, 466, 1023, 1390
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 5 cut(s) 130, 185, 972, 1102, 1432
TaiI ACGT 2 cut(s) 585, 909
TaqI TCGA 1 cut(s) 505
TatI WGTACW 3 cut(s) 778, 1365, 1427
TauI GCSGC 6 cut(s) 13, 84, 105, 108, 225, 317
TfiI GAWTC 3 cut(s) 158, 422, 1758
Tru1I TTAA 5 cut(s) 554, 761, 1097, 1241, 1344
Tru9I TTAA 5 cut(s) 554, 761, 1097, 1241, 1344
TscAI CASTG 5 cut(s) 168, 433, 808, 985, 1430
TseFI GTSAC 4 cut(s) 166, 659, 1040, 1588
TseI GCWGC 7 cut(s) 37, 146, 311, 1226, 1269, 1670, 1679
Tsp45I GTSAC 4 cut(s) 166, 659, 1040, 1588
TspDTI ATGAA 5 cut(s) 630, 658, 1639, 1667, 1703
TspGWI ACGGA 2 cut(s) 122, 1311
TspRI CASTG 5 cut(s) 168, 433, 808, 985, 1430
VpaK11BI GGWCC 1 cut(s) 1394
XapI RAATTY 1 cut(s) 1509
XbaI TCTAGA 1 cut(s) 116
XceI RCATGY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 519
XspI CTAG 5 cut(s) 117, 150, 389, 1152, 1565
Zsp2I ATGCAT 2 cut(s) 1075, 1666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.