RLG00000008296

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003239
Physical Location & Seq
Reverse (-)
28486260 .. 28489344
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008296

Sequence Viewer

Length: 843 bp
ATGTATAGTGCAAAGCATCCTCTAGTCCTCTGCACTGATGATTCTGGGGTCTTTTCTACCAGCCCTTCCAACGAGTACAACCTTGCTGCTTCTGCATTTGGTCTTGGAAGGAGGGAAATATTTCAGCTAACGAGAAATTCGTGCAAGTTTATATTTGCAGATGATGGAGTCAGCTTTCTGCATATTAATGGTTTCTGGGACTCCCCTTTGGGTGCCTTGCCTACGCTACAGGTATTAAGATATGCATCAGAAAATGTTTTTGTGCCATTAACAGTTGCAGGTAGCATTAGAGATTTTACCGATGCAACTAGCAGATGTGGGGCTGATAAGATCTCCATCGGAAGTGACGCAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGCTGTGGTTGTAAGCATTGATCCTTGCAAAGTGTACGTTAAAAATCAAAATGATGTTGAGTTCGCGACTGTAAGGGTAACAAACCCAGTCCTGATTTTTGTCCTTAAGGTTAGCGGTGGGTGGGAAAGCCAACGAATCGGAGCTTATGAGCTTACAAAAGCAGTTGAGGAGCTTGGTGCTGGAGAAATTCTGCTAAACTGCATTGATTATGAAGGTAAACTCAGGGAAAGGGGATTTGATATAGATTTAGTAAAGCTGATCTCTAATGTGGTGACCATTCCCATGCTTGCAAGTAGTGGTGCTGGTGTTGTTGAAAACTTCTCAGAGGTTCTCATGAAAGCAAATGCGTCTGCTGCCTTTGCTGCTGGCATTTTTCATCCCAAGGAGATTATGATTGTTCCAGGGATTCATTCTGTAAAGGATCACTTGCTAAAGGAAGGCATAGAAGTCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

281

Amino Acids

30.36

Weight (kDa)

5.2

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 53 - 258 4.8e-22 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 212
AccII CGCG 1 cut(s) 449
AciI CCGC 1 cut(s) 498
AclWI GGATC 2 cut(s) 398, 813
AcsI RAATTY 2 cut(s) 136, 570
AfaI GTAC 2 cut(s) 77, 419
AflII CTTAAG 1 cut(s) 488
AgsI TTSAA 1 cut(s) 698
AjnI CCWGG 1 cut(s) 784
AluBI AGCT 7 cut(s) 127, 174, 386, 527, 535, 556, 640
AluI AGCT 7 cut(s) 127, 174, 386, 527, 535, 556, 640
AlwI GGATC 2 cut(s) 398, 813
AlwNI CAGNNNCTG 1 cut(s) 840
ApeKI GCWGC 4 cut(s) 86, 359, 737, 746
ApoI RAATTY 2 cut(s) 136, 570
AseI ATTAAT 1 cut(s) 186
Asp700I GAANNNNTTC 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 667
BanI GGYRCC 1 cut(s) 212
BbvI GCAGC 4 cut(s) 73, 346, 724, 733
BccI CCATC 2 cut(s) 158, 344
BciT130I CCWGG 1 cut(s) 786
BfaI CTAG 2 cut(s) 23, 309
BfmI CTRYAG 2 cut(s) 227, 360
BfrI CTTAAG 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 269
BglII AGATCT 1 cut(s) 330
BisI GCNGC 4 cut(s) 87, 360, 738, 747
BlsI GCNGC 4 cut(s) 88, 361, 739, 748
Bme1390I CCNGG 1 cut(s) 786
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 1 cut(s) 786
BmrI ACTGGG 1 cut(s) 464
BmsI GCATC 3 cut(s) 25, 254, 292
BmuI ACTGGG 1 cut(s) 464
BpmI CTGGAG 2 cut(s) 402, 585
BsaBI GATNNNNATC 2 cut(s) 244, 335
BsaJI CCNNGG 2 cut(s) 765, 785
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bse1I ACTGG 2 cut(s) 385, 470
Bse8I GATNNNNATC 2 cut(s) 244, 335
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 765, 785
BseGI GGATG 2 cut(s) 16, 760
BseJI GATNNNNATC 2 cut(s) 244, 335
BseMII CTCAG 2 cut(s) 619, 720
BseNI ACTGG 2 cut(s) 385, 470
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 4 cut(s) 73, 346, 724, 733
BsgI GTGCAG 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 449
BshNI GGYRCC 1 cut(s) 212
BslFI GGGAC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 212
Bsp143I GATC 4 cut(s) 330, 403, 642, 805
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 1 cut(s) 498
BspCNI CTCAG 2 cut(s) 618, 719
BspFNI CGCG 1 cut(s) 449
BspHI TCATGA 1 cut(s) 717
BspLI GGNNCC 1 cut(s) 214
BspMAI CTGCAG 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 2 cut(s) 398, 813
BspT107I GGYRCC 1 cut(s) 212
BspTI CTTAAG 1 cut(s) 488
BsrI ACTGG 2 cut(s) 385, 470
BssECI CCNNGG 2 cut(s) 765, 785
BssMI GATC 4 cut(s) 330, 403, 642, 805
BssT1I CCWWGG 1 cut(s) 765
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 2 cut(s) 274, 454
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 672, 751
BstDEI CTNAG 2 cut(s) 605, 706
BstEII GGTNACC 1 cut(s) 655
BstF5I GGATG 2 cut(s) 16, 760
BstFNI CGCG 1 cut(s) 449
BstKTI GATC 4 cut(s) 333, 406, 645, 808
BstMBI GATC 4 cut(s) 330, 403, 642, 805
BstMWI GCNNNNNNNGC 5 cut(s) 92, 356, 737, 743, 746
BstNI CCWGG 1 cut(s) 786
BstPI GGTNACC 1 cut(s) 655
BstSCI CCNGG 1 cut(s) 784
BstSFI CTRYAG 2 cut(s) 227, 360
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 4 cut(s) 73, 346, 724, 733
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BtsCI GGATG 2 cut(s) 16, 760
BtsIMutI CAGTG 1 cut(s) 33
BtuMI TCGCGA 1 cut(s) 449
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 672, 751
CaiI CAGNNNCTG 1 cut(s) 840
CciI TCATGA 1 cut(s) 717
CseI GACGC 2 cut(s) 356, 720
Csp6I GTAC 2 cut(s) 76, 418
CviAII CATG 2 cut(s) 667, 718
CviQI GTAC 2 cut(s) 76, 418
DdeI CTNAG 2 cut(s) 605, 706
DpnI GATC 4 cut(s) 332, 405, 644, 807
DpnII GATC 4 cut(s) 330, 403, 642, 805
Eco130I CCWWGG 1 cut(s) 765
Eco91I GGTNACC 1 cut(s) 655
EcoO65I GGTNACC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 784
EcoT14I CCWWGG 1 cut(s) 765
EcoT22I ATGCAT 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 765
FaeI CATG 2 cut(s) 670, 721
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 1 cut(s) 212
FatI CATG 2 cut(s) 666, 717
Fnu4HI GCNGC 4 cut(s) 87, 360, 738, 747
FokI GGATG 2 cut(s) 3, 747
Fsp4HI GCNGC 4 cut(s) 87, 360, 738, 747
FspBI CTAG 2 cut(s) 23, 309
GluI GCNGC 4 cut(s) 87, 360, 738, 747
GsuI CTGGAG 2 cut(s) 402, 585
HgaI GACGC 2 cut(s) 356, 720
Hin1II CATG 2 cut(s) 670, 721
HinfI GANTC 6 cut(s) 41, 168, 200, 519, 790, 837
HphI GGTGA 1 cut(s) 667
Hpy166II GTNNAC 2 cut(s) 418, 602
Hpy188I TCNGA 6 cut(s) 250, 341, 524, 709, 836, 842
Hpy188III TCNNGA 3 cut(s) 448, 475, 718
Hpy8I GTNNAC 2 cut(s) 418, 602
HpyAV CCTTC 4 cut(s) 75, 102, 590, 815
HpyCH4III ACNGT 2 cut(s) 274, 454
HpyCH4IV ACGT 1 cut(s) 420
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 356, 737, 743, 746
HpyF3I CTNAG 2 cut(s) 605, 706
HpySE526I ACGT 1 cut(s) 420
Hsp92II CATG 2 cut(s) 670, 721
Kzo9I GATC 4 cut(s) 330, 403, 642, 805
LmnI GCTCC 3 cut(s) 383, 524, 553
Lsp1109I GCAGC 4 cut(s) 73, 346, 724, 733
LweI GCATC 3 cut(s) 25, 254, 292
MaeI CTAG 2 cut(s) 23, 309
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 3 cut(s) 344, 460, 655
MalI GATC 4 cut(s) 332, 405, 644, 807
MboI GATC 4 cut(s) 330, 403, 642, 805
MboII GAAGA 1 cut(s) 377
MflI RGATCY 1 cut(s) 330
MluCI AATT 2 cut(s) 136, 570
MlyI GAGTC 2 cut(s) 177, 194
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 5 cut(s) 30, 38, 105, 544, 703
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 6 cut(s) 186, 236, 269, 374, 423, 489
MslI CAYNNNNRTG 2 cut(s) 186, 665
MspCI CTTAAG 1 cut(s) 488
MspR9I CCNGG 1 cut(s) 786
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 5 cut(s) 92, 356, 737, 743, 746
NdeII GATC 4 cut(s) 330, 403, 642, 805
NlaIII CATG 2 cut(s) 670, 721
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 2 cut(s) 344, 655
NruI TCGCGA 1 cut(s) 449
NsiI ATGCAT 1 cut(s) 247
PagI TCATGA 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 4 cut(s) 41, 519, 790, 837
PkrI GCNGC 4 cut(s) 88, 361, 739, 748
PleI GAGTC 2 cut(s) 176, 194
PpsI GAGTC 2 cut(s) 176, 194
PshBI ATTAAT 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 655
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 1 cut(s) 214
PstI CTGCAG 1 cut(s) 364
PstNI CAGNNNCTG 1 cut(s) 840
PsuI RGATCY 1 cut(s) 330
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 2 cut(s) 77, 419
RsaNI GTAC 2 cut(s) 76, 418
RseI CAYNNNNRTG 2 cut(s) 186, 665
SaqAI TTAA 6 cut(s) 186, 236, 269, 374, 423, 489
SatI GCNGC 4 cut(s) 87, 360, 738, 747
Sau3AI GATC 4 cut(s) 330, 403, 642, 805
SchI GAGTC 2 cut(s) 177, 194
ScrFI CCNGG 1 cut(s) 786
SfaNI GCATC 3 cut(s) 25, 254, 292
SfcI CTRYAG 2 cut(s) 227, 360
SmiMI CAYNNNNRTG 2 cut(s) 186, 665
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 2 cut(s) 136, 570
SsiI CCGC 1 cut(s) 498
SspI AATATT 2 cut(s) 120, 371
SspMI CTAG 2 cut(s) 23, 309
StyD4I CCNGG 1 cut(s) 784
StyI CCWWGG 1 cut(s) 765
TaaI ACNGT 2 cut(s) 274, 454
TaiI ACGT 1 cut(s) 423
TasI AATT 2 cut(s) 136, 570
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 4 cut(s) 41, 519, 790, 837
Tru1I TTAA 6 cut(s) 186, 236, 269, 374, 423, 489
Tru9I TTAA 6 cut(s) 186, 236, 269, 374, 423, 489
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 2 cut(s) 344, 655
TseI GCWGC 4 cut(s) 86, 359, 737, 746
Tsp45I GTSAC 2 cut(s) 344, 655
TspDTI ATGAA 4 cut(s) 609, 734, 749, 782
TspRI CASTG 1 cut(s) 40
Vha464I CTTAAG 1 cut(s) 488
VspI ATTAAT 1 cut(s) 186
XapI RAATTY 2 cut(s) 136, 570
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 2 cut(s) 23, 309
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.