Rh4BG172600

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003239
Physical Location & Seq
Forward (+)
30845360 .. 30849054
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG172600.1

Sequence Viewer

Length: 744 bp
ATGAATCAAAAGAGAGATATGAATTTATCAGGTCGTTGGACGATCTCGTTGAAACAGGTCAGCTTTCTGCATATTAATGGTTTCTGGGACTCCCCTTTGGGTGCCTTGCCTATGCTACAGGTATTAAGATATGCATCAGAAAATGTTTTTGTGCCATTAACAGTTGCAGGTAGCATTAGAGATTTTACCGATGCAACTAGCAGATGTGGGGCTGATAAGATCTCCATCGGAAGTGACGCAGTTTATGCTGCAGAAGAATATTTAAGAACTGGATTGAAAAGTGGAAAAAGTAGCTTCGAGCAGATATCTAGAGTTTATGGAAATCAGATTGAATTATTATTTTTTTTTTTTTACTTTATTGGACTATATGTTGCAGCTGTGGTTGTAAGCATTGATCCTTGCAAAGTGTACGTTAAAAATCAAAATGATGTTGAGTTCGAGACTATAAGGGTAACAAACCCAGTCTTGAATTTTGTCCTTAAGGTTAGCGGCGGGCGGGAAAGCCAACAAATCGGAGCTTATGAGCTTACAAAAGCAGTTGAGGAGCTTGGTGCTGGAGAAATTCTGCTAAACTGCATTGATTATGAAGGTAAAGTTCAGTTTTTGCGCTCCTTATTCTTGCTTATGAAAACCACTTATATGAGAATTGCAAAGAGAAATTTGTCTATACATTTAATCAATGGCATGTTAGTTTATCACTCATCACGGTCTGAGATAATGCCAAAATGTTGGAAGGTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

247

Amino Acids

27.91

Weight (kDa)

8.8

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 101
AciI CCGC 3 cut(s) 489, 492, 496
AclWI GGATC 1 cut(s) 389
AcsI RAATTY 4 cut(s) 22, 469, 561, 658
AfaI GTAC 1 cut(s) 410
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 4 cut(s) 52, 277, 332, 469
AluBI AGCT 6 cut(s) 63, 294, 377, 518, 526, 547
AluI AGCT 6 cut(s) 63, 294, 377, 518, 526, 547
Alw26I GTCTC 1 cut(s) 434
AlwI GGATC 1 cut(s) 389
ApeKI GCWGC 2 cut(s) 248, 374
ApoI RAATTY 4 cut(s) 22, 469, 561, 658
AseI ATTAAT 1 cut(s) 75
AspLEI GCGC 1 cut(s) 609
BanI GGYRCC 1 cut(s) 101
BbvI GCAGC 2 cut(s) 235, 386
BccI CCATC 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 3 cut(s) 198, 309, 742
BfmI CTRYAG 2 cut(s) 116, 249
BfrI CTTAAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 158
BglII AGATCT 1 cut(s) 219
BisI GCNGC 3 cut(s) 249, 375, 490
BlsI GCNGC 3 cut(s) 250, 376, 491
BmiI GGNNCC 1 cut(s) 103
BmrI ACTGGG 1 cut(s) 455
BmsI GCATC 2 cut(s) 143, 181
BmuI ACTGGG 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 576
BsaBI GATNNNNATC 2 cut(s) 133, 224
Bse1I ACTGG 2 cut(s) 274, 461
Bse8I GATNNNNATC 2 cut(s) 133, 224
BseJI GATNNNNATC 2 cut(s) 133, 224
BseMII CTCAG 1 cut(s) 702
BseNI ACTGG 2 cut(s) 274, 461
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 2 cut(s) 235, 386
BshNI GGYRCC 1 cut(s) 101
BslFI GGGAC 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 101
Bsp143I GATC 3 cut(s) 42, 219, 394
BspACI CCGC 3 cut(s) 489, 492, 496
BspCNI CTCAG 1 cut(s) 703
BspLI GGNNCC 1 cut(s) 103
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 158
BspPI GGATC 1 cut(s) 389
BspT107I GGYRCC 1 cut(s) 101
BspTI CTTAAG 1 cut(s) 479
BsrI ACTGG 2 cut(s) 274, 461
BssMI GATC 3 cut(s) 42, 219, 394
Bst4CI ACNGT 2 cut(s) 163, 708
BstAFI CTTAAG 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 494
BstDEI CTNAG 1 cut(s) 711
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 3 cut(s) 45, 222, 397
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 3 cut(s) 42, 219, 394
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 688
BstSFI CTRYAG 2 cut(s) 116, 249
BstV1I GCAGC 2 cut(s) 235, 386
BstX2I RGATCY 1 cut(s) 219
BstXI CCANNNNNNTGG 1 cut(s) 729
BstYI RGATCY 1 cut(s) 219
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 494
CfoI GCGC 1 cut(s) 609
CseI GACGC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 409
CviAII CATG 1 cut(s) 685
CviJI RGCY 8 cut(s) 63, 212, 294, 377, 504, 518, 526, 547
CviKI_1 RGCY 8 cut(s) 63, 212, 294, 377, 504, 518, 526, 547
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 1 cut(s) 711
DpnI GATC 3 cut(s) 44, 221, 396
DpnII GATC 3 cut(s) 42, 219, 394
Eco32I GATATC 1 cut(s) 306
EcoRV GATATC 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 136
FaeI CATG 1 cut(s) 688
FaqI GGGAC 1 cut(s) 101
FatI CATG 1 cut(s) 684
FauI CCCGC 2 cut(s) 485, 489
Fnu4HI GCNGC 3 cut(s) 249, 375, 490
Fsp4HI GCNGC 3 cut(s) 249, 375, 490
FspBI CTAG 3 cut(s) 198, 309, 742
GlaI GCGC 1 cut(s) 608
GluI GCNGC 3 cut(s) 249, 375, 490
GsuI CTGGAG 1 cut(s) 576
HgaI GACGC 1 cut(s) 245
HhaI GCGC 1 cut(s) 609
Hin1II CATG 1 cut(s) 688
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HinfI GANTC 2 cut(s) 4, 89
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 5 cut(s) 139, 230, 327, 515, 712
Hpy188III TCNNGA 3 cut(s) 309, 439, 466
Hpy8I GTNNAC 1 cut(s) 409
HpyAV CCTTC 2 cut(s) 581, 727
HpyCH4III ACNGT 2 cut(s) 163, 708
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 9 cut(s) 70, 134, 167, 194, 251, 374, 402, 576, 650
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 711
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 1 cut(s) 688
HspAI GCGC 1 cut(s) 607
Kzo9I GATC 3 cut(s) 42, 219, 394
LmnI GCTCC 3 cut(s) 515, 544, 614
LpnPI CCDG 8 cut(s) 15, 41, 70, 104, 153, 255, 474, 540
Lsp1109I GCAGC 2 cut(s) 235, 386
LweI GCATC 2 cut(s) 143, 181
MaeI CTAG 3 cut(s) 198, 309, 742
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 233, 451
MalI GATC 3 cut(s) 44, 221, 396
MboI GATC 3 cut(s) 42, 219, 394
MboII GAAGA 1 cut(s) 266
MflI RGATCY 1 cut(s) 219
MluCI AATT 6 cut(s) 22, 332, 469, 561, 645, 658
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 2 cut(s) 17, 710
MnlI CCTC 1 cut(s) 535
Mph1103I ATGCAT 1 cut(s) 136
MseI TTAA 7 cut(s) 75, 125, 158, 263, 414, 480, 674
MslI CAYNNNNRTG 2 cut(s) 75, 638
MspA1I CMGCKG 1 cut(s) 377
MspCI CTTAAG 1 cut(s) 479
MwoI GCNNNNNNNGC 1 cut(s) 245
NdeII GATC 3 cut(s) 42, 219, 394
NlaIII CATG 1 cut(s) 688
NlaIV GGNNCC 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 233
NsiI ATGCAT 1 cut(s) 136
NspI RCATGY 1 cut(s) 688
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 3 cut(s) 250, 376, 491
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PshBI ATTAAT 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 103
PstI CTGCAG 1 cut(s) 253
PsuI RGATCY 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 2 cut(s) 75, 638
SaqAI TTAA 7 cut(s) 75, 125, 158, 263, 414, 480, 674
SatI GCNGC 3 cut(s) 249, 375, 490
Sau3AI GATC 3 cut(s) 42, 219, 394
SchI GAGTC 1 cut(s) 83
SfaNI GCATC 2 cut(s) 143, 181
SfcI CTRYAG 2 cut(s) 116, 249
SmiMI CAYNNNNRTG 2 cut(s) 75, 638
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 6 cut(s) 22, 332, 469, 561, 645, 658
SsiI CCGC 3 cut(s) 489, 492, 496
SspI AATATT 1 cut(s) 260
SspMI CTAG 3 cut(s) 198, 309, 742
TaaI ACNGT 2 cut(s) 163, 708
TaiI ACGT 1 cut(s) 414
TaqI TCGA 2 cut(s) 297, 438
TasI AATT 6 cut(s) 22, 332, 469, 561, 645, 658
TauI GCSGC 1 cut(s) 492
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 7 cut(s) 75, 125, 158, 263, 414, 480, 674
Tru9I TTAA 7 cut(s) 75, 125, 158, 263, 414, 480, 674
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 248, 374
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 4 cut(s) 17, 35, 600, 641
Vha464I CTTAAG 1 cut(s) 479
VspI ATTAAT 1 cut(s) 75
XapI RAATTY 4 cut(s) 22, 469, 561, 658
XbaI TCTAGA 1 cut(s) 308
XceI RCATGY 1 cut(s) 688
XspI CTAG 3 cut(s) 198, 309, 742
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.