Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0003239

Physical Location & Seq

Genomic Coordinates Reverse (-)

36612098 .. 36618280

Gene Structure 6183 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G025570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

ATGGAGGCGCCGCCAATTATTTCCCCTACCAAAACGCTGCCGTTCCGATCAGCTTCCCCTTCCTCCTCTCTTCTCTTTCGCCGCAAGCATCGTCTCAATTGCGGCCGCAGACCTTCTAGAAGCTTCACCGTCCGTGCCTCTTCCGCTGCTAGTGATGATTCAGTGGTGACTCTGCTTGAACGGTTGTTCGAATTGCTTTCCAAGTCAACTACTTACTACTACTTTACATTTTCATCAGTCCAAACTCCCAAGGATATTTTGAATGCCAACCGCCTAATCTTTCCTGGTGTGGGGGCTTTCGCTGCGGCCATGGATGTGCTGAGCAAGAATGGGATGGCTGAAGCTCTCTGTGAATATATTGAGAAAGATCGGCCGTTTCTAGGCATTTGTCTTGGACTTCAGCTCCTTTTTGAATCCAGTGAGGAAAAGGGACAAGTGAGTGGTCTTGGCTTGATACCTGGAGTGGTTGGGCGTTTTGACTCATCAAATGGTGTTCGAGTGCCACATATCGGGTGGAATGCTTTGCAGATTAGAGAAGACTCTTTAATTTTGGATGATGTTGGAACCCATCACGTCTATTTCGTCCACTCTTACAGAGCAATGCCATCGGATGATAACAAAGAATGGGTTTCATCTACCTGCAACTATGGTGACAATTTCATTGCATCTGTTAGAAGGGGAAATGTGCATGCAGTTCAATTCCACCCTGAGAAAAGTGGAGATGTTGGTCTTTCCATATTGAGAAGATTTCTCTATCCAAACTCACCCTTGGAGAAGAAGCCCACTGAAGGGAAGGGTTCCAAACTTGCAAAGAGGGTTATTGCTTGTCTGGATGTGAGGACGAATGACAAAGGAGATCTTGTTGTAACCAAAGGAGACCAATATGACGTAAGAGAGCATACAAAAGAGAATGAGGTGAGAGAACTTGGAAAGCCTGTGGAGCTTGCTGGACAGTATTACACAGATGGAGCTGATGAGGTCAGCTTTCTGAATATCACCGGTTTCCGGGACTTCCCTTTGGGTGACTTGCCTATGCTACAGGTATTGAGATATGCATCAGAAAATGTTTTTGTGCCATTAACAGTTGGAGGTGGCATTAGAGATTTTACCGATGCAACTGGCAGGCACTATTCTAGTTTGGAAGTTGCTTCAGAATATTTTAGATGTGGGGCTGATAAGATCTCCATTGGAAGTGATGCAGTATATGCTGCAGAAGAATATTTAAGAACTGGAGTGAAAAGTGGAAAAAGCAGCTTAGAGCAGATATCCAGAGTTTACGGAAATCAGGCTGTGGTTGTAAGCATTGATCCTCGCAGAGTGTATGTTAAAAATCAAAATGATGTAAAGTTCAAGACTATAAGAGCAAAAATCCAGTTTCAAATTTTGTGTGTATTATTGATTTCAGGTCCAAATGGCGAAGAATACGCTTGGTATCAGTGTACAGTTAGCGGTGGGCGGGAAGGCAGACCAATTGGAGCGTATGAGCTTGCAAAAGCAGTTGAGGAGCTTGGAGCTGGAGAAATTCTGCTAAACTGCATTGATTGCGATGGTCAAGGGAAAGGATTTGATATAGATCTAGTAAAGCTCATCTCTGATGCTGTGACCATTCCCGTGATTGCAAGTAGTGGTGCTGGTGGTGTTGAACACTTCTCAGAGGTTTTCATGAAAACAAATGCATCTGCTGCCCTTGCTGCTGGCATATTCCATCGCAAGGAGGTGCCAATTCATTCTGTAAAGGAGCACTTGCTAAAGGAAGGCATAGAAGTCAGAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230

Pfam Domains

Protein Families

GATase His_biosynth

Protein Analysis

591

Amino Acids

64.94

Weight (kDa)

6.53

Isoelectric Point (pI)

38.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	53 - 251	1.7e-20	Glutamine amidotransferase class-I
GATase_3	PF07685	83 - 154	5.2e-08	CobB/CobQ-like glutamine amidotransferase domain
His_biosynth	PF00977	272 - 573	4.8e-40	Histidine biosynthesis protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003239)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26900
fragaria_vesca	FvH4_5g29730 FvH4_5g29730 FvH4_5g29730
malus_domestica	MD03G1198000.v1.1 MD11G1214000.v1.1
prunus_persica	Prupe.4G217200_v2.0.a1
pyrus_communis	pycom03g14950
rosa_chinensis	RchiOBHm_Chr4g0412471 RchiOBHm_Chr5g0040621 RchiOBHm_Chr5g0040671
rosa_laevigata	RLG00000008296 RLG00000033991 RLG00000033993
rosa_multiflora	Rmu_co8258425.1_g000001 Rmu_sc0000057.1_g000007 Rmu_sc0002604.1_g000021 Rmu_sc0003916.1_g000009
rosa_roxburghii	Rroxscaffold_161G00448020 Rroxscaffold_1G00040000 Rroxscaffold_5G00356630
rosa_rugosa	Rorug04G0104000.1 Rorug05G0186200 Rorug05G0186300
rosa_samantha	Rh1DG043400 Rh4AG173300 Rh4BG172600 Rh4CG184200 Rh4DG168700 Rh5AG272700 Rh5BG277100 Rh5BG277700 Rh5CG309600 Rh5DG285600
rosa_wichuraiana	Rw0G015610 Rw1G002220 Rw5G025570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	647
AccB1I	GGYRCC	2 cut(s)	7, 1717
AciI	CCGC	9 cut(s)	11, 82, 102, 106, 144, 271, 305, 1449, 1456
AclWI	GGATC	1 cut(s)	1301
AcoI	YGGCCR	3 cut(s)	103, 306, 371
AcsI	RAATTY	2 cut(s)	1380, 1521
AcuI	CTGAAG	4 cut(s)	360, 383, 807, 1134
AcyI	GRCGYC	1 cut(s)	8
AfaI	GTAC	1 cut(s)	1441
AfiI	CCNNNNNNNGG	7 cut(s)	290, 380, 509, 788, 789, 1005, 1711
AgeI	ACCGGT	1 cut(s)	998
AgsI	TTSAA	7 cut(s)	179, 262, 413, 698, 1351, 1379, 1643
AjiI	CACGTC	1 cut(s)	574
AjnI	CCWGG	2 cut(s)	283, 457
Alw21I	GWGCWC	1 cut(s)	1743
Alw26I	GTCTC	2 cut(s)	98, 870
AlwI	GGATC	1 cut(s)	1301
AlwNI	CAGNNNCTG	1 cut(s)	1773
AoxI	GGCC	3 cut(s)	103, 306, 371
ApeKI	GCWGC	7 cut(s)	37, 146, 302, 1208, 1251, 1682, 1691
ApoI	RAATTY	2 cut(s)	1380, 1521
AsiGI	ACCGGT	1 cut(s)	998
AspLEI	GCGC	1 cut(s)	10
AspS9I	GGNCC	1 cut(s)	1406
AsuC2I	CCSGG	1 cut(s)	1007
AsuHPI	GGTGA	7 cut(s)	118, 178, 662, 756, 928, 988, 1034
AsuII	TTCGAA	1 cut(s)	189
AvaII	GGWCC	1 cut(s)	1406
BanI	GGYRCC	2 cut(s)	7, 1717
BbsI	GAAGAC	1 cut(s)	543
Bbv12I	GWGCWC	1 cut(s)	1743
BbvI	GCAGC	7 cut(s)	24, 133, 289, 1195, 1263, 1669, 1678
BccI	CCATC	6 cut(s)	328, 576, 613, 959, 1541, 1713
BceAI	ACGGC	2 cut(s)	25, 358
BcgI	CGANNNNNNTGC	2 cut(s)	588, 622
BciT130I	CCWGG	2 cut(s)	285, 459
BcnI	CCSGG	1 cut(s)	1007
BcoDI	GTCTC	2 cut(s)	98, 870
BfaI	CTAG	5 cut(s)	117, 150, 380, 1134, 1577
BfmI	CTRYAG	2 cut(s)	1037, 1209
BfoI	RGCGCY	1 cut(s)	11
BfuAI	ACCTGC	1 cut(s)	647
BglII	AGATCT	3 cut(s)	856, 1179, 1573
BlpI	GCTNAGC	1 cut(s)	320
Bme1390I	CCNGG	3 cut(s)	285, 459, 1007
Bme18I	GGWCC	1 cut(s)	1406
BmgBI	CACGTC	1 cut(s)	574
BmgT120I	GGNCC	1 cut(s)	1406
BmiI	GGNNCC	4 cut(s)	9, 565, 799, 1719
BmrFI	CCNGG	3 cut(s)	285, 459, 1007
BmsI	GCATC	7 cut(s)	97, 674, 1064, 1102, 1186, 1585, 1685
BpiI	GAAGAC	1 cut(s)	543
BpmI	CTGGAG	3 cut(s)	480, 1251, 1536
Bpu1102I	GCTNAGC	1 cut(s)	320
Bpu14I	TTCGAA	1 cut(s)	189
BpuMI	CCSGG	1 cut(s)	1007
BsaBI	GATNNNNATC	2 cut(s)	1054, 1572
BsaHI	GRCGYC	1 cut(s)	8
BsaI	GGTCTC	1 cut(s)	870
BsaJI	CCNNGG	3 cut(s)	249, 309, 768
BsaWI	WCCGGW	1 cut(s)	998
BsaXI	ACNNNNNCTCC	4 cut(s)	387, 417, 846, 876
Bsc4I	CCNNNNNNNGG	7 cut(s)	290, 380, 509, 788, 789, 1005, 1711
Bse118I	RCCGGY	1 cut(s)	998
Bse1I	ACTGG	4 cut(s)	417, 1123, 1234, 1372
Bse3DI	GCAATG	2 cut(s)	606, 660
Bse8I	GATNNNNATC	2 cut(s)	1054, 1572
BseBI	CCWGG	2 cut(s)	285, 459
BseDI	CCNNGG	3 cut(s)	249, 309, 768
BseGI	GGATG	5 cut(s)	319, 339, 559, 616, 838
BseJI	GATNNNNATC	2 cut(s)	1054, 1572
BseLI	CCNNNNNNNGG	7 cut(s)	290, 380, 509, 788, 789, 1005, 1711
BseMI	GCAATG	2 cut(s)	606, 660
BseMII	CTCAG	3 cut(s)	311, 699, 1665
BseNI	ACTGG	4 cut(s)	417, 1123, 1234, 1372
BseRI	GAGGAG	2 cut(s)	55, 1517
BseX3I	CGGCCG	2 cut(s)	103, 371
BseXI	GCAGC	7 cut(s)	24, 133, 289, 1195, 1263, 1669, 1678
Bsh1285I	CGRYCG	2 cut(s)	106, 374
BshFI	GGCC	3 cut(s)	105, 308, 373
BshNI	GGYRCC	2 cut(s)	7, 1717
BshTI	ACCGGT	1 cut(s)	998
BsiEI	CGRYCG	2 cut(s)	106, 374
BsiHKAI	GWGCWC	1 cut(s)	1743
BsiSI	CCGG	2 cut(s)	999, 1006
BslFI	GGGAC	2 cut(s)	444, 1022
BslI	CCNNNNNNNGG	7 cut(s)	290, 380, 509, 788, 789, 1005, 1711
BsmAI	GTCTC	2 cut(s)	98, 870
BsmBI	CGTCTC	1 cut(s)	98
BsmFI	GGGAC	2 cut(s)	444, 1022
BsmI	GAATGC	2 cut(s)	268, 523
BsnI	GGCC	3 cut(s)	105, 308, 373
Bso31I	GGTCTC	1 cut(s)	870
Bsp119I	TTCGAA	1 cut(s)	189
Bsp1286I	GDGCHC	1 cut(s)	1743
Bsp1407I	TGTACA	1 cut(s)	1439
Bsp143I	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
Bsp1720I	GCTNAGC	1 cut(s)	320
Bsp19I	CCATGG	1 cut(s)	309
BspACI	CCGC	9 cut(s)	11, 82, 102, 106, 144, 271, 305, 1449, 1456
BspANI	GGCC	3 cut(s)	105, 308, 373
BspCNI	CTCAG	3 cut(s)	312, 700, 1664
BspHI	TCATGA	1 cut(s)	1662
BspLI	GGNNCC	4 cut(s)	9, 565, 799, 1719
BspMAI	CTGCAG	1 cut(s)	1213
BspMI	ACCTGC	1 cut(s)	647
BspPI	GGATC	1 cut(s)	1301
BspT104I	TTCGAA	1 cut(s)	189
BspT107I	GGYRCC	2 cut(s)	7, 1717
BspTNI	GGTCTC	1 cut(s)	870
BsrDI	GCAATG	2 cut(s)	606, 660
BsrFI	RCCGGY	1 cut(s)	998
BsrGI	TGTACA	1 cut(s)	1439
BsrI	ACTGG	4 cut(s)	417, 1123, 1234, 1372
BssAI	RCCGGY	1 cut(s)	998
BssECI	CCNNGG	3 cut(s)	249, 309, 768
BssMI	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
BssNI	GRCGYC	1 cut(s)	8
BssT1I	CCWWGG	3 cut(s)	249, 309, 768
Bst2UI	CCWGG	2 cut(s)	285, 459
Bst4CI	ACNGT	5 cut(s)	130, 183, 954, 1084, 1444
Bst6I	CTCTTC	2 cut(s)	75, 145
BstACI	GRCGYC	1 cut(s)	8
BstAPI	GCANNNNNTGC	3 cut(s)	1205, 1542, 1682
BstAUI	TGTACA	1 cut(s)	1439
BstBI	TTCGAA	1 cut(s)	189
BstC8I	GCNNGC	6 cut(s)	86, 690, 945, 1124, 1488, 1696
BstDEI	CTNAG	4 cut(s)	320, 708, 1255, 1651
BstDSI	CCRYGG	1 cut(s)	309
BstF5I	GGATG	5 cut(s)	319, 339, 559, 616, 838
BstH2I	RGCGCY	1 cut(s)	11
BstHHI	GCGC	1 cut(s)	10
BstKTI	GATC	6 cut(s)	50, 370, 859, 1182, 1309, 1576
BstMAI	GTCTC	2 cut(s)	98, 870
BstMBI	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
BstMCI	CGRYCG	2 cut(s)	106, 374
BstMWI	GCNNNNNNNGC	8 cut(s)	143, 302, 940, 1205, 1542, 1682, 1688, 1691
BstNI	CCWGG	2 cut(s)	285, 459
BstNSI	RCATGY	1 cut(s)	692
BstSCI	CCNGG	3 cut(s)	283, 457, 1005
BstSFI	CTRYAG	2 cut(s)	1037, 1209
BstV1I	GCAGC	7 cut(s)	24, 133, 289, 1195, 1263, 1669, 1678
BstV2I	GAAGAC	1 cut(s)	543
BstX2I	RGATCY	3 cut(s)	856, 1179, 1573
BstYI	RGATCY	3 cut(s)	856, 1179, 1573
BstZI	CGGCCG	2 cut(s)	103, 371
BsuRI	GGCC	3 cut(s)	105, 308, 373
BtgI	CCRYGG	1 cut(s)	309
BtgZI	GCGATG	2 cut(s)	1560, 1691
BtrI	CACGTC	1 cut(s)	574
BtsCI	GGATG	5 cut(s)	319, 339, 559, 616, 838
BtsIMutI	CAGTG	4 cut(s)	168, 424, 783, 1442
BveI	ACCTGC	1 cut(s)	647
Cac8I	GCNNGC	6 cut(s)	86, 690, 945, 1124, 1488, 1696
CaiI	CAGNNNCTG	1 cut(s)	1773
CciI	TCATGA	1 cut(s)	1662
CciNI	GCGGCCGC	1 cut(s)	103
CfoI	GCGC	1 cut(s)	10
Cfr10I	RCCGGY	1 cut(s)	998
Cfr13I	GGNCC	1 cut(s)	1406
Csp6I	GTAC	1 cut(s)	1440
CspAI	ACCGGT	1 cut(s)	998
CviAII	CATG	3 cut(s)	310, 689, 1663
CviQI	GTAC	1 cut(s)	1440
DdeI	CTNAG	4 cut(s)	320, 708, 1255, 1651
DinI	GGCGCC	1 cut(s)	9
DpnI	GATC	6 cut(s)	49, 369, 858, 1181, 1308, 1575
DpnII	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
EaeI	YGGCCR	3 cut(s)	103, 306, 371
EagI	CGGCCG	2 cut(s)	103, 371
Eam1104I	CTCTTC	2 cut(s)	75, 145
EarI	CTCTTC	2 cut(s)	75, 145
EclXI	CGGCCG	2 cut(s)	103, 371
Eco130I	CCWWGG	3 cut(s)	249, 309, 768
Eco31I	GGTCTC	1 cut(s)	870
Eco32I	GATATC	1 cut(s)	1266
Eco47I	GGWCC	1 cut(s)	1406
Eco52I	CGGCCG	2 cut(s)	103, 371
Eco57I	CTGAAG	4 cut(s)	360, 383, 807, 1134
EcoRII	CCWGG	2 cut(s)	283, 457
EcoRV	GATATC	1 cut(s)	1266
EcoT14I	CCWWGG	3 cut(s)	249, 309, 768
EcoT22I	ATGCAT	2 cut(s)	1057, 1678
EgeI	GGCGCC	1 cut(s)	9
EheI	GGCGCC	1 cut(s)	9
ErhI	CCWWGG	3 cut(s)	249, 309, 768
Esp3I	CGTCTC	1 cut(s)	98
FaeI	CATG	3 cut(s)	313, 692, 1666
FalI	AAGNNNNNCTT	4 cut(s)	843, 875, 1727, 1759
FaqI	GGGAC	2 cut(s)	444, 1022
FatI	CATG	3 cut(s)	309, 688, 1662
FauI	CCCGC	1 cut(s)	1449
FokI	GGATG	5 cut(s)	326, 346, 566, 623, 845
FspBI	CTAG	5 cut(s)	117, 150, 380, 1134, 1577
GlaI	GCGC	1 cut(s)	9
GsuI	CTGGAG	3 cut(s)	480, 1251, 1536
HaeII	RGCGCY	1 cut(s)	11
HaeIII	GGCC	3 cut(s)	105, 308, 373
HapII	CCGG	2 cut(s)	999, 1006
HhaI	GCGC	1 cut(s)	10
Hin1I	GRCGYC	1 cut(s)	8
Hin1II	CATG	3 cut(s)	313, 692, 1666
Hin6I	GCGC	1 cut(s)	8
HinP1I	GCGC	1 cut(s)	8
HincII	GTYRAC	1 cut(s)	207
HindII	GTYRAC	1 cut(s)	207
HindIII	AAGCTT	1 cut(s)	121
HinfI	GANTC	6 cut(s)	158, 169, 413, 479, 539, 1770
HpaII	CCGG	2 cut(s)	999, 1006
HphI	GGTGA	7 cut(s)	118, 178, 662, 756, 928, 988, 1034
Hpy166II	GTNNAC	4 cut(s)	207, 586, 1276, 1440
Hpy188I	TCNGA	9 cut(s)	47, 610, 990, 1060, 1153, 1594, 1654, 1769, 1775
Hpy188III	TCNNGA	5 cut(s)	117, 830, 1269, 1351, 1663
Hpy8I	GTNNAC	4 cut(s)	207, 586, 1276, 1440
HpyAV	CCTTC	7 cut(s)	69, 123, 669, 782, 787, 1454, 1748
HpyCH4III	ACNGT	5 cut(s)	130, 183, 954, 1084, 1444
HpyCH4IV	ACGT	2 cut(s)	573, 888
HpyF10VI	GCNNNNNNNGC	8 cut(s)	143, 302, 940, 1205, 1542, 1682, 1688, 1691
HpyF3I	CTNAG	4 cut(s)	320, 708, 1255, 1651
HpySE526I	ACGT	2 cut(s)	573, 888
Hsp92I	GRCGYC	1 cut(s)	8
Hsp92II	CATG	3 cut(s)	313, 692, 1666
HspAI	GCGC	1 cut(s)	8
KasI	GGCGCC	1 cut(s)	7
Kzo9I	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
LmnI	GCTCC	7 cut(s)	408, 940, 968, 1475, 1504, 1511, 1738
Lsp1109I	GCAGC	7 cut(s)	24, 133, 289, 1195, 1263, 1669, 1678
LweI	GCATC	7 cut(s)	97, 674, 1064, 1102, 1186, 1585, 1685
MaeI	CTAG	5 cut(s)	117, 150, 380, 1134, 1577
MaeII	ACGT	2 cut(s)	573, 888
MaeIII	GTNAC	5 cut(s)	166, 650, 865, 1022, 1600
MalI	GATC	6 cut(s)	49, 369, 858, 1181, 1308, 1575
MboI	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
MboII	GAAGA	7 cut(s)	62, 132, 548, 756, 787, 1226, 1430
MfeI	CAATTG	2 cut(s)	97, 1470
MflI	RGATCY	3 cut(s)	856, 1179, 1573
MhlI	GDGCHC	1 cut(s)	1743
Mly113I	GGCGCC	1 cut(s)	8
MlyI	GAGTC	3 cut(s)	163, 473, 533
MmeI	TCCRAC	2 cut(s)	541, 1066
Mph1103I	ATGCAT	2 cut(s)	1057, 1678
MseI	TTAA	4 cut(s)	545, 1079, 1223, 1326
MslI	CAYNNNNRTG	2 cut(s)	314, 1610
MspA1I	CMGCKG	1 cut(s)	146
MspI	CCGG	2 cut(s)	999, 1006
MspR9I	CCNGG	3 cut(s)	285, 459, 1007
MunI	CAATTG	2 cut(s)	97, 1470
Mva1269I	GAATGC	2 cut(s)	268, 523
MvaI	CCWGG	2 cut(s)	285, 459
MwoI	GCNNNNNNNGC	8 cut(s)	143, 302, 940, 1205, 1542, 1682, 1688, 1691
NarI	GGCGCC	1 cut(s)	8
NciI	CCSGG	1 cut(s)	1007
NcoI	CCATGG	1 cut(s)	309
NdeII	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
NlaIII	CATG	3 cut(s)	313, 692, 1666
NlaIV	GGNNCC	4 cut(s)	9, 565, 799, 1719
NmuCI	GTSAC	4 cut(s)	166, 650, 1022, 1600
NotI	GCGGCCGC	1 cut(s)	103
NsiI	ATGCAT	2 cut(s)	1057, 1678
NspI	RCATGY	1 cut(s)	692
NspV	TTCGAA	1 cut(s)	189
PaeI	GCATGC	1 cut(s)	692
PagI	TCATGA	1 cut(s)	1662
PcsI	WCGNNNNNNNCGW	1 cut(s)	579
PctI	GAATGC	2 cut(s)	268, 523
PfeI	GAWTC	3 cut(s)	158, 413, 1770
PfoI	TCCNGGA	1 cut(s)	1005
PinAI	ACCGGT	1 cut(s)	998
PleI	GAGTC	3 cut(s)	163, 473, 533
PluTI	GGCGCC	1 cut(s)	11
PpsI	GAGTC	3 cut(s)	163, 473, 533
Psp6I	CCWGG	2 cut(s)	283, 457
PspGI	CCWGG	2 cut(s)	283, 457
PspN4I	GGNNCC	4 cut(s)	9, 565, 799, 1719
PspPI	GGNCC	1 cut(s)	1406
PstI	CTGCAG	1 cut(s)	1213
PstNI	CAGNNNCTG	1 cut(s)	1773
PsuI	RGATCY	3 cut(s)	856, 1179, 1573
RsaI	GTAC	1 cut(s)	1441
RsaNI	GTAC	1 cut(s)	1440
RseI	CAYNNNNRTG	2 cut(s)	314, 1610
SaqAI	TTAA	4 cut(s)	545, 1079, 1223, 1326
Sau3AI	GATC	6 cut(s)	47, 367, 856, 1179, 1306, 1573
Sau96I	GGNCC	1 cut(s)	1406
SchI	GAGTC	3 cut(s)	163, 473, 533
ScrFI	CCNGG	3 cut(s)	285, 459, 1007
SduI	GDGCHC	1 cut(s)	1743
SfaNI	GCATC	7 cut(s)	97, 674, 1064, 1102, 1186, 1585, 1685
SfcI	CTRYAG	2 cut(s)	1037, 1209
SfoI	GGCGCC	1 cut(s)	9
SfuI	TTCGAA	1 cut(s)	189
SinI	GGWCC	1 cut(s)	1406
SmiMI	CAYNNNNRTG	2 cut(s)	314, 1610
SphI	GCATGC	1 cut(s)	692
SsiI	CCGC	9 cut(s)	11, 82, 102, 106, 144, 271, 305, 1449, 1456
SspDI	GGCGCC	1 cut(s)	7
SspI	AATATT	2 cut(s)	1157, 1220
SspMI	CTAG	5 cut(s)	117, 150, 380, 1134, 1577
StyD4I	CCNGG	3 cut(s)	283, 457, 1005
StyI	CCWWGG	3 cut(s)	249, 309, 768
TaaI	ACNGT	5 cut(s)	130, 183, 954, 1084, 1444
TaiI	ACGT	2 cut(s)	576, 891
TaqI	TCGA	2 cut(s)	189, 496
TatI	WGTACW	1 cut(s)	1439
TauI	GCSGC	5 cut(s)	13, 84, 105, 108, 308
TfiI	GAWTC	3 cut(s)	158, 413, 1770
Tru1I	TTAA	4 cut(s)	545, 1079, 1223, 1326
Tru9I	TTAA	4 cut(s)	545, 1079, 1223, 1326
TscAI	CASTG	4 cut(s)	168, 424, 790, 1442
TseFI	GTSAC	4 cut(s)	166, 650, 1022, 1600
TseI	GCWGC	7 cut(s)	37, 146, 302, 1208, 1251, 1682, 1691
Tsp45I	GTSAC	4 cut(s)	166, 650, 1022, 1600
TspDTI	ATGAA	6 cut(s)	222, 621, 649, 1651, 1679, 1715
TspGWI	ACGGA	2 cut(s)	122, 1293
TspRI	CASTG	4 cut(s)	168, 424, 790, 1442
VpaK11BI	GGWCC	1 cut(s)	1406
XapI	RAATTY	2 cut(s)	1380, 1521
XbaI	TCTAGA	1 cut(s)	116
XceI	RCATGY	1 cut(s)	692
XcmI	CCANNNNNNNNNTGG	1 cut(s)	510
XspI	CTAG	5 cut(s)	117, 150, 380, 1134, 1577
Zsp2I	ATGCAT	2 cut(s)	1057, 1678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.