MD11G1214000.v1.1

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003239
Physical Location & Seq
Forward (+)
31266277 .. 31270782
4506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1214000.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGTGCTTTAGATATGTTGGTCTTTCGATATTGAGAAGATTTTTGTATCCAAGGGCAAATCTGGAAAGGAAGCCCACTGAAAGGAAGGCTTTGAAACTTGCTAAGAGGGTGATTGCTTGTCTTGATGTGAGGACGAATGACAGGGGAGATCTTGTTGTAACCAAAGGTGACCAATATGATGTAAGAGAGCACTCAAAAGAGAATGAGGCGAGAGAACTTGGTAAGCCTGTGGAGCTTGCTCGACAATATTACAAAGATGGGGCTGATGAGGTCAGCTTTCTAAATATTACTGGTTTTCGAGACTTCCCTCTGGGCGACTTGCCCATGTTACAGGTATTGAGATATACATCAGAAAATGTTTTTGTACCATTAACAGTCGGAGGTGGCATCAGAGATTTTACAGATGCAAATGGCAGGCACTATTCTAGTTTAGAAGTTGCTTCGGAATATTTCAGATGTGGGGCTGATAAGATTTCTATTGGAAGCGATGCAGTTTATGCTGCAGAAGAATATTTGAGAACTGGAGTAAAAAGTGGAAAAAGTAGCTTAGAGCAGATATCTAGAGTTTATGGAAATCAGGCTGTGGTCGTGAGCATTGATCCTTGCAGAGTGTATGTTAAAAATCCAAAAGATGTAGAGTTCAAGACTATAAGGGTAGCAACCCAAGGTCCAAATGGAGAAGAATTTGCATGGTACCAGTGCACAGTTAGCGGTGGCCGAGAAGGCCGACCAATTGGAGCGTATGAGCTTGCAAAAGCAGTTGAGGAGCTTGGAGCTGGAGAAATACTGCTAAACTGCATTGATTGTGACGGTCAAAAGAAGGGATTTGATATAGATTTAATAAAGCTGATCTCAAACGCTGTGAACATTCCTGTGATTGCTAGTAGCGGTGCTGGTTCTGCTGAACACTTCTCCGAGGTGTTCAGGAAAACAAATGCATCGGCTGCCCTAGCTGCCGGCATTTTTCATCGGAAGGAGGTGCCTATTCAGTCAGTGAAGGAACACTTGTTAAACGAACGCATAGAAGTCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

346

Amino Acids

38.31

Weight (kDa)

7.56

Isoelectric Point (pI)

27.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 36 - 327 6e-44 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 693
AccB1I GGYRCC 2 cut(s) 693, 979
AciI CCGC 2 cut(s) 711, 888
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 1 cut(s) 715
AcsI RAATTY 1 cut(s) 683
AfaI GTAC 2 cut(s) 366, 695
AfiI CCNNNNNNNGG 1 cut(s) 81
AgsI TTSAA 2 cut(s) 94, 643
AluBI AGCT 8 cut(s) 235, 276, 546, 748, 769, 776, 847, 953
AluI AGCT 8 cut(s) 235, 276, 546, 748, 769, 776, 847, 953
Alw21I GWGCWC 2 cut(s) 192, 704
Alw26I GTCTC 1 cut(s) 294
Alw44I GTGCAC 1 cut(s) 700
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 1 cut(s) 1035
AoxI GGCC 2 cut(s) 715, 724
ApaLI GTGCAC 1 cut(s) 700
ApeKI GCWGC 3 cut(s) 500, 944, 953
ApoI RAATTY 1 cut(s) 683
Asp718I GGTACC 1 cut(s) 693
AspS9I GGNCC 1 cut(s) 668
AsuHPI GGTGA 2 cut(s) 121, 179
AvaII GGWCC 1 cut(s) 668
BaeGI GKGCMC 1 cut(s) 704
BanI GGYRCC 2 cut(s) 693, 979
Bbv12I GWGCWC 2 cut(s) 192, 704
BbvI GCAGC 3 cut(s) 487, 931, 940
BccI CCATC 1 cut(s) 251
BciVI GTATCC 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 4 cut(s) 426, 561, 882, 950
BfmI CTRYAG 1 cut(s) 501
BfuI GTATCC 1 cut(s) 57
BglI GCCNNNNNGGC 1 cut(s) 723
BglII AGATCT 1 cut(s) 148
BisI GCNGC 3 cut(s) 501, 945, 954
BlsI GCNGC 3 cut(s) 502, 946, 955
Bme18I GGWCC 1 cut(s) 668
BmgT120I GGNCC 1 cut(s) 668
BmiI GGNNCC 2 cut(s) 695, 981
BmsI GCATC 4 cut(s) 394, 396, 478, 947
BpmI CTGGAG 2 cut(s) 543, 798
BsaBI GATNNNNATC 1 cut(s) 346
BsaJI CCNNGG 3 cut(s) 50, 664, 915
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 1 cut(s) 81
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 3 cut(s) 295, 526, 697
Bse8I GATNNNNATC 1 cut(s) 346
BseDI CCNNGG 3 cut(s) 50, 664, 915
BseJI GATNNNNATC 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 81
BseNI ACTGG 3 cut(s) 295, 526, 697
BseRI GAGGAG 1 cut(s) 779
BseSI GKGCMC 1 cut(s) 704
BseXI GCAGC 3 cut(s) 487, 931, 940
BshFI GGCC 2 cut(s) 717, 726
BshNI GGYRCC 2 cut(s) 693, 979
BsiHKAI GWGCWC 2 cut(s) 192, 704
BsiSI CCGG 1 cut(s) 957
BslI CCNNNNNNNGG 1 cut(s) 81
BsmAI GTCTC 1 cut(s) 294
BsnI GGCC 2 cut(s) 717, 726
Bsp1286I GDGCHC 2 cut(s) 192, 704
Bsp143I GATC 3 cut(s) 148, 598, 849
BspACI CCGC 2 cut(s) 711, 888
BspANI GGCC 2 cut(s) 717, 726
BspLI GGNNCC 2 cut(s) 695, 981
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 1 cut(s) 593
BspT107I GGYRCC 2 cut(s) 693, 979
BsrFI RCCGGY 1 cut(s) 956
BsrI ACTGG 3 cut(s) 295, 526, 697
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 3 cut(s) 50, 664, 915
BssMI GATC 3 cut(s) 148, 598, 849
BssT1I CCWWGG 2 cut(s) 50, 664
Bst4CI ACNGT 3 cut(s) 376, 706, 812
BstAPI GCANNNNNTGC 2 cut(s) 497, 944
BstC8I GCNNGC 4 cut(s) 237, 416, 750, 958
BstDEI CTNAG 2 cut(s) 102, 547
BstEII GGTNACC 1 cut(s) 167
BstKTI GATC 3 cut(s) 151, 601, 852
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 3 cut(s) 148, 598, 849
BstMWI GCNNNNNNNGC 8 cut(s) 232, 497, 708, 723, 899, 944, 950, 953
BstPI GGTNACC 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 704
BstV1I GCAGC 3 cut(s) 487, 931, 940
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
BsuI GTATCC 1 cut(s) 57
BsuRI GGCC 2 cut(s) 717, 726
BtgZI GCGATG 1 cut(s) 501
BtsIMutI CAGTG 3 cut(s) 75, 704, 999
Cac8I GCNNGC 4 cut(s) 237, 416, 750, 958
CaiI CAGNNNCTG 1 cut(s) 1035
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 1 cut(s) 668
Csp6I GTAC 2 cut(s) 365, 694
CviAII CATG 2 cut(s) 325, 690
CviQI GTAC 2 cut(s) 365, 694
DdeI CTNAG 2 cut(s) 102, 547
DpnI GATC 3 cut(s) 150, 600, 851
DpnII GATC 3 cut(s) 148, 598, 849
EaeI YGGCCR 1 cut(s) 715
Eco130I CCWWGG 2 cut(s) 50, 664
Eco32I GATATC 1 cut(s) 558
Eco47I GGWCC 1 cut(s) 668
Eco91I GGTNACC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 167
EcoRV GATATC 1 cut(s) 558
EcoT14I CCWWGG 2 cut(s) 50, 664
EcoT22I ATGCAT 1 cut(s) 940
ErhI CCWWGG 2 cut(s) 50, 664
FaeI CATG 2 cut(s) 328, 693
FalI AAGNNNNNCTT 4 cut(s) 73, 105, 989, 1021
FatI CATG 2 cut(s) 324, 689
Fnu4HI GCNGC 3 cut(s) 501, 945, 954
Fsp4HI GCNGC 3 cut(s) 501, 945, 954
FspBI CTAG 4 cut(s) 426, 561, 882, 950
GluI GCNGC 3 cut(s) 501, 945, 954
GsuI CTGGAG 2 cut(s) 543, 798
HaeIII GGCC 2 cut(s) 717, 726
HapII CCGG 1 cut(s) 957
Hin1II CATG 2 cut(s) 328, 693
HinfI GANTC 1 cut(s) 1032
HpaII CCGG 1 cut(s) 957
HphI GGTGA 2 cut(s) 121, 179
Hpy166II GTNNAC 2 cut(s) 702, 865
Hpy188I TCNGA 9 cut(s) 352, 380, 392, 445, 455, 916, 972, 1031, 1037
Hpy188III TCNNGA 7 cut(s) 62, 122, 299, 561, 589, 643, 925
Hpy8I GTNNAC 2 cut(s) 702, 865
HpyAV CCTTC 5 cut(s) 79, 716, 814, 967, 991
HpyCH4III ACNGT 3 cut(s) 376, 706, 812
HpyCH4V TGCA 9 cut(s) 407, 491, 503, 606, 689, 702, 752, 798, 938
HpyF10VI GCNNNNNNNGC 8 cut(s) 232, 497, 708, 723, 899, 944, 950, 953
HpyF3I CTNAG 2 cut(s) 102, 547
Hsp92II CATG 2 cut(s) 328, 693
KpnI GGTACC 1 cut(s) 697
KroI GCCGGC 1 cut(s) 956
KroNI GCCGGC 1 cut(s) 958
Kzo9I GATC 3 cut(s) 148, 598, 849
LmnI GCTCC 4 cut(s) 232, 737, 766, 773
Lsp1109I GCAGC 3 cut(s) 487, 931, 940
LweI GCATC 4 cut(s) 394, 396, 478, 947
MaeI CTAG 4 cut(s) 426, 561, 882, 950
MaeIII GTNAC 4 cut(s) 157, 167, 327, 806
MalI GATC 3 cut(s) 150, 600, 851
MboI GATC 3 cut(s) 148, 598, 849
MboII GAAGA 3 cut(s) 48, 518, 692
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 2 cut(s) 192, 704
MluCI AATT 2 cut(s) 683, 732
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 9 cut(s) 99, 123, 199, 262, 318, 374, 757, 910, 970
Mph1103I ATGCAT 1 cut(s) 940
MroNI GCCGGC 1 cut(s) 956
MseI TTAA 4 cut(s) 371, 618, 839, 1010
MslI CAYNNNNRTG 1 cut(s) 872
MspI CCGG 1 cut(s) 957
MunI CAATTG 1 cut(s) 732
MwoI GCNNNNNNNGC 8 cut(s) 232, 497, 708, 723, 899, 944, 950, 953
NaeI GCCGGC 1 cut(s) 958
NdeII GATC 3 cut(s) 148, 598, 849
NgoMIV GCCGGC 1 cut(s) 956
NlaIII CATG 2 cut(s) 328, 693
NlaIV GGNNCC 2 cut(s) 695, 981
NmeAIII GCCGAG 1 cut(s) 743
NmuCI GTSAC 2 cut(s) 167, 806
NsiI ATGCAT 1 cut(s) 940
PdiI GCCGGC 1 cut(s) 958
PfeI GAWTC 1 cut(s) 1032
PkrI GCNGC 3 cut(s) 502, 946, 955
PspEI GGTNACC 1 cut(s) 167
PspN4I GGNNCC 2 cut(s) 695, 981
PspPI GGNCC 1 cut(s) 668
PstI CTGCAG 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 1035
PsuI RGATCY 1 cut(s) 148
RsaI GTAC 2 cut(s) 366, 695
RsaNI GTAC 2 cut(s) 365, 694
RseI CAYNNNNRTG 1 cut(s) 872
SaqAI TTAA 4 cut(s) 371, 618, 839, 1010
SatI GCNGC 3 cut(s) 501, 945, 954
Sau3AI GATC 3 cut(s) 148, 598, 849
Sau96I GGNCC 1 cut(s) 668
SduI GDGCHC 2 cut(s) 192, 704
SfaNI GCATC 4 cut(s) 394, 396, 478, 947
SfcI CTRYAG 1 cut(s) 501
SfiI GGCCNNNNNGGCC 1 cut(s) 723
SinI GGWCC 1 cut(s) 668
SmiMI CAYNNNNRTG 1 cut(s) 872
Sse9I AATT 2 cut(s) 683, 732
SsiI CCGC 2 cut(s) 711, 888
SspI AATATT 4 cut(s) 248, 286, 449, 512
SspMI CTAG 4 cut(s) 426, 561, 882, 950
StyI CCWWGG 2 cut(s) 50, 664
TaaI ACNGT 3 cut(s) 376, 706, 812
TaqI TCGA 3 cut(s) 26, 241, 298
TasI AATT 2 cut(s) 683, 732
TfiI GAWTC 1 cut(s) 1032
Tru1I TTAA 4 cut(s) 371, 618, 839, 1010
Tru9I TTAA 4 cut(s) 371, 618, 839, 1010
TscAI CASTG 3 cut(s) 82, 704, 999
TseFI GTSAC 2 cut(s) 167, 806
TseI GCWGC 3 cut(s) 500, 944, 953
Tsp45I GTSAC 2 cut(s) 167, 806
TspDTI ATGAA 1 cut(s) 956
TspRI CASTG 3 cut(s) 82, 704, 999
VneI GTGCAC 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 668
XapI RAATTY 1 cut(s) 683
XbaI TCTAGA 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XspI CTAG 4 cut(s) 426, 561, 882, 950
Zsp2I ATGCAT 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.