MD03G1198000.v1.1

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003239
Physical Location & Seq
Forward (+)
27096167 .. 27103021
6855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1198000.v1.1.491

Sequence Viewer

Length: 1740 bp
ATGGAGGCGCCGCCATTCCCCTCTTCCTCCAAAACCCTACCGTTTCGCTCATCCTCCGCTTCCTCCCTTCTGTTTCTTCCCAAAACTCACCTCAATTTCAGACGTCCCAGAAGCTTCTCCGTCCGTGCCTCCTCCGCCGCAGATTCCGGGGTGACTCTGCTCGACTACGGCGCCGGCAATGTTCGCAGTGTGAGGAATGCCATTCGCCACCTAGGCTTCGACGTCAAAGATGTCCAAACACCGGAAGACATTCTGAATGCCGAACGCCTAGTATTTCCTGGCGTGGGGGCTTTCGCTGCCGCCATGGATGTGCTGAATAAGAACGGGATGGCGGAAGCAATCTGTTCATATATTGAGAAGGATCGACCATTTCTTGGCATTTGTCTTGGACTTCAGCTCCTTTTTGAATCCAGCGAAGAGAATGGACCGGTGAAAGGTCTTGGCTTGATACCTGGAGTGGTTGGCCGTTTTGATTCATCAAATGGTTTCAGAGTTCCACACATTGGATGGAATGCTTTGCAGATTGAAAAAGACTCATTAATTTTGGATGATGTTGGAAGCCATCATGTCTATTTTGTTCATTCTTACAGAGCCATGCCTTCAGATGAAAACAAAGACTGGGTTTCGTCTACTTGCAACTATGGAGACAATTTTATTGCATCTGTTAGAAGGGGAAATGTGCATGCAGTTCAATTCCACCCAGAAAAGAGTGGAGATGTTGGTCTTACGATATTGAGAAGATTTTTGTATCCAAAGACAAACCTGGAAAAGAAGCCAACTGAAAGGAAGGCTTTGAAACTTGCTAAGAGGGTGATTGCTTGTCTTGATGTGAGGACGAATGACAGGGGAGATCTTGTTGTAACCAAAGGCGACCAATATGATGTAAGAGAGCACACAAAAGAGAATGAGGTGAGAGAACTTGGTAAGCCTGTAGAGCTTGCTCGACAGTATTACAAAGATGGGGCTGATGAGGTCAGCTTTCTGAATATTACTGGTTTTCGAGACTTCCCTCTGGGCGACTTGCCCATGTTACAGGTATTGAGATACACATCAGAAAATGTTTTTGTACCATTAACAGTTGGAGGTGGCATCAGAGATTTTACAGATGCAAATGGCAGGCACTATTCTAGTTTGGAAGTTGCTTCCGAATATTTCAGATGTGGGGCTGATAAGATTTCTATTGGAAGCGATGCAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGTAAAAAATGGAAAAAGTAGCTTAGAGCAGATATCTAGAGTTTATGGAAATCAGGCTGTGGTTGTGAGCATTGATCCTCGCAGAGTGTACATTAAGAATCCAAAAGATGTAGAGTTCAAGACTATAAGGGTAGCAAACCCAGGTCCAAATGGAGAAGAATTTGCATGGTACCAGTGCACAGTTAGCGGTGGCCGAGAAGGCCGACCAATTGGGGCGTATGAGCTTGCAAAAGCAGTAGAGGAGCTCGGAGCTGGAGAAATACTGCTAAACTGCATTGATTGTGATGGTCAAAAGAAGGGATTTGATATAGATTTAATAAAGCTGATCTCAGATGCTGTGAGCATTCCTGTGATTGCTAGTAGCGGTGCTGGTTCTGCCGAACACTTCTCAGAGGTGTTCAGGAAAACAAATGCATCGGCTGCCCTAGCTGCTGGTATTTTCCATCGGAAGGAGGTGCCTATTCAGTCAGTGAAGGAGCACTTGTTAAACGAACAAATAGAAGTCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

580

Amino Acids

63.75

Weight (kDa)

6.53

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 53 - 249 9.3e-24 Glutamine amidotransferase class-I
SNO PF01174 59 - 239 1.1e-06 SNO glutamine amidotransferase family
GATase_3 PF07685 75 - 152 2e-07 CobB/CobQ-like glutamine amidotransferase domain
His_biosynth PF00977 270 - 561 7.5e-44 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 106, 225
Acc65I GGTACC 1 cut(s) 1395
AccB1I GGYRCC 4 cut(s) 7, 170, 1395, 1681
AccB7I CCANNNNNTGG 2 cut(s) 374, 503
AccI GTMKAC 1 cut(s) 629
AciI CCGC 8 cut(s) 11, 57, 135, 138, 300, 332, 1413, 1590
AclWI GGATC 2 cut(s) 369, 1295
AcoI YGGCCR 2 cut(s) 463, 1417
AcsI RAATTY 1 cut(s) 1385
AcuI CTGAAG 2 cut(s) 377, 585
AcyI GRCGYC 4 cut(s) 8, 103, 171, 222
AfaI GTAC 3 cut(s) 1068, 1316, 1397
AfiI CCNNNNNNNGG 6 cut(s) 241, 284, 374, 434, 503, 1675
AgeI ACCGGT 1 cut(s) 427
AgsI TTSAA 5 cut(s) 407, 527, 692, 796, 1345
AjnI CCWGG 4 cut(s) 277, 451, 762, 1366
Alw21I GWGCWC 4 cut(s) 894, 1406, 1473, 1707
Alw26I GTCTC 2 cut(s) 639, 996
Alw44I GTGCAC 1 cut(s) 1402
AlwI GGATC 2 cut(s) 369, 1295
AlwNI CAGNNNCTG 2 cut(s) 1562, 1737
AoxI GGCC 3 cut(s) 463, 1417, 1426
ApaLI GTGCAC 1 cut(s) 1402
ApeKI GCWGC 4 cut(s) 296, 1202, 1646, 1655
ApoI RAATTY 1 cut(s) 1385
AseI ATTAAT 1 cut(s) 539
AsiGI ACCGGT 1 cut(s) 427
Asp700I GAANNNNTTC 1 cut(s) 249
Asp718I GGTACC 1 cut(s) 1395
AspA2I CCTAGG 1 cut(s) 211
AspLEI GCGC 2 cut(s) 10, 173
AspS9I GGNCC 2 cut(s) 425, 1370
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 5 cut(s) 80, 163, 442, 823, 922
AvaII GGWCC 2 cut(s) 425, 1370
AvrII CCTAGG 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 1406
BanI GGYRCC 4 cut(s) 7, 170, 1395, 1681
BanII GRGCYC 1 cut(s) 1473
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 4 cut(s) 894, 1406, 1473, 1707
BbvI GCAGC 4 cut(s) 283, 1189, 1633, 1642
BccI CCATC 6 cut(s) 322, 501, 570, 953, 1505, 1677
BceAI ACGGC 2 cut(s) 184, 450
BciT130I CCWGG 4 cut(s) 279, 453, 764, 1368
BciVI GTATCC 1 cut(s) 759
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 639, 996
BfaI CTAG 6 cut(s) 212, 269, 1128, 1263, 1584, 1652
BfmI CTRYAG 2 cut(s) 930, 1203
BfoI RGCGCY 2 cut(s) 11, 174
BfuI GTATCC 1 cut(s) 759
BglI GCCNNNNNGGC 2 cut(s) 213, 1425
BglII AGATCT 1 cut(s) 850
BisI GCNGC 7 cut(s) 11, 138, 297, 300, 1203, 1647, 1656
BlnI CCTAGG 1 cut(s) 211
BlsI GCNGC 7 cut(s) 12, 139, 298, 301, 1204, 1648, 1657
Bme1390I CCNGG 5 cut(s) 148, 279, 453, 764, 1368
Bme18I GGWCC 2 cut(s) 425, 1370
BmgT120I GGNCC 2 cut(s) 425, 1370
BmiI GGNNCC 4 cut(s) 9, 172, 1397, 1683
BmrFI CCNGG 5 cut(s) 148, 279, 453, 764, 1368
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 6 cut(s) 668, 1096, 1098, 1180, 1549, 1649
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 3 cut(s) 474, 1245, 1500
BpuMI CCSGG 1 cut(s) 148
BsaBI GATNNNNATC 1 cut(s) 1048
BsaHI GRCGYC 4 cut(s) 8, 103, 171, 222
BsaJI CCNNGG 4 cut(s) 147, 211, 303, 1366
BsaWI WCCGGW 2 cut(s) 241, 427
BsaXI ACNNNNNCTCC 6 cut(s) 381, 411, 840, 870, 1694, 1724
Bsc4I CCNNNNNNNGG 6 cut(s) 241, 284, 374, 434, 503, 1675
Bse118I RCCGGY 2 cut(s) 173, 427
Bse1I ACTGG 4 cut(s) 623, 997, 1228, 1399
Bse3DI GCAATG 1 cut(s) 184
Bse8I GATNNNNATC 1 cut(s) 1048
BseBI CCWGG 4 cut(s) 279, 453, 764, 1368
BseDI CCNNGG 4 cut(s) 147, 211, 303, 1366
BseGI GGATG 5 cut(s) 50, 313, 333, 512, 553
BseJI GATNNNNATC 1 cut(s) 1048
BseLI CCNNNNNNNGG 6 cut(s) 241, 284, 374, 434, 503, 1675
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 1569, 1629
BseNI ACTGG 4 cut(s) 623, 997, 1228, 1399
BseRI GAGGAG 2 cut(s) 121, 1481
BseSI GKGCMC 1 cut(s) 1406
BseXI GCAGC 4 cut(s) 283, 1189, 1633, 1642
BshFI GGCC 3 cut(s) 465, 1419, 1428
BshNI GGYRCC 4 cut(s) 7, 170, 1395, 1681
BshTI ACCGGT 1 cut(s) 427
BsiHKAI GWGCWC 4 cut(s) 894, 1406, 1473, 1707
BsiSI CCGG 4 cut(s) 147, 174, 242, 428
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 6 cut(s) 241, 284, 374, 434, 503, 1675
BsmAI GTCTC 2 cut(s) 639, 996
BsmFI GGGAC 1 cut(s) 90
BsmI GAATGC 4 cut(s) 202, 262, 517, 1569
BsnI GGCC 3 cut(s) 465, 1419, 1428
Bsp1286I GDGCHC 4 cut(s) 894, 1406, 1473, 1707
Bsp1407I TGTACA 1 cut(s) 1314
Bsp143I GATC 4 cut(s) 361, 850, 1300, 1551
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 8 cut(s) 11, 57, 135, 138, 300, 332, 1413, 1590
BspANI GGCC 3 cut(s) 465, 1419, 1428
BspCNI CTCAG 2 cut(s) 1568, 1628
BspLI GGNNCC 4 cut(s) 9, 172, 1397, 1683
BspMAI CTGCAG 1 cut(s) 1207
BspPI GGATC 2 cut(s) 369, 1295
BspT107I GGYRCC 4 cut(s) 7, 170, 1395, 1681
BsrDI GCAATG 1 cut(s) 184
BsrFI RCCGGY 2 cut(s) 173, 427
BsrGI TGTACA 1 cut(s) 1314
BsrI ACTGG 4 cut(s) 623, 997, 1228, 1399
BssAI RCCGGY 2 cut(s) 173, 427
BssECI CCNNGG 4 cut(s) 147, 211, 303, 1366
BssMI GATC 4 cut(s) 361, 850, 1300, 1551
BssNI GRCGYC 4 cut(s) 8, 103, 171, 222
BssT1I CCWWGG 2 cut(s) 211, 303
Bst2UI CCWGG 4 cut(s) 279, 453, 764, 1368
Bst4CI ACNGT 4 cut(s) 42, 948, 1078, 1408
Bst6I CTCTTC 2 cut(s) 28, 411
BstACI GRCGYC 4 cut(s) 8, 103, 171, 222
BstAPI GCANNNNNTGC 2 cut(s) 1199, 1646
BstAUI TGTACA 1 cut(s) 1314
BstC8I GCNNGC 5 cut(s) 175, 684, 939, 1118, 1452
BstDEI CTNAG 4 cut(s) 804, 1249, 1555, 1615
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 5 cut(s) 50, 313, 333, 512, 553
BstH2I RGCGCY 2 cut(s) 11, 174
BstHHI GCGC 2 cut(s) 10, 173
BstKTI GATC 4 cut(s) 364, 853, 1303, 1554
BstMAI GTCTC 2 cut(s) 639, 996
BstMBI GATC 4 cut(s) 361, 850, 1300, 1551
BstNI CCWGG 4 cut(s) 279, 453, 764, 1368
BstNSI RCATGY 1 cut(s) 686
BstSCI CCNGG 5 cut(s) 146, 277, 451, 762, 1366
BstSFI CTRYAG 2 cut(s) 930, 1203
BstSLI GKGCMC 1 cut(s) 1406
BstV1I GCAGC 4 cut(s) 283, 1189, 1633, 1642
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 3 cut(s) 465, 1419, 1428
BtgI CCRYGG 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 1203
BtsCI GGATG 5 cut(s) 50, 313, 333, 512, 553
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 3 cut(s) 193, 1406, 1701
Cac8I GCNNGC 5 cut(s) 175, 684, 939, 1118, 1452
CaiI CAGNNNCTG 2 cut(s) 1562, 1737
CfoI GCGC 2 cut(s) 10, 173
Cfr10I RCCGGY 2 cut(s) 173, 427
Cfr13I GGNCC 2 cut(s) 425, 1370
Csp6I GTAC 3 cut(s) 1067, 1315, 1396
CspAI ACCGGT 1 cut(s) 427
CviAII CATG 6 cut(s) 304, 566, 595, 683, 1027, 1392
CviQI GTAC 3 cut(s) 1067, 1315, 1396
DdeI CTNAG 4 cut(s) 804, 1249, 1555, 1615
DinI GGCGCC 2 cut(s) 9, 172
DpnI GATC 4 cut(s) 363, 852, 1302, 1553
DpnII GATC 4 cut(s) 361, 850, 1300, 1551
EaeI YGGCCR 2 cut(s) 463, 1417
Eam1104I CTCTTC 2 cut(s) 28, 411
EarI CTCTTC 2 cut(s) 28, 411
EciI GGCGGA 2 cut(s) 124, 347
Ecl136II GAGCTC 1 cut(s) 1471
Eco130I CCWWGG 2 cut(s) 211, 303
Eco24I GRGCYC 1 cut(s) 1473
Eco32I GATATC 1 cut(s) 1260
Eco47I GGWCC 2 cut(s) 425, 1370
Eco53kI GAGCTC 1 cut(s) 1471
Eco57I CTGAAG 2 cut(s) 377, 585
EcoICRI GAGCTC 1 cut(s) 1471
EcoRII CCWGG 4 cut(s) 277, 451, 762, 1366
EcoRV GATATC 1 cut(s) 1260
EcoT14I CCWWGG 2 cut(s) 211, 303
EcoT22I ATGCAT 1 cut(s) 1642
EcoT38I GRGCYC 1 cut(s) 1473
EgeI GGCGCC 2 cut(s) 9, 172
EheI GGCGCC 2 cut(s) 9, 172
ErhI CCWWGG 2 cut(s) 211, 303
FaeI CATG 6 cut(s) 307, 569, 598, 686, 1030, 1395
FalI AAGNNNNNCTT 4 cut(s) 775, 807, 1691, 1723
FaqI GGGAC 1 cut(s) 90
FatI CATG 6 cut(s) 303, 565, 594, 682, 1026, 1391
FblI GTMKAC 1 cut(s) 629
Fnu4HI GCNGC 7 cut(s) 11, 138, 297, 300, 1203, 1647, 1656
FokI GGATG 5 cut(s) 37, 320, 340, 519, 560
FriOI GRGCYC 1 cut(s) 1473
Fsp4HI GCNGC 7 cut(s) 11, 138, 297, 300, 1203, 1647, 1656
FspBI CTAG 6 cut(s) 212, 269, 1128, 1263, 1584, 1652
GlaI GCGC 2 cut(s) 9, 172
GluI GCNGC 7 cut(s) 11, 138, 297, 300, 1203, 1647, 1656
GsuI CTGGAG 3 cut(s) 474, 1245, 1500
HaeII RGCGCY 2 cut(s) 11, 174
HaeIII GGCC 3 cut(s) 465, 1419, 1428
HapII CCGG 4 cut(s) 147, 174, 242, 428
HhaI GCGC 2 cut(s) 10, 173
Hin1I GRCGYC 4 cut(s) 8, 103, 171, 222
Hin1II CATG 6 cut(s) 307, 569, 598, 686, 1030, 1395
Hin6I GCGC 2 cut(s) 8, 171
HinP1I GCGC 2 cut(s) 8, 171
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 7 cut(s) 143, 154, 407, 473, 533, 1324, 1734
HpaII CCGG 4 cut(s) 147, 174, 242, 428
HphI GGTGA 5 cut(s) 80, 163, 442, 823, 922
Hpy166II GTNNAC 3 cut(s) 630, 1315, 1404
Hpy188III TCNNGA 5 cut(s) 824, 1001, 1263, 1345, 1627
Hpy8I GTNNAC 3 cut(s) 630, 1315, 1404
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 9 cut(s) 77, 352, 609, 663, 781, 1418, 1516, 1669, 1693
HpyCH4III ACNGT 4 cut(s) 42, 948, 1078, 1408
HpyCH4IV ACGT 2 cut(s) 103, 222
HpyF3I CTNAG 4 cut(s) 804, 1249, 1555, 1615
HpySE526I ACGT 2 cut(s) 103, 222
Hsp92I GRCGYC 4 cut(s) 8, 103, 171, 222
Hsp92II CATG 6 cut(s) 307, 569, 598, 686, 1030, 1395
HspAI GCGC 2 cut(s) 8, 171
KasI GGCGCC 2 cut(s) 7, 170
KpnI GGTACC 1 cut(s) 1399
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 4 cut(s) 361, 850, 1300, 1551
LmnI GCTCC 4 cut(s) 402, 1468, 1475, 1702
Lsp1109I GCAGC 4 cut(s) 283, 1189, 1633, 1642
LweI GCATC 6 cut(s) 668, 1096, 1098, 1180, 1549, 1649
MaeI CTAG 6 cut(s) 212, 269, 1128, 1263, 1584, 1652
MaeII ACGT 2 cut(s) 103, 222
MaeIII GTNAC 3 cut(s) 151, 859, 1029
MalI GATC 4 cut(s) 363, 852, 1302, 1553
MboI GATC 4 cut(s) 361, 850, 1300, 1551
MboII GAAGA 7 cut(s) 15, 68, 257, 428, 750, 1220, 1394
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 4 cut(s) 894, 1406, 1473, 1707
MluCI AATT 6 cut(s) 94, 540, 649, 692, 1385, 1434
Mly113I GGCGCC 2 cut(s) 8, 171
MlyI GAGTC 2 cut(s) 148, 527
MmeI TCCRAC 2 cut(s) 535, 1060
Mph1103I ATGCAT 1 cut(s) 1642
MroNI GCCGGC 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 6 cut(s) 539, 1073, 1217, 1320, 1541, 1712
MslI CAYNNNNRTG 2 cut(s) 308, 1574
MspI CCGG 4 cut(s) 147, 174, 242, 428
MspR9I CCNGG 5 cut(s) 148, 279, 453, 764, 1368
MunI CAATTG 1 cut(s) 1434
Mva1269I GAATGC 4 cut(s) 202, 262, 517, 1569
MvaI CCWGG 4 cut(s) 279, 453, 764, 1368
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 2 cut(s) 8, 171
NciI CCSGG 1 cut(s) 148
NcoI CCATGG 1 cut(s) 303
NdeII GATC 4 cut(s) 361, 850, 1300, 1551
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 6 cut(s) 307, 569, 598, 686, 1030, 1395
NlaIV GGNNCC 4 cut(s) 9, 172, 1397, 1683
NmeAIII GCCGAG 1 cut(s) 1445
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 1642
NspI RCATGY 1 cut(s) 686
PaeI GCATGC 1 cut(s) 686
PctI GAATGC 4 cut(s) 202, 262, 517, 1569
PdiI GCCGGC 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 5 cut(s) 143, 407, 473, 1324, 1734
PflMI CCANNNNNTGG 2 cut(s) 374, 503
PinAI ACCGGT 1 cut(s) 427
PkrI GCNGC 7 cut(s) 12, 139, 298, 301, 1204, 1648, 1657
PleI GAGTC 2 cut(s) 148, 527
PluTI GGCGCC 2 cut(s) 11, 174
PpsI GAGTC 2 cut(s) 148, 527
PshBI ATTAAT 1 cut(s) 539
Psp124BI GAGCTC 1 cut(s) 1473
Psp6I CCWGG 4 cut(s) 277, 451, 762, 1366
PspGI CCWGG 4 cut(s) 277, 451, 762, 1366
PspN4I GGNNCC 4 cut(s) 9, 172, 1397, 1683
PspPI GGNCC 2 cut(s) 425, 1370
PstI CTGCAG 1 cut(s) 1207
PstNI CAGNNNCTG 2 cut(s) 1562, 1737
PsuI RGATCY 1 cut(s) 850
RsaI GTAC 3 cut(s) 1068, 1316, 1397
RsaNI GTAC 3 cut(s) 1067, 1315, 1396
RseI CAYNNNNRTG 2 cut(s) 308, 1574
SacI GAGCTC 1 cut(s) 1473
SaqAI TTAA 6 cut(s) 539, 1073, 1217, 1320, 1541, 1712
SatI GCNGC 7 cut(s) 11, 138, 297, 300, 1203, 1647, 1656
Sau3AI GATC 4 cut(s) 361, 850, 1300, 1551
Sau96I GGNCC 2 cut(s) 425, 1370
SchI GAGTC 2 cut(s) 148, 527
ScrFI CCNGG 5 cut(s) 148, 279, 453, 764, 1368
SduI GDGCHC 4 cut(s) 894, 1406, 1473, 1707
SfaNI GCATC 6 cut(s) 668, 1096, 1098, 1180, 1549, 1649
SfcI CTRYAG 2 cut(s) 930, 1203
SfiI GGCCNNNNNGGCC 1 cut(s) 1425
SfoI GGCGCC 2 cut(s) 9, 172
SinI GGWCC 2 cut(s) 425, 1370
SmiMI CAYNNNNRTG 2 cut(s) 308, 1574
SphI GCATGC 1 cut(s) 686
Sse9I AATT 6 cut(s) 94, 540, 649, 692, 1385, 1434
SsiI CCGC 8 cut(s) 11, 57, 135, 138, 300, 332, 1413, 1590
SspDI GGCGCC 2 cut(s) 7, 170
SspI AATATT 3 cut(s) 988, 1151, 1214
SspMI CTAG 6 cut(s) 212, 269, 1128, 1263, 1584, 1652
SstI GAGCTC 1 cut(s) 1473
StyD4I CCNGG 5 cut(s) 146, 277, 451, 762, 1366
StyI CCWWGG 2 cut(s) 211, 303
TaaI ACNGT 4 cut(s) 42, 948, 1078, 1408
TaiI ACGT 2 cut(s) 106, 225
TaqI TCGA 5 cut(s) 162, 219, 364, 943, 1000
TasI AATT 6 cut(s) 94, 540, 649, 692, 1385, 1434
TatI WGTACW 1 cut(s) 1314
TauI GCSGC 3 cut(s) 13, 140, 302
TfiI GAWTC 5 cut(s) 143, 407, 473, 1324, 1734
Tru1I TTAA 6 cut(s) 539, 1073, 1217, 1320, 1541, 1712
Tru9I TTAA 6 cut(s) 539, 1073, 1217, 1320, 1541, 1712
TscAI CASTG 3 cut(s) 193, 1406, 1701
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 4 cut(s) 296, 1202, 1646, 1655
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 4 cut(s) 336, 465, 569, 621
TspGWI ACGGA 2 cut(s) 109, 113
TspRI CASTG 3 cut(s) 193, 1406, 1701
Van91I CCANNNNNTGG 2 cut(s) 374, 503
VneI GTGCAC 1 cut(s) 1402
VpaK11BI GGWCC 2 cut(s) 425, 1370
VspI ATTAAT 1 cut(s) 539
XapI RAATTY 1 cut(s) 1385
XbaI TCTAGA 1 cut(s) 1262
XceI RCATGY 1 cut(s) 686
XcmI CCANNNNNNNNNTGG 1 cut(s) 504
XmaJI CCTAGG 1 cut(s) 211
XmiI GTMKAC 1 cut(s) 629
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 6 cut(s) 212, 269, 1128, 1263, 1584, 1652
ZraI GACGTC 2 cut(s) 104, 223
Zsp2I ATGCAT 1 cut(s) 1642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.