Rh5CG309600

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003239
Physical Location & Seq
Reverse (-)
34184374 .. 34190979
6606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG309600.1

Sequence Viewer

Length: 1563 bp
ATGGAGGCGCCGCCAATTGTTTCCCCTACCAAAACGCTGCCGTTCCGATCAGCTTCCCCTTCCTCCTCTCTTCTCTTTCGCCGCAAGCATCGTCTCAATTGCCGCCGCAGACCTTCTAGAAGCTTCACCGTCCGTGCCTCCTCCGCTGCTAATGATGATTCAGTGGTGACTCTGCTTGACTACGGTGCCGGCAATGTTCGGAGTGTCAGGAACGCCATTCGCCGCCTGGGCTTCCAAGTCAAAGATGTCCAAACTCCCAAGGATATTTTGAATGCCAACCGCCTAATCTTTCCTGGTGTGGGGGCTTTCGCTGCGGCCATGGATGTGCTGAGCAAGAATGGGATGGCTGAAGCGCTCTGTGAATATATTGAGAAAGATCGGCCGTTTCTAGGCATTTGTCTTGGACTTCAGCTCCTTTTTGAATCCAGTGAGGAAAAGGGACAAGTGAGTGGTCTTGGCTTGATACCTGGAGTGGTTGGGCGTTTTGACTCATCAAATGGTGTTCGAGTGCCACATATCGGGTGGAATGCTTTGCAGATTAGAGAAGATTCTTTAATTTTGGATGATGTTGGAACCCATCACGTCTATTTTGTCCACTCTTACAGAGCAATGCCATCGGATGATAACAAAGAATGGGTTTCATCTACCTGCAACTATGGTGACAATTTCATTGCATCTGTTAGAAGGGGAAATGTGCATGCAGTTCAATTCCACCCTGAGAAAAGTGGAGATGTTGGTCTTTCCATATTGAGAAGATTTCTCTATCCAAACTCACCCTTGGAGAAGAAGCCCACTGAAGGGAAGGGTTCCAAACTTGCAAAGAGGGTTATTGCTTGTCTGGATGTGAGGACGAATGACAAAGGAGATCTTGTTGTAACCAAAGGAGACCAATATGACGTAAGAGAGCATACAAAAGAGAATGAGGTGAGAGAACTTGGAAAGCCTGTGGAGCTTGCTGGACAGTATTACACAGATGGAGCTGATGAGGTCAGCTTTCTGAATATTACCGGTTTCCGGGACTTCCCTTTGGGTGACTTGCCTATGCTACAGGTATTGAGATATGCATCAGAAAATGTTTTCGTGCCATTAACAGTTGGAGGTGGCATTAGAGATTTTACTGATGCAACTGGCAGGCACTATTCTAGTTTGGAAGTTGCTTCAGAATATTTTAGATGTGGGGCTGATAAGATCTCCATTGGAAGTGATGCAGTTTATGCTGCAGAAGAATATTTAAGAACTGGAGTGAAAAGTGGAAAAAGCAGCTTAGAGCAGATATCCAGAGTTTACGGAAATCAGGCTGTGGTTGTAAGCATTGATCCTTGCAGAGTGTATGTTAAAAATCAAAATGATGTAAAGTTCAAGACTATAAGAGTAACAAATCCAGGTCCAAATGGCGAAGAATATGCTTGGTATCAGTGTACAGTTAGCGGTGGGCGGGAAGGCAGACCAATTGGAGCGTATGAGCTTGCAAAAGCAGTTGAGGAGCTAGGAGCTGGAGAAATTCTGCTAAACTGCATTGATTGCGATGGTAAAGTCACTTTTTGCACTCCTTATGCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

520

Amino Acids

57.2

Weight (kDa)

7.51

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 58 - 254 1.1e-23 Glutamine amidotransferase class-I
SNO PF01174 64 - 244 7e-08 SNO glutamine amidotransferase family
GATase_3 PF07685 79 - 157 2.4e-08 CobB/CobQ-like glutamine amidotransferase domain
His_biosynth PF00977 275 - 511 2.3e-23 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 656
AccB1I GGYRCC 2 cut(s) 7, 185
AclWI GGATC 1 cut(s) 1310
AcoI YGGCCR 2 cut(s) 315, 380
AcsI RAATTY 1 cut(s) 1500
AcuI CTGAAG 4 cut(s) 369, 392, 816, 1143
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 1 cut(s) 1420
AfeI AGCGCT 1 cut(s) 354
AfiI CCNNNNNNNGG 6 cut(s) 299, 389, 518, 797, 798, 1014
AgeI ACCGGT 1 cut(s) 1007
AgsI TTSAA 4 cut(s) 271, 422, 707, 1360
AjiI CACGTC 1 cut(s) 583
AjnI CCWGG 4 cut(s) 225, 292, 466, 1381
Alw26I GTCTC 2 cut(s) 98, 879
AlwI GGATC 1 cut(s) 1310
Aor51HI AGCGCT 1 cut(s) 354
AoxI GGCC 2 cut(s) 315, 380
ApeKI GCWGC 5 cut(s) 37, 146, 311, 1217, 1260
ApoI RAATTY 1 cut(s) 1500
AsiGI ACCGGT 1 cut(s) 1007
AspLEI GCGC 2 cut(s) 10, 355
AspS9I GGNCC 1 cut(s) 1385
AsuC2I CCSGG 1 cut(s) 1016
AsuHPI GGTGA 6 cut(s) 118, 178, 671, 765, 937, 1043
AvaII GGWCC 1 cut(s) 1385
BanI GGYRCC 2 cut(s) 7, 185
BbvI GCAGC 5 cut(s) 24, 133, 298, 1204, 1272
BccI CCATC 5 cut(s) 337, 585, 622, 968, 1520
BceAI ACGGC 2 cut(s) 25, 367
BcgI CGANNNNNNTGC 4 cut(s) 597, 631, 1385, 1419
BciT130I CCWGG 4 cut(s) 227, 294, 468, 1383
BcnI CCSGG 1 cut(s) 1016
BcoDI GTCTC 2 cut(s) 98, 879
BfaI CTAG 4 cut(s) 117, 389, 1143, 1487
BfmI CTRYAG 2 cut(s) 1046, 1218
BfoI RGCGCY 2 cut(s) 11, 356
BfuAI ACCTGC 1 cut(s) 656
BglI GCCNNNNNGGC 1 cut(s) 228
BglII AGATCT 2 cut(s) 865, 1188
BlpI GCTNAGC 1 cut(s) 329
Bme1390I CCNGG 5 cut(s) 227, 294, 468, 1016, 1383
Bme18I GGWCC 1 cut(s) 1385
BmgBI CACGTC 1 cut(s) 583
BmgT120I GGNCC 1 cut(s) 1385
BmiI GGNNCC 4 cut(s) 9, 187, 574, 808
BmrFI CCNGG 5 cut(s) 227, 294, 468, 1016, 1383
BmsI GCATC 5 cut(s) 97, 683, 1073, 1111, 1195
BpmI CTGGAG 3 cut(s) 489, 1260, 1515
Bpu1102I GCTNAGC 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 1016
BsaBI GATNNNNATC 1 cut(s) 1063
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 879
BsaJI CCNNGG 4 cut(s) 226, 258, 318, 777
BsaWI WCCGGW 1 cut(s) 1007
BsaXI ACNNNNNCTCC 4 cut(s) 396, 426, 855, 885
Bsc4I CCNNNNNNNGG 6 cut(s) 299, 389, 518, 797, 798, 1014
Bse118I RCCGGY 2 cut(s) 188, 1007
Bse1I ACTGG 3 cut(s) 426, 1132, 1243
Bse3DI GCAATG 3 cut(s) 199, 615, 669
Bse8I GATNNNNATC 1 cut(s) 1063
BseBI CCWGG 4 cut(s) 227, 294, 468, 1383
BseDI CCNNGG 4 cut(s) 226, 258, 318, 777
BseGI GGATG 5 cut(s) 328, 348, 568, 625, 847
BseJI GATNNNNATC 1 cut(s) 1063
BseLI CCNNNNNNNGG 6 cut(s) 299, 389, 518, 797, 798, 1014
BseMI GCAATG 3 cut(s) 199, 615, 669
BseMII CTCAG 2 cut(s) 320, 708
BseNI ACTGG 3 cut(s) 426, 1132, 1243
BseRI GAGGAG 3 cut(s) 55, 130, 1496
BseX3I CGGCCG 1 cut(s) 380
BseXI GCAGC 5 cut(s) 24, 133, 298, 1204, 1272
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 2 cut(s) 317, 382
BshNI GGYRCC 2 cut(s) 7, 185
BshTI ACCGGT 1 cut(s) 1007
BsiEI CGRYCG 1 cut(s) 383
BsiSI CCGG 3 cut(s) 189, 1008, 1015
BslFI GGGAC 2 cut(s) 453, 1031
BslI CCNNNNNNNGG 6 cut(s) 299, 389, 518, 797, 798, 1014
BsmAI GTCTC 2 cut(s) 98, 879
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 2 cut(s) 453, 1031
BsmI GAATGC 2 cut(s) 277, 532
BsnI GGCC 2 cut(s) 317, 382
Bso31I GGTCTC 1 cut(s) 879
Bsp1407I TGTACA 1 cut(s) 1418
Bsp143I GATC 5 cut(s) 47, 376, 865, 1188, 1315
Bsp1720I GCTNAGC 1 cut(s) 329
Bsp19I CCATGG 1 cut(s) 318
BspANI GGCC 2 cut(s) 317, 382
BspCNI CTCAG 2 cut(s) 321, 709
BspLI GGNNCC 4 cut(s) 9, 187, 574, 808
BspMAI CTGCAG 1 cut(s) 1222
BspMI ACCTGC 1 cut(s) 656
BspPI GGATC 1 cut(s) 1310
BspT107I GGYRCC 2 cut(s) 7, 185
BspTNI GGTCTC 1 cut(s) 879
BsrDI GCAATG 3 cut(s) 199, 615, 669
BsrFI RCCGGY 2 cut(s) 188, 1007
BsrGI TGTACA 1 cut(s) 1418
BsrI ACTGG 3 cut(s) 426, 1132, 1243
BssAI RCCGGY 2 cut(s) 188, 1007
BssECI CCNNGG 4 cut(s) 226, 258, 318, 777
BssMI GATC 5 cut(s) 47, 376, 865, 1188, 1315
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 3 cut(s) 258, 318, 777
Bst2UI CCWGG 4 cut(s) 227, 294, 468, 1383
Bst4CI ACNGT 5 cut(s) 130, 185, 963, 1093, 1423
Bst6I CTCTTC 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 2 cut(s) 1214, 1521
BstAUI TGTACA 1 cut(s) 1418
BstC8I GCNNGC 7 cut(s) 86, 190, 699, 954, 1133, 1467, 1558
BstDEI CTNAG 3 cut(s) 329, 717, 1264
BstDSI CCRYGG 1 cut(s) 318
BstF5I GGATG 5 cut(s) 328, 348, 568, 625, 847
BstH2I RGCGCY 2 cut(s) 11, 356
BstHHI GCGC 2 cut(s) 10, 355
BstKTI GATC 5 cut(s) 50, 379, 868, 1191, 1318
BstMAI GTCTC 2 cut(s) 98, 879
BstMBI GATC 5 cut(s) 47, 376, 865, 1188, 1315
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 6 cut(s) 143, 228, 311, 949, 1214, 1521
BstNI CCWGG 4 cut(s) 227, 294, 468, 1383
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 5 cut(s) 225, 292, 466, 1014, 1381
BstSFI CTRYAG 2 cut(s) 1046, 1218
BstV1I GCAGC 5 cut(s) 24, 133, 298, 1204, 1272
BstX2I RGATCY 2 cut(s) 865, 1188
BstYI RGATCY 2 cut(s) 865, 1188
BstZI CGGCCG 1 cut(s) 380
BsuRI GGCC 2 cut(s) 317, 382
BtgI CCRYGG 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 1539
BtrI CACGTC 1 cut(s) 583
BtsCI GGATG 5 cut(s) 328, 348, 568, 625, 847
BtsIMutI CAGTG 4 cut(s) 168, 433, 792, 1421
BveI ACCTGC 1 cut(s) 656
Cac8I GCNNGC 7 cut(s) 86, 190, 699, 954, 1133, 1467, 1558
CfoI GCGC 2 cut(s) 10, 355
Cfr10I RCCGGY 2 cut(s) 188, 1007
Cfr13I GGNCC 1 cut(s) 1385
Csp6I GTAC 1 cut(s) 1419
CspAI ACCGGT 1 cut(s) 1007
CviAII CATG 2 cut(s) 319, 698
CviQI GTAC 1 cut(s) 1419
DdeI CTNAG 3 cut(s) 329, 717, 1264
DinI GGCGCC 1 cut(s) 9
DpnI GATC 5 cut(s) 49, 378, 867, 1190, 1317
DpnII GATC 5 cut(s) 47, 376, 865, 1188, 1315
EaeI YGGCCR 2 cut(s) 315, 380
EagI CGGCCG 1 cut(s) 380
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
EclXI CGGCCG 1 cut(s) 380
Eco130I CCWWGG 3 cut(s) 258, 318, 777
Eco31I GGTCTC 1 cut(s) 879
Eco32I GATATC 1 cut(s) 1275
Eco47I GGWCC 1 cut(s) 1385
Eco47III AGCGCT 1 cut(s) 354
Eco52I CGGCCG 1 cut(s) 380
Eco57I CTGAAG 4 cut(s) 369, 392, 816, 1143
EcoRII CCWGG 4 cut(s) 225, 292, 466, 1381
EcoRV GATATC 1 cut(s) 1275
EcoT14I CCWWGG 3 cut(s) 258, 318, 777
EcoT22I ATGCAT 1 cut(s) 1066
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 3 cut(s) 258, 318, 777
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 2 cut(s) 322, 701
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FaqI GGGAC 2 cut(s) 453, 1031
FatI CATG 2 cut(s) 318, 697
FauI CCCGC 1 cut(s) 1428
FokI GGATG 5 cut(s) 335, 355, 575, 632, 854
FspBI CTAG 4 cut(s) 117, 389, 1143, 1487
GlaI GCGC 2 cut(s) 9, 354
GsuI CTGGAG 3 cut(s) 489, 1260, 1515
HaeII RGCGCY 2 cut(s) 11, 356
HaeIII GGCC 2 cut(s) 317, 382
HapII CCGG 3 cut(s) 189, 1008, 1015
HhaI GCGC 2 cut(s) 10, 355
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 2 cut(s) 322, 701
Hin6I GCGC 2 cut(s) 8, 353
HinP1I GCGC 2 cut(s) 8, 353
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 5 cut(s) 158, 169, 422, 488, 548
HpaII CCGG 3 cut(s) 189, 1008, 1015
HphI GGTGA 6 cut(s) 118, 178, 671, 765, 937, 1043
Hpy166II GTNNAC 3 cut(s) 595, 1285, 1419
Hpy188I TCNGA 6 cut(s) 47, 201, 619, 999, 1069, 1162
Hpy188III TCNNGA 5 cut(s) 117, 208, 839, 1278, 1360
Hpy8I GTNNAC 3 cut(s) 595, 1285, 1419
HpyAV CCTTC 6 cut(s) 69, 123, 678, 791, 796, 1433
HpyCH4III ACNGT 5 cut(s) 130, 185, 963, 1093, 1423
HpyCH4IV ACGT 2 cut(s) 582, 897
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 228, 311, 949, 1214, 1521
HpyF3I CTNAG 3 cut(s) 329, 717, 1264
HpySE526I ACGT 2 cut(s) 582, 897
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 2 cut(s) 322, 701
HspAI GCGC 2 cut(s) 8, 353
KasI GGCGCC 1 cut(s) 7
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Kzo9I GATC 5 cut(s) 47, 376, 865, 1188, 1315
LmnI GCTCC 6 cut(s) 417, 949, 977, 1454, 1483, 1490
Lsp1109I GCAGC 5 cut(s) 24, 133, 298, 1204, 1272
LweI GCATC 5 cut(s) 97, 683, 1073, 1111, 1195
MaeI CTAG 4 cut(s) 117, 389, 1143, 1487
MaeII ACGT 2 cut(s) 582, 897
MaeIII GTNAC 6 cut(s) 166, 659, 874, 1031, 1372, 1534
MalI GATC 5 cut(s) 49, 378, 867, 1190, 1317
MboI GATC 5 cut(s) 47, 376, 865, 1188, 1315
MboII GAAGA 6 cut(s) 62, 557, 765, 796, 1235, 1409
MfeI CAATTG 3 cut(s) 15, 97, 1449
MflI RGATCY 2 cut(s) 865, 1188
MluCI AATT 7 cut(s) 15, 97, 555, 664, 707, 1449, 1500
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 2 cut(s) 163, 482
MmeI TCCRAC 2 cut(s) 550, 1075
Mph1103I ATGCAT 1 cut(s) 1066
MroNI GCCGGC 1 cut(s) 188
MseI TTAA 4 cut(s) 554, 1088, 1232, 1335
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 3 cut(s) 189, 1008, 1015
MspR9I CCNGG 5 cut(s) 227, 294, 468, 1016, 1383
MunI CAATTG 3 cut(s) 15, 97, 1449
Mva1269I GAATGC 2 cut(s) 277, 532
MvaI CCWGG 4 cut(s) 227, 294, 468, 1383
MwoI GCNNNNNNNGC 6 cut(s) 143, 228, 311, 949, 1214, 1521
NaeI GCCGGC 1 cut(s) 190
NarI GGCGCC 1 cut(s) 8
NciI CCSGG 1 cut(s) 1016
NcoI CCATGG 1 cut(s) 318
NdeII GATC 5 cut(s) 47, 376, 865, 1188, 1315
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 2 cut(s) 322, 701
NlaIV GGNNCC 4 cut(s) 9, 187, 574, 808
NmuCI GTSAC 4 cut(s) 166, 659, 1031, 1534
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 1 cut(s) 701
PaeI GCATGC 1 cut(s) 701
PctI GAATGC 2 cut(s) 277, 532
PdiI GCCGGC 1 cut(s) 190
PfeI GAWTC 3 cut(s) 158, 422, 548
PfoI TCCNGGA 1 cut(s) 1014
PinAI ACCGGT 1 cut(s) 1007
PleI GAGTC 2 cut(s) 163, 482
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 2 cut(s) 163, 482
Psp6I CCWGG 4 cut(s) 225, 292, 466, 1381
PspGI CCWGG 4 cut(s) 225, 292, 466, 1381
PspN4I GGNNCC 4 cut(s) 9, 187, 574, 808
PspPI GGNCC 1 cut(s) 1385
PstI CTGCAG 1 cut(s) 1222
PsuI RGATCY 2 cut(s) 865, 1188
RsaI GTAC 1 cut(s) 1420
RsaNI GTAC 1 cut(s) 1419
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 4 cut(s) 554, 1088, 1232, 1335
Sau3AI GATC 5 cut(s) 47, 376, 865, 1188, 1315
Sau96I GGNCC 1 cut(s) 1385
SchI GAGTC 2 cut(s) 163, 482
ScrFI CCNGG 5 cut(s) 227, 294, 468, 1016, 1383
SfaNI GCATC 5 cut(s) 97, 683, 1073, 1111, 1195
SfcI CTRYAG 2 cut(s) 1046, 1218
SfoI GGCGCC 1 cut(s) 9
SinI GGWCC 1 cut(s) 1385
SmiMI CAYNNNNRTG 1 cut(s) 323
SphI GCATGC 1 cut(s) 701
Sse9I AATT 7 cut(s) 15, 97, 555, 664, 707, 1449, 1500
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 3 cut(s) 1003, 1166, 1229
SspMI CTAG 4 cut(s) 117, 389, 1143, 1487
StyD4I CCNGG 5 cut(s) 225, 292, 466, 1014, 1381
StyI CCWWGG 3 cut(s) 258, 318, 777
TaaI ACNGT 5 cut(s) 130, 185, 963, 1093, 1423
TaiI ACGT 2 cut(s) 585, 900
TaqI TCGA 1 cut(s) 505
TasI AATT 7 cut(s) 15, 97, 555, 664, 707, 1449, 1500
TatI WGTACW 1 cut(s) 1418
TauI GCSGC 6 cut(s) 13, 84, 105, 108, 225, 317
TfiI GAWTC 3 cut(s) 158, 422, 548
Tru1I TTAA 4 cut(s) 554, 1088, 1232, 1335
Tru9I TTAA 4 cut(s) 554, 1088, 1232, 1335
TscAI CASTG 4 cut(s) 168, 433, 799, 1421
TseFI GTSAC 4 cut(s) 166, 659, 1031, 1534
TseI GCWGC 5 cut(s) 37, 146, 311, 1217, 1260
Tsp45I GTSAC 4 cut(s) 166, 659, 1031, 1534
TspDTI ATGAA 2 cut(s) 630, 658
TspGWI ACGGA 2 cut(s) 122, 1302
TspRI CASTG 4 cut(s) 168, 433, 799, 1421
VpaK11BI GGWCC 1 cut(s) 1385
XapI RAATTY 1 cut(s) 1500
XbaI TCTAGA 1 cut(s) 116
XceI RCATGY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 2 cut(s) 223, 519
XspI CTAG 4 cut(s) 117, 389, 1143, 1487
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.