RchiOBHm_Chr4g0412471

Belongs to the HisA HisF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003239
Physical Location & Seq
Forward (+)
36276061 .. 36280035
3975 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38314

Sequence Viewer

Length: 486 bp
ATGCAAGAATCTGTGGGATGCCTCTCTGACAGTTCTCAATTGGTTTTTCAGGTGAAAAGTGGAAAAAGTAGCTTCGAGCAGATATCTAGAGTTTATGGAAATCAGGCTGTGGTTGTAAGCATTGATCCTTGCAAAGTGTACGTTAAAAATCAAAATGATGTTGAGTTCGAGACTATAAGGGTAACAAACCCAGTTAGCGGTGGGTGGGAAAGCCAACGAATCGGAGCTTATAAGCTTACAAAAGCAGTTGAGGAGCTTGGTGCTGGAGAAATTCTGCTAGACTGCATTGATTATGAAAGTAAAGTCAGGGAAAGGGGATTTGATATAGATTTAGTAAAGCTGATCTCTGATGTGGTGACCATTCCCATGCTTGCAAGTAGTGGTGCTGGTGTAGTTGAACACTTCTCAGAGGTTCTCACGAAAGCAAATGCGTCTGCTGCCTTTGCTGCTGGCATTTTTCATTCCAAGGAGGGATTCATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230
Pfam Domains
Protein Families
GATase His_biosynth

Protein Analysis

161

Amino Acids

17.44

Weight (kDa)

4.93

Isoelectric Point (pI)

20.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_biosynth PF00977 24 - 157 1.4e-16 Histidine biosynthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 1 cut(s) 270
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 197
AgsI TTSAA 1 cut(s) 398
AjuI GAANNNNNNNTTGG 1 cut(s) 458
AluBI AGCT 5 cut(s) 72, 227, 235, 256, 340
AluI AGCT 5 cut(s) 72, 227, 235, 256, 340
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 119
ApeKI GCWGC 2 cut(s) 437, 446
ApoI RAATTY 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 64, 367
BbvI GCAGC 2 cut(s) 424, 433
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 2 cut(s) 87, 278
BisI GCNGC 2 cut(s) 438, 447
BlsI GCNGC 2 cut(s) 439, 448
BmrI ACTGGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 465
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 1 cut(s) 23
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 2 cut(s) 424, 433
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 164
Bsp143I GATC 2 cut(s) 124, 342
BspACI CCGC 1 cut(s) 198
BspCNI CTCAG 1 cut(s) 419
BspPI GGATC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 2 cut(s) 124, 342
BssT1I CCWWGG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 372, 451
BstDEI CTNAG 1 cut(s) 406
BstEII GGTNACC 1 cut(s) 355
BstF5I GGATG 1 cut(s) 23
BstKTI GATC 2 cut(s) 127, 345
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 2 cut(s) 124, 342
BstMWI GCNNNNNNNGC 3 cut(s) 437, 443, 446
BstPI GGTNACC 1 cut(s) 355
BstV1I GCAGC 2 cut(s) 424, 433
BtsCI GGATG 1 cut(s) 23
Cac8I GCNNGC 2 cut(s) 372, 451
CseI GACGC 1 cut(s) 420
Csp6I GTAC 1 cut(s) 139
CviAII CATG 1 cut(s) 367
CviJI RGCY 7 cut(s) 72, 107, 213, 227, 235, 256, 340
CviKI_1 RGCY 7 cut(s) 72, 107, 213, 227, 235, 256, 340
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 406
DpnI GATC 2 cut(s) 126, 344
DpnII GATC 2 cut(s) 124, 342
Eco130I CCWWGG 1 cut(s) 465
Eco32I GATATC 1 cut(s) 84
Eco91I GGTNACC 1 cut(s) 355
EcoO65I GGTNACC 1 cut(s) 355
EcoRV GATATC 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 1 cut(s) 370
FaiI YATR 6 cut(s) 96, 176, 231, 294, 326, 368
FatI CATG 1 cut(s) 366
Fnu4HI GCNGC 2 cut(s) 438, 447
FokI GGATG 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 438, 447
FspBI CTAG 2 cut(s) 87, 278
GluI GCNGC 2 cut(s) 438, 447
GsuI CTGGAG 1 cut(s) 285
HgaI GACGC 1 cut(s) 420
Hin1II CATG 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 3 cut(s) 8, 219, 474
HphI GGTGA 2 cut(s) 64, 367
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 28, 224, 349, 409
Hpy188III TCNNGA 3 cut(s) 87, 169, 418
Hpy8I GTNNAC 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 32
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 4 cut(s) 4, 132, 285, 374
HpyF10VI GCNNNNNNNGC 3 cut(s) 437, 443, 446
HpyF3I CTNAG 1 cut(s) 406
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 370
Kzo9I GATC 2 cut(s) 124, 342
LmnI GCTCC 2 cut(s) 224, 253
LpnPI CCDG 7 cut(s) 35, 89, 204, 249, 292, 372, 435
Lsp1109I GCAGC 2 cut(s) 424, 433
LweI GCATC 1 cut(s) 8
MaeI CTAG 2 cut(s) 87, 278
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 2 cut(s) 181, 355
MalI GATC 2 cut(s) 126, 344
MboI GATC 2 cut(s) 124, 342
MfeI CAATTG 1 cut(s) 38
MluCI AATT 2 cut(s) 38, 270
MnlI CCTC 4 cut(s) 32, 244, 403, 463
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 1 cut(s) 365
MunI CAATTG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 437, 443, 446
NdeII GATC 2 cut(s) 124, 342
NlaIII CATG 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 355
PfeI GAWTC 3 cut(s) 8, 219, 474
PkrI GCNGC 2 cut(s) 439, 448
PsiI TTATAA 1 cut(s) 231
PspEI GGTNACC 1 cut(s) 355
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 365
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 2 cut(s) 438, 447
Sau3AI GATC 2 cut(s) 124, 342
SetI ASST 8 cut(s) 54, 74, 144, 229, 237, 258, 342, 414
SfaNI GCATC 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 365
Sse9I AATT 2 cut(s) 38, 270
SsiI CCGC 1 cut(s) 198
SspMI CTAG 2 cut(s) 87, 278
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 32
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 75, 168
TasI AATT 2 cut(s) 38, 270
TfiI GAWTC 3 cut(s) 8, 219, 474
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 2 cut(s) 437, 446
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 3 cut(s) 309, 449, 466
XapI RAATTY 1 cut(s) 270
XbaI TCTAGA 1 cut(s) 86
XspI CTAG 2 cut(s) 87, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.