FvH4_6g01921

Protein prenyltransferase alpha subunit repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001731
Physical Location & Seq
Reverse (-)
1087836 .. 1091722
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01921.t1

Sequence Viewer

Length: 1371 bp
ATGACTGCTCTCTTATGCTTGAAGCATAAAACTTTCTCCGGCGATTTTCCGGCCACAATGATCGGAGAAGCCAACGCCGGCGACCTTCTCAGGCGATTTGAGAAAATTTTGGAGTCCGATTCTGCAATTGATGAGGTTGGATTTGTTCATCCATCCCAGTTTGTCAACTTGAGTGAAGAGGCGCCCTCATCATCTGGTAGTGTTAGTAATGTGGTGTGCTTGAAAGATGGGACTGCGGTGTTTTGGAATAGGGAGCATAAGTTGGGAATCTCGACGGATGTTCTTATTCCATTGTATAAGGAAGCTAAGCGCGCGATGATGGCAGCAATTCAACAATATAAGGGACTTTATAGTGCATTTGATGTGTCCGGGGTTGAGAGTTCTGTATCTTTGGATGACAGTATTGAAGGTGAAGTCATGAAGCAAAGCAGGGCTGTTTTGTTAGTGAGTTCTGATTATGGCACTGCTTGGAATTCCAGGAAGATGGTGTTGTTGAAGAAGCAAGAGTTCTCAATGTTATTGGATGAACTTCTATTCTCAGCACTTGTTCTTTCATATGCACCTAAATCTGAGCATGCGTGGTGCCACAGGCGATGGGTCATCAAGTTAATTGCTGGAAAGTTTTCAACTTTGCAAGAGATTGTCACCAAAGAGTCTGAGCTGGTGGAGAAAATAGCCGAGAGGTCAAAAATGAATTACCGTGCATGGTATCATCGTTGCTGGTTGGTGTCTTACATGACAACAGAGCAGAGATCTAAAAAGTGGGCTGGCTTGCACGTTGCTGACCATAGTTGCTTCCACTATCGCAGACAGCTAATGCTTAGGATTTTAGAGGACAGATGCTGTAAACAAGAAAGTGTATCTTCTAGTTATAATATTACAATAAATCAACTGTGGAAGGAGGAGCTGGATTGGGATGAAATGTTGATAAAGCGCTACATAGGAAGAGAGGCCTTATGGCTTCATCGGCGCTTCCTATCCTTATTTTGGATAAAGCATTTTTTAACTGGTCATACTGATCTGCCTGGGGATTGGAAGCACAAAACCAGCATGAATAATGACTTAGGTATCTTTATGGATCATGAATTACATCTCCTGCAGTCCTGCTCCATCATTCCAGATGATAGTTTTGATGATTACCAAGCACAGGCTACACATTCTGCTACTTACATGTTATGGTTAATTAAGCAATTCCCTGATTCTGGTAGCATTGAATTACGGGAGAAGCTAAAAATCAAAAACCTGAAAGCCCTGCTGAACAACGCATGCCCTGGAAGATCCTCAACTTGGGATTCTATACTGGCCTACGTCGAAACGTCCGACTATTATGCAACAGAGGTAGACCCGCACCACCCTGCAACTACGAGTTGA

Protein Analysis

457

Amino Acids

52.41

Weight (kDa)

6.42

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPTA PF01239 174 - 202 1.4e-06 Protein prenyltransferase alpha subunit repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 873
AccB1I GGYRCC 2 cut(s) 181, 582
AccI GTMKAC 1 cut(s) 1341
AccII CGCG 2 cut(s) 312, 314
AciI CCGC 2 cut(s) 236, 1346
AclWI GGATC 2 cut(s) 1086, 1272
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 2 cut(s) 105, 472
AcyI GRCGYC 1 cut(s) 182
AfeI AGCGCT 1 cut(s) 935
AfiI CCNNNNNNNGG 4 cut(s) 987, 1147, 1202, 1287
AflIII ACRYGT 1 cut(s) 1170
AgsI TTSAA 7 cut(s) 22, 223, 332, 407, 496, 627, 1214
AjnI CCWGG 3 cut(s) 476, 1024, 1270
AluBI AGCT 5 cut(s) 305, 661, 814, 907, 1228
AluI AGCT 5 cut(s) 305, 661, 814, 907, 1228
AlwI GGATC 2 cut(s) 1086, 1272
AlwNI CAGNNNCTG 1 cut(s) 843
Aor51HI AGCGCT 1 cut(s) 935
AoxI GGCC 3 cut(s) 51, 951, 1302
ApeKI GCWGC 1 cut(s) 323
ApoI RAATTY 2 cut(s) 105, 472
Asp700I GAANNNNTTC 1 cut(s) 622
AspLEI GCGC 5 cut(s) 184, 312, 314, 936, 972
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 422, 637
BaeI ACNNNNGTAYC 2 cut(s) 1051, 1084
BanI GGYRCC 2 cut(s) 181, 582
BbvI GCAGC 1 cut(s) 335
BccI CCATC 6 cut(s) 160, 221, 313, 478, 588, 1118
BcgI CGANNNNNNTGC 2 cut(s) 1310, 1344
BciT130I CCWGG 3 cut(s) 478, 1026, 1272
BcnI CCSGG 1 cut(s) 370
BfaI CTAG 1 cut(s) 867
BfmI CTRYAG 1 cut(s) 1097
BfoI RGCGCY 3 cut(s) 185, 937, 973
BglII AGATCT 1 cut(s) 752
BisI GCNGC 1 cut(s) 324
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 325
Bme1390I CCNGG 4 cut(s) 370, 478, 1026, 1272
BmiI GGNNCC 2 cut(s) 183, 584
BmrFI CCNGG 4 cut(s) 370, 478, 1026, 1272
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 1 cut(s) 830
BmuI ACTGGG 1 cut(s) 151
BplI GAGNNNNNCTC 2 cut(s) 170, 202
Bpu10I CCTNAGC 1 cut(s) 821
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 182
BsaJI CCNNGG 3 cut(s) 369, 1025, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 4 cut(s) 987, 1147, 1202, 1287
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 3 cut(s) 157, 1012, 1305
BseBI CCWGG 3 cut(s) 478, 1026, 1272
BseDI CCNNGG 3 cut(s) 369, 1025, 1270
BseGI GGATG 6 cut(s) 148, 152, 283, 400, 529, 922
BseLI CCNNNNNNNGG 4 cut(s) 987, 1147, 1202, 1287
BseMII CTCAG 4 cut(s) 103, 552, 561, 648
BseNI ACTGG 3 cut(s) 157, 1012, 1305
BsePI GCGCGC 1 cut(s) 310
BseRI GAGGAG 1 cut(s) 917
BseXI GCAGC 1 cut(s) 335
Bsh1236I CGCG 2 cut(s) 312, 314
BshFI GGCC 3 cut(s) 53, 953, 1304
BshNI GGYRCC 2 cut(s) 181, 582
BsiSI CCGG 4 cut(s) 39, 50, 78, 369
BslFI GGGAC 2 cut(s) 244, 357
BslI CCNNNNNNNGG 4 cut(s) 987, 1147, 1202, 1287
BsmFI GGGAC 2 cut(s) 244, 357
BsnI GGCC 3 cut(s) 53, 953, 1304
Bsp143I GATC 5 cut(s) 60, 752, 1018, 1078, 1277
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 2 cut(s) 236, 1346
BspANI GGCC 3 cut(s) 53, 953, 1304
BspCNI CTCAG 4 cut(s) 102, 551, 562, 649
BspFNI CGCG 2 cut(s) 312, 314
BspHI TCATGA 2 cut(s) 417, 1081
BspLI GGNNCC 2 cut(s) 183, 584
BspMAI CTGCAG 1 cut(s) 1101
BspPI GGATC 2 cut(s) 1086, 1272
BspT107I GGYRCC 2 cut(s) 181, 582
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 3 cut(s) 157, 1012, 1305
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 369, 1025, 1270
BssHII GCGCGC 1 cut(s) 310
BssMI GATC 5 cut(s) 60, 752, 1018, 1078, 1277
BssNI GRCGYC 1 cut(s) 182
Bst2UI CCWGG 3 cut(s) 478, 1026, 1272
Bst4CI ACNGT 3 cut(s) 401, 701, 894
Bst6I CTCTTC 2 cut(s) 171, 940
BstACI GRCGYC 1 cut(s) 182
BstC8I GCNNGC 6 cut(s) 79, 312, 576, 769, 773, 1267
BstDEI CTNAG 7 cut(s) 89, 306, 538, 570, 657, 821, 1063
BstF5I GGATG 6 cut(s) 148, 152, 283, 400, 529, 922
BstFNI CGCG 2 cut(s) 312, 314
BstH2I RGCGCY 3 cut(s) 185, 937, 973
BstHHI GCGC 5 cut(s) 184, 312, 314, 936, 972
BstKTI GATC 5 cut(s) 63, 755, 1021, 1081, 1280
BstMBI GATC 5 cut(s) 60, 752, 1018, 1078, 1277
BstMWI GCNNNNNNNGC 3 cut(s) 311, 320, 967
BstNI CCWGG 3 cut(s) 478, 1026, 1272
BstNSI RCATGY 3 cut(s) 578, 1174, 1269
BstSCI CCNGG 4 cut(s) 368, 476, 1024, 1270
BstSFI CTRYAG 1 cut(s) 1097
BstUI CGCG 2 cut(s) 312, 314
BstV1I GCAGC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 752, 1277
BstXI CCANNNNNNTGG 1 cut(s) 484
BstYI RGATCY 2 cut(s) 752, 1277
BsuRI GGCC 3 cut(s) 53, 953, 1304
BtgZI GCGATG 2 cut(s) 329, 607
BtsCI GGATG 6 cut(s) 148, 152, 283, 400, 529, 922
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 6 cut(s) 79, 312, 576, 769, 773, 1267
CaiI CAGNNNCTG 1 cut(s) 843
CciI TCATGA 2 cut(s) 417, 1081
CfoI GCGC 5 cut(s) 184, 312, 314, 936, 972
Cfr10I RCCGGY 1 cut(s) 77
CviAII CATG 8 cut(s) 418, 575, 705, 736, 1051, 1082, 1171, 1266
DdeI CTNAG 7 cut(s) 89, 306, 538, 570, 657, 821, 1063
DinI GGCGCC 1 cut(s) 183
DpnI GATC 5 cut(s) 62, 754, 1020, 1080, 1279
DpnII GATC 5 cut(s) 60, 752, 1018, 1078, 1277
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 171, 940
EarI CTCTTC 2 cut(s) 171, 940
Eco147I AGGCCT 1 cut(s) 953
Eco47III AGCGCT 1 cut(s) 935
EcoRI GAATTC 1 cut(s) 472
EcoRII CCWGG 3 cut(s) 476, 1024, 1270
EgeI GGCGCC 1 cut(s) 183
EheI GGCGCC 1 cut(s) 183
FaeI CATG 8 cut(s) 421, 578, 708, 739, 1054, 1085, 1174, 1269
FalI AAGNNNNNCTT 2 cut(s) 847, 879
FaqI GGGAC 2 cut(s) 244, 357
FatI CATG 8 cut(s) 417, 574, 704, 735, 1050, 1081, 1170, 1265
FauI CCCGC 1 cut(s) 1353
FauNDI CATATG 1 cut(s) 556
FblI GTMKAC 1 cut(s) 1341
Fnu4HI GCNGC 1 cut(s) 324
FokI GGATG 6 cut(s) 135, 139, 290, 407, 536, 929
Fsp4HI GCNGC 1 cut(s) 324
FspBI CTAG 1 cut(s) 867
GlaI GCGC 5 cut(s) 183, 311, 313, 935, 971
GluI GCNGC 1 cut(s) 324
HaeII RGCGCY 3 cut(s) 185, 937, 973
HaeIII GGCC 3 cut(s) 53, 953, 1304
HapII CCGG 4 cut(s) 39, 50, 78, 369
HhaI GCGC 5 cut(s) 184, 312, 314, 936, 972
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 8 cut(s) 421, 578, 708, 739, 1054, 1085, 1174, 1269
Hin6I GCGC 5 cut(s) 182, 310, 312, 934, 970
HinP1I GCGC 5 cut(s) 182, 310, 312, 934, 970
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 6 cut(s) 113, 119, 267, 653, 1199, 1292
HpaII CCGG 4 cut(s) 39, 50, 78, 369
HphI GGTGA 2 cut(s) 422, 637
Hpy166II GTNNAC 3 cut(s) 166, 848, 1342
Hpy188I TCNGA 6 cut(s) 65, 118, 454, 571, 658, 1321
Hpy188III TCNNGA 4 cut(s) 271, 418, 1082, 1118
Hpy8I GTNNAC 3 cut(s) 166, 848, 1342
Hpy99I CGWCG 2 cut(s) 277, 1313
HpyAV CCTTC 3 cut(s) 95, 401, 892
HpyCH4III ACNGT 3 cut(s) 401, 701, 894
HpyCH4IV ACGT 3 cut(s) 777, 1308, 1316
HpyCH4V TGCA 9 cut(s) 125, 356, 560, 634, 704, 775, 1099, 1331, 1358
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 320, 967
HpyF3I CTNAG 7 cut(s) 89, 306, 538, 570, 657, 821, 1063
HpySE526I ACGT 3 cut(s) 777, 1308, 1316
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 8 cut(s) 421, 578, 708, 739, 1054, 1085, 1174, 1269
HspAI GCGC 5 cut(s) 182, 310, 312, 934, 970
KasI GGCGCC 1 cut(s) 181
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 5 cut(s) 60, 752, 1018, 1078, 1277
LmnI GCTCC 3 cut(s) 253, 904, 1112
Lsp1109I GCAGC 1 cut(s) 335
LweI GCATC 1 cut(s) 830
MaeI CTAG 1 cut(s) 867
MaeII ACGT 3 cut(s) 777, 1308, 1316
MaeIII GTNAC 1 cut(s) 643
MalI GATC 5 cut(s) 62, 754, 1020, 1080, 1279
MboI GATC 5 cut(s) 60, 752, 1018, 1078, 1277
MboII GAAGA 6 cut(s) 188, 493, 508, 855, 957, 1287
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 2 cut(s) 752, 1277
Mly113I GGCGCC 1 cut(s) 182
MlyI GAGTC 2 cut(s) 122, 662
MmeI TCCRAC 2 cut(s) 118, 1344
MnlI CCTC 9 cut(s) 127, 172, 196, 675, 826, 895, 943, 1291, 1330
MreI CGCCGGCG 1 cut(s) 77
MroNI GCCGGC 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 622
MseI TTAA 4 cut(s) 608, 1004, 1181, 1185
MspI CCGG 4 cut(s) 39, 50, 78, 369
MspR9I CCNGG 4 cut(s) 370, 478, 1026, 1272
MunI CAATTG 1 cut(s) 126
MvaI CCWGG 3 cut(s) 478, 1026, 1272
MvnI CGCG 2 cut(s) 312, 314
MwoI GCNNNNNNNGC 3 cut(s) 311, 320, 967
NaeI GCCGGC 1 cut(s) 79
NarI GGCGCC 1 cut(s) 182
NciI CCSGG 1 cut(s) 370
NdeI CATATG 1 cut(s) 556
NdeII GATC 5 cut(s) 60, 752, 1018, 1078, 1277
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 8 cut(s) 421, 578, 708, 739, 1054, 1085, 1174, 1269
NlaIV GGNNCC 2 cut(s) 183, 584
NmeAIII GCCGAG 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 643
NspI RCATGY 3 cut(s) 578, 1174, 1269
PacI TTAATTAA 1 cut(s) 1185
PaeI GCATGC 2 cut(s) 578, 1269
PagI TCATGA 2 cut(s) 417, 1081
PauI GCGCGC 1 cut(s) 310
PceI AGGCCT 1 cut(s) 953
PciI ACATGT 1 cut(s) 1170
PcsI WCGNNNNNNNCGW 1 cut(s) 1317
PdiI GCCGGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 4 cut(s) 119, 267, 1199, 1292
PfoI TCCNGGA 1 cut(s) 476
PkrI GCNGC 1 cut(s) 325
PleI GAGTC 2 cut(s) 121, 661
PluTI GGCGCC 1 cut(s) 185
PpsI GAGTC 2 cut(s) 121, 661
PscI ACATGT 1 cut(s) 1170
PsiI TTATAA 1 cut(s) 873
Psp6I CCWGG 3 cut(s) 476, 1024, 1270
PspGI CCWGG 3 cut(s) 476, 1024, 1270
PspN4I GGNNCC 2 cut(s) 183, 584
PstI CTGCAG 1 cut(s) 1101
PstNI CAGNNNCTG 1 cut(s) 843
PsuI RGATCY 2 cut(s) 752, 1277
PteI GCGCGC 1 cut(s) 310
SaqAI TTAA 4 cut(s) 608, 1004, 1181, 1185
SatI GCNGC 1 cut(s) 324
Sau3AI GATC 5 cut(s) 60, 752, 1018, 1078, 1277
SchI GAGTC 2 cut(s) 122, 662
ScrFI CCNGG 4 cut(s) 370, 478, 1026, 1272
SfaNI GCATC 1 cut(s) 830
SfcI CTRYAG 1 cut(s) 1097
SfoI GGCGCC 1 cut(s) 183
SgrAI CRCCGGYG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
SphI GCATGC 2 cut(s) 578, 1269
SseBI AGGCCT 1 cut(s) 953
SsiI CCGC 2 cut(s) 236, 1346
SspDI GGCGCC 1 cut(s) 181
SspI AATATT 1 cut(s) 877
SspMI CTAG 1 cut(s) 867
StuI AGGCCT 1 cut(s) 953
StyD4I CCNGG 4 cut(s) 368, 476, 1024, 1270
TaaI ACNGT 3 cut(s) 401, 701, 894
TaiI ACGT 3 cut(s) 780, 1311, 1319
TaqI TCGA 2 cut(s) 272, 1311
TfiI GAWTC 4 cut(s) 119, 267, 1199, 1292
Tru1I TTAA 4 cut(s) 608, 1004, 1181, 1185
Tru9I TTAA 4 cut(s) 608, 1004, 1181, 1185
TscAI CASTG 1 cut(s) 469
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 1 cut(s) 643
TspDTI ATGAA 9 cut(s) 137, 434, 540, 543, 707, 933, 953, 1067, 1098
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 1 cut(s) 469
XapI RAATTY 2 cut(s) 105, 472
XceI RCATGY 3 cut(s) 578, 1174, 1269
XmiI GTMKAC 1 cut(s) 1341
XmnI GAANNNNTTC 1 cut(s) 622
XspI CTAG 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.