RchiOBHm_Chr3g0449661

Protein prenyltransferase alpha subunit repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001731
Physical Location & Seq
Reverse (-)
1357702 .. 1361578
3877 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41702

Sequence Viewer

Length: 1398 bp
ATGAGATGGTATAATAAGCCAATTGATTCGCATTCTCTGATTGACTGTGCCTCTTCTACTCCGACGATTTTCCGGCGAAAGATGAACGAAGAAGAAAAGGCCGGCGAGCTTCTGAATCAGTTCGAACAGATTTTGGAGTCCGATCCTGCAATTGATGAGATTGGATTTGTTCATCCATCCCTGTTTGTCAACTTGAATGAAGAGGCATCCTCATCATCTGATGGTATTAGTAATGTGGTGTGCTCAGCAGATGGGAGTGCAGTGTTTTGGAATAGGGATCATAAGTTGGGAATTTCGACGGATGTTCTTATTCCATTATATAAGGAAGCTAAGCGCGCGCTGATGGCAGCAAATCGACAATATAAGGGAGTTTGTAGCACAGCTGATATAACTGGGGTTGAGAGTTCTGTATGTGACTCATTATCGTTGGGTAACGGTGTTGAAAGTGAAGTCATGAAGCATAGCAGGGCTGTTTTGTTAGTGAGTTCTGATTTTGGCACTGCCTGGAATTCCAGGAAGATGGTGTTGTTAAAGAAGCAAGAGCTCTCAATATTATTGGATGAACTTCTATTCTCAGCTCTTGTTCTTTCATATGCACCTAAATCTGAGCATGCTTGGAGCCACAGGCGATGGGTCATCAAGTTAATTGCTGGAAAGTGTTCAACTCTGCAAGAGATTGTCACCAAAGAGTCTGAGCTGGTGGAGAAAATAGCTGAGAGGTCAAAAATGAATTACCGCGCATGGTATCATCGTTGCTGGTTGGTGACCTACATGACAACAGAGCTGTCTCTACATGAATTAAAGAGATCTAAAAACTGGGCGGGCTTGCATGTTGCTGACCATAGTTGCTTCCATTATCGCAGACAGCTAATGCTTAGGATTTTAGAGGACAGATGCTGTAAACAAGAAAATGAATCTTCTAGTTGTAGTGTTAAAATAAATCAATTATGGAAGGAGGAGATTGATTGGAATGAAATGTTGATAAAGCGCTACATAGGAAGAGAGGCTCTATGGCTTCATCGGCGCTTCCTATCCTTATTTTGGATAAAACATTTTTTAACTGGTCAGACCAGTCTGCCTGGGGATTGGAAGCACAAAACCAGCATGAATAATGACTTTGGTATCTTTGTGGATAATGAGTTACATCTCCTGCAGTCCTGCTCCATCATTCCAGATGATAGTTTTGATGATTACCAAGCACAGGCTACATATTCTGCTACTTACATGTTATGGTTGATTAAGCAAATCCCTGATTCTGGTGGCGTTGAATTTTTTGAGAAGCTAAGAACCGAACACCTGAATGCCCTGCTGAACAAGGCCTGCCCTGAAAGGTCCTCAACTTGGGATTCTATAATGGCCTATGTCGAAACATCCGACCATTATGTGAAAGAGAGTTAG

Protein Analysis

465

Amino Acids

53.53

Weight (kDa)

6.12

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPTA PF01239 186 - 214 1.5e-06 Protein prenyltransferase alpha subunit repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 336, 338, 738
AciI CCGC 2 cut(s) 736, 821
AclWI GGATC 2 cut(s) 137, 285
AcsI RAATTY 3 cut(s) 291, 508, 1268
AfeI AGCGCT 1 cut(s) 989
AfiI CCNNNNNNNGG 4 cut(s) 1041, 1201, 1256, 1341
AflIII ACRYGT 1 cut(s) 1224
AgsI TTSAA 4 cut(s) 196, 443, 663, 1268
AjnI CCWGG 3 cut(s) 503, 512, 1078
Alw21I GWGCWC 2 cut(s) 245, 546
Alw26I GTCTC 1 cut(s) 792
AlwI GGATC 2 cut(s) 137, 285
AlwNI CAGNNNCTG 1 cut(s) 897
Aor51HI AGCGCT 1 cut(s) 989
AoxI GGCC 3 cut(s) 99, 1317, 1356
ApeKI GCWGC 1 cut(s) 347
ApoI RAATTY 3 cut(s) 291, 508, 1268
Asp700I GAANNNNTTC 2 cut(s) 119, 658
AspLEI GCGC 6 cut(s) 336, 338, 340, 740, 990, 1026
AspS9I GGNCC 1 cut(s) 1332
AsuHPI GGTGA 2 cut(s) 673, 775
AsuII TTCGAA 1 cut(s) 123
AvaII GGWCC 1 cut(s) 1332
BaeI ACNNNNGTAYC 2 cut(s) 1105, 1138
BanII GRGCYC 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 245, 546
BbvI GCAGC 1 cut(s) 359
BccI CCATC 7 cut(s) 184, 215, 245, 337, 514, 624, 1172
BciT130I CCWGG 3 cut(s) 505, 514, 1080
BcoDI GTCTC 1 cut(s) 792
BfaI CTAG 1 cut(s) 921
BfmI CTRYAG 1 cut(s) 1151
BfoI RGCGCY 2 cut(s) 991, 1027
BglII AGATCT 1 cut(s) 806
BisI GCNGC 1 cut(s) 348
BlpI GCTNAGC 2 cut(s) 244, 330
BlsI GCNGC 1 cut(s) 349
Bme1390I CCNGG 3 cut(s) 505, 514, 1080
Bme18I GGWCC 1 cut(s) 1332
BmgT120I GGNCC 1 cut(s) 1332
BmiI GGNNCC 1 cut(s) 620
BmrFI CCNGG 3 cut(s) 505, 514, 1080
BmrI ACTGGG 2 cut(s) 402, 826
BmsI GCATC 2 cut(s) 215, 884
BmuI ACTGGG 2 cut(s) 402, 826
BplI GAGNNNNNCTC 2 cut(s) 194, 226
Bpu10I CCTNAGC 1 cut(s) 875
Bpu1102I GCTNAGC 2 cut(s) 244, 330
Bpu14I TTCGAA 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 1079
Bsc4I CCNNNNNNNGG 4 cut(s) 1041, 1201, 1256, 1341
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 4 cut(s) 397, 821, 1066, 1071
BseBI CCWGG 3 cut(s) 505, 514, 1080
BseDI CCNNGG 1 cut(s) 1079
BseGI GGATG 6 cut(s) 172, 176, 206, 307, 565, 1370
BseLI CCNNNNNNNGG 4 cut(s) 1041, 1201, 1256, 1341
BseMII CTCAG 5 cut(s) 258, 588, 597, 684, 705
BseNI ACTGG 4 cut(s) 397, 821, 1066, 1071
BsePI GCGCGC 2 cut(s) 334, 336
BseRI GAGGAG 1 cut(s) 971
BseXI GCAGC 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 279
Bsh1236I CGCG 3 cut(s) 336, 338, 738
BshFI GGCC 3 cut(s) 101, 1319, 1358
BsiHKAI GWGCWC 2 cut(s) 245, 546
BsiSI CCGG 2 cut(s) 73, 102
BslI CCNNNNNNNGG 4 cut(s) 1041, 1201, 1256, 1341
BsmAI GTCTC 1 cut(s) 792
BsmI GAATGC 2 cut(s) 31, 1306
BsnI GGCC 3 cut(s) 101, 1319, 1358
Bsp119I TTCGAA 1 cut(s) 123
Bsp1286I GDGCHC 2 cut(s) 245, 546
Bsp143I GATC 3 cut(s) 142, 277, 806
Bsp1720I GCTNAGC 2 cut(s) 244, 330
BspACI CCGC 2 cut(s) 736, 821
BspANI GGCC 3 cut(s) 101, 1319, 1358
BspCNI CTCAG 5 cut(s) 257, 587, 598, 685, 706
BspFNI CGCG 3 cut(s) 336, 338, 738
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 1 cut(s) 620
BspMAI CTGCAG 1 cut(s) 1155
BspPI GGATC 2 cut(s) 137, 285
BspT104I TTCGAA 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 4 cut(s) 397, 821, 1066, 1071
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 1079
BssHII GCGCGC 2 cut(s) 334, 336
BssMI GATC 3 cut(s) 142, 277, 806
Bst2UI CCWGG 3 cut(s) 505, 514, 1080
Bst4CI ACNGT 2 cut(s) 47, 437
Bst6I CTCTTC 3 cut(s) 58, 195, 994
BstBI TTCGAA 1 cut(s) 123
BstC8I GCNNGC 8 cut(s) 103, 107, 336, 338, 612, 823, 827, 1321
BstDEI CTNAG 8 cut(s) 244, 330, 574, 606, 693, 714, 875, 1283
BstEII GGTNACC 1 cut(s) 763
BstF5I GGATG 6 cut(s) 172, 176, 206, 307, 565, 1370
BstFNI CGCG 3 cut(s) 336, 338, 738
BstH2I RGCGCY 2 cut(s) 991, 1027
BstHHI GCGC 6 cut(s) 336, 338, 340, 740, 990, 1026
BstKTI GATC 3 cut(s) 145, 280, 809
BstMAI GTCTC 1 cut(s) 792
BstMBI GATC 3 cut(s) 142, 277, 806
BstMWI GCNNNNNNNGC 3 cut(s) 335, 344, 1021
BstNI CCWGG 3 cut(s) 505, 514, 1080
BstNSI RCATGY 3 cut(s) 614, 833, 1228
BstPI GGTNACC 1 cut(s) 763
BstSCI CCNGG 3 cut(s) 503, 512, 1078
BstSFI CTRYAG 1 cut(s) 1151
BstUI CGCG 3 cut(s) 336, 338, 738
BstV1I GCAGC 1 cut(s) 359
BstX2I RGATCY 1 cut(s) 806
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 806
BsuRI GGCC 3 cut(s) 101, 1319, 1358
BtgZI GCGATG 1 cut(s) 643
BtsCI GGATG 6 cut(s) 172, 176, 206, 307, 565, 1370
BtsI GCAGTG 2 cut(s) 267, 498
BtsIMutI CAGTG 2 cut(s) 267, 498
Cac8I GCNNGC 8 cut(s) 103, 107, 336, 338, 612, 823, 827, 1321
CaiI CAGNNNCTG 1 cut(s) 897
CciI TCATGA 1 cut(s) 453
CfoI GCGC 6 cut(s) 336, 338, 340, 740, 990, 1026
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 1332
CviAII CATG 8 cut(s) 454, 611, 741, 772, 794, 830, 1105, 1225
DdeI CTNAG 8 cut(s) 244, 330, 574, 606, 693, 714, 875, 1283
DpnI GATC 3 cut(s) 144, 279, 808
DpnII GATC 3 cut(s) 142, 277, 806
Eam1104I CTCTTC 3 cut(s) 58, 195, 994
EarI CTCTTC 3 cut(s) 58, 195, 994
Ecl136II GAGCTC 1 cut(s) 544
Eco147I AGGCCT 1 cut(s) 1319
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 1332
Eco47III AGCGCT 1 cut(s) 989
Eco53kI GAGCTC 1 cut(s) 544
Eco91I GGTNACC 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 1332
EcoO65I GGTNACC 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 508
EcoRII CCWGG 3 cut(s) 503, 512, 1078
EcoT38I GRGCYC 1 cut(s) 546
FaeI CATG 8 cut(s) 457, 614, 744, 775, 797, 833, 1108, 1228
FatI CATG 8 cut(s) 453, 610, 740, 771, 793, 829, 1104, 1224
FauI CCCGC 1 cut(s) 814
FauNDI CATATG 1 cut(s) 592
Fnu4HI GCNGC 1 cut(s) 348
FokI GGATG 6 cut(s) 159, 163, 193, 314, 572, 1357
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 1 cut(s) 348
FspBI CTAG 1 cut(s) 921
GlaI GCGC 6 cut(s) 335, 337, 339, 739, 989, 1025
GluI GCNGC 1 cut(s) 348
HaeII RGCGCY 2 cut(s) 991, 1027
HaeIII GGCC 3 cut(s) 101, 1319, 1358
HapII CCGG 2 cut(s) 73, 102
HhaI GCGC 6 cut(s) 336, 338, 340, 740, 990, 1026
Hin1II CATG 8 cut(s) 457, 614, 744, 775, 797, 833, 1108, 1228
Hin6I GCGC 6 cut(s) 334, 336, 338, 738, 988, 1024
HinP1I GCGC 6 cut(s) 334, 336, 338, 738, 988, 1024
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 8 cut(s) 26, 115, 137, 416, 689, 914, 1253, 1346
HpaII CCGG 2 cut(s) 73, 102
HphI GGTGA 2 cut(s) 673, 775
Hpy166II GTNNAC 2 cut(s) 190, 902
Hpy188III TCNNGA 2 cut(s) 454, 1172
Hpy8I GTNNAC 2 cut(s) 190, 902
Hpy99I CGWCG 2 cut(s) 67, 301
HpyAV CCTTC 1 cut(s) 946
HpyCH4III ACNGT 2 cut(s) 47, 437
HpyCH4V TGCA 6 cut(s) 149, 260, 596, 670, 829, 1153
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 344, 1021
HpyF3I CTNAG 8 cut(s) 244, 330, 574, 606, 693, 714, 875, 1283
Hsp92II CATG 8 cut(s) 457, 614, 744, 775, 797, 833, 1108, 1228
HspAI GCGC 6 cut(s) 334, 336, 338, 738, 988, 1024
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Kzo9I GATC 3 cut(s) 142, 277, 806
LmnI GCTCC 2 cut(s) 618, 1166
Lsp1109I GCAGC 1 cut(s) 359
LweI GCATC 2 cut(s) 215, 884
MaeI CTAG 1 cut(s) 921
MaeIII GTNAC 5 cut(s) 413, 431, 679, 763, 1140
MalI GATC 3 cut(s) 144, 279, 808
MboI GATC 3 cut(s) 142, 277, 806
MboII GAAGA 7 cut(s) 45, 101, 104, 212, 529, 909, 1011
MfeI CAATTG 2 cut(s) 21, 150
MflI RGATCY 1 cut(s) 806
MhlI GDGCHC 2 cut(s) 245, 546
MluCI AATT 9 cut(s) 21, 150, 291, 508, 645, 730, 797, 944, 1268
MlyI GAGTC 3 cut(s) 146, 410, 698
MmeI TCCRAC 2 cut(s) 86, 1398
MnlI CCTC 8 cut(s) 61, 196, 220, 711, 880, 949, 997, 1345
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 2 cut(s) 119, 658
MseI TTAA 6 cut(s) 530, 644, 800, 933, 1058, 1239
MslI CAYNNNNRTG 1 cut(s) 1299
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 73, 102
MspR9I CCNGG 3 cut(s) 505, 514, 1080
MunI CAATTG 2 cut(s) 21, 150
Mva1269I GAATGC 2 cut(s) 31, 1306
MvaI CCWGG 3 cut(s) 505, 514, 1080
MvnI CGCG 3 cut(s) 336, 338, 738
MwoI GCNNNNNNNGC 3 cut(s) 335, 344, 1021
NaeI GCCGGC 1 cut(s) 103
NdeI CATATG 1 cut(s) 592
NdeII GATC 3 cut(s) 142, 277, 806
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 8 cut(s) 457, 614, 744, 775, 797, 833, 1108, 1228
NlaIV GGNNCC 1 cut(s) 620
NmuCI GTSAC 3 cut(s) 413, 679, 763
NspI RCATGY 3 cut(s) 614, 833, 1228
NspV TTCGAA 1 cut(s) 123
PaeI GCATGC 1 cut(s) 614
PagI TCATGA 1 cut(s) 453
PauI GCGCGC 2 cut(s) 334, 336
PceI AGGCCT 1 cut(s) 1319
PciI ACATGT 1 cut(s) 1224
PcsI WCGNNNNNNNCGW 1 cut(s) 1371
PctI GAATGC 2 cut(s) 31, 1306
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 2 cut(s) 119, 658
PfeI GAWTC 5 cut(s) 26, 115, 914, 1253, 1346
PfoI TCCNGGA 1 cut(s) 512
PkrI GCNGC 1 cut(s) 349
PleI GAGTC 3 cut(s) 145, 410, 697
PpsI GAGTC 3 cut(s) 145, 410, 697
PpuMI RGGWCCY 1 cut(s) 1332
PscI ACATGT 1 cut(s) 1224
Psp124BI GAGCTC 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 1332
Psp6I CCWGG 3 cut(s) 503, 512, 1078
PspEI GGTNACC 1 cut(s) 763
PspGI CCWGG 3 cut(s) 503, 512, 1078
PspN4I GGNNCC 1 cut(s) 620
PspPI GGNCC 1 cut(s) 1332
PspPPI RGGWCCY 1 cut(s) 1332
PstI CTGCAG 1 cut(s) 1155
PstNI CAGNNNCTG 1 cut(s) 897
PsuI RGATCY 1 cut(s) 806
PteI GCGCGC 2 cut(s) 334, 336
PvuII CAGCTG 1 cut(s) 383
RseI CAYNNNNRTG 1 cut(s) 1299
SacI GAGCTC 1 cut(s) 546
SaqAI TTAA 6 cut(s) 530, 644, 800, 933, 1058, 1239
SatI GCNGC 1 cut(s) 348
Sau3AI GATC 3 cut(s) 142, 277, 806
Sau96I GGNCC 1 cut(s) 1332
SchI GAGTC 3 cut(s) 146, 410, 698
ScrFI CCNGG 3 cut(s) 505, 514, 1080
SduI GDGCHC 2 cut(s) 245, 546
SfaNI GCATC 2 cut(s) 215, 884
SfcI CTRYAG 1 cut(s) 1151
SfuI TTCGAA 1 cut(s) 123
SinI GGWCC 1 cut(s) 1332
SmiMI CAYNNNNRTG 1 cut(s) 1299
SphI GCATGC 1 cut(s) 614
Sse9I AATT 9 cut(s) 21, 150, 291, 508, 645, 730, 797, 944, 1268
SseBI AGGCCT 1 cut(s) 1319
SsiI CCGC 2 cut(s) 736, 821
SspI AATATT 1 cut(s) 552
SspMI CTAG 1 cut(s) 921
SstI GAGCTC 1 cut(s) 546
StuI AGGCCT 1 cut(s) 1319
StyD4I CCNGG 3 cut(s) 503, 512, 1078
TaaI ACNGT 2 cut(s) 47, 437
TaqI TCGA 4 cut(s) 123, 296, 355, 1365
TasI AATT 9 cut(s) 21, 150, 291, 508, 645, 730, 797, 944, 1268
TfiI GAWTC 5 cut(s) 26, 115, 914, 1253, 1346
Tru1I TTAA 6 cut(s) 530, 644, 800, 933, 1058, 1239
Tru9I TTAA 6 cut(s) 530, 644, 800, 933, 1058, 1239
TscAI CASTG 2 cut(s) 267, 505
TseFI GTSAC 3 cut(s) 413, 679, 763
TseI GCWGC 1 cut(s) 347
Tsp45I GTSAC 3 cut(s) 413, 679, 763
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 2 cut(s) 267, 505
VpaK11BI GGWCC 1 cut(s) 1332
XapI RAATTY 3 cut(s) 291, 508, 1268
XceI RCATGY 3 cut(s) 614, 833, 1228
XmnI GAANNNNTTC 2 cut(s) 119, 658
XspI CTAG 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.