Rh3BG021400

Protein prenyltransferase alpha subunit repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001731
Physical Location & Seq
Reverse (-)
1350684 .. 1354297
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG021400.1

Sequence Viewer

Length: 1287 bp
ATGCAAGTGGCTTATGGTTTGTGTTTTGCACTTGAACTCAGTGATGAGATTGGATTTGTTCATCCATCCCTGTTTGTCAACTTGAATGAAGAGGCATCCTCATCATCTGATGGTATTAGTAATGTGGTGTGCTCAGCAGATGGGAGTGCAGTGTTTTGGAATAGGGATCATAAGTTGGGAATTTCGACGGATGTTCTTATTCCATTATATAAGGAAGCTAAGCGCGCGCTGATGGCAGCAAATCGACAATATAAGGGACTTTGTAGCACATCTGATATAACTGGGGTTGAGAGTTCTGTATGTGACTCATTGTTGTTGGGTAACGGTGTTGAAAGTGAAGTCATGAAGCATAGCAGGGCTGTTTTGGTAGTGAGTTCTGATTTTGGCACTGCTTGGCATTCCAGGAAGATGGTGTTGTTAAAGAAGCAAGAGCTCTCAATATTATTGGATGAACTTCTATTCTCAGCTCTTGTTCTTTCATATGCACCTAAATCTGAGCATGCTTGGAGCCACAGGCGATGGGTCATCAAGTTAATTGCTGGAAAGTGTTCAACTCTGCAAGAGATTGTCACCAAAGAGTCTGAGCTGGTGGAGAAAATAGCTGAGAGGTCAAAAATGAATTACCGCGCATGGTATCATCGTTGCTGGTTGGTGACCTACATGACAACAGAGCTGTCTCTACATGAATTAAAGAGATCTAAAAACTGGGCGGGCTTGCATGTTGCTGACCATAGTTGCTTCCATTATCGCAGACAGCTAATGCTTAGGATTTTAGAGGACAGATGCTGTAAACAAGAAAATGAATCTTCTAGTTATAGTGTTAAAATAAATCAATTATGGAAGGAGGAGCTTGATTGGAATGAAATGTTGATAAAGCGCTACATAGGAAGAGAGGCTCTATGGCTTCATCGGCGCTTCCTATCCTTATTTTGGATAAAACATTTTTTAACTGGTCAGACCAGTCTGCCTGGGGATTGGAAGCACAAAACCAGCATGAATAATGACTTTGGTATCTTTGTGGATAATGAGTTACATCTCCTGCAGTCCTGCTCCATCATTCCAGATGATAGTTTTGATGATTACCAAGCACAGGCTACATATTCTGCTACTTACATGTTATGGTTGATTAAGCAAATCCCTGATTCTGGTGGCGTTGAATTTCTTGAGAAGCTAAGAACCAAACACCTGAATGCCCTGCTGAACAAGGCCTGCCCTGAAAGGTCCTCAACTTGGGATTCTATACTGGCCTATGTCGAAACATCCGACCATTATGTGAAAGAGAGTTAG

Protein Analysis

428

Amino Acids

49.19

Weight (kDa)

6.5

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPTA PF01239 149 - 177 1.4e-06 Protein prenyltransferase alpha subunit repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 225, 227, 627
AciI CCGC 2 cut(s) 625, 710
AclWI GGATC 1 cut(s) 174
AcsI RAATTY 2 cut(s) 180, 1157
AfeI AGCGCT 1 cut(s) 878
AfiI CCNNNNNNNGG 4 cut(s) 930, 1090, 1145, 1230
AflIII ACRYGT 1 cut(s) 1113
AgsI TTSAA 5 cut(s) 35, 85, 332, 552, 1157
AjnI CCWGG 2 cut(s) 401, 967
AluBI AGCT 9 cut(s) 218, 433, 467, 586, 602, 673, 757, 850, 1171
AluI AGCT 9 cut(s) 218, 433, 467, 586, 602, 673, 757, 850, 1171
Alw21I GWGCWC 2 cut(s) 134, 435
Alw26I GTCTC 1 cut(s) 681
AlwI GGATC 1 cut(s) 174
AlwNI CAGNNNCTG 1 cut(s) 786
Aor51HI AGCGCT 1 cut(s) 878
AoxI GGCC 2 cut(s) 1206, 1245
ApeKI GCWGC 1 cut(s) 236
ApoI RAATTY 2 cut(s) 180, 1157
Asp700I GAANNNNTTC 1 cut(s) 547
AspLEI GCGC 6 cut(s) 225, 227, 229, 629, 879, 915
AspS9I GGNCC 1 cut(s) 1221
AsuHPI GGTGA 2 cut(s) 562, 664
AvaII GGWCC 1 cut(s) 1221
BaeI ACNNNNGTAYC 2 cut(s) 994, 1027
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 2 cut(s) 134, 435
BbvI GCAGC 1 cut(s) 248
BccI CCATC 7 cut(s) 73, 104, 134, 226, 403, 513, 1061
BciT130I CCWGG 2 cut(s) 403, 969
BcoDI GTCTC 1 cut(s) 681
BfaI CTAG 1 cut(s) 810
BfmI CTRYAG 1 cut(s) 1040
BfoI RGCGCY 2 cut(s) 880, 916
BglII AGATCT 1 cut(s) 695
BisI GCNGC 1 cut(s) 237
BlpI GCTNAGC 2 cut(s) 133, 219
BlsI GCNGC 1 cut(s) 238
Bme1390I CCNGG 2 cut(s) 403, 969
Bme18I GGWCC 1 cut(s) 1221
BmgT120I GGNCC 1 cut(s) 1221
BmiI GGNNCC 1 cut(s) 509
BmrFI CCNGG 2 cut(s) 403, 969
BmrI ACTGGG 2 cut(s) 291, 715
BmsI GCATC 2 cut(s) 104, 773
BmuI ACTGGG 2 cut(s) 291, 715
BplI GAGNNNNNCTC 2 cut(s) 83, 115
Bpu10I CCTNAGC 1 cut(s) 764
Bpu1102I GCTNAGC 2 cut(s) 133, 219
BpuEI CTTGAG 1 cut(s) 1184
BsaJI CCNNGG 1 cut(s) 968
Bsc4I CCNNNNNNNGG 4 cut(s) 930, 1090, 1145, 1230
Bse1I ACTGG 5 cut(s) 286, 710, 955, 960, 1248
BseBI CCWGG 2 cut(s) 403, 969
BseDI CCNNGG 1 cut(s) 968
BseGI GGATG 6 cut(s) 61, 65, 95, 196, 454, 1259
BseLI CCNNNNNNNGG 4 cut(s) 930, 1090, 1145, 1230
BseMII CTCAG 6 cut(s) 52, 147, 477, 486, 573, 594
BseNI ACTGG 5 cut(s) 286, 710, 955, 960, 1248
BsePI GCGCGC 2 cut(s) 223, 225
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 168
Bsh1236I CGCG 3 cut(s) 225, 227, 627
BshFI GGCC 2 cut(s) 1208, 1247
BsiHKAI GWGCWC 2 cut(s) 134, 435
BslFI GGGAC 1 cut(s) 270
BslI CCNNNNNNNGG 4 cut(s) 930, 1090, 1145, 1230
BsmAI GTCTC 1 cut(s) 681
BsmFI GGGAC 1 cut(s) 270
BsmI GAATGC 2 cut(s) 397, 1195
BsnI GGCC 2 cut(s) 1208, 1247
Bsp1286I GDGCHC 2 cut(s) 134, 435
Bsp143I GATC 2 cut(s) 166, 695
Bsp1720I GCTNAGC 2 cut(s) 133, 219
BspACI CCGC 2 cut(s) 625, 710
BspANI GGCC 2 cut(s) 1208, 1247
BspCNI CTCAG 6 cut(s) 51, 146, 476, 487, 574, 595
BspFNI CGCG 3 cut(s) 225, 227, 627
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 509
BspMAI CTGCAG 1 cut(s) 1044
BspPI GGATC 1 cut(s) 174
BsrI ACTGG 5 cut(s) 286, 710, 955, 960, 1248
BssECI CCNNGG 1 cut(s) 968
BssHII GCGCGC 2 cut(s) 223, 225
BssMI GATC 2 cut(s) 166, 695
Bst2UI CCWGG 2 cut(s) 403, 969
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 2 cut(s) 84, 883
BstC8I GCNNGC 6 cut(s) 225, 227, 501, 712, 716, 1210
BstDEI CTNAG 9 cut(s) 38, 133, 219, 463, 495, 582, 603, 764, 1172
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 6 cut(s) 61, 65, 95, 196, 454, 1259
BstFNI CGCG 3 cut(s) 225, 227, 627
BstH2I RGCGCY 2 cut(s) 880, 916
BstHHI GCGC 6 cut(s) 225, 227, 229, 629, 879, 915
BstKTI GATC 2 cut(s) 169, 698
BstMAI GTCTC 1 cut(s) 681
BstMBI GATC 2 cut(s) 166, 695
BstMWI GCNNNNNNNGC 3 cut(s) 224, 233, 910
BstNI CCWGG 2 cut(s) 403, 969
BstNSI RCATGY 3 cut(s) 503, 722, 1117
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 2 cut(s) 401, 967
BstSFI CTRYAG 1 cut(s) 1040
BstUI CGCG 3 cut(s) 225, 227, 627
BstV1I GCAGC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 695
BstXI CCANNNNNNTGG 1 cut(s) 409
BstYI RGATCY 1 cut(s) 695
BsuRI GGCC 2 cut(s) 1208, 1247
BtgZI GCGATG 1 cut(s) 532
BtsCI GGATG 6 cut(s) 61, 65, 95, 196, 454, 1259
BtsI GCAGTG 2 cut(s) 156, 387
BtsIMutI CAGTG 3 cut(s) 46, 156, 387
Cac8I GCNNGC 6 cut(s) 225, 227, 501, 712, 716, 1210
CaiI CAGNNNCTG 1 cut(s) 786
CciI TCATGA 1 cut(s) 342
CfoI GCGC 6 cut(s) 225, 227, 229, 629, 879, 915
Cfr13I GGNCC 1 cut(s) 1221
CviAII CATG 8 cut(s) 343, 500, 630, 661, 683, 719, 994, 1114
DdeI CTNAG 9 cut(s) 38, 133, 219, 463, 495, 582, 603, 764, 1172
DpnI GATC 2 cut(s) 168, 697
DpnII GATC 2 cut(s) 166, 695
Eam1104I CTCTTC 2 cut(s) 84, 883
EarI CTCTTC 2 cut(s) 84, 883
Ecl136II GAGCTC 1 cut(s) 433
Eco147I AGGCCT 1 cut(s) 1208
Eco24I GRGCYC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 1221
Eco47III AGCGCT 1 cut(s) 878
Eco53kI GAGCTC 1 cut(s) 433
Eco91I GGTNACC 1 cut(s) 652
EcoICRI GAGCTC 1 cut(s) 433
EcoO109I RGGNCCY 1 cut(s) 1221
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 401, 967
EcoT38I GRGCYC 1 cut(s) 435
FaeI CATG 8 cut(s) 346, 503, 633, 664, 686, 722, 997, 1117
FaqI GGGAC 1 cut(s) 270
FatI CATG 8 cut(s) 342, 499, 629, 660, 682, 718, 993, 1113
FauI CCCGC 1 cut(s) 703
FauNDI CATATG 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 237
FokI GGATG 6 cut(s) 48, 52, 82, 203, 461, 1246
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 1 cut(s) 237
FspBI CTAG 1 cut(s) 810
GlaI GCGC 6 cut(s) 224, 226, 228, 628, 878, 914
GluI GCNGC 1 cut(s) 237
HaeII RGCGCY 2 cut(s) 880, 916
HaeIII GGCC 2 cut(s) 1208, 1247
HhaI GCGC 6 cut(s) 225, 227, 229, 629, 879, 915
Hin1II CATG 8 cut(s) 346, 503, 633, 664, 686, 722, 997, 1117
Hin6I GCGC 6 cut(s) 223, 225, 227, 627, 877, 913
HinP1I GCGC 6 cut(s) 223, 225, 227, 627, 877, 913
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 5 cut(s) 305, 578, 803, 1142, 1235
HphI GGTGA 2 cut(s) 562, 664
Hpy166II GTNNAC 2 cut(s) 79, 791
Hpy188I TCNGA 7 cut(s) 109, 274, 379, 496, 583, 957, 1264
Hpy188III TCNNGA 3 cut(s) 343, 1061, 1163
Hpy8I GTNNAC 2 cut(s) 79, 791
Hpy99I CGWCG 1 cut(s) 190
HpyAV CCTTC 1 cut(s) 835
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4V TGCA 7 cut(s) 4, 29, 149, 485, 559, 718, 1042
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 233, 910
HpyF3I CTNAG 9 cut(s) 38, 133, 219, 463, 495, 582, 603, 764, 1172
Hsp92II CATG 8 cut(s) 346, 503, 633, 664, 686, 722, 997, 1117
HspAI GCGC 6 cut(s) 223, 225, 227, 627, 877, 913
Kzo9I GATC 2 cut(s) 166, 695
LmnI GCTCC 3 cut(s) 507, 847, 1055
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 2 cut(s) 104, 773
MaeI CTAG 1 cut(s) 810
MaeIII GTNAC 5 cut(s) 302, 320, 568, 652, 1029
MalI GATC 2 cut(s) 168, 697
MboI GATC 2 cut(s) 166, 695
MboII GAAGA 4 cut(s) 101, 418, 798, 900
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 134, 435
MluCI AATT 6 cut(s) 180, 534, 619, 686, 833, 1157
MlyI GAGTC 2 cut(s) 299, 587
MmeI TCCRAC 1 cut(s) 1287
MnlI CCTC 7 cut(s) 85, 109, 600, 769, 838, 886, 1234
MroXI GAANNNNTTC 1 cut(s) 547
MseI TTAA 6 cut(s) 419, 533, 689, 822, 947, 1128
MslI CAYNNNNRTG 1 cut(s) 1188
MspR9I CCNGG 2 cut(s) 403, 969
Mva1269I GAATGC 2 cut(s) 397, 1195
MvaI CCWGG 2 cut(s) 403, 969
MvnI CGCG 3 cut(s) 225, 227, 627
MwoI GCNNNNNNNGC 3 cut(s) 224, 233, 910
NdeI CATATG 1 cut(s) 481
NdeII GATC 2 cut(s) 166, 695
NlaIII CATG 8 cut(s) 346, 503, 633, 664, 686, 722, 997, 1117
NlaIV GGNNCC 1 cut(s) 509
NmuCI GTSAC 3 cut(s) 302, 568, 652
NspI RCATGY 3 cut(s) 503, 722, 1117
PaeI GCATGC 1 cut(s) 503
PagI TCATGA 1 cut(s) 342
PauI GCGCGC 2 cut(s) 223, 225
PceI AGGCCT 1 cut(s) 1208
PciI ACATGT 1 cut(s) 1113
PcsI WCGNNNNNNNCGW 1 cut(s) 1260
PctI GAATGC 2 cut(s) 397, 1195
PdmI GAANNNNTTC 1 cut(s) 547
PfeI GAWTC 3 cut(s) 803, 1142, 1235
PfoI TCCNGGA 1 cut(s) 401
PkrI GCNGC 1 cut(s) 238
PleI GAGTC 2 cut(s) 299, 586
PpsI GAGTC 2 cut(s) 299, 586
PpuMI RGGWCCY 1 cut(s) 1221
PscI ACATGT 1 cut(s) 1113
Psp124BI GAGCTC 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 1221
Psp6I CCWGG 2 cut(s) 401, 967
PspEI GGTNACC 1 cut(s) 652
PspGI CCWGG 2 cut(s) 401, 967
PspN4I GGNNCC 1 cut(s) 509
PspPI GGNCC 1 cut(s) 1221
PspPPI RGGWCCY 1 cut(s) 1221
PstI CTGCAG 1 cut(s) 1044
PstNI CAGNNNCTG 1 cut(s) 786
PsuI RGATCY 1 cut(s) 695
PteI GCGCGC 2 cut(s) 223, 225
RseI CAYNNNNRTG 1 cut(s) 1188
SacI GAGCTC 1 cut(s) 435
SaqAI TTAA 6 cut(s) 419, 533, 689, 822, 947, 1128
SatI GCNGC 1 cut(s) 237
Sau3AI GATC 2 cut(s) 166, 695
Sau96I GGNCC 1 cut(s) 1221
SchI GAGTC 2 cut(s) 299, 587
ScrFI CCNGG 2 cut(s) 403, 969
SduI GDGCHC 2 cut(s) 134, 435
SfaNI GCATC 2 cut(s) 104, 773
SfcI CTRYAG 1 cut(s) 1040
SinI GGWCC 1 cut(s) 1221
SmiMI CAYNNNNRTG 1 cut(s) 1188
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
SphI GCATGC 1 cut(s) 503
Sse9I AATT 6 cut(s) 180, 534, 619, 686, 833, 1157
SseBI AGGCCT 1 cut(s) 1208
SsiI CCGC 2 cut(s) 625, 710
SspI AATATT 1 cut(s) 441
SspMI CTAG 1 cut(s) 810
SstI GAGCTC 1 cut(s) 435
StuI AGGCCT 1 cut(s) 1208
StyD4I CCNGG 2 cut(s) 401, 967
TaaI ACNGT 1 cut(s) 326
TaqI TCGA 3 cut(s) 185, 244, 1254
TasI AATT 6 cut(s) 180, 534, 619, 686, 833, 1157
TfiI GAWTC 3 cut(s) 803, 1142, 1235
Tru1I TTAA 6 cut(s) 419, 533, 689, 822, 947, 1128
Tru9I TTAA 6 cut(s) 419, 533, 689, 822, 947, 1128
TscAI CASTG 3 cut(s) 46, 156, 394
TseFI GTSAC 3 cut(s) 302, 568, 652
TseI GCWGC 1 cut(s) 236
Tsp45I GTSAC 3 cut(s) 302, 568, 652
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 3 cut(s) 46, 156, 394
VpaK11BI GGWCC 1 cut(s) 1221
XapI RAATTY 2 cut(s) 180, 1157
XceI RCATGY 3 cut(s) 503, 722, 1117
XmnI GAANNNNTTC 1 cut(s) 547
XspI CTAG 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.