FvH4_6g10510
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001456
Physical Location & Seq
Forward (+)
6310959 .. 6313664
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10510.t8

Sequence Viewer

Length: 1554 bp
ATGACTTCTTCCTTCACTCACCTCCTCACAAGCAACATGAACATGAACACCAACAACTTTGACCAGGACCGAACCGGCAGCTGGAACTGGGGTCTCTCCGACCGTTCAACTGAGCAACAAGAACACCCCAAGTTCAAGTCACACCAGCCGCCTTCTCTCCCGCTCTCTCCTCCTCCTCCTTCCCCTTCTTCTTACTTGGTTAACGCTTTCAGCCCTACTGATTTTCTCAGCTCCCCCATGTTCCTAGCTAACTCTAATACGCTTCCGTCTCCGACGACCGGAGCCTTTTCGAGTCAGATCTTCGACTGGATGAGCAGCTCGAAAGAGAATAATAGCCAACAAGGAGCGGAGAAGGAGCAGAAAATGTTCACTGATTTCTCATTCCAACCGGCGACATCATCTTCATCGTTCAGCCATCAACAACAATCTTCATCAAGCATGGTTTCATCAGTGGAGGAATCATTGAAAAGTCAACAGCAGCAAACGTGGGGTTTCAACAAAACCTCAAGGCAAGAAGAAACTGTAGCAGAGAAAACAGAAGTAAAATCCGAAGTTGCCCCGGTTCAGAGCTTCAATGCTGCTCCAAAGTCTGAATACACAAATTGCAGCACTCATTCGACTCAATATGTGAGGGAACAGAAATCAGATGATGGATACAATTGGAGAAAATATGGACAGAAGCAAGTGAAAGGTAGCGAAAATCCACGTAGTTATTACAAGTGTACATTTCCCAATTGTCCAACAAAGAAGAAGGTTGAGAGATCATTGGATGGACAGATTACTCAAATTGTGTACAAGGGTAGTCACAACCATGCTAAGCCTCAGTCGACAAGACGATCATCCTCTCAACAACAATCACTTCAAACAAGTTCGTATGCGATTTCTGATCAGTCTGCCTCGACAATATACAATCCAAAAATTGAAGCCGTCACGCTGCAGGAGGACTCTAACTCTGCCTCAATGGGAGGAGAGGATGAGTTTGAGCAAAATTCGCCAATAAGTAACTCAAATGGAGCTGATGATGACAATGAGCCTGAGGCCAAAAGATGGAAAGGAGAAAATACAAACGAGTCATATGCAGCTCCTGGAAGTAGAATTGTGAAAGAACCTAGAATTGTGGTGCAGACCACAAGTGAGATTGATATTCTGGATGACGGATATAGGTGGAGAAAATATGGACAGAAAGTAGTGAAGGGAAATCCAAACCCAAGGAGCTACTACAAATGTACTTCTACTGGCTGTCCAGTGAGGAAACATGTGGAACGAGCATCTCACGATATGAGGGCTGTGATCACAACTTACGAAGGGAAGCACAACCATGATGTTCCTGCAGCGCGCGGCAGTTGTGGTTACGGTGCCTCTAGACCTGCTTCTGATACTAACGGCAACAGCAATGTATCAGTGGCAGCTGTGAGGCCCTTGGCCTTGCCTAATCATTCTAACAATTTGAGCTACCTAAGCTCCCTTCAGAAGCAGCCAACATCAGAGAGCCAATCACCTTATACTCTCAAAATGTTGCAGAACACAGGAAGTTATGGATACTCTGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
WRKY

Protein Analysis

518

Amino Acids

57.46

Weight (kDa)

7.63

Isoelectric Point (pI)

62.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 113 - 168 3.2e-26 WRKY DNA -binding domain
WRKY PF03106 281 - 338 1.6e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1375
AccB1I GGYRCC 1 cut(s) 1355
AccB7I CCANNNNNTGG 1 cut(s) 1047
AccBSI CCGCTC 2 cut(s) 163, 347
AccI GTMKAC 1 cut(s) 827
AccII CGCG 2 cut(s) 1336, 1338
AciI CCGC 4 cut(s) 149, 161, 347, 1338
AcsI RAATTY 1 cut(s) 988
AcuI CTGAAG 1 cut(s) 1451
AfaI GTAC 3 cut(s) 724, 794, 1228
AfiI CCNNNNNNNGG 3 cut(s) 81, 278, 1047
AflIII ACRYGT 1 cut(s) 1255
AgsI TTSAA 7 cut(s) 108, 136, 466, 496, 574, 863, 923
AjnI CCWGG 2 cut(s) 63, 1084
Alw26I GTCTC 2 cut(s) 98, 273
AlwNI CAGNNNCTG 1 cut(s) 1085
AoxI GGCC 3 cut(s) 1038, 1415, 1422
ApoI RAATTY 1 cut(s) 988
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 2 cut(s) 1336, 1338
AspS9I GGNCC 2 cut(s) 67, 1416
AsuC2I CCSGG 1 cut(s) 560
AsuHPI GGTGA 2 cut(s) 11, 1488
AvaII GGWCC 1 cut(s) 67
AxyI CCTNAGG 1 cut(s) 1035
BanI GGYRCC 1 cut(s) 1355
BccI CCATC 4 cut(s) 423, 644, 764, 1041
BceAI ACGGC 2 cut(s) 911, 1399
BcgI CGANNNNNNTGC 2 cut(s) 1058, 1092
BciT130I CCWGG 2 cut(s) 65, 1086
BciVI GTATCC 2 cut(s) 647, 1532
BclI TGATCA 2 cut(s) 886, 1290
BcnI CCSGG 1 cut(s) 560
BcoDI GTCTC 2 cut(s) 98, 273
BfaI CTAG 3 cut(s) 245, 1110, 1362
BfmI CTRYAG 3 cut(s) 522, 935, 1329
BfuAI ACCTGC 1 cut(s) 1375
BfuI GTATCC 2 cut(s) 647, 1532
BglII AGATCT 1 cut(s) 297
BlpI GCTNAGC 1 cut(s) 816
Bme1390I CCNGG 3 cut(s) 65, 560, 1086
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 2 cut(s) 67, 1416
BmiI GGNNCC 2 cut(s) 283, 1357
BmrFI CCNGG 3 cut(s) 65, 560, 1086
BmrI ACTGGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 1277
BmuI ACTGGG 1 cut(s) 97
Bpu10I CCTNAGC 1 cut(s) 1457
Bpu1102I GCTNAGC 1 cut(s) 816
BpuEI CTTGAG 1 cut(s) 490
BpuMI CCSGG 1 cut(s) 560
BsaAI YACGTR 1 cut(s) 707
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 558, 1208, 1419
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 1445, 1475
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 278, 1047
Bse118I RCCGGY 2 cut(s) 74, 388
Bse1I ACTGG 4 cut(s) 92, 311, 1240, 1244
Bse21I CCTNAGG 1 cut(s) 1035
Bse3DI GCAATG 1 cut(s) 1399
BseBI CCWGG 2 cut(s) 65, 1086
BseDI CCNNGG 3 cut(s) 558, 1208, 1419
BseGI GGATG 5 cut(s) 315, 775, 839, 979, 1156
BseLI CCNNNNNNNGG 3 cut(s) 81, 278, 1047
BseMI GCAATG 1 cut(s) 1399
BseMII CTCAG 4 cut(s) 102, 241, 836, 1026
BseNI ACTGG 4 cut(s) 92, 311, 1240, 1244
BsePI GCGCGC 1 cut(s) 1334
BseRI GAGGAG 5 cut(s) 14, 159, 162, 165, 981
BsgI GTGCAG 1 cut(s) 1142
Bsh1236I CGCG 2 cut(s) 1336, 1338
Bsh1285I CGRYCG 2 cut(s) 103, 279
BshFI GGCC 3 cut(s) 1040, 1417, 1424
BshNI GGYRCC 1 cut(s) 1355
BsiEI CGRYCG 2 cut(s) 103, 279
BsiSI CCGG 4 cut(s) 75, 279, 389, 560
BslI CCNNNNNNNGG 3 cut(s) 81, 278, 1047
BsmAI GTCTC 2 cut(s) 98, 273
BsmBI CGTCTC 1 cut(s) 273
BsnI GGCC 3 cut(s) 1040, 1417, 1424
Bso31I GGTCTC 1 cut(s) 98
Bsp1407I TGTACA 2 cut(s) 722, 792
Bsp143I GATC 5 cut(s) 297, 761, 836, 886, 1290
Bsp1720I GCTNAGC 1 cut(s) 816
BspACI CCGC 4 cut(s) 149, 161, 347, 1338
BspANI GGCC 3 cut(s) 1040, 1417, 1424
BspCNI CTCAG 4 cut(s) 103, 240, 835, 1027
BspFNI CGCG 2 cut(s) 1336, 1338
BspLI GGNNCC 2 cut(s) 283, 1357
BspMAI CTGCAG 2 cut(s) 939, 1333
BspMI ACCTGC 1 cut(s) 1375
BspT107I GGYRCC 1 cut(s) 1355
BspTNI GGTCTC 1 cut(s) 98
BsrBI CCGCTC 2 cut(s) 163, 347
BsrDI GCAATG 1 cut(s) 1399
BsrFI RCCGGY 2 cut(s) 74, 388
BsrGI TGTACA 2 cut(s) 722, 792
BsrI ACTGG 4 cut(s) 92, 311, 1240, 1244
BssAI RCCGGY 2 cut(s) 74, 388
BssECI CCNNGG 3 cut(s) 558, 1208, 1419
BssHII GCGCGC 1 cut(s) 1334
BssMI GATC 5 cut(s) 297, 761, 836, 886, 1290
BssT1I CCWWGG 2 cut(s) 1208, 1419
Bst2UI CCWGG 2 cut(s) 65, 1086
Bst4CI ACNGT 3 cut(s) 104, 523, 1355
BstAUI TGTACA 2 cut(s) 722, 792
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 1 cut(s) 1336
BstDEI CTNAG 6 cut(s) 111, 227, 816, 822, 1035, 1457
BstF5I GGATG 5 cut(s) 315, 775, 839, 979, 1156
BstFNI CGCG 2 cut(s) 1336, 1338
BstHHI GCGC 2 cut(s) 1336, 1338
BstKTI GATC 5 cut(s) 300, 764, 839, 889, 1293
BstMAI GTCTC 2 cut(s) 98, 273
BstMBI GATC 5 cut(s) 297, 761, 836, 886, 1290
BstMCI CGRYCG 2 cut(s) 103, 279
BstMWI GCNNNNNNNGC 2 cut(s) 991, 1458
BstNI CCWGG 2 cut(s) 65, 1086
BstNSI RCATGY 1 cut(s) 1259
BstSCI CCNGG 3 cut(s) 63, 558, 1084
BstSFI CTRYAG 3 cut(s) 522, 935, 1329
BstUI CGCG 2 cut(s) 1336, 1338
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
Bsu36I CCTNAGG 1 cut(s) 1035
BsuI GTATCC 2 cut(s) 647, 1532
BsuRI GGCC 3 cut(s) 1040, 1417, 1424
BtsCI GGATG 5 cut(s) 315, 775, 839, 979, 1156
BtsIMutI CAGTG 4 cut(s) 369, 456, 1251, 1407
BveI ACCTGC 1 cut(s) 1375
Cac8I GCNNGC 1 cut(s) 1336
CaiI CAGNNNCTG 1 cut(s) 1085
CfoI GCGC 2 cut(s) 1336, 1338
Cfr10I RCCGGY 2 cut(s) 74, 388
Cfr13I GGNCC 2 cut(s) 67, 1416
Csp6I GTAC 3 cut(s) 723, 793, 1227
CviAII CATG 7 cut(s) 37, 43, 238, 439, 812, 1256, 1319
CviQI GTAC 3 cut(s) 723, 793, 1227
DdeI CTNAG 6 cut(s) 111, 227, 816, 822, 1035, 1457
DpnI GATC 5 cut(s) 299, 763, 838, 888, 1292
DpnII GATC 5 cut(s) 297, 761, 836, 886, 1290
Eco130I CCWWGG 2 cut(s) 1208, 1419
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 1451
Eco81I CCTNAGG 1 cut(s) 1035
EcoO109I RGGNCCY 1 cut(s) 1416
EcoRII CCWGG 2 cut(s) 63, 1084
EcoT14I CCWWGG 2 cut(s) 1208, 1419
ErhI CCWWGG 2 cut(s) 1208, 1419
Esp3I CGTCTC 1 cut(s) 273
FaeI CATG 7 cut(s) 40, 46, 241, 442, 815, 1259, 1322
FatI CATG 7 cut(s) 36, 42, 237, 438, 811, 1255, 1318
FauI CCCGC 1 cut(s) 168
FauNDI CATATG 1 cut(s) 1075
FbaI TGATCA 2 cut(s) 886, 1290
FblI GTMKAC 1 cut(s) 827
FokI GGATG 5 cut(s) 322, 782, 826, 986, 1163
FspBI CTAG 3 cut(s) 245, 1110, 1362
GlaI GCGC 2 cut(s) 1335, 1337
HaeIII GGCC 3 cut(s) 1040, 1417, 1424
HapII CCGG 4 cut(s) 75, 279, 389, 560
HhaI GCGC 2 cut(s) 1336, 1338
Hin1II CATG 7 cut(s) 40, 46, 241, 442, 815, 1259, 1322
Hin6I GCGC 2 cut(s) 1334, 1336
HinP1I GCGC 2 cut(s) 1334, 1336
HincII GTYRAC 3 cut(s) 202, 473, 828
HindII GTYRAC 3 cut(s) 202, 473, 828
HinfI GANTC 5 cut(s) 292, 458, 619, 944, 1070
HpaI GTTAAC 1 cut(s) 202
HpaII CCGG 4 cut(s) 75, 279, 389, 560
HphI GGTGA 2 cut(s) 11, 1488
Hpy166II GTNNAC 6 cut(s) 202, 369, 473, 723, 793, 828
Hpy188III TCNNGA 3 cut(s) 1148, 1274, 1362
Hpy8I GTNNAC 6 cut(s) 202, 369, 473, 723, 793, 828
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 9 cut(s) 22, 162, 189, 195, 346, 745, 1186, 1298, 1475
HpyCH4III ACNGT 3 cut(s) 104, 523, 1355
HpyCH4IV ACGT 2 cut(s) 485, 706
HpyCH4V TGCA 6 cut(s) 606, 937, 1079, 1123, 1331, 1519
HpyF10VI GCNNNNNNNGC 2 cut(s) 991, 1458
HpyF3I CTNAG 6 cut(s) 111, 227, 816, 822, 1035, 1457
HpySE526I ACGT 2 cut(s) 485, 706
Hsp92II CATG 7 cut(s) 40, 46, 241, 442, 815, 1259, 1322
HspAI GCGC 2 cut(s) 1334, 1336
Ksp22I TGATCA 2 cut(s) 886, 1290
KspAI GTTAAC 1 cut(s) 202
Kzo9I GATC 5 cut(s) 297, 761, 836, 886, 1290
LmnI GCTCC 9 cut(s) 236, 281, 344, 355, 586, 1013, 1087, 1212, 1466
LweI GCATC 1 cut(s) 1277
MaeI CTAG 3 cut(s) 245, 1110, 1362
MaeII ACGT 2 cut(s) 485, 706
MaeIII GTNAC 5 cut(s) 138, 803, 928, 1001, 1349
MalI GATC 5 cut(s) 299, 763, 838, 888, 1292
MbiI CCGCTC 2 cut(s) 163, 347
MboI GATC 5 cut(s) 297, 761, 836, 886, 1290
MboII GAAGA 6 cut(s) 180, 292, 393, 420, 527, 760
MfeI CAATTG 2 cut(s) 658, 733
MflI RGATCY 1 cut(s) 297
MluCI AATT 9 cut(s) 601, 658, 733, 786, 918, 988, 1095, 1113, 1444
MlyI GAGTC 4 cut(s) 301, 613, 938, 1079
MmeI TCCRAC 4 cut(s) 123, 296, 409, 764
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 1 cut(s) 201
MslI CAYNNNNRTG 3 cut(s) 41, 810, 1317
MspA1I CMGCKG 2 cut(s) 81, 1409
MspI CCGG 4 cut(s) 75, 279, 389, 560
MspR9I CCNGG 3 cut(s) 65, 560, 1086
MunI CAATTG 2 cut(s) 658, 733
MvaI CCWGG 2 cut(s) 65, 1086
MvnI CGCG 2 cut(s) 1336, 1338
MwoI GCNNNNNNNGC 2 cut(s) 991, 1458
NciI CCSGG 1 cut(s) 560
NdeI CATATG 1 cut(s) 1075
NdeII GATC 5 cut(s) 297, 761, 836, 886, 1290
NlaIII CATG 7 cut(s) 40, 46, 241, 442, 815, 1259, 1322
NlaIV GGNNCC 2 cut(s) 283, 1357
NmuCI GTSAC 3 cut(s) 138, 803, 928
NspI RCATGY 1 cut(s) 1259
PauI GCGCGC 1 cut(s) 1334
PciI ACATGT 1 cut(s) 1255
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 458
PflMI CCANNNNNTGG 1 cut(s) 1047
PfoI TCCNGGA 1 cut(s) 1084
PleI GAGTC 4 cut(s) 300, 613, 938, 1078
PpsI GAGTC 4 cut(s) 300, 613, 938, 1078
Ppu21I YACGTR 1 cut(s) 707
PscI ACATGT 1 cut(s) 1255
Psp6I CCWGG 2 cut(s) 63, 1084
PspGI CCWGG 2 cut(s) 63, 1084
PspN4I GGNNCC 2 cut(s) 283, 1357
PspPI GGNCC 2 cut(s) 67, 1416
PstI CTGCAG 2 cut(s) 939, 1333
PstNI CAGNNNCTG 1 cut(s) 1085
PsuI RGATCY 1 cut(s) 297
PteI GCGCGC 1 cut(s) 1334
PvuII CAGCTG 2 cut(s) 81, 1409
RsaI GTAC 3 cut(s) 724, 794, 1228
RsaNI GTAC 3 cut(s) 723, 793, 1227
RseI CAYNNNNRTG 3 cut(s) 41, 810, 1317
SalI GTCGAC 1 cut(s) 826
SaqAI TTAA 1 cut(s) 201
Sau3AI GATC 5 cut(s) 297, 761, 836, 886, 1290
Sau96I GGNCC 2 cut(s) 67, 1416
SchI GAGTC 4 cut(s) 301, 613, 938, 1079
ScrFI CCNGG 3 cut(s) 65, 560, 1086
SfaNI GCATC 1 cut(s) 1277
SfcI CTRYAG 3 cut(s) 522, 935, 1329
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 3 cut(s) 41, 810, 1317
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 9 cut(s) 601, 658, 733, 786, 918, 988, 1095, 1113, 1444
SsiI CCGC 4 cut(s) 149, 161, 347, 1338
SspMI CTAG 3 cut(s) 245, 1110, 1362
StyD4I CCNGG 3 cut(s) 63, 558, 1084
StyI CCWWGG 2 cut(s) 1208, 1419
TaaI ACNGT 3 cut(s) 104, 523, 1355
TaiI ACGT 2 cut(s) 488, 709
TaqI TCGA 6 cut(s) 290, 303, 320, 617, 827, 899
TaqII GACCGA 1 cut(s) 84
TasI AATT 9 cut(s) 601, 658, 733, 786, 918, 988, 1095, 1113, 1444
TatI WGTACW 3 cut(s) 722, 792, 1226
TauI GCSGC 2 cut(s) 151, 1341
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 4 cut(s) 376, 456, 1251, 1407
TseFI GTSAC 3 cut(s) 138, 803, 928
Tsp45I GTSAC 3 cut(s) 138, 803, 928
TspDTI ATGAA 5 cut(s) 53, 59, 393, 420, 435
TspGWI ACGGA 2 cut(s) 255, 1170
TspRI CASTG 4 cut(s) 376, 456, 1251, 1407
Van91I CCANNNNNTGG 1 cut(s) 1047
VpaK11BI GGWCC 1 cut(s) 67
XapI RAATTY 1 cut(s) 988
XbaI TCTAGA 1 cut(s) 1361
XceI RCATGY 1 cut(s) 1259
XmiI GTMKAC 1 cut(s) 827
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 3 cut(s) 245, 1110, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.