Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0001456

Physical Location & Seq

Genomic Coordinates Reverse (-)

3557934 .. 3560487

Gene Structure 2554 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g04580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGCCTCCTCTTCAGGGAGCTTAGAAACCTCAGCAAACTCACACCCAGCAGCCTTCACTTTCTCAACGCACCCATTCATGACCAACTCCTTCTCCGACCTCTTAGAAGCCGGCACAGATGAGTACTCGAACCCACCTAGATACATCGGCCAGGGCGGGCTATCGGATCGAATTGCAGAGCGTACTGGGTCCGGCGTACCCAAATTCAAGTCCCTACCGCCGCCCTCACTTCCCATTTCTCCTCCCTCTATATCTCCATCTTCCTACTTTGCTATCCCACCTGGGTTGAGCCCAGCTGAGCTTCTTGACTCTCCTGTTCTCCTAAACGCTTCAAACATTCTTCCATCTCCAACTACTGGAAGCTTTGCAGCTCGTGGGGCCTTCAATTGGAAGAACAACCAACAGAACGTAAAGCAGGAGAGCAAGAACCACTCAGACTTCTCTTTCCAAACCCAAGCAAGACCTCCCATTTCTTCATCATCGTCAATGTTTCAATCCTCAAACACCTCAATTCAAACTACACAAGAGCAGGCATGCAACAACAATTACTTTCCGGCTCAAGAACTCCCAAAGCAAGAATATGGTTCTGTTCAGACCTTGTCATCTGAATTGACCACCAAAACTCTCCAAAGTAATGCTCCGGCGAATGGCGGTTTCAACCAGCAATCTCAGACCTTGAGCAGGAAGTCGGATGATGGTTTCAATTGGAGAAAATATGGTCAAAAACAAGTGAAGGGAAGTGAAAATCCCAGAAGCTATTACAAGTGCACTTACCCTAATTGCCCAACTAAGAAAAAAGTAGAGAGGTCGTTGGATGGACAAATCACTGAGATTGTTTACAAGGGCAATCACAACCATCCCAAGCCACAGAACCCTAGAAGATCATCATCAAATTCTCATGCAATTCATGCTTTCAATCCCACCAACACCAATGAAATCCCAGATCAAACTTATGCTAATCATGGCAATTCACAAATGGATTCCATTGGAACTCCTGAGCATTCATCGATATCGATCGGGGACGATGATTTTGAGCAGAGCTCTCAGAGGAGCAAGTCAGGAGGAGGGGAGGAGTTTGATGAAGATGAACCTAATGCAAAAAGATGGAAAAATGAAGCTGATCGCAATGAAGGAATTTCAGCACCTGGGAGTAGAACAGTGAGAGAGCCTAGAGTTGTAGTTCAGACAACTAGTGACATTGATATTCTAGATGATGGATACAGATGGAGGAAGTACGGTCAGAAAGTGGTAAAAGGCAACCCAAATCCAAGGAGCTACTACAAGTGCACAAATCCAGGATGTCCAGTGAGAAAGCATGTTGAGAGAGCTTCTCACGATCTTAGAGCAGTGATCACAACCTATGAAGGGAAGCACAACCACGACGTCCCTGCCGCTCGTGGCAGCAGCAGCCATGCTGCTGTCAATAGGCCTATACCAAACAACAACAACAATGTAGCTTCAGCAATGAGGCCTATAACTCACCACACTAACAATTCTGCTAATACCAACTCTCTTCAAAACTTGAGGCAACCAACATCAGAAGGGCATGCTCCCTTTAGCCTAGAGATGTTGCAGAGCCCGGGAAGTTACGGATTCGTGGGGTTCGACGGAAACTCCATGGGATCATACATGAACCAAACACAGCTTAGTGACATATTTTCTAAAGCCAAGGAAGAGCCAAGGGATGATGCATTTTTTGAATCATTGCTGTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009700 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010506 GO:0010508 GO:0010556 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0031323 GO:0031325 GO:0031326 GO:0031329 GO:0031331 GO:0033554 GO:0034605 GO:0034641 GO:0042221 GO:0042430 GO:0042435 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044424 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0070370 GO:0071704 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

572

Amino Acids

62.68

Weight (kDa)

7.29

Isoelectric Point (pI)

54.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	231 - 287	1.4e-26	WRKY DNA -binding domain
WRKY	PF03106	404 - 461	2.1e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001456)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07100 AT5G07100 AT5G07100 AT5G07100 AT5G07100
fragaria_vesca	FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_3g39850 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510 FvH4_6g10510
malus_domestica	MD03G1057400.v1.1 MD04G1167700.v1.1 MD06G1115200.v1.1 MD11G1059400.v1.1 MD12G1181000.v1.1
prunus_persica	Prupe.6G046900_v2.0.a1 Prupe.6G286000_v2.0.a1
pyrus_communis	pycom03g04580 pycom04g14890 pycom06g10790 pycom11g04930 pycom12g17060
rosa_chinensis	RchiOBHm_Chr3g0461481 RchiOBHm_Chr5g0071811
rosa_laevigata	RLG00000024931 RLG00000036250
rosa_multiflora	Rmu_sc0002677.1_g000005 Rmu_ssc0000127.1_g000054
rosa_roxburghii	Rroxscaffold_1G00009350 Rroxscaffold_6G00418250 Rroxscaffold_6G00418580
rosa_rugosa	Rorug03G0051000 Rorug03G0051100.1 Rorug05G0414700
rosa_samantha	Rh3AG108800 Rh3BG112700 Rh3CG114200 Rh3DG114300 Rh5AG471000 Rh5BG490700 Rh5CG514700 Rh5DG502000
rosa_wichuraiana	Rw3G009250 Rw5G043770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1386
AccB7I	CCANNNNNTGG	1 cut(s)	356
AccBSI	CCGCTC	1 cut(s)	1394
AciI	CCGC	5 cut(s)	156, 218, 221, 651, 1392
AclWI	GGATC	2 cut(s)	174, 1630
AcoI	YGGCCR	1 cut(s)	148
AcsI	RAATTY	3 cut(s)	203, 892, 1134
AcuI	CTGAAG	1 cut(s)	1443
AcyI	GRCGYC	1 cut(s)	1383
AfaI	GTAC	4 cut(s)	125, 184, 198, 1235
AfiI	CCNNNNNNNGG	6 cut(s)	15, 356, 387, 647, 681, 1365
AhlI	ACTAGT	1 cut(s)	1190
AjnI	CCWGG	4 cut(s)	150, 280, 1144, 1294
Alw21I	GWGCWC	3 cut(s)	770, 1043, 1289
Alw44I	GTGCAC	2 cut(s)	766, 1285
AlwI	GGATC	2 cut(s)	174, 1630
AlwNI	CAGNNNCTG	1 cut(s)	1145
Ama87I	CYCGRG	1 cut(s)	1579
AoxI	GGCC	5 cut(s)	3, 148, 378, 1427, 1469
ApaLI	GTGCAC	2 cut(s)	766, 1285
ApeKI	GCWGC	6 cut(s)	50, 368, 1401, 1404, 1407, 1415
ApoI	RAATTY	3 cut(s)	203, 892, 1134
ArsI	GACNNNNNNTTYG	2 cut(s)	1013, 1045
AspS9I	GGNCC	2 cut(s)	189, 378
AsuC2I	CCSGG	2 cut(s)	1580, 1581
AsuHPI	GGTGA	1 cut(s)	1472
AvaI	CYCGRG	1 cut(s)	1579
AvaII	GGWCC	1 cut(s)	189
BaeGI	GKGCMC	2 cut(s)	770, 1289
BanII	GRGCYC	3 cut(s)	293, 1043, 1580
BauI	CACGAG	2 cut(s)	372, 1395
Bbv12I	GWGCWC	3 cut(s)	770, 1043, 1289
BbvCI	CCTCAGC	1 cut(s)	31
BbvI	GCAGC	6 cut(s)	62, 380, 1402, 1413, 1416, 1419
BccI	CCATC	8 cut(s)	265, 352, 689, 809, 864, 1098, 1208, 1218
BcgI	CGANNNNNNTGC	2 cut(s)	1370, 1404
BciT130I	CCWGG	4 cut(s)	152, 282, 1146, 1296
BciVI	GTATCC	1 cut(s)	1211
BclI	TGATCA	1 cut(s)	1350
BcnI	CCSGG	2 cut(s)	1580, 1581
BcuI	ACTAGT	1 cut(s)	1190
BfaI	CTAG	6 cut(s)	138, 876, 1170, 1191, 1208, 1562
BfuI	GTATCC	1 cut(s)	1211
BisI	GCNGC	8 cut(s)	51, 221, 369, 1392, 1402, 1405, 1408, 1416
BlpI	GCTNAGC	1 cut(s)	297
BlsI	GCNGC	8 cut(s)	52, 222, 370, 1393, 1403, 1406, 1409, 1417
BmcAI	AGTACT	1 cut(s)	125
Bme1390I	CCNGG	6 cut(s)	152, 282, 1146, 1296, 1580, 1581
Bme18I	GGWCC	1 cut(s)	189
BmeT110I	CYCGRG	1 cut(s)	1579
BmgT120I	GGNCC	2 cut(s)	189, 378
BmiI	GGNNCC	2 cut(s)	190, 379
BmrFI	CCNGG	6 cut(s)	152, 282, 1146, 1296, 1580, 1581
BmrI	ACTGGG	1 cut(s)	195
BmsI	GCATC	1 cut(s)	1678
BmuI	ACTGGG	1 cut(s)	195
BplI	GAGNNNNNCTC	4 cut(s)	1025, 1057, 1315, 1347
Bpu10I	CCTNAGC	2 cut(s)	31, 996
Bpu1102I	GCTNAGC	1 cut(s)	297
BpuEI	CTTGAG	3 cut(s)	543, 697, 1543
BpuMI	CCSGG	2 cut(s)	1580, 1581
Bsa29I	ATCGAT	2 cut(s)	1007, 1013
BsaBI	GATNNNNATC	2 cut(s)	886, 1013
BsaHI	GRCGYC	1 cut(s)	1383
BsaJI	CCNNGG	8 cut(s)	151, 281, 1145, 1268, 1579, 1617, 1668, 1679
BsaXI	ACNNNNNCTCC	4 cut(s)	77, 107, 1598, 1628
Bsc4I	CCNNNNNNNGG	6 cut(s)	15, 356, 387, 647, 681, 1365
Bse118I	RCCGGY	1 cut(s)	110
Bse1I	ACTGG	3 cut(s)	190, 361, 1304
Bse3DI	GCAATG	3 cut(s)	1132, 1470, 1703
Bse8I	GATNNNNATC	2 cut(s)	886, 1013
BseBI	CCWGG	4 cut(s)	152, 282, 1146, 1296
BseCI	ATCGAT	2 cut(s)	1007, 1013
BseDI	CCNNGG	8 cut(s)	151, 281, 1145, 1268, 1579, 1617, 1668, 1679
BseGI	GGATG	5 cut(s)	697, 820, 856, 1304, 1690
BseJI	GATNNNNATC	2 cut(s)	886, 1013
BseLI	CCNNNNNNNGG	6 cut(s)	15, 356, 387, 647, 681, 1365
BseMI	GCAATG	3 cut(s)	1132, 1470, 1703
BseMII	CTCAG	7 cut(s)	45, 288, 447, 683, 819, 987, 1058
BseNI	ACTGG	3 cut(s)	190, 361, 1304
BseRI	GAGGAG	4 cut(s)	231, 1063, 1077, 1085
BseSI	GKGCMC	2 cut(s)	770, 1289
BseXI	GCAGC	6 cut(s)	62, 380, 1402, 1413, 1416, 1419
BseYI	CCCAGC	2 cut(s)	46, 292
Bsh1285I	CGRYCG	1 cut(s)	1017
BshFI	GGCC	5 cut(s)	5, 150, 380, 1429, 1471
BshVI	ATCGAT	2 cut(s)	1007, 1013
BsiEI	CGRYCG	1 cut(s)	1017
BsiHKAI	GWGCWC	3 cut(s)	770, 1043, 1289
BsiHKCI	CYCGRG	1 cut(s)	1579
BsiSI	CCGG	5 cut(s)	111, 192, 554, 641, 1580
BslFI	GGGAC	3 cut(s)	196, 1034, 1370
BslI	CCNNNNNNNGG	6 cut(s)	15, 356, 387, 647, 681, 1365
BsmFI	GGGAC	3 cut(s)	196, 1034, 1370
BsmI	GAATGC	1 cut(s)	1000
BsnI	GGCC	5 cut(s)	5, 150, 380, 1429, 1471
BsoBI	CYCGRG	1 cut(s)	1579
Bsp1286I	GDGCHC	5 cut(s)	293, 770, 1043, 1289, 1580
Bsp143I	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
Bsp1720I	GCTNAGC	1 cut(s)	297
Bsp19I	CCATGG	1 cut(s)	1617
BspACI	CCGC	5 cut(s)	156, 218, 221, 651, 1392
BspANI	GGCC	5 cut(s)	5, 150, 380, 1429, 1471
BspCNI	CTCAG	7 cut(s)	44, 289, 446, 682, 820, 988, 1057
BspDI	ATCGAT	2 cut(s)	1007, 1013
BspHI	TCATGA	1 cut(s)	78
BspLI	GGNNCC	2 cut(s)	190, 379
BspPI	GGATC	2 cut(s)	174, 1630
BspQI	GCTCTTC	1 cut(s)	1668
BsrBI	CCGCTC	1 cut(s)	1394
BsrDI	GCAATG	3 cut(s)	1132, 1470, 1703
BsrFI	RCCGGY	1 cut(s)	110
BsrI	ACTGG	3 cut(s)	190, 361, 1304
BssAI	RCCGGY	1 cut(s)	110
BssECI	CCNNGG	8 cut(s)	151, 281, 1145, 1268, 1579, 1617, 1668, 1679
BssMI	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
BssNI	GRCGYC	1 cut(s)	1383
BssSI	CACGAG	2 cut(s)	372, 1395
BssT1I	CCWWGG	4 cut(s)	1268, 1617, 1668, 1679
Bst2BI	CACGAG	2 cut(s)	372, 1395
Bst2UI	CCWGG	4 cut(s)	152, 282, 1146, 1296
Bst4CI	ACNGT	2 cut(s)	1159, 1238
Bst6I	CTCTTC	3 cut(s)	16, 1518, 1668
BstACI	GRCGYC	1 cut(s)	1383
BstAPI	GCANNNNNTGC	1 cut(s)	908
BstC8I	GCNNGC	5 cut(s)	112, 158, 531, 535, 1548
BstDSI	CCRYGG	1 cut(s)	1617
BstENI	CCTNNNNNAGG	1 cut(s)	679
BstF5I	GGATG	5 cut(s)	697, 820, 856, 1304, 1690
BstKTI	GATC	8 cut(s)	169, 884, 946, 1017, 1123, 1339, 1353, 1625
BstMBI	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
BstMCI	CGRYCG	1 cut(s)	1017
BstMWI	GCNNNNNNNGC	3 cut(s)	377, 908, 1407
BstNI	CCWGG	4 cut(s)	152, 282, 1146, 1296
BstNSI	RCATGY	3 cut(s)	537, 1319, 1550
BstSCI	CCNGG	6 cut(s)	150, 280, 1144, 1294, 1578, 1579
BstSLI	GKGCMC	2 cut(s)	770, 1289
BstV1I	GCAGC	6 cut(s)	62, 380, 1402, 1413, 1416, 1419
Bsu15I	ATCGAT	2 cut(s)	1007, 1013
BsuI	GTATCC	1 cut(s)	1211
BsuRI	GGCC	5 cut(s)	5, 150, 380, 1429, 1471
BsuTUI	ATCGAT	2 cut(s)	1007, 1013
BtgI	CCRYGG	1 cut(s)	1617
BtsCI	GGATG	5 cut(s)	697, 820, 856, 1304, 1690
BtsI	GCAGTG	1 cut(s)	1353
BtsIMutI	CAGTG	4 cut(s)	825, 1164, 1311, 1353
Cac8I	GCNNGC	5 cut(s)	112, 158, 531, 535, 1548
CaiI	CAGNNNCTG	1 cut(s)	1145
CciI	TCATGA	1 cut(s)	78
Cfr10I	RCCGGY	1 cut(s)	110
Cfr13I	GGNCC	2 cut(s)	189, 378
Cfr9I	CCCGGG	1 cut(s)	1579
ClaI	ATCGAT	2 cut(s)	1007, 1013
Csp6I	GTAC	4 cut(s)	124, 183, 197, 1234
CspCI	CAANNNNNGTGG	2 cut(s)	267, 302
CviQI	GTAC	4 cut(s)	124, 183, 197, 1234
DpnI	GATC	8 cut(s)	168, 883, 945, 1016, 1122, 1338, 1352, 1624
DpnII	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
EaeI	YGGCCR	1 cut(s)	148
Eam1104I	CTCTTC	3 cut(s)	16, 1518, 1668
EarI	CTCTTC	3 cut(s)	16, 1518, 1668
Ecl136II	GAGCTC	1 cut(s)	1041
Eco130I	CCWWGG	4 cut(s)	1268, 1617, 1668, 1679
Eco147I	AGGCCT	2 cut(s)	1429, 1471
Eco24I	GRGCYC	3 cut(s)	293, 1043, 1580
Eco32I	GATATC	1 cut(s)	1011
Eco47I	GGWCC	1 cut(s)	189
Eco53kI	GAGCTC	1 cut(s)	1041
Eco57I	CTGAAG	1 cut(s)	1443
Eco88I	CYCGRG	1 cut(s)	1579
EcoICRI	GAGCTC	1 cut(s)	1041
EcoNI	CCTNNNNNAGG	1 cut(s)	679
EcoO109I	RGGNCCY	1 cut(s)	378
EcoRII	CCWGG	4 cut(s)	150, 280, 1144, 1294
EcoRV	GATATC	1 cut(s)	1011
EcoT14I	CCWWGG	4 cut(s)	1268, 1617, 1668, 1679
EcoT22I	ATGCAT	1 cut(s)	1693
EcoT38I	GRGCYC	3 cut(s)	293, 1043, 1580
ErhI	CCWWGG	4 cut(s)	1268, 1617, 1668, 1679
FaqI	GGGAC	3 cut(s)	196, 1034, 1370
FauI	CCCGC	1 cut(s)	149
FbaI	TGATCA	1 cut(s)	1350
Fnu4HI	GCNGC	8 cut(s)	51, 221, 369, 1392, 1402, 1405, 1408, 1416
FokI	GGATG	5 cut(s)	704, 827, 843, 1311, 1697
FriOI	GRGCYC	3 cut(s)	293, 1043, 1580
Fsp4HI	GCNGC	8 cut(s)	51, 221, 369, 1392, 1402, 1405, 1408, 1416
FspBI	CTAG	6 cut(s)	138, 876, 1170, 1191, 1208, 1562
GluI	GCNGC	8 cut(s)	51, 221, 369, 1392, 1402, 1405, 1408, 1416
GsaI	CCCAGC	2 cut(s)	50, 296
HaeIII	GGCC	5 cut(s)	5, 150, 380, 1429, 1471
HapII	CCGG	5 cut(s)	111, 192, 554, 641, 1580
Hin1I	GRCGYC	1 cut(s)	1383
HindIII	AAGCTT	1 cut(s)	361
HinfI	GANTC	4 cut(s)	308, 980, 1593, 1700
HpaII	CCGG	5 cut(s)	111, 192, 554, 641, 1580
HphI	GGTGA	1 cut(s)	1472
Hpy166II	GTNNAC	3 cut(s)	768, 838, 1287
Hpy188III	TCNNGA	7 cut(s)	79, 305, 560, 995, 1059, 1208, 1334
Hpy8I	GTNNAC	3 cut(s)	768, 838, 1287
Hpy99I	CGWCG	2 cut(s)	1385, 1610
HpyAV	CCTTC	7 cut(s)	64, 100, 391, 727, 1124, 1358, 1535
HpyCH4III	ACNGT	2 cut(s)	1159, 1238
HpyCH4IV	ACGT	2 cut(s)	408, 1383
HpyCH4V	TGCA	9 cut(s)	176, 368, 537, 768, 902, 1097, 1287, 1573, 1691
HpyF10VI	GCNNNNNNNGC	3 cut(s)	377, 908, 1407
HpySE526I	ACGT	2 cut(s)	408, 1383
Hsp92I	GRCGYC	1 cut(s)	1383
KroI	GCCGGC	1 cut(s)	110
KroNI	GCCGGC	1 cut(s)	112
Ksp22I	TGATCA	1 cut(s)	1350
Kzo9I	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
LguI	GCTCTTC	1 cut(s)	1668
LmnI	GCTCC	5 cut(s)	18, 643, 1050, 1272, 1555
Lsp1109I	GCAGC	6 cut(s)	62, 380, 1402, 1413, 1416, 1419
LweI	GCATC	1 cut(s)	1678
MaeI	CTAG	6 cut(s)	138, 876, 1170, 1191, 1208, 1562
MaeII	ACGT	2 cut(s)	408, 1383
MaeIII	GTNAC	3 cut(s)	1193, 1586, 1649
MalI	GATC	8 cut(s)	168, 883, 945, 1016, 1122, 1338, 1352, 1624
MbiI	CCGCTC	1 cut(s)	1394
MboI	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
MboII	GAAGA	9 cut(s)	3, 252, 332, 403, 465, 891, 1094, 1505, 1685
MfeI	CAATTG	2 cut(s)	385, 703
MhlI	GDGCHC	5 cut(s)	293, 770, 1043, 1289, 1580
MlyI	GAGTC	1 cut(s)	302
MmeI	TCCRAC	4 cut(s)	120, 374, 669, 792
Mph1103I	ATGCAT	1 cut(s)	1693
MroNI	GCCGGC	1 cut(s)	110
MslI	CAYNNNNRTG	1 cut(s)	1708
MspA1I	CMGCKG	1 cut(s)	296
MspI	CCGG	5 cut(s)	111, 192, 554, 641, 1580
MspR9I	CCNGG	6 cut(s)	152, 282, 1146, 1296, 1580, 1581
MunI	CAATTG	2 cut(s)	385, 703
Mva1269I	GAATGC	1 cut(s)	1000
MvaI	CCWGG	4 cut(s)	152, 282, 1146, 1296
MwoI	GCNNNNNNNGC	3 cut(s)	377, 908, 1407
NaeI	GCCGGC	1 cut(s)	112
NciI	CCSGG	2 cut(s)	1580, 1581
NcoI	CCATGG	1 cut(s)	1617
NdeII	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
NgoMIV	GCCGGC	1 cut(s)	110
NlaIV	GGNNCC	2 cut(s)	190, 379
NmuCI	GTSAC	2 cut(s)	1193, 1649
NsiI	ATGCAT	1 cut(s)	1693
NspI	RCATGY	3 cut(s)	537, 1319, 1550
PaeI	GCATGC	2 cut(s)	537, 1550
PagI	TCATGA	1 cut(s)	78
PceI	AGGCCT	2 cut(s)	1429, 1471
PciSI	GCTCTTC	1 cut(s)	1668
PcsI	WCGNNNNNNNCGW	1 cut(s)	1602
PctI	GAATGC	1 cut(s)	1000
PdiI	GCCGGC	1 cut(s)	112
PfeI	GAWTC	3 cut(s)	980, 1593, 1700
PflFI	GACNNNGTC	1 cut(s)	598
PflMI	CCANNNNNTGG	1 cut(s)	356
PfoI	TCCNGGA	1 cut(s)	1294
PkrI	GCNGC	8 cut(s)	52, 222, 370, 1393, 1403, 1406, 1409, 1417
Ple19I	CGATCG	1 cut(s)	1017
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
Psp124BI	GAGCTC	1 cut(s)	1043
Psp6I	CCWGG	4 cut(s)	150, 280, 1144, 1294
PspFI	CCCAGC	2 cut(s)	46, 292
PspGI	CCWGG	4 cut(s)	150, 280, 1144, 1294
PspN4I	GGNNCC	2 cut(s)	190, 379
PspPI	GGNCC	2 cut(s)	189, 378
PstNI	CAGNNNCTG	1 cut(s)	1145
PsyI	GACNNNGTC	1 cut(s)	598
PvuI	CGATCG	1 cut(s)	1017
PvuII	CAGCTG	1 cut(s)	296
RsaI	GTAC	4 cut(s)	125, 184, 198, 1235
RsaNI	GTAC	4 cut(s)	124, 183, 197, 1234
RseI	CAYNNNNRTG	1 cut(s)	1708
SacI	GAGCTC	1 cut(s)	1043
SapI	GCTCTTC	1 cut(s)	1668
SatI	GCNGC	8 cut(s)	51, 221, 369, 1392, 1402, 1405, 1408, 1416
Sau3AI	GATC	8 cut(s)	166, 881, 943, 1014, 1120, 1336, 1350, 1622
Sau96I	GGNCC	2 cut(s)	189, 378
ScaI	AGTACT	1 cut(s)	125
SchI	GAGTC	1 cut(s)	302
ScrFI	CCNGG	6 cut(s)	152, 282, 1146, 1296, 1580, 1581
SduI	GDGCHC	5 cut(s)	293, 770, 1043, 1289, 1580
SfaNI	GCATC	1 cut(s)	1678
SinI	GGWCC	1 cut(s)	189
SmaI	CCCGGG	1 cut(s)	1581
SmiMI	CAYNNNNRTG	1 cut(s)	1708
SmlI	CTYRAG	3 cut(s)	558, 676, 1522
SmoI	CTYRAG	3 cut(s)	558, 676, 1522
SpeI	ACTAGT	1 cut(s)	1190
SphI	GCATGC	2 cut(s)	537, 1550
SseBI	AGGCCT	2 cut(s)	1429, 1471
SsiI	CCGC	5 cut(s)	156, 218, 221, 651, 1392
SspMI	CTAG	6 cut(s)	138, 876, 1170, 1191, 1208, 1562
SstI	GAGCTC	1 cut(s)	1043
StuI	AGGCCT	2 cut(s)	1429, 1471
StyD4I	CCNGG	6 cut(s)	150, 280, 1144, 1294, 1578, 1579
StyI	CCWWGG	4 cut(s)	1268, 1617, 1668, 1679
TaaI	ACNGT	2 cut(s)	1159, 1238
TaiI	ACGT	2 cut(s)	411, 1386
TaqI	TCGA	5 cut(s)	128, 169, 1007, 1013, 1605
TatI	WGTACW	1 cut(s)	123
TauI	GCSGC	2 cut(s)	223, 1394
TfiI	GAWTC	3 cut(s)	980, 1593, 1700
TscAI	CASTG	4 cut(s)	832, 1164, 1311, 1353
TseFI	GTSAC	2 cut(s)	1193, 1649
TseI	GCWGC	6 cut(s)	50, 368, 1401, 1404, 1407, 1415
Tsp45I	GTSAC	2 cut(s)	1193, 1649
TspGWI	ACGGA	2 cut(s)	1605, 1623
TspMI	CCCGGG	1 cut(s)	1579
TspRI	CASTG	4 cut(s)	832, 1164, 1311, 1353
Tth111I	GACNNNGTC	1 cut(s)	598
Van91I	CCANNNNNTGG	1 cut(s)	356
VneI	GTGCAC	2 cut(s)	766, 1285
VpaK11BI	GGWCC	1 cut(s)	189
XagI	CCTNNNNNAGG	1 cut(s)	679
XapI	RAATTY	3 cut(s)	203, 892, 1134
XbaI	TCTAGA	1 cut(s)	1207
XceI	RCATGY	3 cut(s)	537, 1319, 1550
XmaI	CCCGGG	1 cut(s)	1579
XspI	CTAG	6 cut(s)	138, 876, 1170, 1191, 1208, 1562
ZraI	GACGTC	1 cut(s)	1384
ZrmI	AGTACT	1 cut(s)	125
Zsp2I	ATGCAT	1 cut(s)	1693

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.